ACS Omega最新文献_第6页

A Versatile Fabrication Route for Screening of Block Copolymer Membranes in Bioprocessing

IF 3.7 3区化学

ACS Omega Pub Date : 2025-02-20 DOI: 10.1021/acsomega.4c1126910.1021/acsomega.4c11269

Ke Meng, Alberto Alvarez-Fernandez, Stefan Guldin* and Daniel G. Bracewell*,

{"title":"A Versatile Fabrication Route for Screening of Block Copolymer Membranes in Bioprocessing","authors":"Ke Meng, Alberto Alvarez-Fernandez, Stefan Guldin* and Daniel G. Bracewell*, ","doi":"10.1021/acsomega.4c1126910.1021/acsomega.4c11269","DOIUrl":"https://doi.org/10.1021/acsomega.4c11269https://doi.org/10.1021/acsomega.4c11269","url":null,"abstract":"Traditional poly(ether sulfone) (PES) filters, widely used for sterile, viral, and ultrafiltration, often exhibit restrictions in their selectivity-permeability profile due to their heterogeneous pore size distribution. This limitation has sparked interest in developing novel isoporous membrane materials and fabrication techniques. Among promising candidates, block copolymer (BCP) membranes produced via self-assembly and nonsolvent-induced phase separation (SNIPS) offer significant advantages, including tunable pore size, narrow pore size distribution, high porosity, and enhanced mechanical flexibility. However, optimizing the structure formation in SNIPS remains a complex and time-consuming process, making it unsuitable for rapidly screening new BCP candidates. In response, this study introduces an alternative fabrication approach based on the direct spin-coating of BCPs onto anodic aluminum oxide (AAO) supports. Using this method, a poly(styrene)-block-poly(methyl methacrylate) (PS-b-PMMA) thin film was directly cast onto a water-filled AAO support, enabling the formation of an isoporous membrane structure for filtration applications, significantly reducing the complexity of structure–application optimization. When compared to commercial PES membranes with similar molecular weight cut-offs, these novel PS-b-PMMA thin-film composite membranes exhibited comparable transmission rates for bovine serum albumin and a monoclonal antibody, while delivering a ninefold improvement for thyroglobulin rejection. This superior cutoff precession highlights their potential to remove viruses and antibody aggregates during the downstream processing of monoclonal antibody production. By reducing the burden of chromatographic polishing steps, this advance offers promise for enhancing efficiency and lowering costs in biopharmaceutical manufacturing.","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 8","pages":"8630–8639 8630–8639"},"PeriodicalIF":3.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c11269","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental Study on Gas Flow Characteristics in Coal under Different Mining-Induced Stress Paths

IF 3.7 3区化学

ACS Omega Pub Date : 2025-02-19 DOI: 10.1021/acsomega.4c1029410.1021/acsomega.4c10294

Xinglin Qin, Linfan Qi, Xianfeng Liu*, Yankun Ma*, Yaolin Cao and Baisheng Nie,

{"title":"Experimental Study on Gas Flow Characteristics in Coal under Different Mining-Induced Stress Paths","authors":"Xinglin Qin, Linfan Qi, Xianfeng Liu*, Yankun Ma*, Yaolin Cao and Baisheng Nie, ","doi":"10.1021/acsomega.4c1029410.1021/acsomega.4c10294","DOIUrl":"https://doi.org/10.1021/acsomega.4c10294https://doi.org/10.1021/acsomega.4c10294","url":null,"abstract":"To investigate the impact of stress variations on coal permeability during mining, this study combines theoretical analysis with experimental research to reveal the permeability variation patterns under three mining-induced stress conditions. The results indicate that coal permeability exhibits a three-stage variation characteristic under different mining-induced stresses: slow increase, significant increase, and rapid increase. As axial stress increases, permeability initially decreases and then stabilizes. After reaching the strain peak and coal sample failure, through-cracks form on the sample surface, leading to a significant permeability increase. During the quasi-hydrostatic pressure loading stage, permeability decreases with increasing stress, while it gradually recovers during the confining pressure unloading stage, with a notable increase after reaching peak stress. Gas extraction efficiency is particularly ideal after the stress peak, with the most significant effect observed in the pressure relief zone. Based on the Forchheimer equation and the Klinkenberg effect, the calculated results indicate a parabolic variation trend between gas flow rate and pressure gradient, as well as between pore pressure and permeability.","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 8","pages":"8388–8397 8388–8397"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c10294","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan Schiff-Base Hydrogel Sunscreen: A Multifunctional Hybrid Network with Antioxidant, Ultraviolet-Shielding, and Self-Healing Properties

