Variation of spontaneous somatic mutation frequency in the stamen hairs of a mutable clone of Tradescantia, KU 20

Toshihiko Imai, S. Ichikawa, Marie Sanda-Kamigawara
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引用次数: 12

Abstract

Variation of spontaneous somatic pink mutation frequency was studied in the stamen hairs of Tradescantia KU 20 clone, a highly mutable blue/pink heterozygote. The spontaneous mutation frequency varied greatly from 4.03±1.21 to 120±7 and from 18.8±3.1 to 110±5 pink mutant events per 103 hairs when the plants were grown outdoors and in the greenhouse, respectively, being generally higher at lower temperature and also in the greenhouse than in outdoors. The spontaneous mutation frequency under controlled environmental conditions also varied from 3.06±0.37 to 40.8±3.1 pink mutant events per 103 hairs, showing a clearer negative correlation with temperature. It was found that the spontaneous mutation frequency under controlled environmental conditions increased when day/night temperature shifts were applied, especially with a 5°C shift than with 3°C shifts. The difference between the highest and the lowest mutation frequencies reached almost 40 times, and this clone was confirmed to be a temperature-sensitive mutable clone. A repair mechanism of DNA damages occurring spontaneously, which is more effective at higher temperature, thus presumably an enzymatic one, is very likely involved in the mutable nature of this clone.
花蕊花突变无性系ku20雄蕊毛体细胞突变频率的变化
以高变异蓝粉杂合子Tradescantia KU 20为材料,对其雄蕊自发体细胞粉红突变频率进行了研究。在室外和温室中,每103根毛羽的粉红突变次数分别为4.03±1.21 ~ 120±7次和18.8±3.1 ~ 110±5次,在低温和温室中均高于室外。可控环境条件下的自发突变频率为3.06±0.37 ~ 40.8±3.1次/ 103根,与温度呈明显负相关。研究发现,在受控环境条件下,当昼夜温差变化时,自发突变频率增加,特别是当温度变化5°C时,突变频率高于3°C时。最高和最低突变频率之间的差异达到近40倍,该克隆被证实是温度敏感的可变克隆。一种自发发生的DNA损伤修复机制,在更高的温度下更有效,因此可能是一种酶的修复机制,很可能与这种克隆的易变性有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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