2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)最新文献

{"title":"Novel neural interface for modulation of neuronal activity based on millimeter wave exposure","authors":"V. Pikov, P. Siegel","doi":"10.1109/LISSA.2011.5754180","DOIUrl":"https://doi.org/10.1109/LISSA.2011.5754180","url":null,"abstract":"Realizing a minimally-invasive interface with the brain for treating neurological disorders represents a considerable challenge for modern medicine. Implantable neuromodulation devices have been successfully used for treating a variety of neurological disorders, such as Parkinson's disease, dystonia, epilepsy, chronic pain, and migraine. However, the implantation trauma and the risks associated with chronic presence of the implant in the nervous tissue have limited the widespread use of these devices. Minimally-invasive technologies based on the surface electrical stimulation and transcranial magnetic stimulation have been developed to modulate the neuronal excitability in the brain, but their spatial resolution, limited to several centimeters, is insufficient for selective stimulation of millimeter-scale neuronal populations in the brain. Here, we describe some unique benefits afforded by a novel non-contact neuromodulation technique that employs millimeter waves. Using the leech ganglion preparation, we demonstrate a profound suppression of neuronal excitability induced by a low-power millimeter wave exposure. Based on these findings, we discuss the possible mechanisms and implications for developing a minimally-invasive neuromodulation therapy.","PeriodicalId":227469,"journal":{"name":"2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125310206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications of activatable probes in molecular imaging","authors":"Lei Zhu, Xiaoyuan Chen","doi":"10.1109/LISSA.2011.5754174","DOIUrl":"https://doi.org/10.1109/LISSA.2011.5754174","url":null,"abstract":"Most new drug candidates generated during in vitro screenings turn out to be invalid after time-consuming and costly testing in animal models. Therefore, development of noninvasive, real-time, sensitive, and cost-effective tools with higher throughput for monitoring and early detection of drug efficacy in vivo is urgently needed. Of such techniques, optical molecular imaging provides many advantages over other imaging modalities, including the use of non-radioactive materials, high sensitivity, and safe detection using readily available instruments at moderate cost. Performance of in vivo optical imaging is solely dependent on the development of sophisticated imaging probes that exhibit high sensitivity and low background noise. Among diverse applications, peptide-based molecular beacons, so called protease activatable optical probes, have enabled in vivo imaging of proteases activity and demonstrated promising results in the field of protease research and protease-targeted drug development. Matrix metalloproteinases (MMPs) are a family of Zn2+ dependent endopeptidase. It is highly expressed in most cancers, cardiovascular diseases and other diseases. Herein, we are using MMPs as targets to develop an ultra-sensitive activatable probe for throughput drug discovery application.","PeriodicalId":227469,"journal":{"name":"2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)","volume":"186 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132710064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combinational portable raman probes: C-nanotubes for theranostics application","authors":"Ashwinkumar A. Bhirde, Xiaoyuan Chen","doi":"10.1109/LISSA.2011.5754185","DOIUrl":"https://doi.org/10.1109/LISSA.2011.5754185","url":null,"abstract":"Recently portable Raman probes have emerged into markets with a variety of applications including carbon nanotube (CNTs) characterization. Aqueous dispersed carbon nanotubes (CNTs) have shown a lot of promise towards biomedical application like drug / gene delivery vectors, photo-thermal therapy, and photoacoustic imaging. In this study we report the simultaneous detection and irradiation of carbon nanotubes in live cancer cells using a portable Raman probe. A portable handheld Raman instrument was utilized for dual purpose, as a CNT detector and as an irradiating laser source. Single walled carbon nanotubes (SWCNTs) and multiwalled carbon nanotubes (MWCNTs) were dispersed in an aqueous solution using a lipid-polymer (LP) coat which formed highly stable dispersions both in buffer and cell media. The LP coated SWCNTs and MWCNTs aqueous dispersions were characterized by atomic force microscopy, transmission electron microscopy, dynamic light scattering, Fourier transform infrared spectroscopy and Raman spectroscopy. The cellular uptake of the LP dispersed SWCNTs and MWCNTs was carried out using confocal microscopy, where the FITC labeled nanotube conjugates were found internalized by the breast cancer cells confirmed by Z-stack fluorescence confocal imaging. The in vitro biocompatibility of SWCNTs and MWCNTs, assessed using cell viability MTT assay, found that the nanotube dispersions did not hinder the cell proliferation of breast cancer cells at the dosages tested. Breast cancer cells treated with SWCNTs and MWCNTs were simultaneously detected and irradiated live in vitro using the portable Raman probe. Apoptotic TUNEL assay carried out on the breast cancer cells fixed after laser irradiation confirmed the cell death only in presence of the nanotube dispersions. For the first time we show that both SWCNTs and MWCNTs