IF 3.7 3区化学

ACS Omega Pub Date : 2025-02-19 DOI: 10.1021/acsomega.4c0997610.1021/acsomega.4c09976

Nafiseh Heydari, Ali Reza Karimi*, Hamid Reza Momeni, Farnaz Azadikhah and Tahereh Etemadi,

{"title":"Chitosan Schiff-Base Hydrogel Sunscreen: A Multifunctional Hybrid Network with Antioxidant, Ultraviolet-Shielding, and Self-Healing Properties","authors":"Nafiseh Heydari, Ali Reza Karimi*, Hamid Reza Momeni, Farnaz Azadikhah and Tahereh Etemadi, ","doi":"10.1021/acsomega.4c0997610.1021/acsomega.4c09976","DOIUrl":"https://doi.org/10.1021/acsomega.4c09976https://doi.org/10.1021/acsomega.4c09976","url":null,"abstract":"Prolonged exposure to ultraviolet (UV) radiation can cause erythema, sunburn, inflammation, and even skin cancer. Sunscreen is highly effective in protecting against UV radiation. Hydrogels, due to their similarity to the skin’s extracellular matrix, flexibility, and high water content, have been widely used for sunscreen applications. However, traditional sunscreens may have limitations, including skin irritation, environmental impact, and formulation issues. This study introduces two innovative chitosan-based hydrogel sunscreens integrating 2,4-dihydroxybenzaldehyde (DHBA) and vanillin (VA) as UV absorbers and cross-linkers. The synthesized CS/DHBA and CS/DHBA/VA hydrogels, leveraging a hybrid network of Schiff-base and intermolecular hydrogen bonds, demonstrated exceptional UV-shielding capabilities with broad absorption of UVB and UVA regions (290–400 nm). Furthermore, the hydrogels exhibited a porous three-dimensional interconnected microstructure with self-healing and potent antioxidant properties. In vitro sun protection factor values and in vivo experiments confirmed the efficacy of the hydrogel sunscreens for robust UV protection. Importantly, in vivo skin toxicity assessments established the safety of the hydrogels for skin application. The multifunctional nature of the chitosan-based hydrogel sunscreens, coupled with UV-shielding properties, underscores their potential as a valuable biomaterial for skin protection. These findings show great promise for improving sunscreen technology and offer a pathway for developing next-generation UV-protective skincare products.","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 8","pages":"8250–8261 8250–8261"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c09976","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

XGBMUT: Predicting the Functional Impact of Missense Mutations Using an Extreme Gradient Boost Classifier

IF 3.7 3区化学

ACS Omega Pub Date : 2025-02-19 DOI: 10.1021/acsomega.4c1017910.1021/acsomega.4c10179

Gabriel Rodrigues Coutinho Pereira*, Loiane Mendonça Abrantes Da Conceição, Bárbara de Azevedo Abrahim-Vieira, Carlos Rangel Rodrigues, Lucio Mendes Cabral, Ricardo Limongi França Coelho and Joelma Freire De Mesquita,

