The Japanese Journal of Genetics最新文献_第10页

Fertilization of hetero-specific insect eggs by sperm injection 用精子注射使异源昆虫卵受精

The Japanese Journal of Genetics Pub Date : 1991-01-01 DOI: 10.1266/JJG.66.297

M. Sawa

引用次数: 5

Preferential occurrence of specific R-D chromosome constitutions in stable hexaploid progenies of the hybrid between hexaploid triticale and bread wheat 六倍体小黑麦与面包小麦杂交的稳定六倍体后代中特定R-D染色体结构优先发生

The Japanese Journal of Genetics Pub Date : 1991-01-01 DOI: 10.1266/JJG.66.587

S. Taketa, T. Nakazaki, S. Shigenaga, H. Yamagata

{"title":"Preferential occurrence of specific R-D chromosome constitutions in stable hexaploid progenies of the hybrid between hexaploid triticale and bread wheat","authors":"S. Taketa, T. Nakazaki, S. Shigenaga, H. Yamagata","doi":"10.1266/JJG.66.587","DOIUrl":"https://doi.org/10.1266/JJG.66.587","url":null,"abstract":"Forty-six stable (21II) hexaploid plants were cytologically screened in the F5 generation of a cross between a hexaploid triticale cv. Armadillo (2D/2R substitution type) and a bread wheat cv. Chinese Spring. In order to determine the constitution of R- and D-genome chromosomes of the stable F5 plants, F6 progeny of each F5 plant was analyzed by C-banding. Of the 46 plants, 43 had no translocation, while one was homozygous and two were heterozygous for translocation. Of the theoretically possible 26 = 64 kinds of chromosome constitutions, only 12 kinds were obtained in the stable plants without translocation. They had zero to six pairs of R-genome chromosomes and appeared with different frequencies. Frequently observed chromosome constitutions, which independently originated from many F2 progenitors, had one, four, five or six pairs of R-genome chromosomes. Chromosomes 1R, 3R and 6R were independently replaced by their respective homoeologous D-genome chromosomes. Chromosomes 4R, 5R and 7R always behaved together except in two infrequent chromosome constitutions in which 5R was separated from 4R and 7R. From the information so far reported about the homoeologous relationship between rye and wheat chromosomes, we inferred that the incomplete homoeology of 4R, 5R and 7R to the corresponding homoeologous D-genome chromosomes was responsible for the concurrent presence or absence of these three R-genome chromosomes.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"48 1","pages":"587-596"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89844200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Variation of spontaneous somatic mutation frequency in the stamen hairs of a mutable clone of Tradescantia, KU 20 花蕊花突变无性系ku20雄蕊毛体细胞突变频率的变化

The Japanese Journal of Genetics Pub Date : 1991-01-01 DOI: 10.1266/JJG.66.501

Toshihiko Imai, S. Ichikawa, Marie Sanda-Kamigawara

{"title":"Variation of spontaneous somatic mutation frequency in the stamen hairs of a mutable clone of Tradescantia, KU 20","authors":"Toshihiko Imai, S. Ichikawa, Marie Sanda-Kamigawara","doi":"10.1266/JJG.66.501","DOIUrl":"https://doi.org/10.1266/JJG.66.501","url":null,"abstract":"Variation of spontaneous somatic pink mutation frequency was studied in the stamen hairs of Tradescantia KU 20 clone, a highly mutable blue/pink heterozygote. The spontaneous mutation frequency varied greatly from 4.03±1.21 to 120±7 and from 18.8±3.1 to 110±5 pink mutant events per 103 hairs when the plants were grown outdoors and in the greenhouse, respectively, being generally higher at lower temperature and also in the greenhouse than in outdoors. The spontaneous mutation frequency under controlled environmental conditions also varied from 3.06±0.37 to 40.8±3.1 pink mutant events per 103 hairs, showing a clearer negative correlation with temperature. It was found that the spontaneous mutation frequency under controlled environmental conditions increased when day/night temperature shifts were applied, especially with a 5°C shift than with 3°C shifts. The difference between the highest and the lowest mutation frequencies reached almost 40 times, and this clone was confirmed to be a temperature-sensitive mutable clone. A repair mechanism of DNA damages occurring spontaneously, which is more effective at higher temperature, thus presumably an enzymatic one, is very likely involved in the mutable nature of this clone.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"26 1","pages":"501-511"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74918597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

