The Journal of applied bacteriology最新文献_第5页

Antibacterial effect of protamine assayed by impedimetry. 障碍法测定鱼精蛋白的抑菌效果。

The Journal of applied bacteriology Pub Date : 1995-03-01 DOI: 10.1111/j.1365-2672.1995.tb05029.x

C Johansen, T Gill, L Gram

{"title":"Antibacterial effect of protamine assayed by impedimetry.","authors":"C Johansen, T Gill, L Gram","doi":"10.1111/j.1365-2672.1995.tb05029.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1995.tb05029.x","url":null,"abstract":"Impedimetric measurements were used to assay the antibacterial effect of protamine. A good linear correlation between the impedance detection time and the initial cell counts was obtained (r = 0.99, n = 2). As basic peptides may cause clumping of cells, this correlation curve was used when estimating the cell number after protamine treatment, rather than colony counts. Protamine from salmon killed growing Gram-positive bacteria and significantly inhibited growth of Gram-negative bacteria in Tryptone Soy Broth (TSB) at 25 degrees C. In general Gram-positive bacteria were more sensitive to protamine than Gram-negative bacteria; the minimum inhibitory concentrations (MIC) determined for Gram-positive strains varied from 20 to 1000 micrograms ml-1 and for Gram-negative strains from 500 micrograms ml-1 to more than 4000 micrograms ml-1. The effect of protamine on non-growing Listeria monocytogenes Scott A suspended in buffer was not lethal as was the effect on growing cells; however, protamine (50-500 micrograms ml-1) killed the Gram-negative fish spoilage bacteria Shewanella putrefaciens when the live cells were suspended in buffer.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"78 3","pages":"297-303"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1995.tb05029.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18732526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 65

Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. 互花千层挥发油主要成分的抗菌活性研究。

The Journal of applied bacteriology Pub Date : 1995-03-01 DOI: 10.1111/j.1365-2672.1995.tb05025.x

C F Carson, T V Riley

{"title":"Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia.","authors":"C F Carson, T V Riley","doi":"10.1111/j.1365-2672.1995.tb05025.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1995.tb05025.x","url":null,"abstract":"Tea tree oil, or the essential oil of Melaleuca alternifolia, is becoming increasingly popular as a naturally occurring antimicrobial agent. The antimicrobial activity of eight components of tea tree oil was evaluated using disc diffusion and broth microdilution methods. Attempts were also made to overcome methodological problems encountered with testing compounds which have limited solubility in aqueous media. After assessing media with and without solubilizing agents, the disc diffusion method was used to determine the susceptibility of a range of micro-organisms to 1,8-cineole, 1-terpinen-4-ol, rho-cymene, linalool, alpha-terpinene, gamma-terpinene, alpha-terpineol and terpinolene. While the disc diffusion method lacked reproducibility, it was considered useful as a procedure for screening for antimicrobial activity. Terpinen-4-ol was active against all the test organisms while rho-cymene demonstrated no antimicrobial activity. Linalool and alpha-terpineol were active against all organisms with the exception of Pseudomonas aeruginosa. Minimum inhibitory and minimum cidal concentrations of each component against Candida albicans, Escherichia coli and Staphylococcus aureus were determined using a broth microdilution method. Modifications to this method overcame solubility and turbidity problems associated with the oil components and allowed the antimicrobial activity of each of the components to be quantified reproducibly. There was reasonable agreement between minimum inhibitory concentrations and zones of inhibition. These results may have significant implications for the future development of tea tree oil as an antimicrobial agent.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"78 3","pages":"264-9"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1995.tb05025.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18731907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 668

Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test. 基于DNA探针、PCR和活性检测的组氨酸脱羧乳酸菌检测系统的建立。

The Journal of applied bacteriology Pub Date : 1995-03-01 DOI: 10.1111/j.1365-2672.1995.tb05032.x

C Le Jeune, A Lonvaud-Funel, B ten Brink, H Hofstra, J M van der Vossen

{"title":"Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test.","authors":"C Le Jeune, A Lonvaud-Funel, B ten Brink, H Hofstra, J M van der Vossen","doi":"10.1111/j.1365-2672.1995.tb05032.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1995.tb05032.x","url":null,"abstract":"On the basis of the comparison of the nucleotide sequences of the histidine decarboxylase genes (hdcA) of Lactobacillus 30A and Clostridium perfringens and the amino acid sequences of these histidine decarboxylases and those of Lactobacillus buchneri and Micrococcus, oligonucleotides unique to the hdcA genes were synthesized and used in PCR. All histidine-decarboxylating lactic acid bacteria gave a signal with primer set JV16HC/JV17HC in PCR. In addition to this primer set, CL1/CL2 and CL1/JV17HC were also useful for the detection of histamine-forming Leuconostoc aenos strains in PCR. The 150 base pair amplification product of the decarboxylating Leuc. aenos strain generated with primer set CL1/CL2 was sequenced. Alignment studies showed a high degree of relatedness among the hdcA gene products of Gram-positive bacteria. The amplification products of the hdcA genes from Lac. buchneri and Leuct. aenos were used to serve as a DNA probe in hybridization studies. All histidine-decarboxylating lactic acid bacteria gave a hybridization signal with the DNA probes. In hybridization only one false-positive signal with a Lactobacillus lindneri strain was observed, which was anticipated to contain a truncated hdcA gene. In addition to these DNA probe tests, a simple and reliable activity test is presented, which can be used during starter selection to test strains for histidine decarboxylase activity.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"78 3","pages":"316-26"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1995.tb05032.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18732527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 132

