Visualization of replicating bands in plant chromosomes with a monoclonal anti-BrdU antibody method

Abstract

In order to detect DNA replication in chromosomes of four plant species, Allium fistulosum (2n=16), Crepis capillaris (2n=6), Haplopappus gracilis (2n=4) and Chrysanthemum coronarium var. spatiosum (2n=18), an immunological technique using monoclonal anti-BrdU antibody was utilized. In this technique, HCl was used for denaturation and avidin-biotin complex for the immunological process. The replicating bands which appeared with this technique were of unusually high resolution.