The International journal of biochemistry最新文献_第2页

Immunological relationship among rat erythrocyte membrane sialoglycoproteins and with human glycophorins. 大鼠红细胞膜唾液糖蛋白与人糖蛋白的免疫学关系。

The International journal of biochemistry Pub Date : 1993-07-01 DOI: 10.1016/0020-711x(93)90114-t

A Herráez, A Díaz, J Luque, J C Díez

引用次数: 2

Identification of alpha 2-adrenoceptors and of non-adrenergic idazoxan binding sites in pancreatic islets from young and adult hamsters. 幼鼠和成年仓鼠胰岛α 2-肾上腺素受体和非肾上腺素能性咪唑嗪结合位点的鉴定。

The International journal of biochemistry Pub Date : 1993-07-01 DOI: 10.1016/0020-711x(93)90124-w

C Lacombe, V Viallard, H Paris

{"title":"Identification of alpha 2-adrenoceptors and of non-adrenergic idazoxan binding sites in pancreatic islets from young and adult hamsters.","authors":"C Lacombe, V Viallard, H Paris","doi":"10.1016/0020-711x(93)90124-w","DOIUrl":"https://doi.org/10.1016/0020-711x(93)90124-w","url":null,"abstract":"1. The current study was undertaken to investigate the characteristics of alpha 2-adrenoceptors and to search for the presence of NAIBS in hamster pancreatic islets. 2. Pancreatic islets were isolated from young (6-7 weeks) and adult (14-15 weeks) animals. 3. The identification of alpha 2-adrenoceptors using [3H]RX821002 indicated that adults exhibited higher number of alpha 2-adrenoceptors than the young animals (194 +/- 20 vs 105 +/- 16 fmol/mg protein) while the Kd value was unchanged. 4. Glucose-evoked insulin release was completely inhibited by the alpha 2-agonist clonidine (0.1 microM) whatever the age of the animals. Agonist inhibition curves showed the following rank order of potency: clonidine > UK14304 > adrenaline. 5. Blockade of UK14304-elicited inhibition by various antagonists indicated that yohimbine has a low affinity for the receptor supporting the conclusion that the receptor is of the alpha 2-D subtype. 6. Binding experiments with [3H]idazoxan under conditions allowing to discriminate between alpha 2-adrenoceptors and NAIBS showed that hamster pancreatic islets express a high number of NAIBS. The density of NAIBS was similar in young and adult hamsters (1550 +/- 245 and 1342 +/- 332 fmol/mg protein).","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(93)90124-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19096821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Characterization of the ferredoxin gene transcripts in bovine liver and brain. 牛肝脏和脑组织铁氧还蛋白基因转录物的研究。

The International journal of biochemistry Pub Date : 1993-07-01 DOI: 10.1016/0020-711x(93)90122-u

Y Matsuo, A Furukawa, Y Tsuneoka, J Ono, Y Ichikawa

{"title":"Characterization of the ferredoxin gene transcripts in bovine liver and brain.","authors":"Y Matsuo, A Furukawa, Y Tsuneoka, J Ono, Y Ichikawa","doi":"10.1016/0020-711x(93)90122-u","DOIUrl":"https://doi.org/10.1016/0020-711x(93)90122-u","url":null,"abstract":"1. To study the expression of a ferredoxin gene in extra-adrenocortical tissues, the amounts and structures of the ferredoxin gene transcripts in bovine liver and brain were studied and compared to those of that in adrenocortex. 2. The sizes and amounts of the ferredoxin mRNAs were analyzed by means of Northern blotting, RNA slot blotting and RT-PCR. 3. The nucleotide sequences were determined for 39 ferredoxin cDNA clones from bovine liver. 4. The results indicated that the sizes of the ferredoxin mRNAs in liver and brain were the same as that in adrenocortex, however, their amounts were approx 1/30th and less than the latter, respectively. 5. Although the multiple forms of ferredoxin cDNA, differing in the poly(A) addition sites, were also found in liver, a minor form of ferredoxin cDNA, produced through alternative promoter usage and splicing, could not be detected in liver. 6. The nucleotide sequences of all hepato-ferredoxin cDNA clones obtained were identical to that of a major type of adreno-ferredoxin. 7. These results showed that the expression level of the ferredoxin gene in different tissues was controlled by the amount of mRNA.","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(93)90122-u","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19352309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Interaction of serum albumin with the Fe(III)-citrate complex. 血清白蛋白与铁(III)-柠檬酸盐复合物的相互作用。