Partial purification and characterization of bacteriocin from Yersinia kristensenii. 克里斯顿氏耶尔森菌细菌素的部分纯化及特性研究。

The Journal of applied bacteriology Pub Date : 1995-03-01 DOI: 10.1111/j.1365-2672.1995.tb05020.x

S Toora

引用次数: 9

Activity of p-aminobenzoic acid compared with other organic acids against selected bacteria. 对氨基苯甲酸与其他有机酸对选定细菌的活性比较。

The Journal of applied bacteriology Pub Date : 1995-03-01 DOI: 10.1111/j.1365-2672.1995.tb05018.x

R M Richards, D K Xing, T P King

{"title":"Activity of p-aminobenzoic acid compared with other organic acids against selected bacteria.","authors":"R M Richards, D K Xing, T P King","doi":"10.1111/j.1365-2672.1995.tb05018.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1995.tb05018.x","url":null,"abstract":"The antibacterial activity of p-aminobenzoic acid against Listeria monocytogenes, Salmonella enteritidis and Escherichia coli was compared with the activity of commonly used acidulants: formic, propionic, acetic, lactic and citric acids. Viable count evaluations and MIC determinations indicated that p-aminobenzoic acid caused greater inhibitory effects than the other organic acids. The activity of p-aminobenzoic acid on the growth of the test organisms at selected pH values indicated that p-aminobenzoic acid was more active at low pH than at high pH. Uptake studies showed that the uptake of p-aminobenzoic acid by E. coli was markedly decreased as the pH values increased. Electron micrographs of E. coli cells grown in the presence of p-aminobenzoic acid indicate that p-aminobenzoic acid caused marked damage to the cell envelope. It is suggested that p-aminobenzoic acid has at least two mechanisms of action: one mechanism in common with other organic acids and the other mechanism by interfering with the synthesis of the peptidoglycan layer by an action on the dihydrofolate reductase enzyme.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"78 3","pages":"209-15"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1995.tb05018.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18731903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59

Numerical characterization study of Micrococcaceae associated with lamb spoilage. 羊肉腐败相关微球菌科菌的数值表征研究。

The Journal of applied bacteriology Pub Date : 1995-03-01 DOI: 10.1111/j.1365-2672.1995.tb05024.x

M Prieto, M R García-Armesto, C González, A Otero, M L García López

引用次数: 2

Selective action of inhibitors used in different culture media on the competitive microflora of Salmonella. 不同培养基中抑制剂对沙门氏菌竞争菌群的选择性作用。

The Journal of applied bacteriology Pub Date : 1995-03-01 DOI: 10.1111/j.1365-2672.1995.tb05027.x

G Arroyo, J A Arroyo

引用次数: 14

Degradation and utilization of forage hemicellulose by rumen bacteria, singly in coculture or added sequentially. 瘤胃细菌对饲料半纤维素的降解和利用，可单独共培养或依次添加。

The Journal of applied bacteriology Pub Date : 1994-11-01 DOI: 10.1111/j.1365-2672.1994.tb04399.x

M Fondevila, B A Dehority

{"title":"Degradation and utilization of forage hemicellulose by rumen bacteria, singly in coculture or added sequentially.","authors":"M Fondevila, B A Dehority","doi":"10.1111/j.1365-2672.1994.tb04399.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1994.tb04399.x","url":null,"abstract":"Procedures for sequential addition experiments were developed to study the mechanisms involved in the synergistic and inhibitory interactions observed in forage hemicellulose digestion by rumen bacterial cocultures. One organism was allowed to ferment a forage substrate, the culture tube was sterilized and then inoculated with a second organism. No differences were found in the extent of degradation or utilization between fermentations sterilized by oxidation or heat, and based on ease of handling, heat was used in all subsequent experiments. Studies were conducted with Fibrobacter succinogenes A3c, Ruminococcus flavefaciens B34b and Prevotella ruminicola H2b, singly and in all possible combinations. Results from the sequential addition studies substantiated earlier suggestions that the increase observed in hemicellulose utilization results from initial solubilization of the hemicellulose from the forage by the non-utilizer and subsequent utilization of subsequent utilization of this solubilized polysaccharide by the utilizing, but non-degrading organism.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"77 5","pages":"541-8"},"PeriodicalIF":0.0,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1994.tb04399.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18997598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

The microaerophily and photosensitivity of Propionibacterium acnes. 痤疮丙酸杆菌的微气性和光敏性。

The Journal of applied bacteriology Pub Date : 1994-11-01 DOI: 10.1111/j.1365-2672.1994.tb04405.x

E M Gribbon, J G Shoesmith, W J Cunliffe, K T Holland

引用次数: 38

Staphylococcal growth and enterotoxins (A-D) and thermonuclease synthesis in the presence of dehydrated garlic. 脱水大蒜对葡萄球菌生长、肠毒素(A-D)和热酶合成的影响。

The Journal of applied bacteriology Pub Date : 1994-11-01 DOI: 10.1111/j.1365-2672.1994.tb04400.x

E González-Fandos, M L García-López, M L Sierra, A Otero

引用次数: 49