could be selectively irradiated by specifically detecting the CNTs in cancer cells using a simple handheld Raman instrument in three dimensionally grown cell culture. A combination of handheld Raman instrumentation used along with carbon nanomaterials could help treat various diseases like cancer.","PeriodicalId":227469,"journal":{"name":"2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116894795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Honey based fibrous scaffold for tissue engineering application","authors":"A. Barui, P. Banerjee, Raunak KumarDas, S. Dhara, J. Chatterjee","doi":"10.1109/LISSA.2011.5754161","DOIUrl":"https://doi.org/10.1109/LISSA.2011.5754161","url":null,"abstract":"Natural product like honey is well known for its compositional diversity, medicinal properties including anti-inflammatory role, wound repair efficacy and nutritional competence. This multi-dimensional character of honey attracts regenerative medicine researchers. However, flow behavior of honey restricts its applications as a sustainable biomaterial matrix. In this juncture this study develops honey-biomaterial formulations and fabricates fibrous scaffold through wet spinning technique towards regenerative medicine applications. The cell culture study demonstrates the bio-compatibility of the fabricated constructs for fibroblasts (3T3) and keratinocyte (HaCaT) through MTT assay and by scanning electron microscopy regarding adhesion and penetration of cells on the scaffold. Better performance has been exhibited by the honey-biomaterial based scaffolds in comparison to alginate fibrous construct. This study also shows better expression of prime molecules like collagen I and III in 3T3 and p63 and E-cadherin in HaCaT on honey based matrix than that of alginate matrix or control group. Thus present work develops a honey-based fibrous matrix with biocompatible interface for plausible tissue engineering applications.","PeriodicalId":227469,"journal":{"name":"2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)","volume":"283 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114157530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low cost semi-confocal molecular imaging with cold light source","authors":"R. Das, A. Barui, C. Chakraborty, A. Ray, J. Chatterjee, M. Pal, R. Paul","doi":"10.1109/LISSA.2011.5754167","DOIUrl":"https://doi.org/10.1109/LISSA.2011.5754167","url":null,"abstract":"Imaging bio-molecules especially in tissues, cells and even in in-vitro live conditions require expertise not only in staining and tagging of the particular molecules of interest but also their imaging so as to make any meaningful analysis and quantification of the obtained images. This necessitates a microscopic facility which can grab fluorescent images with minimum bleaching of the dyes and gives clear and crisp images without the blur caused by out of focus light. In this context ApoTome provides an alternative with metal halide light source and structural illumination with the help of grids, along with integrated image processing modality to generate images with digital interface. In the current study ApoTome was used to grab images of p63 and E-cadherin on histological sections of Oral Submucous Firosis an oral pre-cancer. The obtained images could aptly localize the expression of p63 and E-cadherin and distinguish the early and advanced stages of the precancer and did not show any effect of photo-bleaching. This demonstrated the applicability of ApoTome and its potential impact on diagnostics.","PeriodicalId":227469,"journal":{"name":"2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125049184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable materials and systems for electronically active device-based tissue regeneration","authors":"C. Bettinger","doi":"10.1109/LISSA.2011.5754177","DOIUrl":"https://doi.org/10.1109/LISSA.2011.5754177","url":null,"abstract":"Studies of biodegradable materials and devices for potential use as temporary resorbable electronic medical implants are presented. The integration of electronic functionality within biomaterials is a powerful avenue to interface therapeutic medical implants with host tissue. Such devices could be integrated with telemetry to remotely stimulate tissue, monitor cell function, or dynamically deliver drugs, for example. Silicon-based devices exhibit many advantages for these applications. However, these materials often produce deleterious interactions with soft tissues. Traditional medical biomaterials offer advantageous biocompatibility and biodegradability, but often lack the appropriate electronic properties for these applications. Hence, the potential to use melanin, a natural semiconducting pigment, as an electronic biomaterial was evaluated. Melanin exhibits adequate electronic conductivities, exceptional biocompatibility and in vivo degradation. This naturally occurring organic semiconductor may provide a suitable electronic biomaterial interface. Biodegradable materials have the potential to serve as structural and functional materials in electronically active devices. Organic thin film transistor structures using a primarily biodegradable material platform were fabricated and the electronic performance of these devices was evaluated. These devices performed stably after exposure to water and were completely resorbed after 50 days in vitro. These studies collectively demonstrate the potential to integrate biodegradable materials with electronic functionality for therapeutic applications