Localization and level of proapoptotic protein regulators in a rat lung tissue during development of acute experimental bronchopneumonia

The Ukrainian Biochemical Journal Pub Date : 2022-11-10 DOI:10.15407/ubj94.04.036

D. Ziablitsev, A. Tykhomyrov, O. Dyadyk, S. Kolesnikova, S. Ziablitsev

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引用次数: 2

Abstract

Apoptosis plays an important role in the development of acute inflammatory lung injury (AILI) and its consequences, which can be realized in different cells with distinct intensity and rate. The aim of this study was to determine the distribution and expression intensity of apoptosis markers in the lungs of rats in the AILI model with endotracheal introduction of capron thread and LPS. Immunoblotting and immunohistochemical studies were performed using monoclonal antibodies against Bax and caspase-3 proteins. It was shown that Bax level increased significantly with the peak on the 7th day. The second peak of Bax 40 dimeric form was noted on the 21st day. The level of both pro-caspase-3 and active caspase-3 was also dramatically increased with a maximum on the 5th day and the second peak of active caspase-3 content was observed on the 21st day. These changes reflected the activation of apoptosis in key trigger periods of AILI during the development of exudative hemorrhagic pneumonia and subsequent fibrotic remodeling of the lungs. Keywords: AILI, apoptosis, Bax, caspase-3, lipopolysaccharide

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急性实验性支气管肺炎大鼠肺组织中促凋亡蛋白调节因子的定位和水平

细胞凋亡在急性炎症性肺损伤(acute inflammatory lung injury, AILI)及其后果的发生发展中起着重要作用，其发生在不同的细胞中，且发生的强度和速率不同。本研究的目的是测定经气管内注入capron线和LPS的AILI模型大鼠肺中凋亡标志物的分布和表达强度。使用抗Bax和caspase-3蛋白的单克隆抗体进行免疫印迹和免疫组织化学研究。结果表明，Bax水平显著升高，在第7天达到峰值。bax40二聚体的第二峰出现在第21天。前caspase-3和活性caspase-3含量也显著增加，在第5天达到最大值，活性caspase-3含量在第21天出现第二个高峰。这些变化反映了在渗出性出血性肺炎发展和随后肺纤维化重塑过程中，aii关键触发期细胞凋亡的激活。关键词:AILI，凋亡，Bax, caspase-3，脂多糖

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The Ukrainian Biochemical Journal

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