K. Nishikawa, Y. Furuta, Toshihiko Yamada, S. Kudo
{"title":"Genetic studies of α-amylase isozymes in wheat VII. Variation in diploid ancestral species and phylogeny of tetraploid wheat","authors":"K. Nishikawa, Y. Furuta, Toshihiko Yamada, S. Kudo","doi":"10.1266/JJG.67.1","DOIUrl":"https://doi.org/10.1266/JJG.67.1","url":null,"abstract":"In order to investigate the phylogeny of polyploid wheat, the genetic variation of α-amylase isozymes in 127 strains of two diploid wheat species and five Aegilops species belonging to section Sitopsis was analyzed. Three types of zymogram were detected in 39 strains of Triticum monococcum and three other different types were detected in another diploid wheat, T. urartu. Two varieties of Ae. speltoides, var. ligustica and var. aucheri (subsection Truncata), were similar to each other and showed wide variation of zymograms. Sixteen strains of Ae. longissima and 13 strains of Ae. sharonensis were each grouped into four types. Ten strains of Ae. bicornis were grouped into three types, each including three or four strains. On the other hand, zymograms from five strains of Ae. searsii, which was formerly treated as a variety of Ae. longissima, were very different from those of Ae. longissima. In contrast to subsection Truncata, the zymogram types were rather simple and similar among species in subsection Emarginata (Ae. longissima, Ae. sharonensis, and Ae.bicornis) except for Ae. searsii. Finally, the phylogeny of tetraploid wheat was discussed on the basis of the results presented here along with those of the previous authors.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"24 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"1992-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76760559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Yoshimura, A. Yoshimura, A. Saito, N. Kishimoto, M. Kawase, M. Yano, M. Nakagahra, T. Ogawa, N. Iwata
{"title":"RFLP analysis of introgressed chromosomal segments in three near-isogenic lines of rice for bacterial blight resistance genes, Xa-1, Xa-3 and Xa-4","authors":"S. Yoshimura, A. Yoshimura, A. Saito, N. Kishimoto, M. Kawase, M. Yano, M. Nakagahra, T. Ogawa, N. Iwata","doi":"10.1266/JJG.67.29","DOIUrl":"https://doi.org/10.1266/JJG.67.29","url":null,"abstract":"Introgressed chromosome segments in three near-isogenic lines (NILs) of rice for bacterial blight resistance genes Xa-1, Xa-3 and Xa-4 with the genetic backgrounds of an IR24 variety were measured by using restriction fragment length polymorphic (RFLP) markers and linkage map. The size of the introgressed segment flanking Xa-1 of a NIL was estimated to be 3.3-11.7 cM of map units. Both NILs for Xa-3 and Xa-4 contained the donor segments at an identical end of linkage group corresponding to chromosome 11. We demonstrated in this study that these NILs were good materials to identify DNA markers tightly linked to these resistance genes and the RFLP markers tagging the resistance genes were useful in genetics and breeding on the disease resistance.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"2 1","pages":"29-37"},"PeriodicalIF":0.0,"publicationDate":"1992-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90139835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic variability and geographical structure in partially selfing populations","authors":"K. Maruyama, H. Tachida","doi":"10.1266/JJG.67.39","DOIUrl":"https://doi.org/10.1266/JJG.67.39","url":null,"abstract":"In order to clarify the effect of selfing on genetic variation in geographically structured populations, Wright's island model is generalized to include; the effects of extinction, mutation, selfing, and migration of pollen and seed. The equilibrium probabilities of identity by descent for two genes are obtained. It is found that selfing causes a decrease of the variation within colonies and an increase of the variation between colonies. Our model partly explains the \"heterozygosity paradox\" but other factors such as selection are necessary to fully account for the paradox.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"12 1","pages":"39-51"},"PeriodicalIF":0.0,"publicationDate":"1992-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74910106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Spatial autocorrelation analysis of allozyme variants within local sites of wild radish population","authors":"T. Tokunaga, O. Ohnishi","doi":"10.1266/JJG.67.209","DOIUrl":"https://doi.org/10.1266/JJG.67.209","url":null,"abstract":"The spatial distribution of allozyme alleles was studied at two local sites of a natural population of wild radish, an inland site in 1988 and 1989 and a beach site in 1988. Randomness of the spatial distribution of genotypes was tested at four isozyme loci in adult plants and fertilizing pollen by spatial autocorrelation statistics. At the inland site, significant aggregation of an identical allele was observed at two loci each in 1988 and 1989 for adult plants. At these loci, aggregation of an allele was observed also for pollen cloud. The aggregation, however, was not stable; the loci which showed significant aggregation differed in two successive seasons. These observations can be interpreted either by limited but moderate pollen dispersal or the great variation in fertility among the adult plants. The latter accidentally causes aggregation of identical alleles. At the beach site, the seed bank was disturbed by tractors for fishing work, and this led to a random distribution of alleles.