The Ukrainian Biochemical Journal最新文献_第10页

Comparison of adjuvant properties of chitosan during oral and subcutaneous immunization of mice with BSA 壳聚糖对小鼠BSA口服和皮下免疫佐剂性能的比较

The Ukrainian Biochemical Journal Pub Date : 2022-07-11 DOI: 10.15407/ubj94.02.031

M. Kozak, I. Petruh, V. Vlizlo

{"title":"Comparison of adjuvant properties of chitosan during oral and subcutaneous immunization of mice with BSA","authors":"M. Kozak, I. Petruh, V. Vlizlo","doi":"10.15407/ubj94.02.031","DOIUrl":"https://doi.org/10.15407/ubj94.02.031","url":null,"abstract":"Vaccination is the best method to prevent the spread of infectious diseases, its disadvantages are side effects. Potentially safe DNA, RNA or protein molecules possess antigenic properties, but are low-immunogenic and therefore require conjugation with an adjuvant. The aim of the research was to evaluate Chitosan (CS) potency as an adjuvant and compare its effectiveness depending on the route of drug administration. The experiments were carried out on 3 groups of BALB/c mice. Mice of the first group were injected subcutaneously with 20 µl of a mixture of CS (3.3 mg/kg) and BSA (1.7 mg/kg). The mixture of CS and BSA at the same doses and volume was administered orally to mice of the second experimental group. The third group – control – unvaccinated mice. Anti-BSA antibody levels were measured by ELISA. Aspartate aminotransferase, alanine aminotransferase activity and cholesterol, creatinine and urea levels were determined in the serum. It was found that both subcutaneous and mucosal immunizations provided a 2-fold increase in anti-BSA antibody titers against the background of maintaining all biochemical blood parameters at the level of the physiological norm. However, AST activity in the serum of oral-immunized mice was elevated as compared to subcutaneous-immunized mice. Serum cholesterol level in the group of subcutaneously immunized mice and creatinine and urea levels in both experimental groups were reduced compared to the control. It is concluded that oral immunization with CS is the optimal route for antigen-specific IgG antibody response induction.","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73331967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Changes in the expression of TRPV4 and TRPM8 channels in the colon of rats with 6-OHDA-induced Parkinson’s disease 6-羟多巴胺诱导帕金森病大鼠结肠中TRPV4和TRPM8通道表达的变化

The Ukrainian Biochemical Journal Pub Date : 2022-07-11 DOI: 10.15407/ubj94.02.057

V. Stetska, T. Dovbynchuk, N. Dziubenko, A. Zholos, G. Tolstanova

{"title":"Changes in the expression of TRPV4 and TRPM8 channels in the colon of rats with 6-OHDA-induced Parkinson’s disease","authors":"V. Stetska, T. Dovbynchuk, N. Dziubenko, A. Zholos, G. Tolstanova","doi":"10.15407/ubj94.02.057","DOIUrl":"https://doi.org/10.15407/ubj94.02.057","url":null,"abstract":"Parkinson’s disease (PD) is neurodegenerative disease, which is accompanied by degeneration of dopaminergic neurons in subtantia nigra. Non-motor symptoms, in particular, disorders of the gastrointestinal (GI) tract are observed in 20-80% of patients some 15-20 years before clinically diagnosed PD and are not a least important feature of PD pathogenesis. The transient receptor potential (TRP) channels are expressed throughout the GI tract, where they play an important role in taste, thermoregulation, pain, mucosal function and homeostasis, control of interstitial motility etc. The aim of this study was to investigate the contribution of TRPV4 and TRPM8 channels in the GI motor function in the colon of rats with PD, incduced by injection of the 12 μg 6-hydroxydopamine (6-OHDA). The studies were performed on the 4th week and the 7th month after PD induction The rats were randomly divided into: I group – the sham-lesioned rats, 4 μl 0.9% NaCl, autopsy 4 weeks after injection (n = 5); II group – the 6-OHDA-PD rats, 4 μl 12 μg of 6-OHDA, autopsy 4 weeks after injection (n = 5); III group – the sham-lesioned rats, 4 μl 0.9% NaCl, autopsy 7 months after injection (n = 4); IV group – the 6-OHDA-PD rats, 4 μl 12 μg of 6-OHDA, autopsy 7 months after injection (n = 5). We evaluated the body weight of rats, GI transit time, the cecum weight index and immunohistochemical identification of tyrosine hydroxylase (TH) -positive cells, and TRPV4, TRPM8 expression in rat’s colon. We showed that on the 7th month of the experiment, the GI transit time doubles over time; the cecum weight index of 6-OHDA rats increased by 57%; the number of TH-positive cells in colon rats decreased 2-fold, while TRPM8 ion channels were downregulated in PD rats and TRPV4 ion channels were upregulated in the colon of rats with 6-OHDA-PD. It was concluded that TRPV4 and TRPM8 ion channels may be considered pharmacological targets in the progression of PD pathology.","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87071270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Thrombomodulin and von willebrand factor as markers of endothelial dysfunction in patients with chronic kidney disease 血栓调节蛋白和血管性血友病因子作为慢性肾病患者内皮功能障碍的标志物

