The Ukrainian Biochemical Journal最新文献_第8页

Purification and characterization of platelet aggregation inhibitor from the venom of Bitis arietans 白斑双翅虫毒液中血小板聚集抑制剂的纯化及特性研究

The Ukrainian Biochemical Journal Pub Date : 2022-12-08 DOI: 10.15407/ubj94.05.007

O. Platonov, V. Nikulina, Y. Kucheryavyi, V. Gryshchuk, Y. Stohniy, V. Chernyshenko, O. Slominskyi, A. Rebriev, K. Savchenko, L. Garmanchuk

{"title":"Purification and characterization of platelet aggregation inhibitor from the venom of Bitis arietans","authors":"O. Platonov, V. Nikulina, Y. Kucheryavyi, V. Gryshchuk, Y. Stohniy, V. Chernyshenko, O. Slominskyi, A. Rebriev, K. Savchenko, L. Garmanchuk","doi":"10.15407/ubj94.05.007","DOIUrl":"https://doi.org/10.15407/ubj94.05.007","url":null,"abstract":"Disintegrins are the antagonists of integrin receptors that can be found mostly in snakes’ venom. They can inhibit platelet aggregation, thus preventing the formation of blood clots. By blocking the integrin receptors of cancer cells, disintegrins can inhibit proliferation and metastasis. Thus, the search for new sources of disintegrins and development of methods of their purification is an important task of modern biotechnology. This work was dedicated to the purification and characterization of inhibiting polypeptides from Bitis arietans venom. Crude venom of B. arietans was fractionated using ion-exchange chromatography on Q Sepharose followed by size-exclusion chromatography on Superdex 75 using FPLC method. Analysis of molecular weight of protein components was performed using SDS-PAGE and MALDI-TOF analysis on Voyager-DE. Aggregation of platelet-rich plasma (PRP) in the presence of platelet aggregation inhibitor was investigated using aggregometry on the AR2110. MTT test was used for measuring HeLa cells proliferation and survival in vitro. Two-step chromatography allowed us to obtain fraction that contained polypeptides possessing the dose-dependent inhibitory action on adenosine diphosphate (ADP)-induced platelet aggregation in PRP. SDS-PAGE showed that obtained fraction contained two polypeptides with molecular weight 9.0 and 13.67 kDa according to MALDI-TOF analysis. Purified polypeptides inhibited ADP-induced platelet aggregation with IC50 0.09 mg/ml. However, 0.005 mg/ml of fraction suppressed viability of HeLa cells according to MTT test on 20%. Discovered biological effects of fractions allowed us to conclude the possible use of these polypeptides as anti-aggregatory or anti-proliferative agents. Keywords: antithrombotic action, disintegrins, glycoprotein IIb/IIIa, platelets, snake venom","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72752677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution and polymorphism of enzymes involved in antioxidant protection and xenobiotics biotransformation in the mediterranean mussel Mytilus galloprovincialis 地中海贻贝(Mytilus galloprovincialis)抗氧化保护和外源生物转化酶的分布与多态性

The Ukrainian Biochemical Journal Pub Date : 2022-11-10 DOI: 10.15407/ubj94.04.067

V. Toptikov

引用次数: 0

Fatty acid profile of the liver lipids under acute fungicide action and intake of a biologically active preparation in rats 急性杀菌剂作用下大鼠肝脏脂质脂肪酸谱及生物活性制剂的摄入

The Ukrainian Biochemical Journal Pub Date : 2022-11-10 DOI: 10.15407/ubj94.04.047

S. Khyzhnyak, S. Midyk, A. O. Velinska, O. Arnauta, L. Н. Kalachniuk

引用次数: 0

Structural proteins in the mechanisms of betacoronavirus virion assembly 冠状病毒病毒粒子组装机制中的结构蛋白

The Ukrainian Biochemical Journal Pub Date : 2022-11-10 DOI: 10.15407/ubj94.04.005

I. Zaloilo, О. Zaloilo, Y. Rud, L. Buchatskyi

引用次数: 1

Studies of the molecular basis of eukaryotic transcription. Roger Kornberg the Nobel prize in Chemistry 2006 真核生物转录的分子基础研究。罗杰·科恩伯格获2006年诺贝尔化学奖

The Ukrainian Biochemical Journal Pub Date : 2022-11-10 DOI: 10.15407/ubj94.04.093

O. P. Matyshvska

引用次数: 0

Assessing the in vitro anti-glycation efficacy of vitamins A, C, D, E 评价维生素A、C、D、E的体外抗糖化作用

The Ukrainian Biochemical Journal Pub Date : 2022-11-10 DOI: 10.15407/ubj94.04.061

N. P. Dalbanjan, A. J. Kadapure, P. Huded, V. Chachadi, S. Nayaka, Praveen Kumar S.K.

引用次数: 0

Localization and level of proapoptotic protein regulators in a rat lung tissue during development of acute experimental bronchopneumonia 急性实验性支气管肺炎大鼠肺组织中促凋亡蛋白调节因子的定位和水平

The Ukrainian Biochemical Journal Pub Date : 2022-11-10 DOI: 10.15407/ubj94.04.036

