壳聚糖对小鼠BSA口服和皮下免疫佐剂性能的比较

M. Kozak, I. Petruh, V. Vlizlo
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引用次数: 1

摘要

接种疫苗是预防传染病传播的最好方法,它的缺点是副作用。潜在安全的DNA、RNA或蛋白质分子具有抗原性,但免疫原性低,因此需要与佐剂结合。本研究的目的是评价壳聚糖(CS)作为佐剂的效力,并比较其在不同给药途径下的效果。实验在3组BALB/c小鼠上进行。第一组小鼠皮下注射CS (3.3 mg/kg)和BSA (1.7 mg/kg)混合物20µl。第二实验组小鼠口服相同剂量和体积的CS和BSA混合物。第三组是未接种疫苗的对照组小鼠。ELISA法检测血清抗bsa抗体水平。测定血清中谷草转氨酶、丙氨酸转氨酶活性及胆固醇、肌酐、尿素水平。研究发现,皮下和粘膜免疫均可使抗bsa抗体滴度增加2倍,同时维持所有血液生化参数在生理标准水平。然而,与皮下免疫小鼠相比,口服免疫小鼠血清中AST活性升高。皮下免疫组小鼠血清胆固醇水平和两组小鼠肌酐、尿素水平均较对照组降低。综上所述,口服免疫CS是诱导抗原特异性IgG抗体应答的最佳途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of adjuvant properties of chitosan during oral and subcutaneous immunization of mice with BSA
Vaccination is the best method to prevent the spread of infectious diseases, its disadvantages are side effects. Potentially safe DNA, RNA or protein molecules possess antigenic properties, but are low-immunogenic and therefore require conjugation with an adjuvant. The aim of the research was to evaluate Chitosan (CS) potency as an adjuvant and compare its effectiveness depending on the route of drug administration. The experiments were carried out on 3 groups of BALB/c mice. Mice of the first group were injected subcutaneously with 20 µl of a mixture of CS (3.3 mg/kg) and BSA (1.7 mg/kg). The mixture of CS and BSA at the same doses and volume was administered orally to mice of the second experimental group. The third group – control – unvaccinated mice. Anti-BSA antibody levels were measured by ELISA. Aspartate aminotransferase, alanine aminotransferase activity and cholesterol, creatinine and urea levels were determined in the serum. It was found that both subcutaneous and mucosal immunizations provided a 2-fold increase in anti-BSA antibody titers against the background of maintaining all biochemical blood parameters at the level of the physiological norm. However, AST activity in the serum of oral-immunized mice was elevated as compared to subcutaneous-immunized mice. Serum cholesterol level in the group of subcutaneously immunized mice and creatinine and urea levels in both experimental groups were reduced compared to the control. It is concluded that oral immunization with CS is the optimal route for antigen-specific IgG antibody response induction.
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