{"title":"Tracing development of soybean leaf primordia by marking progenitor cells with mutant markers","authors":"T. Itoh, S. Kondo","doi":"10.1266/JJG.67.61","DOIUrl":"https://doi.org/10.1266/JJG.67.61","url":null,"abstract":"Marking cells with genetic marker is a useful way to study plant development. A heterozygous (Y11y11) strain of soybean was exposed to X rays or 14 MeV neutrons before, or 1-7 days after, sowing. Clones of dark-green (due to reversion of the Xantha-like allele y11) or yellow (due to loss of the wild-type allele y11) cells were produced in the first and second compound leaves. Of these clones, none extended beyond the mid-vein. In the first leaf, about half the clones produced by marking before sowing were delimited by the mid-vein but extended beyond the main lateral veins; whereas, about 20% were bounded by small lateral veins, indicative that in the first-leaf primordium of the dormant embryo the mid-vein is already differentiated, but that only about half of the main lateral veins are. After marking on day 7, 99% of the marked clones in the first leaf were bounded by small lateral veins. This and the preceding results indicate that most small lateral veins develop during the first seven days after sowing, together with development of the leaf lamina. Primordia of the 6th or later leaves in dormant seeds exposed to neutrons produced two marked clones that extended over the mid-vein, showing that the mid-vein and lamina are not completely differentiated at this early stage.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"516 1","pages":"61-70"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77107548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Restriction fragment length polymorphism of glutelin from cultivated rice (Oryza sativa L.)","authors":"F. Takaiwa, K. Oono","doi":"10.1266/JJG.66.155","DOIUrl":"https://doi.org/10.1266/JJG.66.155","url":null,"abstract":"Restriction fragment length polymorphism (RFLP) of the glutelin genes was studied within the 29 accessions of cultivated Oryza sativa. The glutelin genes of Japonica type showed monomorphic Southern hybridization pattern, whereas those of the Indica type showed an extensive variation. At least four types of genomic organization of glutelin genes were observed in the cultivated species of Oryza sativa.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"59 1","pages":"155-160"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90717951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diallel analysis of callus growth and plant regeneration in rice seed-callus","authors":"T. Abe, Y. Futsuhara","doi":"10.1266/JJG.66.129","DOIUrl":"https://doi.org/10.1266/JJG.66.129","url":null,"abstract":"Genetic characteristics of callus growth and plant regeneration in rice with seed-derived calli were studied in 6 × 6 diallel crosses using Japonica cultivars. Genetic parameters estimated by Hayman's method showed the high additive gene effects and involvement of two groups of genes for callus growth, which were identified as incomplete dominance. On the other hand, both dominance and additive effects were important for plant regeneration, and further epistatic effects were observed in this character, while the analysis of 5 × 5 subdiallel for plant regeneration showed non-epistatic relation and involvement of 2 groups of genes. Graphical analysis using Vr and Wr for the callus growth showed that Norin 1, Somewake and Daikoku 1 possessed dominant genes which suppress callus growth, while Kuju, Sasanishiki and Murasaki-ine whose callus growth was more vigorous had recessive genes. The frequency distribution of callus growth in F2 generation between Kuju and Somewake showed a segregation which agreed with the expected ratio for a single factor control (3:1). On the other hand, relationship between Vr and Wr in the 5 × 5 subdiallel for plant regeneration showed that Daikoku 1, which had a relatively high capacity for plant regeneration, possessed more dominant genes, while Norin 1 and Kuju, which had lower capacities, possessed recessive genes. The reciprocal F1s between Norin 1 and Somewake showed excellent capacities for plant regeneration (average of reciprocal crosses: 62%). Strategies for genetical improvement of plant regeneration are discussed.