The Ukrainian Biochemical Journal Pub Date : 2022-07-11 DOI: 10.15407/ubj94.02.045

I. Mykhaloiko, R. Yatsyshyn, N. Cherniuk, M. Humeniuk

{"title":"Thrombomodulin and von willebrand factor as markers of endothelial dysfunction in patients with chronic kidney disease","authors":"I. Mykhaloiko, R. Yatsyshyn, N. Cherniuk, M. Humeniuk","doi":"10.15407/ubj94.02.045","DOIUrl":"https://doi.org/10.15407/ubj94.02.045","url":null,"abstract":"The aim of research was to study the levels of thrombomodulin (TM) and von Willebrand factor (VWF) in the serum and urine of patients with chronic kidney disease (CKD)as diagnostic markers of endothelial dysfunction. The study involved 140 patients with CKD. The clinical diagnosis was determined based on standard methods of patients examination according to the kidney diseases classification and protocols of CKD patients management. The concentrations of TM and VWF in serum and urine were quantified by ELISA. A generalized endothelial dysfunction in the vessels of the whole body, including the kidneys and high concentration of TM and FVF in the serum and urine of patients with a diabetic nephropathy have been found. The concentration of TM and VWF in the serum of patients with a chronic glomerulonephritis was at the same level as in the serum of healthy individuals, while those in urine significantly exceeded the control values, indicating endothelial damage in the glomeruli of the kidneys due to exposure to pro-inflammatory cytokines. In our opinion, the studied markers will contribute to the timely diagnosis of endothelial dysfunction in patients with CKD and to the development of criteria for prescribing antiplatelet agents in glomerular kidney disease.","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76558900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Heat shock proteins in adaptation to physical activity 适应身体活动的热休克蛋白

The Ukrainian Biochemical Journal Pub Date : 2022-07-11 DOI: 10.15407/ubj94.02.005

V. Kuibida, P. Kokhanets, V. Lopatynska

引用次数: 1

Application of gold nanoparticles to determine spermine in the presence of other polyamines 应用金纳米粒子测定存在其他多胺的精胺

The Ukrainian Biochemical Journal Pub Date : 2022-07-11 DOI: 10.15407/ubj94.02.066

Y. Yanish, M. Prylutskyi, S. Zaletok, Y. Mukha

引用次数: 0

Study of matrix metalloproteinase activity in patients with autoimmune thyroiditis 自身免疫性甲状腺炎患者基质金属蛋白酶活性的研究

The Ukrainian Biochemical Journal Pub Date : 2022-07-11 DOI: 10.15407/ubj94.02.051