D. Ziablitsev, A. Tykhomyrov, O. Dyadyk, S. Kolesnikova, S. Ziablitsev

{"title":"Localization and level of proapoptotic protein regulators in a rat lung tissue during development of acute experimental bronchopneumonia","authors":"D. Ziablitsev, A. Tykhomyrov, O. Dyadyk, S. Kolesnikova, S. Ziablitsev","doi":"10.15407/ubj94.04.036","DOIUrl":"https://doi.org/10.15407/ubj94.04.036","url":null,"abstract":"Apoptosis plays an important role in the development of acute inflammatory lung injury (AILI) and its consequences, which can be realized in different cells with distinct intensity and rate. The aim of this study was to determine the distribution and expression intensity of apoptosis markers in the lungs of rats in the AILI model with endotracheal introduction of capron thread and LPS. Immunoblotting and immunohistochemical studies were performed using monoclonal antibodies against Bax and caspase-3 proteins. It was shown that Bax level increased significantly with the peak on the 7th day. The second peak of Bax 40 dimeric form was noted on the 21st day. The level of both pro-caspase-3 and active caspase-3 was also dramatically increased with a maximum on the 5th day and the second peak of active caspase-3 content was observed on the 21st day. These changes reflected the activation of apoptosis in key trigger periods of AILI during the development of exudative hemorrhagic pneumonia and subsequent fibrotic remodeling of the lungs. Keywords: AILI, apoptosis, Bax, caspase-3, lipopolysaccharide","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75487581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Black tea and coffee impact on steroid hormones status in young men 红茶和咖啡对年轻男性类固醇激素水平的影响

The Ukrainian Biochemical Journal Pub Date : 2022-11-10 DOI: 10.15407/ubj94.04.083

M. F. Amirova, A. Dadashova, E. Huseynova, I. Kerimova, S. I. Hasanova, F. E. Guliyeva, S. R. Guliyeva, R. Rahimova, G. Vahabova

{"title":"Black tea and coffee impact on steroid hormones status in young men","authors":"M. F. Amirova, A. Dadashova, E. Huseynova, I. Kerimova, S. I. Hasanova, F. E. Guliyeva, S. R. Guliyeva, R. Rahimova, G. Vahabova","doi":"10.15407/ubj94.04.083","DOIUrl":"https://doi.org/10.15407/ubj94.04.083","url":null,"abstract":"Tea and coffee alkaloids affect the hormonal status of the body. There are reports about the effect of caffeine on the body under stress, but nearly absent reports on the effect of tea and coffee alkaloids at rest. The aim of this work was to determine whether there is a significant difference in testosterone and cortisol concentrations in the blood of young men before and after drinking indicated beverages. The work was carried out on 21 healthy young males that were tested for blood cortisol levels before and after drinking tea or coffee on an empty stomach. The young men were divided into two groups: the first group comprised those whose cortisol levels increased after taking a single dose of tea, the second group – whose cortisol decreased. The third group comprised persons who took a single dose of strong-grain coffee. In addition, adrenaline and testosterone levels were determined as a hormonal panel. Our pioneer investigation found that coffee causes a significant decrease in cortisol levels at rest, but tea consumption by coffee drinkers leads to a more pronounced decrease in the cortisol levels than coffee. Keywords: black tea, coffee, cortisol, young man","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85696517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Lipid parameters in the blood of coronary artery diseases patients depending on age and dietary components: a case study 冠状动脉疾病患者血液中的脂质参数与年龄和饮食成分的关系:一个案例研究

The Ukrainian Biochemical Journal Pub Date : 2022-11-10 DOI: 10.15407/ubj94.04.054

S. Prasad

引用次数: 0

Inhibition of plasma membrane Сa(2+),Mg(2+)-АТРase by сalixarene sulfonylamidines. Structure-activity relationship 链芳烃磺酰基胺对质膜Сa(2+)，Mg(2+)-АТРase的抑制作用。结构与活性关系

The Ukrainian Biochemical Journal Pub Date : 2022-11-10 DOI: 10.15407/ubj94.04.018

O. A. Shkrabak, T. O. Veklich, R. Rodik, V. Kalchenko, S. Kosterin

{"title":"Inhibition of plasma membrane Сa(2+),Mg(2+)-АТРase by сalixarene sulfonylamidines. Structure-activity relationship","authors":"O. A. Shkrabak, T. O. Veklich, R. Rodik, V. Kalchenko, S. Kosterin","doi":"10.15407/ubj94.04.018","DOIUrl":"https://doi.org/10.15407/ubj94.04.018","url":null,"abstract":"Previously we have already shown that tetrasulfonylamidinecalixarene C-90 inhibited plasma membrane Са2+,Mg2+-АТРаse of smooth muscle cells selectively to other ATPases of plasma membrane. To inhance the inhibitory effect of calixarenes several alkoxycalixarene sulfonylamidines structurally similar to calixarene C-90 were synthesized and their effects on the mentioned enzyme activity, the level of cytoplasmic Ca2+ concentration and hydrodynamic diameter of isolated smooth muscle cells were checked. It was shown that sulfonylamidino groups are crucial for Са2+,Mg2+-АТРаse inhibition, the efficiency of inhibition depends on their quantity and spatial orientation at the upper rim of calixarene macrocycle. Introduction of phenyl or tert-butyl groups into the upper rim and of long alkyl chains into the lower rim led to only slightl increase of inhibition efficiency. The inhibitory effect of studied calixarenes on Са2+,Mg2+-АТРаse correlated with effects on cytosolic Ca2+ concentration and hydrodynamic diameter of smooth muscle cells. The obtained results are important for creation of more effective and selective inhibitors of plasma membrane Са2+,Mg2+-АТРаse as regulators of smooth muscle contractility. Keywords: calixarene sulfonylamidines, intracellular Ca(2+) concentration, plasma membrane Са(2+);Mg(2+)-АТРаse, smooth muscle","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86345254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1