Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes.

The Journal of applied bacteriology Pub Date : 1994-11-01 DOI:10.1111/j.1365-2672.1994.tb04404.x

C Niederhauser, C Höfelein, M Allmann, P Burkhalter, J Lüthy, U Candrian

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引用次数: 33

Abstract

The polymerase chain reaction was used to obtain randomly-amplified polymorphic DNA (RAPD) profiles from Listeria spp. and enterobacteria. Eleven different oligonucleotides were evaluated. Only one, HR4 (19mer), generated reproducible and specific profiles for Listeria spp., while results for enterobacteria were controversial. A total of 57 different Listeria strains were subjected to the RAPD analysis and 27 different profiles were recognized. RAPD typing allowed strains of the same serotype to be distinguished but the same profile was obtained from different serotypes of L. monocytogenes in three cases and in one case two different serotypes of L. innocua yielded the same profile. RAPD-typing with HR4 allowed L. monocytogenes contamination in several food outlets to be traced back to a food processing plant. In additional experiments, the general utility of this RAPD system in typing Yersinia enterocolitica, verotoxigenic Escherichia coli and Salmonella enteritidis was evaluated.

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多态细菌DNA的随机扩增:11种寡核苷酸的评价及其在单核增生李斯特菌污染食品中的应用。

利用聚合酶链反应获得李斯特菌和肠杆菌随机扩增的多态性DNA (RAPD)谱。评估了11种不同的寡核苷酸。只有一种HR4 (19mer)对李斯特菌产生了可重复的特异性谱，而对肠杆菌的结果则存在争议。对57株李斯特菌进行RAPD分析，鉴定出27种不同的菌株谱。RAPD分型可以区分出相同血清型的菌株，但在3例单核增生乳杆菌的不同血清型中获得了相同的菌株谱，在1例不同血清型的innocua乳杆菌中获得了相同的菌株谱。利用HR4进行rapd分型，可以将几个食品店的单核增生乳杆菌污染追溯到一家食品加工厂。在其他实验中，评估了该RAPD系统在小肠结肠炎耶尔森菌、产毒大肠杆菌和肠炎沙门氏菌分型中的一般效用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Journal of applied bacteriology

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