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"1 1","pages":"209-216"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75327134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inheritance of anthocyanin pigmentation in flower color of chrysanthemum","authors":"K. Hattori","doi":"10.1266/JJG.67.253","DOIUrl":"https://doi.org/10.1266/JJG.67.253","url":null,"abstract":"Inheritance of the flower color in chrysanthemum particularly anthocyanin pigmentation was analyzed by using spectrophotometry. Genetic analysis of the inheritance of anthocyanin pigmentation showed that chrysanthemum cultivars used in this experiment belonged to three groups. In the first group the gene for anthocyanin pigment biosynthesis appeared in the homozygous recessive state. The cultivars belonging to this group had either white or yellow flower and can be useful for testing lines for anthocyanin pigmentation. In the second group, the genes were in the heterozygous condition. While in the third group, the presence of two genes in the heterozygous state with two distinct genes was noted. It was suggested that a chimerical structure be considered in the analysis of the flower color inheritance. The possible role of the gene in the step involving dihydroflavonol to anthocyanin formation in the anthocyanin biosynthesis was also presented.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"72 1","pages":"253-258"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87244885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tohru Suzuki, C. Nakamura, H. Sakagami, N. Mori, I. Panayotov, C. Kaneda
{"title":"Interaction between telocentric nucleolus-organizing chromosomes of two Agropyron species and their cytoplasms in alloplasmic lines of common wheat","authors":"Tohru Suzuki, C. Nakamura, H. Sakagami, N. Mori, I. Panayotov, C. Kaneda","doi":"10.1266/JJG.67.133","DOIUrl":"https://doi.org/10.1266/JJG.67.133","url":null,"abstract":"Alloplasmic lines of common wheat (Triticum aestivum L. cultivars `Penjamo 62' and `Siete Cerros 66') with cytoplasms of two wheatgrass species (Agropyron glaucum and Ag. trichophorum) showed severe growth depression throughout their development and male sterility at maturity. Growth vigor of the alloplasmic lines was influenced more by cytoplasm donors than by nucleus donors, while male sterility was influenced more by nucleus donors. Short-arm telocentric, nucleolus-organizing chromosomes (telosomes) derived from Agropyron caused the restoration of growth vigor and male fertility of the alloplasmic lines in which cytoplasms and telosomes from the same Agropyron species were combined. The telosomes derived from Agropyron species different from the cytoplasm donors could also recover growth vigor and male fertility of the alloplasmic lines. The result indicates the presence of a common mechanism(s) which controls the nucleus-cytoplasmic incompatibility expressed in the alloplasmic lines with the two Agropyron cytoplasms. The Agropyron telosomes showed some dosage effects on growth vigor of the alloplasmic lines and affected fertility when added to the nuclear backgrounds of the two common wheat cultivars.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"37 1","pages":"133-145"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85827363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multiple locations of the rRNA genes in chromosomes of pines, Pinus densiflora and P. thunbergii","authors":"M. Hizume, F. Ishida, M. Murata","doi":"10.1266/JJG.67.389","DOIUrl":"https://doi.org/10.1266/JJG.67.389","url":null,"abstract":"The ribosomal RNA (rRNA) genes were mapped on somatic chromosomes of Japanese red pine (Pinus densiflora) and Japanese black pine (P. thunbergii) by in situ hybridization using biotin-labeled 18S-25S rDNA. Cytochemical detection of the hybridized probe showed many signals in the interphase nuclei and, prophase and metaphase chromosomes in both species. In the metaphase complement of P. densiflora, the signals appeared singly at the interstitial region of seven pairs of the chromosomes. In P. thunbergii, ten strong and two weak signals appeared at the interstitial region of six pairs of the chromosomes. The hybridization sites coincided with the interstitial chromomycin A3-bands or secondary constricted regions.