The heterogeneous composition of mitochondrial DNA in somatic hybrid calli and the relatively simple composition of such DNA in regenerated leaves 体细胞杂交愈伤组织中线粒体DNA组成的异质性和再生叶片中线粒体DNA组成的相对单一

The Japanese Journal of Genetics Pub Date : 1991-01-01 DOI: 10.1266/JJG.66.279

H. Honda, K. Itoh, A. Hirai

{"title":"The heterogeneous composition of mitochondrial DNA in somatic hybrid calli and the relatively simple composition of such DNA in regenerated leaves","authors":"H. Honda, K. Itoh, A. Hirai","doi":"10.1266/JJG.66.279","DOIUrl":"https://doi.org/10.1266/JJG.66.279","url":null,"abstract":"Mitochondrial (mt) and chloroplast (ct) DNA were characterized in somatic hybrids obtained by fusion of protoplasts from two species, Nicotiana glauca and N. langsdorffii. Three and a half months after the fusion, total DNA was extracted from 50-100 mg of callus and of leaf tissue. Southern hybridization was carried out with several probes from organelle DNA. The results revealed that widespread rearrangements of mtDNA had occurred in the majority of hybrid calli. Even subclones derived from the same original hybrid callus generated different patterns of rearrangements. In contrast to our results for calli, we found that leaves contained almost a simple parental-type mtDNA, namely, that of N. langsdorffii in this experiment, and few rearrangements of mtDNA were detected. Data obtained with cob as probe distinctly showed the difference between calli and leaves. These results suggest that there may be a correlation between the organization of mtDNA in hybrids and the ability of hybrids to regenerate, since nuclear genomes of hybrids are known to be stable. Segregation of two kinds of ctDNA was observed more frequently in leaves than in calli. It was also apparent that there was no correlation between species of chloroplasts and species of mitochondria since we found a heterogeneous component of organelles.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"6 1","pages":"279-289"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76056637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Variation of S-alleles and S-glycoproteins in a naturalized population of self-incompatible Brassica campestris L. 自交不亲和油菜归化群体s等位基因和s糖蛋白的变异。

The Japanese Journal of Genetics Pub Date : 1991-01-01 DOI: 10.1266/JJG.66.227

I. Nou, Masao Watanabe, A. Isogai, H. Shiozawa, A. Suzuki, K. Hinata

{"title":"Variation of S-alleles and S-glycoproteins in a naturalized population of self-incompatible Brassica campestris L.","authors":"I. Nou, Masao Watanabe, A. Isogai, H. Shiozawa, A. Suzuki, K. Hinata","doi":"10.1266/JJG.66.227","DOIUrl":"https://doi.org/10.1266/JJG.66.227","url":null,"abstract":"Variation of S-alleles and S-glycoproteins associated with self-incompatibility was studied in a naturalized population of Brassica campestris growing in Oguni-machi, Japan. Of 58 plants collected from the population, 45 were self-incompatible and 8 were self-compatible. Of 32 families investigated on their selfed progenies, 30 families showed segregation fitting to one locus S-allele model of sporophytic self-incompatibility. From cross-pollination experiments between 35 S-homozygotes so far isolated, 16 different S-alleles were identified, and the number of S-alleles involved in this population was estimated to be 20-30. S-glycoproteins corresponding to each S-allele were determined by immunoblotting with polyclonal antibody against S8-glycoprotein. Concanavalin A and Coomassie Blue stainings were also applied to determining corresponding S-glycoproteins, but were not so clear as the antibody cross reaction. It is pointed out that a stigma involves a number of proteins with different pI points, which are cross-reactive with anti-S-glycoprotein-antiserum. Many of these proteins are heritable in correlation with major S-glycoproteins. Since the content of these proteins was variable, we tentatively classified major and minor S-glycoproteins, and assumed that these S-glycoproteins were controlled by S-like DNA sequences with closely linked S-locus. Beside these S-glycoproteins, presence of heterozygote specific proteins was also pointed out, suggesting occurrence of post-transcriptional modification of these proteins. The pI values of major S-glycoproteins ranged from 5.0-9.0 and those at 7.0-9.0 were frequent.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"33 1","pages":"227-239"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83836215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Partial mono-trisomic plants of rice, Oryza sativa L. derived from interchange homozygote, RT2-3b•T65 水稻RT2-3b•T65纯合子的部分单三体植株