{"title":"XGBMUT: Predicting the Functional Impact of Missense Mutations Using an Extreme Gradient Boost Classifier","authors":"Gabriel Rodrigues Coutinho Pereira*, Loiane Mendonça Abrantes Da Conceição, Bárbara de Azevedo Abrahim-Vieira, Carlos Rangel Rodrigues, Lucio Mendes Cabral, Ricardo Limongi França Coelho and Joelma Freire De Mesquita, ","doi":"10.1021/acsomega.4c1017910.1021/acsomega.4c10179","DOIUrl":"https://doi.org/10.1021/acsomega.4c10179https://doi.org/10.1021/acsomega.4c10179","url":null,"abstract":"Millions of new mutations have been discovered largely due to advancements in genome projects, but characterizing their effects through traditional wet-lab experiments remains labor-intensive and time-consuming. Functional prediction algorithms offer a solution by enabling the efficient screening of mutations, thereby saving time and resources. The objective of this study was to develop a competitive algorithm for predicting the functional impact of missense mutations. A unified database and substitution matrices containing predictor variables specifically for missense mutations were initially constructed. Subsequently, values for the predictor variables were collected from the training and test sets derived from the ClinVar and HumsaVar databases. A series of supervised machine learning classifiers were then trained, and their performance was evaluated using the test set. The best-performing model was additionally compared against ten currently available functional prediction algorithms. The proposed algorithm, XGBMut, demonstrates exceptional accuracy in classifying missense mutations while also exhibiting a competitive performance. Additionally, a user-friendly graphical interface was developed to enhance accessibility for professionals in various fields. Unlike most existing methods, XGBMut eliminates the need for a web server dependency and the installation of third-party software, making it a more versatile tool for users.","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 8","pages":"8349–8360 8349–8360"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c10179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Mobility, High-Photostability Tetravalent-Terbium-Doped Indium–Tin–Zinc-Oxide Thin-Film Transistors with a Stacked-Channel Structure

IF 3.7 3区化学

ACS Omega Pub Date : 2025-02-19 DOI: 10.1021/acsomega.4c0999410.1021/acsomega.4c09994

Huimin Su, Linfeng Lan*, Jintao Xu, Qi Zhou, Yaping Li, Dechun Zeng, Shuai Yang, Baozhong Chen and Junbiao Peng,

{"title":"High-Mobility, High-Photostability Tetravalent-Terbium-Doped Indium–Tin–Zinc-Oxide Thin-Film Transistors with a Stacked-Channel Structure","authors":"Huimin Su, Linfeng Lan*, Jintao Xu, Qi Zhou, Yaping Li, Dechun Zeng, Shuai Yang, Baozhong Chen and Junbiao Peng, ","doi":"10.1021/acsomega.4c0999410.1021/acsomega.4c09994","DOIUrl":"https://doi.org/10.1021/acsomega.4c09994https://doi.org/10.1021/acsomega.4c09994","url":null,"abstract":"The trade-off between mobility and stability is the main problem for amorphous oxide semiconductor (AOS) thin-film transistors (TFTs). In this work, a high-mobility and good-photostability Tb-doped indium–tin–zinc oxide (Tb:ITZO) AOS is studied. The ITZO TFT without Tb doping had a mobility of as high as 49.4 cm2·V–1·s–1 but exhibited poor stability under negative bias illumination stress (NBIS). After Tb doping, the NBIS stability of the TFTs improved greatly, but the mobility reduced to 37.9 cm2·V–1·s–1. The great improvement of the NBIS stability of the Tb:ITZO TFTs is attributed to the charge-transfer transition between Tb4f7O2p6 and Tb4f8O2p5 that would reduce the lifetime of the photoinduced electrons and the ionized oxygen vacancies. Increasing O2 flow rates during sputtering would further enhance the NBIS stability but decrease the mobility seriously. To further improve the mobility of the Tb:ITZO TFTs, a stacked-channel TFT was fabricated by using the same Tb:ITZO target (sputtering with different O2 flow rates). The mobility increased to 40.4 cm2·V–1·s–1, and it also exhibited good NBIS stability. The mobility increment of the stacked-channel TFTs is ascribed to the conduction path confinement of the front channel by the conduction band barrier at the bilayer interface. This work provides a prominent AOS material and a simple way to break the trade-off between the mobility and stability of the AOS-TFTs.","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 8","pages":"8262–8270 8262–8270"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c09994","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting Anti-Stokes Emission Features of DNA-Stabilized Silver Nanoclusters