R. Rahimova

{"title":"Study of matrix metalloproteinase activity in patients with autoimmune thyroiditis","authors":"R. Rahimova","doi":"10.15407/ubj94.02.051","DOIUrl":"https://doi.org/10.15407/ubj94.02.051","url":null,"abstract":"One of the most important pathogenetic mechanisms of autoimmune thyroiditis (AIT) is the violation of immunological tolerance and the development of the autoimmune process, the markers of which are various biologically active substances, in particular, matrix metalloproteinases (MMP) of the extracellular matrix (ECM). MMPs play a crucial role in the development of pathological processes in these diseases, contributing to matrix degradation due to imbalance between the activity of enzymes and their inhibitors. The aim of the work was to study the activity of key metalloproteinases and the level of α2-macroglobulin in patients with autoimmune thyroiditis. The diagnosis of AIT was established based on the study of data on anamnesis, thyroid status, the results of ultrasound of TG, and the presence of antibodies to the thyroid-stimulating hormone receptor (TSH) in blood plasma. Patients were enrolled in 2 groups: group 1 – 74 patients with a manifest form of the disease; group 2 – 96 patients with a subclinical form of the disease. The study of matrix metalloprotein activity in the examined patients showed a statistically significant (P = 0.015) increase in MMP-3 and MMP-7 activity in patients with AIT compared to the corresponding parameters in persons of the control group. Thus, levels of MMP-3 and 7 were in the group of patients, respectively 56 (51.0; 59.0) and 4.6 (4.3; 5.2) ng/ml, in control 23.0 (16.0; 26.0) and 3.6 (3.4; 4.1) ng/ml, respectively.","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77836295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticles application as a therapeutic strategy for diabetes mellitus management 纳米颗粒在糖尿病治疗中的应用

The Ukrainian Biochemical Journal Pub Date : 2022-07-11 DOI: 10.15407/ubj94.02.015

A. Ojo, A. I. Oni, D. Rotimi, M. Iyobhebhe, P. O. Adeniji, J. Talabi, O. A. Ojo

引用次数: 0

Unraveling the mystery of nitric oxide: Nobel prize winners Robert Furchgott, Louis Ignarro, and Ferid Murad 揭开一氧化氮的神秘面纱:诺贝尔奖得主罗伯特·弗奇戈特、路易斯·伊格纳罗和费里德·穆拉德

The Ukrainian Biochemical Journal Pub Date : 2022-07-11 DOI: 10.15407/ubj94.02.085

T. Danylova, S. Komisarenko

引用次数: 0

Calix[4]arene chalcone amide C-1011 elicits differential effects on the viability of 4T1 mouse breast adenocarcinoma cells with different levels of adaptor protein Ruk/CIN85 expression 杯[4]芳烃查尔酮酰胺C-1011对适配蛋白Ruk/CIN85表达水平不同的4T1小鼠乳腺腺癌细胞的生存能力有不同的影响

The Ukrainian Biochemical Journal Pub Date : 2022-07-11 DOI: 10.15407/ubj94.02.024

