The Journal of applied bacteriology最新文献_第6页

A red pigment synthesized by an Aspergillus parasiticus mutant as a possible new intermediate in the aflatoxin biosynthetic pathway. 寄生曲霉突变体合成的一种红色色素，可能是黄曲霉毒素生物合成途径的新中间体。

The Journal of applied bacteriology Pub Date : 1994-11-01 DOI: 10.1111/j.1365-2672.1994.tb04401.x

M E García, M D Herce, J L Blanco, G Suárez

引用次数: 2

A note: comparison of different homogenization procedures for detecting Campylobacter spp. in sewage sludge. 注:在污水污泥中检测弯曲杆菌的不同均质程序的比较。

The Journal of applied bacteriology Pub Date : 1994-11-01 DOI: 10.1111/j.1365-2672.1994.tb04406.x

C Höller, U Schomakers-Revaka

引用次数: 9

Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes. 多态细菌DNA的随机扩增:11种寡核苷酸的评价及其在单核增生李斯特菌污染食品中的应用。

The Journal of applied bacteriology Pub Date : 1994-11-01 DOI: 10.1111/j.1365-2672.1994.tb04404.x

C Niederhauser, C Höfelein, M Allmann, P Burkhalter, J Lüthy, U Candrian

{"title":"Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes.","authors":"C Niederhauser, C Höfelein, M Allmann, P Burkhalter, J Lüthy, U Candrian","doi":"10.1111/j.1365-2672.1994.tb04404.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1994.tb04404.x","url":null,"abstract":"The polymerase chain reaction was used to obtain randomly-amplified polymorphic DNA (RAPD) profiles from Listeria spp. and enterobacteria. Eleven different oligonucleotides were evaluated. Only one, HR4 (19mer), generated reproducible and specific profiles for Listeria spp., while results for enterobacteria were controversial. A total of 57 different Listeria strains were subjected to the RAPD analysis and 27 different profiles were recognized. RAPD typing allowed strains of the same serotype to be distinguished but the same profile was obtained from different serotypes of L. monocytogenes in three cases and in one case two different serotypes of L. innocua yielded the same profile. RAPD-typing with HR4 allowed L. monocytogenes contamination in several food outlets to be traced back to a food processing plant. In additional experiments, the general utility of this RAPD system in typing Yersinia enterocolitica, verotoxigenic Escherichia coli and Salmonella enteritidis was evaluated.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"77 5","pages":"574-82"},"PeriodicalIF":0.0,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1994.tb04404.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18997601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Characterization of beta-glucosidase activity in yeasts of oenological origin. 酿酒源酵母β -葡萄糖苷酶活性的表征。

The Journal of applied bacteriology Pub Date : 1994-11-01 DOI: 10.1111/j.1365-2672.1994.tb04396.x

I Rosi, M Vinella, P Domizio

{"title":"Characterization of beta-glucosidase activity in yeasts of oenological origin.","authors":"I Rosi, M Vinella, P Domizio","doi":"10.1111/j.1365-2672.1994.tb04396.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1994.tb04396.x","url":null,"abstract":"Three hundred and seventeen strains representing 20 species of yeasts were screened for the presence of beta-glucosidase activity. All of the strains of the species Debaryomyces castellii, Deb. hansenii, Deb. polymorphus, Kloeckera apiculata and Hansenula anomala showed beta-glucosidase activity, but only one of 153 strains of Saccharomyces cerevisiae. The other species behaved differently, depending upon the strain. The strains that hydrolysed arbutin were checked to localize the beta-glucosidase activity. A strain of Deb. hansenii exhibited the highest exocellular activity and some wall-bound and intracellular activity. The beta-glucosidase synthesis from this yeast was enhanced by aerobic conditions of growth, was repressed by high glucose concentration (9%) and occurred during exponential growth. The optimum conditions for enzymatic preparations of Deb. hansenii were between pH 4.0 and 5.0 and 40 degrees C. A high concentration of ethanol and glucose did not reduce the enzymatic activity. The enzymatic preparations of Deb. hansenii released monoterpenols and other alcohols from a grape glycoside extract.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"77 5","pages":"519-27"},"PeriodicalIF":0.0,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1994.tb04396.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18997597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 230

The genetic analysis of bacterial spore germination. 细菌孢子萌发的遗传分析。

The Journal of applied bacteriology Pub Date : 1994-09-01

A Moir, E H Kemp, C Robinson, B M Corfe

引用次数: 0

Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures. 五株沙门菌在不同温度刺培养中质粒的稳定性。

The Journal of applied bacteriology Pub Date : 1994-08-01 DOI: 10.1111/j.1365-2672.1994.tb03059.x