IF 3.7 3区化学

ACS Omega Pub Date : 2025-02-19 DOI: 10.1021/acsomega.4c1138410.1021/acsomega.4c11384

Mikkel Baldtzer Liisberg*, Giacomo Romolini, Vanessa Rück, Cecilia Cerretani and Tom Vosch*,

{"title":"Revisiting Anti-Stokes Emission Features of DNA-Stabilized Silver Nanoclusters","authors":"Mikkel Baldtzer Liisberg*, Giacomo Romolini, Vanessa Rück, Cecilia Cerretani and Tom Vosch*, ","doi":"10.1021/acsomega.4c1138410.1021/acsomega.4c11384","DOIUrl":"https://doi.org/10.1021/acsomega.4c11384https://doi.org/10.1021/acsomega.4c11384","url":null,"abstract":"DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of fluorophores with interesting photophysical properties. They are capable of generating anti-Stokes fluorescence upon excitation with near-infrared lasers. The anti-Stokes fluorescence has previously been speculated to be either the result of consecutive photon absorption (upconversion) or hot band absorption (HBA). Here, we revisit the anti-Stokes fluorescence of DNA-AgNCs to determine the underlying mechanistic origin. We investigate two previously studied DNA-AgNCs together with two organic fluorophores with absorption and emission features in the same spectral regions as the DNA-AgNCs. From the recorded anti-Stokes fluorescence excitation spectra, we find that all the emitters exhibit an exponential-like decaying slope on the red side of the lowest energy absorption band. Furthermore, excitation power dependency measurements at different excitation wavelengths verify the one-photon nature of the anti-Stokes fluorescence. Ultimately, the lack of discrete optical transition features in the anti-Stokes fluorescence excitation spectra suggests that the HBA mechanism is the most plausible cause for the anti-Stokes fluorescence of the investigated DNA-AgNCs.","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 8","pages":"8652–8657 8652–8657"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c11384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanostructured Lipid Carrier for Intracellular Delivery of a Bis(pyridine-2-carboxamidine) DNA Minor Groove Binder Active against Leishmania

IF 3.7 3区化学

ACS Omega Pub Date : 2025-02-19 DOI: 10.1021/acsomega.4c0813810.1021/acsomega.4c08138

J. Jonathan Nué-Martinez, Marta Leo-Barriga, Fernando Herranz, Zisis Koutsogiannis, Paul W. Denny, Godwin U. Ebiloma, Christophe Dardonville* and Ana González-Paredes*,

{"title":"Nanostructured Lipid Carrier for Intracellular Delivery of a Bis(pyridine-2-carboxamidine) DNA Minor Groove Binder Active against Leishmania","authors":"J. Jonathan Nué-Martinez, Marta Leo-Barriga, Fernando Herranz, Zisis Koutsogiannis, Paul W. Denny, Godwin U. Ebiloma, Christophe Dardonville* and Ana González-Paredes*, ","doi":"10.1021/acsomega.4c0813810.1021/acsomega.4c08138","DOIUrl":"https://doi.org/10.1021/acsomega.4c08138https://doi.org/10.1021/acsomega.4c08138","url":null,"abstract":"Two types of nanostructured lipid carrier (NLC) formulations of the antileishmanial DNA minor groove binding compound 4-(picolinimidamido)-N-(4-(picolinimidamido)phenyl)benzamide (1) were synthesized by the microemulsion method to evaluate their potential as a delivery system to intracellular forms of the Leishmania parasite. Compound 1-loaded NLC formulations, functionalized with folic acid (FA) or not, showed good colloidal stability both during storage and after dilution in biologically relevant media. The drug release, which was not pH dependent, occurred efficiently at 37 °C. Compound 1 and its NLC formulations displayed a good selectivity index (SI > 40) and were active in the submicromolar range against promastigotes and metacyclic promastigotes of Leishmania major and in the low micromolar range (∼2 μM) against intramacrophage amastigotes. However, they were inactive against Leishmania mexicana. This proof-of-concept study showed that compound 1 could be loaded effectively in NLC and FA-coated NLC whose size and anionic nature (i.e., FA-NLC) are favorable for uptake into macrophages. NLC functionalization with FA had a beneficial effect on the antileishmanial activity of 1 compared with noncoated NLC formulations, which resulted in an increase in selectivity toward the parasite.","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 8","pages":"7795–7805 7795–7805"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c08138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of B Content on Microstructure and Properties of CrFeNiAlSi High-Entropy Alloys