The International journal of biochemistry Pub Date : 1993-07-01 DOI: 10.1016/0020-711x(93)90115-u

R A Løvstad

引用次数: 17

Thyroxine type II 5'-deiodinase activity in pineal and Harderian gland is enhanced by hypothyroidism but is independent of serum thyroxine concentrations during hyperthyroidism. 松果体和哈德氏腺的甲状腺素ⅱ型5′-去碘酶活性在甲状腺功能减退时增强，但在甲状腺功能亢进时与血清甲状腺素浓度无关。

The International journal of biochemistry Pub Date : 1993-07-01 DOI: 10.1016/0020-711x(93)90119-y

C Osuna, J M Orta, A Rubio, P Molinero, J M Guerrero

{"title":"Thyroxine type II 5'-deiodinase activity in pineal and Harderian gland is enhanced by hypothyroidism but is independent of serum thyroxine concentrations during hyperthyroidism.","authors":"C Osuna, J M Orta, A Rubio, P Molinero, J M Guerrero","doi":"10.1016/0020-711x(93)90119-y","DOIUrl":"https://doi.org/10.1016/0020-711x(93)90119-y","url":null,"abstract":"1. This paper studies the effect of thyroid status on 5'-D activity in pineal gland, Harderian gland, brown adipose tissue (BAT), pituitary gland, brain frontal cortex (BFC), and cerebellum. 2. Hypothyroidism clearly increased diurnal 5'-D activity in Harderian gland, BAT, pituitary gland, BFC, and cerebellum. In pineal gland, diurnal values of 5'-D activity were not affected by hypothyroidism. 3. Hypothyroidism in adult rats clearly enhanced nocturnal increase of 5'-D activity in pineal and Harderian gland. Congenital hypothyroidism also enhanced the nocturnal increase of 5'-D activity in pineal gland. 4. Hyperthyroidism inhibited 5'-D activity in pituitary gland, BFC, and cerebellum. A small inhibition, although significant, was found in BAT. 5. In pineal and Harderian gland, hyperthyroidism did not inhibit either the basal diurnal values of the enzyme or the nocturnal increase of its activity. 6. Results suggest that, in tissues where 5'D-activity is regulated by adrenergic mechanisms, mostly pineal gland and Harderian gland, the enzyme activity is independent of serum T4 concentrations during hyperthyroidism.","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(93)90119-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19352308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A comparison of DNA polymerase alpha from untransformed and SV40-transformed human fibroblasts. 未转化和sv40转化的人成纤维细胞DNA聚合酶α的比较。

The International journal of biochemistry Pub Date : 1993-07-01 DOI: 10.1016/0020-711x(93)90121-t

V K Srivastava, M D Schroeder, S M Miller, D L Busbee

引用次数: 0

Spectrofluorometric studies of nile red treated native and oxidized low density lipoprotein. 尼罗河红处理的天然和氧化低密度脂蛋白的荧光光谱研究。

The International journal of biochemistry Pub Date : 1993-07-01 DOI: 10.1016/0020-711x(93)90111-q

P Greenspan, P Lou

引用次数: 11

Characterization of calcyclin fragments obtained by CNBr-cleavage. 通过cnbr切割获得的钙调蛋白片段的表征。

The International journal of biochemistry Pub Date : 1993-07-01 DOI: 10.1016/0020-711x(93)90113-s

U Wojda, J Kuźnicki

引用次数: 4

Identification of annexin II, annexin VI and glyceraldehyde-3-phosphate dehydrogenase as calcyclin-binding proteins in bovine heart. 膜联蛋白II、膜联蛋白VI和甘油醛-3-磷酸脱氢酶作为牛心脏钙环素结合蛋白的鉴定。