J E Olsen, D J Brown, D L Baggesen, M Bisgaard

{"title":"Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures.","authors":"J E Olsen, D J Brown, D L Baggesen, M Bisgaard","doi":"10.1111/j.1365-2672.1994.tb03059.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1994.tb03059.x","url":null,"abstract":"Four strains of Salmonella berta and one of Salm. enteritidis were stored as stab cultures in sugar-free agar at 5 degrees, 22 degrees and 30 degrees C and in 15% glycerol at -80 degrees C. The stability of the plasmid profiles in each of the strains was monitored over a period of 2.5 years. Plasmid profiles were stable in all strains stored at -80 degrees C, and only six of 450 colonies examined from strains kept in sugar-free agar at 5 degrees C had lost plasmid molecules. Seventy of 440 colonies from stab cultures that were kept at 22 degrees C, and 71 of 440 colonies at 30 degrees C showed changed plasmid profiles. The total number of plasmids lost increased with time, and occasionally, more than one plasmid molecule was lost in the same strain. The virulence associated plasmid of Salm. enteritidis was remarkably stable as it was maintained in all colonies examined at all temperatures investigated. Likewise, no change in SmaI restriction profile was observed in this plasmid molecule at any temperature.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"77 2","pages":"155-9"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1994.tb03059.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18956423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Evaluation of determinative tests for pathovars of Pseudomonas syringae van Hall 1902. 丁香假单胞菌病原菌测定方法的评价。

The Journal of applied bacteriology Pub Date : 1994-08-01 DOI: 10.1111/j.1365-2672.1994.tb03064.x

J M Young, C M Triggs

{"title":"Evaluation of determinative tests for pathovars of Pseudomonas syringae van Hall 1902.","authors":"J M Young, C M Triggs","doi":"10.1111/j.1365-2672.1994.tb03064.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1994.tb03064.x","url":null,"abstract":"The utility of 36 presumptive determinative tests for 32 pathovars of Pseudomonas syringae was investigated. A total of 395 strains was examined. Most strains of 12 of these pathovars (Ps. syringae pv. cannabina, Ps. syr. delphinii, Ps. syr. glycinea, Ps. syr. helianthi, Ps. syr. lachrymans, Ps. syr. mori, Ps. syr. morsprunorum, Ps. syr. phaseolicola, Ps. syr. 'porri', Ps. syr. papulans, Ps. syr. savastanoi and Ps. syr. tabaci) formed clusters when test data were compared by centroid analysis. Pseudomonas syr. syringae, Ps. syr. aptata, Ps. syr. atrofaciens, Ps. syr. dysoxyli and Ps. syr. japonica formed a single cluster, indicating their possible synonymy. Strains of Ps. syr. antirrhini and Ps. syr. tomato were indistinguishable, as were those of Ps. syr. garcae and Ps. syr. oryzae. Strains of Ps. syr. berberidis, Ps. syr. coronafaciens, Ps. syr. eriobotryae, Ps. syr. maculicola, Ps. syr. passiflorae, Ps. syr. pisi and Ps. syr. striafaciens and Ps. syr. tagetis did not form distinguishable clusters. The tests which reliably differentiated pathovars are recorded in a determinative scheme.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"77 2","pages":"195-207"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1994.tb03064.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18956426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 81

The effect of Lactobacillus spp. on the attachment of enterotoxigenic Escherichia coli to isolated porcine enterocytes. 乳酸菌对产肠毒素大肠杆菌在分离猪肠细胞上附着的影响。

The Journal of applied bacteriology Pub Date : 1994-08-01 DOI: 10.1111/j.1365-2672.1994.tb03066.x

R J Spencer, A Chesson

{"title":"The effect of Lactobacillus spp. on the attachment of enterotoxigenic Escherichia coli to isolated porcine enterocytes.","authors":"R J Spencer, A Chesson","doi":"10.1111/j.1365-2672.1994.tb03066.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1994.tb03066.x","url":null,"abstract":"A total of 43 strains of lactobacilli were isolated from the gastrointestinal tract of piglets at the time of weaning. Isolates, grown on solid media, were allocated to strongly adherent or non/weakly adherent groups on the basis of numbers attaching to isolated porcine enterocytes. Strains of Lactobacillus fermentum were disproportionally represented amongst the strongly-adherent strains and Lact. acidophilus and Lact. salivarius amongst the non/weakly-adherent group. Lactobacilli showed significantly better attachment ability when grown on agar than when grown in broth culture. Strongly adherent strains were not found to effect the attachment of enterotoxigenic Escherichia coli to porcine enterocytes, tested under the conditions of exclusion (lactobacilli added to the enterocytes before E. coli), competition (lactobacilli and E. coli added simultaneously) and displacement (E. coli added before lactobacilli). Tests were made with [14C]-labelled E. coli. Suspensions of bacteria and enterocytes were passed through a filter selected to retain enterocytes but pass free bacterial cells. Counts (dpm) obtained from filters after solubilization were taken as a measure of E. coli attachment. Some strains of lactobacilli coaggregated with enterotoxigenic E. coli with K88 fimbriae, but not with a K88-negative mutants strain. These were excluded from the competitive exclusion experiments. In the apparent absence of a direct effect on the association of E. coli with host tissue, removal of potential gut pathogens by aggregation could contribute to the probiotic properties ascribed to lactic acid bacteria.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"77 2","pages":"215-20"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1994.tb03066.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18956428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 115