L. Babich, S. Shlykov, O. Yesypenko, A. O. Bavelska-Somak, A. G. Zahoruiko, I. Horak, L. Drobot, S. Kosterin

{"title":"Calix[4]arene chalcone amide C-1011 elicits differential effects on the viability of 4T1 mouse breast adenocarcinoma cells with different levels of adaptor protein Ruk/CIN85 expression","authors":"L. Babich, S. Shlykov, O. Yesypenko, A. O. Bavelska-Somak, A. G. Zahoruiko, I. Horak, L. Drobot, S. Kosterin","doi":"10.15407/ubj94.02.024","DOIUrl":"https://doi.org/10.15407/ubj94.02.024","url":null,"abstract":"According to our earlier data, calix[4]arene chalcone amides modulate Ca ions exchange in the myometrium mitochondria and the level of inner membrane polarization that can potentially affect cell survival. To test this hypothesis, we studied the effect of calix[4]arene with 4 chalcone amide groups on mitochondria membrane polarization and viability of 4T1 mouse breast adenocarcinoma cells, a surrogate model of human triple-negative breast cancer, and on its highly malignant subline overexpressing the adaptor protein Ruk/CIN85. Mitochondria membrane potential was measured by flow cytometry, and cell viability was assessed using Trypan blue dye exclusion. It was shown that mitochondrial membranes of control (Mock) cells had a higher polarization level (67.80 ± 8.82 r.u., n = 5) compared to 4T1 cells with up-regulation of Ruk/CIN85 (RukUp cells) (25.42 ± 2.58 r.u., n = 4). Upon incubation of cells with 1 μM calix[4]arene C-1011, the CCCP-sensitive component of mitochondrial membranes polarization decreased (by almost 50%) in 4T1 Mock cells and did not change in RukUp cells compared with the control. It was demonstrated that 1 μM calix[4]arene C-1011 suppressed the viability of 4T1 Mock cells by 45%, but did not affect RukUp cells considerably. It was suggested that calix[4]arene chalcone amide С-1011 decreased mouse breast adenocarcinoma 4T1 cell viability at least by affecting mitochondrial membrane polarization.The data obtained indicate the prospects of further studies of calix[4]arene chalcone amide as a potential anticancer drug candidate.","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81785488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of chitosan molecular weight profile by electrophoresis in a porosity step gradient polyacrylamide gel 孔隙阶跃梯度聚丙烯酰胺凝胶电泳分析壳聚糖分子量谱

The Ukrainian Biochemical Journal Pub Date : 2022-07-11 DOI: 10.15407/ubj94.02.076

M. Lootsik, N. Manko, R. Bilyy, M. Lutsyk (Jr.), R. Stoika

{"title":"Analysis of chitosan molecular weight profile by electrophoresis in a porosity step gradient polyacrylamide gel","authors":"M. Lootsik, N. Manko, R. Bilyy, M. Lutsyk (Jr.), R. Stoika","doi":"10.15407/ubj94.02.076","DOIUrl":"https://doi.org/10.15407/ubj94.02.076","url":null,"abstract":"Chitosan is biocompatible and biodegradable natural biopolymer widely applied in various fields of biology, medicine, and pharmacy, however, its effects significantly depend on the degree of polymerization (DP) and the degree of deacetylation (DDA) of polymer chains. Evaluation of the chitosan chain diversity by DP requires the use of a highly expensive method of high-performance size exclusion chromatography. The aim of our study was to determine the molecular weight profile of chitosan specimens by the use of electrophoresis in a porosity step gradient polyacrylamide gel and to evaluate the efficacy of this method in monitoring the purification of chitosan fragments and its derivatives. Two types of step gradient porosity gels were used: 1) gels of layers with acrylamide concentration 2.5, 3.5, 5.0, 10.0, 15.0, 20.0 % w/v for native chitosan or its high molecular fragments; 2) gels of layers with acrylamide concentration 2.5, 5.0, 10.0, 15.0, 20.0, 25.0 % w/v for low molecular chitosan fragments. The main amount of molecules from the chitosan pool was localized in the type 1 gel in the region of 550-40 kDa and distributed among three bands, which in different samples differed significantly in percentage. Electrophoresis of chitosan fragments fractionated by gel permeation chromatography provided a clear separation of medium molecular weight fragments (50–400 kDa) in type 1 gel and of low molecular weight fragments (3–40 kDa) in type 2 gel. Thus the method of chitosan electrophoresis in a step-gradient porosity of polyacrylamide gel was developed which permits to characterize the molecular weight profile of chitosan specimens polymer chains and is effective in monitoring the isolation of chitosan fragments by gel penetration chromatography of molecular weights from 3 to 400 kDa.","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90640058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0