{"title":"孟氏伪杉树染色体中染色体霉素a3带的大小变化","authors":"M. Hizume, M. Akiyama","doi":"10.1266/JJG.67.425","DOIUrl":null,"url":null,"abstract":"Somatic chromosomes of 307 seedlings of Pseudotsuga menziesii (Mirb.) Franco var. menziesii from four native localities in Canada and 203 seedlings of P. menziesii var. glauca (Beissn) Franco from two localities in U.S.A. were stained with guanine-specific chromomycin A3 (CMA). The chromosome complements (2n=26) of the two varieties were commonly composed of ten metacentric, 12 submetacentric and four telocentric chromosomes. Six thick CMA-bands appeared in the interstitial region of a pair of metacentric chromosomes and in the proximal regions of the long arms of two pairs of submetacentric chromosomes. However, the CMA-bands varied in size among the members of the populations. The large CMA-bands of the submetacentric chromosome were observed more frequently in var. glauca than in var. menziesii. Frequency of each sized CMA-band varied within the populations or the varieties. Bright CMA-blocks were associated to nucleoli in the interphase nucleus and the CMA-bands were coincided in location with the secondary constrictions. Ribosomal DNA hybridized in situ to the thick CMA-band regions.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"59 1","pages":"425-435"},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"15","resultStr":"{\"title\":\"Size variation of chromomycin A3-band in chromosomes of Douglas fir, Pseudotsuga menziesii\",\"authors\":\"M. Hizume, M. Akiyama\",\"doi\":\"10.1266/JJG.67.425\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Somatic chromosomes of 307 seedlings of Pseudotsuga menziesii (Mirb.) Franco var. menziesii from four native localities in Canada and 203 seedlings of P. menziesii var. glauca (Beissn) Franco from two localities in U.S.A. were stained with guanine-specific chromomycin A3 (CMA). The chromosome complements (2n=26) of the two varieties were commonly composed of ten metacentric, 12 submetacentric and four telocentric chromosomes. Six thick CMA-bands appeared in the interstitial region of a pair of metacentric chromosomes and in the proximal regions of the long arms of two pairs of submetacentric chromosomes. However, the CMA-bands varied in size among the members of the populations. The large CMA-bands of the submetacentric chromosome were observed more frequently in var. glauca than in var. menziesii. Frequency of each sized CMA-band varied within the populations or the varieties. Bright CMA-blocks were associated to nucleoli in the interphase nucleus and the CMA-bands were coincided in location with the secondary constrictions. Ribosomal DNA hybridized in situ to the thick CMA-band regions.\",\"PeriodicalId\":22578,\"journal\":{\"name\":\"The Japanese Journal of Genetics\",\"volume\":\"59 1\",\"pages\":\"425-435\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"15\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Japanese Journal of Genetics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1266/JJG.67.425\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Japanese Journal of Genetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1266/JJG.67.425","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15
摘要
307株孟氏伪杉苗的体细胞染色体分析用鸟苷特异性色霉素A3 (CMA)对来自加拿大4个地方的门齐弧菌(Franco var. menziesii)和来自美国2个地方的门齐弧菌(P. menziesii var. glauca (Beissn) Franco) 203株幼苗进行了染色。两个品种的染色体互补体(2n=26)通常由10条常著心染色体、12条亚常著心染色体和4条远著心染色体组成。6条较厚的cma条带出现在一对稳心染色体的间隙区和两对亚稳心染色体的长臂近端。然而,种群成员的cma波段大小不同。在变种青光眼中观察到的大cma染色体带比变种孟氏型更常见。各cma波段的频率在种群或品种中存在差异。明亮的cma条带与间期核核仁相关,cma条带与二次缩窄的位置一致。核糖体DNA原位杂交到厚cma带区域。
Size variation of chromomycin A3-band in chromosomes of Douglas fir, Pseudotsuga menziesii
Somatic chromosomes of 307 seedlings of Pseudotsuga menziesii (Mirb.) Franco var. menziesii from four native localities in Canada and 203 seedlings of P. menziesii var. glauca (Beissn) Franco from two localities in U.S.A. were stained with guanine-specific chromomycin A3 (CMA). The chromosome complements (2n=26) of the two varieties were commonly composed of ten metacentric, 12 submetacentric and four telocentric chromosomes. Six thick CMA-bands appeared in the interstitial region of a pair of metacentric chromosomes and in the proximal regions of the long arms of two pairs of submetacentric chromosomes. However, the CMA-bands varied in size among the members of the populations. The large CMA-bands of the submetacentric chromosome were observed more frequently in var. glauca than in var. menziesii. Frequency of each sized CMA-band varied within the populations or the varieties. Bright CMA-blocks were associated to nucleoli in the interphase nucleus and the CMA-bands were coincided in location with the secondary constrictions. Ribosomal DNA hybridized in situ to the thick CMA-band regions.