IF 3.7 3区化学

ACS Omega Pub Date : 2025-02-19 DOI: 10.1021/acsomega.4c0847410.1021/acsomega.4c08474

Ying Wen*, Jian Chen* and Gang Li,

{"title":"Effect of B Content on Microstructure and Properties of CrFeNiAlSi High-Entropy Alloys","authors":"Ying Wen*, Jian Chen* and Gang Li, ","doi":"10.1021/acsomega.4c0847410.1021/acsomega.4c08474","DOIUrl":"https://doi.org/10.1021/acsomega.4c08474https://doi.org/10.1021/acsomega.4c08474","url":null,"abstract":"A series of CrFeNiAlSiBx (x = 0.1, 0.3, 0.5, and 0.7) high-entropy alloys (HEAs) were proposed by vacuum arc melting. The study aimed to investigate the effect of boron (B) content on the microstructure evolution, phase formation regulation, wear, corrosion, and oxidation properties. The results show that the CrFeNiAlSiBx HEAs consisted of the BCC phase, (Cr, Fe)3Si phase, and (Cr, Fe)B compound. The HEAs had a dendritic morphology, with silicon-rich phases between the grains and AlNi phases within the grains, and B forms a needle-like second phase with Cr elements. The hardness of the HEAs was mainly strengthened by the second phase, with B0.3 HEA having the highest hardness of 1121 HV and the lowest wear per unit area of 0.97 mg·cm–2. In a 3.5 wt % NaCl solution, B0.3 HEA had the best corrosion resistance, with an annual corrosion rate of 0.043 mm/a. The increase of B content changed the second phase from the dispersed fine needle-like structure to the coarse flat noodles structure, which accelerates intergranular corrosion. The B element improved the high-temperature oxidation resistance of the alloy, reduced the oxidation rate of the alloy, and shortened the time for the HEAs to form a complete antioxidation film.","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 8","pages":"7813–7821 7813–7821"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c08474","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward Sustainable Radioactive Waste Management: Geopolymerization of Sewage Sludge Ash as a Viable Solution

IF 3.7 3区化学

ACS Omega Pub Date : 2025-02-19 DOI: 10.1021/acsomega.4c0719510.1021/acsomega.4c07195

Alexandre Las Casas*, Leandro Goulart de Araujo, Roberto Vicente and Júlio Takehiro Marumo,

