一种外源基因导入黑藻的高效方法

T. Ootaki, A. Miyazaki, J. Fukui, Yuji Kimura, Seiichi Umemura, R. Okazaki, M. Jayaram
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引用次数: 11

摘要

本文描述了一种将携带Tn903卡那霉素抗性基因的质粒DNA微量注射到芽孢囊中获得植菌转化子的有效方法。将36ng的转化质粒DNA注入孢子囊后,孢子囊中萌发的孢子中约有9%的菌落对G418具有抗性。转化效率(转化菌数/总菌落数)是普通原生质体法(转化菌数/总原生质体数)的350倍左右。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A high efficient method for introduction of exogenous genes into Phycomyces blakesleeanus
An efficient method is described for obtaining transformants in Phycomyces by microinjection of plasmid DNA carrying the kanamycin resistance gene from Tn903 into a young sporangium. Approximately 9% of the colonies, germinated from the spores which were developed in the sporangium, were resistant to G418 when a 36 ng sample of the transforming plasmid DNA was injected into a sporangium. The transformation efficiency (transfomants/total colonies) was about 350 times higher than that (transfomants/total protoplasts) obtained with the ordinary protoplast method.
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