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"103 1","pages":"129-140"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84673762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Somatic reversion of a Xantha-like gene in soybean by fast neutrons and X rays","authors":"T. Itoh, S. Kondo","doi":"10.1266/JJG.66.461","DOIUrl":"https://doi.org/10.1266/JJG.66.461","url":null,"abstract":"The variety T219 of Glycine max (soybean) has a wild-type chlorophyll development gene Y11 and its allele y 11. Seeds from autogamous T219 plants produce dark green (Y11Y11), light green (Y 11y11) and yellow (y11 y11) seedlings. Upon irradiation of dry seeds with X rays, the frequency of light-green mosaics on y11 y11 simple leaves was about twice as high as that of dark-green mosaics on Y11y11 simple leaves. For the explanation of the two-fold difference in mutability, we propose that both the light-green and the dark-green mosaics are caused by reversion of y11 to Y11, as the number of target gene y11 per cell in the y 11y11 tissue is twice that in the Y 11y11 tissue. Somatic reversion of the y11 gene was induced, in either y11 y11 or Y11y11 plants by 14 MeV neutrons and by fission neutrons at a rate about 20 times higher than that by X rays, suggesting that the reversions result from deletion mutations. To explain the occurrence of the reversion by deletions, we assume that the y11 gene is a complex gene made of a transposable element inserted at the Y 11 locus and that the reversion resulted from the deletion of the inserted transposon. The phenotype of the y11 gene shares many similarities with those of Xantha genes mapped at several loci in barley and tomato.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"49 1","pages":"461-469"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78863482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Ootaki, A. Miyazaki, J. Fukui, Yuji Kimura, Seiichi Umemura, R. Okazaki, M. Jayaram
{"title":"A high efficient method for introduction of exogenous genes into Phycomyces blakesleeanus","authors":"T. Ootaki, A. Miyazaki, J. Fukui, Yuji Kimura, Seiichi Umemura, R. Okazaki, M. Jayaram","doi":"10.1266/JJG.66.189","DOIUrl":"https://doi.org/10.1266/JJG.66.189","url":null,"abstract":"An efficient method is described for obtaining transformants in Phycomyces by microinjection of plasmid DNA carrying the kanamycin resistance gene from Tn903 into a young sporangium. Approximately 9% of the colonies, germinated from the spores which were developed in the sporangium, were resistant to G418 when a 36 ng sample of the transforming plasmid DNA was injected into a sporangium. The transformation efficiency (transfomants/total colonies) was about 350 times higher than that (transfomants/total protoplasts) obtained with the ordinary protoplast method.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"29 1","pages":"189-195"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89872768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of somatic mutation frequencies in the stamen hairs of one mutable and two stable clones of Tradescantia treated with small doses of gamma rays","authors":"S. Ichikawa, Toshihiko Imai, A. Nakano","doi":"10.1266/JJG.66.513","DOIUrl":"https://doi.org/10.1266/JJG.66.513","url":null,"abstract":"Induced somatic pink mutation frequencies in the stamen hairs of Tradescantia KU 20 clone, a blue/pink heterozygote highly mutable spontaneously at lower temperature, were studied after treating with relatively small doses of 60Co gamma rays (39 to 551 mGy or 3.9 to 55.1 rad), and were compared with those of two stable clones (non-mutable spontaneously), BNL 02 and KU 9, which are also blue/pink heterozygotes. It was found that the gamma-ray-induced mutation frequency in KU 20 clone was comparable (18.8 pink mutant events per 104 hair-cell divisions per Gy) to those in BNL 02 (12.2 and 21.2) and KU 9 (17.4) clones, when the spontaneous mutation frequencies of KU 20 clone were relatively low (at most about 5.7 and 2.3 times of BNL 02 and KU 9 clones, respectively). However, when the spontaneous mutation frequencies of KU 20 clone were much higher (up to about 65 and 27 times of BNL 02 and KU 9 clones, respectively), induced mutation frequency was significantly higher in KU 20 clone (58.8 pink mutant events per 104 hair-cell divisions per Gy) than in BNL 02 and KU 9 clones. The extent of increase in the gamma-ray-induced mutation frequency in the latter case was nevertheless very much less than the increase in the spontaneous mutation frequency, suggesting different mechanisms of initiation and repair of radiation-induced and spontaneous mutations.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"1 1","pages":"513-525"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88956717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"UV-B injury in rice plants: A genetic study","authors":"H. Suge, Tadashi Sato, T. Kumagai","doi":"10.1266/JJG.66.347","DOIUrl":"https://doi.org/10.1266/JJG.66.347","url":null,"abstract":"Application of an inhibitor of gibberellin biosynthesis alleviated the UV-B injury in rice seedlings when tested for chlorophyll content per unit fresh and dry weight. A genetic study was conducted by crossing Tan-ginbozu, a gibberellin-deficient mutant, against two floating rice cultivars that presumably contain large amounts of endogenous gibberellins. The heritabilities of plant height, and chlorophyll content per unit fresh and dry weight were high in the F2 plants in non UV-B irradiated control populations. When those plants were treated with UV-B irradiation, the F2 population tended to shift to the lower range in the chlorophyll content per unit weight, and the value of heritability fluctuated depending on the cross and characters measured. Selection of plants that are highly resistant to UV-B irradiation can be made possible by finding the range of UV-B energy that increases the difference between sensitive and resistant, while also increasing the value of the heritability.