The International journal of biochemistry Pub Date : 1993-07-01 DOI: 10.1016/0020-711x(93)90116-v

F Y Zeng, V Gerke, H J Gabius

{"title":"Identification of annexin II, annexin VI and glyceraldehyde-3-phosphate dehydrogenase as calcyclin-binding proteins in bovine heart.","authors":"F Y Zeng, V Gerke, H J Gabius","doi":"10.1016/0020-711x(93)90116-v","DOIUrl":"https://doi.org/10.1016/0020-711x(93)90116-v","url":null,"abstract":"1. Matrix-immobilized calcyclin as affinity ligand in chromatography led to purification of three protein bands at 68, 36 and 35 kDa from bovine heart that required Ca2+ for binding. 2. Polyacrylamide-immobilized phosphatidylserine separated this fraction into a phospholipid-binding part (68 kDa, 35 kDa), also attaching to phospholipid vesicles even in the presence of calcyclin, and a flow-through part, constituting approx 30% of the total fraction (36 kDa). 3. Enzyme assays and electrophoretic mobility showed an at least close relationship of the 36 kDa band to glyceraldehyde-3-phosphate dehydrogenase. Interaction between enzyme and calcyclin in a solid-phase assay was inhibited by sialoglycoproteins and depended strongly on the integrity of carboxyl and hydrophobic groups of the enzyme. The interaction between the two proteins had a KD value of 110 nM. 4. Application of annexin-specific antibodies revealed an immunological relationship of the 35 and 68 kDa calcyclin-binding proteins to members of the annexin family, namely to annexin II (35 kDa) and annexin VI (68 kDa). The N-terminal amino acid sequence of a cleavage peptide of the 68 kDa protein was identical to a sequence stretch in human annexin VI, corroborating this evidence.","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(93)90116-v","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19352305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 84

Post-translational chemical modifications of proteins--III. Current developments in analytical procedures of identification and quantitation of post-translational chemically modified amino acid(s) and its derivatives. 蛋白质翻译后的化学修饰——III。翻译后化学修饰氨基酸及其衍生物的鉴定和定量分析方法的最新进展。

The International journal of biochemistry Pub Date : 1993-07-01 DOI: 10.1016/0020-711x(93)90108-q

K K Han, A Martinage

{"title":"Post-translational chemical modifications of proteins--III. Current developments in analytical procedures of identification and quantitation of post-translational chemically modified amino acid(s) and its derivatives.","authors":"K K Han, A Martinage","doi":"10.1016/0020-711x(93)90108-q","DOIUrl":"https://doi.org/10.1016/0020-711x(93)90108-q","url":null,"abstract":"1. The Chemical modifications of amino acids and their derivatives are mainly due to different post-translational enzymatic reactions. 2. The enzymatic reactions resulting in amino acids such as acetylation-, formylation, methylation-phosphorylation-, sulfation-, hydroxylation, ADP ribosylation-, carboxylation-, amidation-, adenylylation-, glycosylation-, ubiquitination-, prenylation and acylation are listed and analytical methods are reported and extensively reviewed. 3. The post-translationally modified cross-linking molecules after maturations such as desmosines, allo-desmosine, hydroxy-, lysylpyridinoline, 3-hydroxypyridinium derivatives, cyclopentenosine recently found in matured elastin, and in collagen, and pulcherosine a novel tyrosine-derived found in fertilization envelope of Sea Urchin embryo, di-tyrosine in resilin, gamma-glutamyl-lysine isopeptide cross-linking molecule etc. are listed and both physico-chemical and analytical methods are extensively reviewed and discussed. 4. Other consequences of post-translational modifications encountered in the analytical procedure such as N-terminal step-wise Edman degradation of glycosylated site(s), phosphorylated-site(s) and or sulfated-site(s) were also reported by us.","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(93)90108-q","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19352311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19