The Japanese Journal of Genetics Pub Date : 1991-01-01 DOI: 10.1266/JJG.66.117

Shigetoshi Sato

引用次数: 0

Visualization of replicating bands in plant chromosomes with a monoclonal anti-BrdU antibody method 用单克隆抗brdu抗体方法观察植物染色体复制带

The Japanese Journal of Genetics Pub Date : 1991-01-01 DOI: 10.1266/JJG.66.485

K. Taniguchi, R. Tanaka

引用次数: 7

Physical map of chloroplast DNA of the onion Allium cepa L., showing the location of photosynthesis-related genes 洋葱(Allium cepa L.)叶绿体DNA物理图谱，显示了光合作用相关基因的位置

The Japanese Journal of Genetics Pub Date : 1991-01-01 DOI: 10.1266/JJG.66.421

H. Katayama, T. Sasakuma, Y. Ogihara

引用次数: 14

Survival mechanism of dried calli and regeneration of plants in rice 水稻干愈伤组织存活机制与植株再生

The Japanese Journal of Genetics Pub Date : 1991-01-01 DOI: 10.1266/JJG.66.13

D. Shin, S. Virigool, K. Shinozaki, K. Oono

{"title":"Survival mechanism of dried calli and regeneration of plants in rice","authors":"D. Shin, S. Virigool, K. Shinozaki, K. Oono","doi":"10.1266/JJG.66.13","DOIUrl":"https://doi.org/10.1266/JJG.66.13","url":null,"abstract":"A system for long-term dry preservation of calli of rice (Oryza sativa L.) and a protection mechanism regulating the survival of dried calli were investigated. The highest survival of dried calli and the highest regeneration rate of plantlets were observed in calli which had been pretreated with 10-5 M abscisic acid (ABA) in the presence of 90 g/l of sucrose and were regrown on an R-2 medium. We detected a corresponding accumulation of the transcript RNA of the rab 16A gene (a rice gene induced by ABA and water stress) in dried calli, mature seeds, and calli pretreated with 10-5 M ABA. The levels of this mRNA increased with the increase of the sucrose concentation, indicating that the accumulation of rab 16A mRNA is regulated by ABA at higher concentrations of sucrose and related to the survival of dried calli. Analysis of proteins by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) demonstrated similar protein patterns in fresh and dried calli. However, in the dried calli pretreated with 10-5 M ABA and 90 g/l of sucrose, different protein patterns were found compared to those in a callus dried without the pretreatment, indicating that some specific polypeptides might be synthesized in the pretreated dried callus.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"98 1","pages":"13-25"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77296014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Localization of glutelin gene in rice chromosome by in situ hybridization 水稻染色体谷蛋白基因原位杂交定位

The Japanese Journal of Genetics Pub Date : 1991-01-01 DOI: 10.1266/JJG.66.305

Hiroaki Suzuki, Y. Futsuhara, F. Takaiwa, N. Kurata

引用次数: 9