{"title":"Toward Sustainable Radioactive Waste Management: Geopolymerization of Sewage Sludge Ash as a Viable Solution","authors":"Alexandre Las Casas*, Leandro Goulart de Araujo, Roberto Vicente and Júlio Takehiro Marumo, ","doi":"10.1021/acsomega.4c0719510.1021/acsomega.4c07195","DOIUrl":"https://doi.org/10.1021/acsomega.4c07195https://doi.org/10.1021/acsomega.4c07195","url":null,"abstract":"This study examines the geopolymerization potential of sewage sludge ash (SSA) for immobilizing radioactive waste through a series of experimental phases. The initial phase of the study involved processing sewage sludge from three different treatment plants, followed by calcination and subsequent characterization. The initial synthesis of geopolymers was conducted using 100% SSA, followed by compressive strength testing. In the second phase, a full factorial design was employed to optimize a metakaolin-based geopolymer formulation, with adjustments made to five variables: metakaolin (MK), activating solution (AS), sand, water, and lime. The optimal conditions were identified as 120 g MK, 125 g AS, 360 g sand, 55 g water, and 14.5 g lime. Under these conditions, the compressive strength increased from 15.0 ± 1.0 to 21.3 ± 0.6 MPa when the specimens were cured at 60 °C for 6 h. The optimized formulation was then augmented with SSA, and its characteristics were examined through a series of analytical techniques, including ICP–OES, XRF, XRD, SEM, and EDS. In the third phase of the study, immobilization of simulated radioactive activated carbon and ion-exchange resin wastes contaminated with 137Cs within the geopolymer matrix was investigated. Leaching and compressive strength tests were conducted to evaluate the performance of the material, and the results indicated that the release rates of 137Cs were between 2.55 × 10–5 and 3.23 × 10–5 cm d–1. These findings suggest that SSA-derived geopolymers can effectively immobilize radioactive waste, offering a sustainable alternative to traditional Portland cement.","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 8","pages":"7683–7696 7683–7696"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c07195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aromatic Residue F443 Modulates the Dimer Interface and Activity of Pseudomonas mandelii Glutathione Reductase

IF 3.7 3区化学

ACS Omega Pub Date : 2025-02-19 DOI: 10.1021/acsomega.5c0019210.1021/acsomega.5c00192

Oanh Mai Ho, Trang Hoang and ChangWoo Lee*,

{"title":"Aromatic Residue F443 Modulates the Dimer Interface and Activity of Pseudomonas mandelii Glutathione Reductase","authors":"Oanh Mai Ho, Trang Hoang and ChangWoo Lee*, ","doi":"10.1021/acsomega.5c0019210.1021/acsomega.5c00192","DOIUrl":"https://doi.org/10.1021/acsomega.5c00192https://doi.org/10.1021/acsomega.5c00192","url":null,"abstract":"Glutathione reductase (GR) is a homodimeric flavoenzyme that regenerates reduced glutathione, essential for cellular redox balance, with an active site cleft at the upper dimer interface. However, the role of intramolecular interactions within each monomer in maintaining the dimer interface and influencing enzyme activity remains unclear. This study investigates how hydrophobic interactions within each monomer near the dimer interface, particularly involving L416 (α12 helix) and F443 (α14 helix), affect the stability and activity of GR from the psychrotrophic bacterium Pseudomonas mandelii JR-1 (PmGR). Mutations at L416 (L416A, L416E, L416Q) preserved the dimeric form during native polyacrylamide gel electrophoresis at 4 and 50 °C but reduced catalytic function, with activity progressively declining at 50 and 60 °C. In contrast, F443A remained dimeric only at 4 °C, while F443E and L416A/F443A disrupted hydrophobic interactions, causing dimer dissociation at 4 °C and severely impairing enzyme activity. F443L, however, retained dimerization under all tested conditions. Despite these disruptions, all mutants except F443A retained their secondary structure. These findings demonstrate that L416 in the longer α12 helix modulates enzyme kinetics without destabilizing the dimer interface, whereas hydrophobic interactions involving F443 in the shorter α14 helix are critical for dimer integrity and activity. This study highlights the essential role of hydrophobic interactions within each monomer, particularly those involving F443, alongside dimer interface interactions, in maintaining GR stability and activity, offering valuable insights for GR engineering in oxidative stress defense.","PeriodicalId":22,"journal":{"name":"ACS Omega","volume":"10 8","pages":"8709–8717 8709–8717"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsomega.5c00192","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0