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"28 1","pages":"347-361"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90518336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survey of endogenous gibberellins in a barley mutant showing abnormal response to gravity","authors":"I. Turkan, H. Suge","doi":"10.1266/JJG.66.41","DOIUrl":"https://doi.org/10.1266/JJG.66.41","url":null,"abstract":"Contents of endogenous gibberellins were surveyed in a barley mutant showing abnormal response to gravity in comparison with the original normal line. The contents were assayed by using gibberellin-sensitive dwarf rice strains Tanginbozu and Waito-C. Gibberellin activities were found to be greatly reduced in the mutant line throughout the growth period, and in different plant parts, i, e., leaves, stems and heads. After heading time, gibberellin activities obtained by the Waito-C assay was especially reduced in the mutant line. These results suggest that biosynthesis, especially the ability in the site of 3β-hydroxylation, is greatly reduced in the mutant line.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"45 1","pages":"41-48"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90237302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Ueda, R. Satō, M. Iwata, A. Komaru, J. Kobayashi
{"title":"The viable 3.5n trouts produced between diploid females and allotriploid males.","authors":"T. Ueda, R. Satō, M. Iwata, A. Komaru, J. Kobayashi","doi":"10.1266/JJG.66.71","DOIUrl":"https://doi.org/10.1266/JJG.66.71","url":null,"abstract":"Eyed embryos and fingerlings of trouts between diploid females and allotriploid males were obtained. There were two groups, namely, one group with the chromosome number of 79-83 (group I), and another with that of 110-112 (group II). Judging from numbers and constitutions of the chromosomes, it is presumed that the individuals in group I were 2.5n and those in group II were 3.5n. All fingerlings were 3.5n, and 2 fingerlings of them were still surviving as of September, 1990, about 7 months after the hatching.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"35 1","pages":"71-75"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74731693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hybrid progenies of the cross, Brassica campestris×B. oleracea. IV : Crossability of F2, B1 and hybrid plants, and their progenies","authors":"N. Inomata","doi":"10.1266/JJG.66.449","DOIUrl":"https://doi.org/10.1266/JJG.66.449","url":null,"abstract":"Crossability was examined in the F2, B1 and hybrid plants, and the somatic chromosomes of their progenies were investigated. The F2 and B1 plants were obtained in the self- and open pollinations, and in the F1 hybrids backcrossed to both parents, respectively. The hybrid plants were obtained in the cross between F1 hybrids and diploid Brassica oleracea, and between F1 hybrids and B. napus. All F1 hybrids were produced by ovary culture in vitro in the cross between B. campestris and B. oleracea, and between B. campestris and autotetraploid B. oleracea. The plants examined were classified into three types. The first type had 29 chromosomes in root tip cells, and the second had 38 chromosomes. The third type of plants was the plants having chromosomes other than 29 or 38 chromosomes. Many different types of aneuploids were obtained in open pollination of the F2, B1 and hybrid plants, in the progenies of the F2, B1 and hybrid plants crossed to B. campestris, and B. napus. It was suggested that the different types of normal egg cell with one by one or little by little chromosome addition might be produced, and that some specific chromosome or chromosome group might increase or decrease the crossability in aneuploid production. It might be considered that there were genes related to fertility. It might be possible that the genes of B. campestris and B. oleracea could exchange reciprocally. Further selection had to be done for the production of a new type crop of B. napus which had been reported in a previous paper.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"23 1","pages":"449-460"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87389295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}