Antimicrobial activity of lactobacilli: preliminary characterization and optimization of production of acidocin B, a novel bacteriocin produced by Lactobacillus acidophilus M46. 乳酸菌的抑菌活性:嗜酸乳杆菌M46产生的一种新型细菌素酸素B的初步鉴定和生产优化。

The Journal of applied bacteriology Pub Date : 1994-08-01 DOI: 10.1111/j.1365-2672.1994.tb03057.x

B ten Brink, M Minekus, J M van der Vossen, R J Leer, J H Huis in't Veld

{"title":"Antimicrobial activity of lactobacilli: preliminary characterization and optimization of production of acidocin B, a novel bacteriocin produced by Lactobacillus acidophilus M46.","authors":"B ten Brink, M Minekus, J M van der Vossen, R J Leer, J H Huis in't Veld","doi":"10.1111/j.1365-2672.1994.tb03057.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1994.tb03057.x","url":null,"abstract":"Approximately 1000 lactobacillus strains were isolated and screened for the production of antimicrobial activity, using a target panel of spoilage organisms and pathogens. Only eight positive strains were found; two of these were studied in more detail. Lactobacillus salivarius M7 produces the new broad spectrum bacteriocin salivaricin B which inhibits the growth of Listeria monocytogenes, Bacillus cereus, Brochothrix thermosphacta, Enterococcus faecalis and many lactobacilli. A new atypical bacteriocin produced by Lact. acidophilus M46, acidocin B, combines the inhibition of Clostridium sporogenes with a very narrow activity spectrum within the genus Lactobacillus and was selected for further characterization. Acidocin B is sensitive to trypsin, heat-stable (80 degrees C for 20 min) and can be extracted from the culture supernatant fluid with butanol. Native acidocin B occurs as a large molecular weight complex (100 kDa), while with SDS-PAGE the partly purified activity migrates as a peptide of 2.4 kDa. Optimization of the cultivation conditions resulted in an eightfold increase of the amount of acidocin B produced during growth. Growth is not necessary for acidocin B production; washed producer cells can synthesize the bacteriocin in a chemically defined production medium. The application potential of acidocin B is discussed.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"77 2","pages":"140-8"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1994.tb03057.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18958378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 172

Lactoferricin, a new antimicrobial peptide. 乳铁蛋白，一种新型抗菌肽。

The Journal of applied bacteriology Pub Date : 1994-08-01 DOI: 10.1111/j.1365-2672.1994.tb03065.x

E M Jones, A Smart, G Bloomberg, L Burgess, M R Millar

{"title":"Lactoferricin, a new antimicrobial peptide.","authors":"E M Jones, A Smart, G Bloomberg, L Burgess, M R Millar","doi":"10.1111/j.1365-2672.1994.tb03065.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1994.tb03065.x","url":null,"abstract":"Lactoferricin B (LF-B) is a peptide derived from acid-pepsin digestion of bovine lactoferrin, which has antimicrobial properties. In order to assess the antimicrobial spectrum of LF-B and its possible in vivo uses, the minimum inhibitory and microbicidal concentrations of pure lactoferricin B were determined for a range of bacterial species and under varying conditions of growth including growth phase and size of the inoculum, pH and ionic strength of the medium. Lactoferricin B was bactericidal against a wide range of bacteria and Candida albicans. Proteus spp., Pseudomonas cepacia and Serratia spp. were resistant. The bactericidal activity of LF-B was inhibited by increasing ionic strength and bacterial inoculum and at acid pH. The activity of lactoferricin B was completely inhibited by the addition of 5% whole cow's milk and was reduced in the presence of increasing concentrations of mucin. These results indicate the potential of LF-B to reduce the numbers of organisms in a simple medium, but raise doubts about its role in vivo because of its sensitivity to changes in physical variables. It may be that lactoferricin exerts a transient antimicrobial effect at mucosal surfaces.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"77 2","pages":"208-14"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1994.tb03065.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18956427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 123