including regenerative medicine.","PeriodicalId":227469,"journal":{"name":"2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131049735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simple and sensitive cytosensor based electrical characterization of in vitro wound healing assay for keratinocytes","authors":"N. Mondal, D. Mondal, C. RoyChaudhuri, A. Barui, S. Dhara, J. Chatterjee","doi":"10.1109/LISSA.2011.5754152","DOIUrl":"https://doi.org/10.1109/LISSA.2011.5754152","url":null,"abstract":"Confluent monolayers of cells in culture media are usually fragile and are susceptible to mechanical disruption. To assess the growth and migration of the cells towards recovery, the mechanical disruption is often done deliberately to perform wound healing assay. In such analysis, after a scratch in the cell monolayer, electrical characterization has been done to provide related quantitative description of the cellular behavior compared to the microscopic observation. In this direction for cellular electrical characterization, biosensors are usually designed with photolithographically patterned electrodes which are of the dimensions of the cells. This increases the cost and complexity of the analysis. Here we report the electrical characterization of in vitro wound healing for keratinocytes monolayer in DMEM-F12 medium with a low cost and sensitive macroporous silicon platform using simple electrode geometries for the first time. Impedance spectroscopy results show that there is a distinct difference between the electrical properties like the effective capacitance and the resistance of the keratinocytes (HaCaT) in the frequency range from 100Hz to 1MHz at two different time instants after wounding. The difference in the electrical properties has been qualitatively explained with the microscopic and immunocytochemical findings. This analysis may help to assess the cell behaviour during its growth and repair through a less complex and low cost electrical route.","PeriodicalId":227469,"journal":{"name":"2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)","volume":"476 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123556264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shape effect of mesoporous silica nanoparticles on cellular and in vivo functions","authors":"Xinglu Huang, Xiaoyuan Chen","doi":"10.1109/LISSA.2011.5754158","DOIUrl":"https://doi.org/10.1109/LISSA.2011.5754158","url":null,"abstract":"The design of smart functional nanosystems for theranostics requires a thorough understanding of the interaction between nanoparticles (NPs) and biological systems. Recent observations suggest that physical parameters of NPs, like size, shape and surface charge, can dramatically affect the behaviors of NPs in biological systems and might, in part, determine the bioapplications of NPs. Among various factors, particle shape has been considered to play an important role in both cellular interactions with NPs and systemic distribution of NPs. However, limited information is available regarding the effects of particle shape on these biological behaviors.","PeriodicalId":227469,"journal":{"name":"2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131102523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pressure ulcer prevention: An efficient turning schedule for bed-bound patients","authors":"S. Ostadabbas, R. Yousefi, M. Faezipour, M. Nourani, M. Pompeo","doi":"10.1109/LISSA.2011.5754183","DOIUrl":"https://doi.org/10.1109/LISSA.2011.5754183","url":null,"abstract":"Pressure ulcer is a critical problem for bed-ridden and wheelchair-bound patients, diabetics, and the elderly. Patients need to be regularly repositioned to prevent excessive pressure on a single area of body, which can lead to ulcers. Pressure ulcers are costly to treat and cause many other health problems, including death. The current standard for prevention is to reposition at-risk patients every two hours. This level of attention is becoming increasingly unrealistic for already overworked nursing staff. In this paper, we present a scheduling algorithm that uses data from a pressure mat on the hospital bed to compute a repositioning schedule that minimizes nursing staff interaction while still preventing pressure ulcer formation. Our experimental results show a 30% increase in the average time between repositioning over the standard schedule. Furthermore, some postures were found to be unsafe if not changed for more than one hour.","PeriodicalId":227469,"journal":{"name":"2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117190376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A biomimetic fabricated carbon nanotube synapse for prosthetic applications","authors":"J. Joshi, Jialu Zhang, Chuan Wang, Chih-Chieh Hsu, A. C. Parker, Chongwu Zhou, Udhay Ravishankar","doi":"10.1109/LISSA.2011.5754178","DOIUrl":"https://doi.org/10.1109/LISSA.2011.5754178","url":null,"abstract":"A biomimetic carbon nanotube synapse, the portion of the neuron that receives inputs from other neurons, has been fabricated in the laboratory as an analog circuit. The waveforms input to the synapse and output from the synapse resemble biological waveforms in shape and relative amplitudes and durations. This working circuit is an important first step towards the use of nanotechnology for biomimetic neural circuits and neural prosthesis.","PeriodicalId":227469,"journal":{"name":"2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128773001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}