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"112 1","pages":"389-396"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73702576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Size variation of chromomycin A3-band in chromosomes of Douglas fir, Pseudotsuga menziesii","authors":"M. Hizume, M. Akiyama","doi":"10.1266/JJG.67.425","DOIUrl":"https://doi.org/10.1266/JJG.67.425","url":null,"abstract":"Somatic chromosomes of 307 seedlings of Pseudotsuga menziesii (Mirb.) Franco var. menziesii from four native localities in Canada and 203 seedlings of P. menziesii var. glauca (Beissn) Franco from two localities in U.S.A. were stained with guanine-specific chromomycin A3 (CMA). The chromosome complements (2n=26) of the two varieties were commonly composed of ten metacentric, 12 submetacentric and four telocentric chromosomes. Six thick CMA-bands appeared in the interstitial region of a pair of metacentric chromosomes and in the proximal regions of the long arms of two pairs of submetacentric chromosomes. However, the CMA-bands varied in size among the members of the populations. The large CMA-bands of the submetacentric chromosome were observed more frequently in var. glauca than in var. menziesii. Frequency of each sized CMA-band varied within the populations or the varieties. Bright CMA-blocks were associated to nucleoli in the interphase nucleus and the CMA-bands were coincided in location with the secondary constrictions. Ribosomal DNA hybridized in situ to the thick CMA-band regions.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"59 1","pages":"425-435"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85504409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Gravity responsible protein and mRNA related to the survival of rice (Oryza sativa L.) from gravity stress","authors":"Soon-tae Kwon, K. Oono","doi":"10.1266/JJG.67.321","DOIUrl":"https://doi.org/10.1266/JJG.67.321","url":null,"abstract":"Rice (Oryza sativa L. var. Nipponbare) seeds, seedlings and suspension callus were subjected to powerful gravity stress ranging from 150,000 g to 450,000 g. Suspension callus and dehulled seeds showed 32% and 15% survival rates, respectively, after gravity treatment at 450,000 g for 6 hours, whereas all the seedlings died. Seedlings became increasingly sensitive to gravity stress as the growth stage advanced. The suspension callus, which gradually recovered viability during subculture under normal conditions, were used to analyze the protein patterns and in vitro translation products of poly(A)+RNA. Some 3 to 4 newly synthesized proteins and in vitro translation products were identified using two-dimensional electrophoresis, while 5 to 24 proteins disappeared during the treatment, demonstrating that rice suspension callus respond to gravity stress by producing new mRNA and their proteins. In addition, some newly synthesized proteins which resulted from gravity effects were maintained in the surviving cells during continuous growth on solid medium. There was an increase in the survival rate of suspension callus from the strong gravity stress as a result of treatment with 200 g/l sucrose, 10-5 M ABA or 5 g/l NaCl. This suggests that in addition to changes in protein patterns, another protection mechanism associated with some physiological changes is involved in the survival of rice suspension callus after gravity stress.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"1 1","pages":"321-334"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90238353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kanazawa, W. Sakamoto, M. Nakagahra, K. Kadowaki, N. Tsutsumi, S. Tano
{"title":"Distribution and quantitative variation of mitochondrial plasmid-like DNAs in cultivated rice (Oryza sativa L.)","authors":"A. Kanazawa, W. Sakamoto, M. Nakagahra, K. Kadowaki, N. Tsutsumi, S. Tano","doi":"10.1266/JJG.67.309","DOIUrl":"https://doi.org/10.1266/JJG.67.309","url":null,"abstract":"Distribution of four kinds of mitochondrial plasmid-like DNAs, Bl, B2, B3 and B4 was investigated by Southern analysis using 85 accessions of cultivated rice (Oryza sativa L.). The frequencies of these molecules found in cultivars differed from each other. The mitochondrial genome were categorized into nine types according to their presence or absence. They were found mostly in ecospecies Indica, a few in Javanica, and not found in Japonica. The result indicated the polymorphic and monomorphic patterns of the mitochondrial genomic organization within Indica and Japonica cultivars, respectively. Quantitative variation was found among four kinds of mitochondiral plasmid-like DNAs, suggesting that these molecules were unequally distributed or replicated during the process of mitochondrial division.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"7 1","pages":"309-319"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86314816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}