瘤胃细菌对饲料半纤维素的降解和利用,可单独共培养或依次添加。

M Fondevila, B A Dehority
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引用次数: 34

摘要

为了研究在瘤胃细菌共培养中观察到的饲料半纤维素消化的协同和抑制相互作用的机制,建立了顺序添加实验程序。一种微生物在草料基质上发酵,对培养管进行灭菌,然后用另一种微生物接种。氧化灭菌和加热灭菌的发酵在降解和利用程度上没有差异,基于易于处理,在随后的所有实验中都使用了加热。对琥珀酸原纤维杆菌A3c、黄瘤球菌B34b和反刍杆菌普雷沃菌H2b进行了单独和所有可能的组合研究。顺序添加研究的结果证实了先前的建议,即半纤维素利用率的增加是由于非利用者对饲料中的半纤维素的初始溶解作用以及利用但不降解的生物对这种被溶解的多糖的后续利用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Degradation and utilization of forage hemicellulose by rumen bacteria, singly in coculture or added sequentially.

Procedures for sequential addition experiments were developed to study the mechanisms involved in the synergistic and inhibitory interactions observed in forage hemicellulose digestion by rumen bacterial cocultures. One organism was allowed to ferment a forage substrate, the culture tube was sterilized and then inoculated with a second organism. No differences were found in the extent of degradation or utilization between fermentations sterilized by oxidation or heat, and based on ease of handling, heat was used in all subsequent experiments. Studies were conducted with Fibrobacter succinogenes A3c, Ruminococcus flavefaciens B34b and Prevotella ruminicola H2b, singly and in all possible combinations. Results from the sequential addition studies substantiated earlier suggestions that the increase observed in hemicellulose utilization results from initial solubilization of the hemicellulose from the forage by the non-utilizer and subsequent utilization of subsequent utilization of this solubilized polysaccharide by the utilizing, but non-degrading organism.

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