Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures.

The Journal of applied bacteriology Pub Date : 1994-08-01 DOI:10.1111/j.1365-2672.1994.tb03059.x

J E Olsen, D J Brown, D L Baggesen, M Bisgaard

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引用次数: 23

Abstract

Four strains of Salmonella berta and one of Salm. enteritidis were stored as stab cultures in sugar-free agar at 5 degrees, 22 degrees and 30 degrees C and in 15% glycerol at -80 degrees C. The stability of the plasmid profiles in each of the strains was monitored over a period of 2.5 years. Plasmid profiles were stable in all strains stored at -80 degrees C, and only six of 450 colonies examined from strains kept in sugar-free agar at 5 degrees C had lost plasmid molecules. Seventy of 440 colonies from stab cultures that were kept at 22 degrees C, and 71 of 440 colonies at 30 degrees C showed changed plasmid profiles. The total number of plasmids lost increased with time, and occasionally, more than one plasmid molecule was lost in the same strain. The virulence associated plasmid of Salm. enteritidis was remarkably stable as it was maintained in all colonies examined at all temperatures investigated. Likewise, no change in SmaI restriction profile was observed in this plasmid molecule at any temperature.

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五株沙门菌在不同温度刺培养中质粒的稳定性。

四株贝尔塔沙门氏菌和一株沙姆沙门氏菌。在5度，22度和30度的无糖琼脂中以及在-80度的15%甘油中作为刺培养物保存，每个菌株的质粒谱的稳定性在2.5年的时间内被监测。质粒谱在-80℃保存的所有菌株中都是稳定的，在5℃无糖琼脂中保存的450个菌落中只有6个丢失了质粒分子。在22℃和30℃条件下，440个菌落中有71个和70个菌落的质粒谱发生了变化。质粒丢失的总数随着时间的推移而增加，偶尔在同一菌株中丢失多个质粒分子。Salm的毒力相关质粒。肠炎菌是非常稳定的，因为它在所有温度下都保持在所有的菌落中。同样，在任何温度下，该质粒分子中也没有观察到smi限制谱的变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Journal of applied bacteriology

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