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The International journal of biochemistry最新文献

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Salt inhibition of nuclear histone acetyltransferase from calf thymus. 盐对小牛胸腺核组蛋白乙酰转移酶的抑制作用。
The International journal of biochemistry Pub Date : 1985-01-01
L J Wong, W F Patton
{"title":"Salt inhibition of nuclear histone acetyltransferase from calf thymus.","authors":"L J Wong,&nbsp;W F Patton","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nuclear histone acetyltransferase isolated from calf thymus was found to be inhibited by numerous salts at millimolar concentrations. Salts made up of monovalent ions caused 50% decrease in enzymatic activity at an average concentration of 51 +/- 14 mM while the same degree of inhibition was achieved by divalent salts at 15 +/- 5 mM. At the same ionic strength in the range from 5 to 70 mM, the divalent salts were 14-31% more inhibitory than the salts of monovalent ions. Kinetic analysis showed that NaCl and (NH4)2SO4 inhibited the enzyme competitively against both acetyl-CoA and histones. The inhibition constants for NaCl against acetyl-CoA and histones are respectively 30 and 34 mM. That for (NH4)2SO4 are 8 and 12 mM respectively.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15108802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Citrate determination with aconitase immobilized on solid support. 固载乌头酸酶测定柠檬酸。
The International journal of biochemistry Pub Date : 1985-01-01
E Cortes, S Viniegra, M S Aguilar, J Gallar
{"title":"Citrate determination with aconitase immobilized on solid support.","authors":"E Cortes,&nbsp;S Viniegra,&nbsp;M S Aguilar,&nbsp;J Gallar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pig heart aconitase has been immobilized on Enzacryl AA solid support and its kinetic behaviours were studied by using a stirred bath reactor with continuous recycling. In this reactor, the best flow rate has been determined to eliminate diffusional problems. Kinetics constants, thermic stability and pH variations have been compared between the soluble and immobilized aconitase for determination of enzyme-Enzacryl AA effectivity. Stability of the soluble and immobilized aconitase was also studied after repeated use and long-time storage. While the soluble form loses its activity after 24 hr storage, the immobilized form preserves its full activity after repeated usage and long-lasting storage. Finally, an easily measurable parameter has been found to quantitate citrate. The maximum increase of absorbance, is proportional to citrate concentration in a range between 0.2 and 3.2 mM. In conclusion, these results show that the immobilized aconitase system can be used for the determination of citrate with reproductility and great sensitivity. In addition to the simplicity of the assay, great economy in enzyme consumption has been demonstrated, in contrast to the traditional methods of quantitative citrate analysis.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15108804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intramembrane fluidity increases when the cyclohexyl ring of retinal binds to rod outer-segment membranes. 当视网膜环己基环与杆外节膜结合时，膜内流动性增加。
The International journal of biochemistry Pub Date : 1985-01-01
D L Vessell
{"title":"Intramembrane fluidity increases when the cyclohexyl ring of retinal binds to rod outer-segment membranes.","authors":"D L Vessell","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The spin-label 5-4',4'-dimethyloxazolidinyl-N-oxy stearate (5-doxyl-stearate) exists in a viscous environment when it is incorporated into bleached rod outer segment membranes (ROSM). Addition of the cyclohexyl ring moiety of retinal to ROSM produced a large increase in intramembrane fluidity. A decrease in both the order parameter S (from 0.5 to 0.14) and the rotational correlation time (from 4.0 to 2.0 nsec) gives an estimate of the magnitude of change. Other ring analogs produced similar fluidity changes. The extent of their action depended on their structural similarity to the cyclohexyl of retinal. The pH range in which the cyclohexyl ring produced the increase in ROSM fluidity was 6.8-9.5.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15108803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Porphyrins and porphyrias: ethiopathogenesis, clinics and treatment. (Proceedings of the First Internatinal Argentine Meeting on Porphyrins and Porphyrias held at the University of Buenos Aires, Argentina, 28 October-1 November, 1979). 卟啉和卟啉症:埃塞俄比亚发病、诊所和治疗。(1979年10月28日至11月1日在阿根廷布宜诺斯艾利斯大学举行的第一次关于卟啉和卟啉症的阿根廷国际会议记录)。
The International journal of biochemistry Pub Date : 1980-01-01
{"title":"Porphyrins and porphyrias: ethiopathogenesis, clinics and treatment. (Proceedings of the First Internatinal Argentine Meeting on Porphyrins and Porphyrias held at the University of Buenos Aires, Argentina, 28 October-1 November, 1979).","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17175058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Measurement of metabolism during renal work. 肾脏工作时代谢的测量。
The International journal of biochemistry Pub Date : 1980-01-01
P Deetjen
{"title":"Measurement of metabolism during renal work.","authors":"P Deetjen","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18409421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biochemical aspects of renal function (proceedings of a symposium held in honour of Professor Sir Hans A. Krebs, Oxford, England, 16-19 September, 1979). 肾功能的生化方面(1979年9月16-19日，英国牛津，为纪念Hans a . Krebs教授而举行的研讨会论文集)。
The International journal of biochemistry Pub Date : 1980-01-01
{"title":"Biochemical aspects of renal function (proceedings of a symposium held in honour of Professor Sir Hans A. Krebs, Oxford, England, 16-19 September, 1979).","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18409529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tissue culture of normal rat glomeruli. Basement membrane biosynthesis by homogeneous epithelial and mesangial cell lines. 正常大鼠肾小球组织培养。均质上皮和系膜细胞系的基底膜生物合成。
The International journal of biochemistry Pub Date : 1980-01-01 DOI: 10.1016/B978-0-08-025517-0.50045-X
J. Foidart, C. Dubois, J. Foidart, C. Dechenne, P. Mahieu
{"title":"Tissue culture of normal rat glomeruli. Basement membrane biosynthesis by homogeneous epithelial and mesangial cell lines.","authors":"J. Foidart, C. Dubois, J. Foidart, C. Dechenne, P. Mahieu","doi":"10.1016/B978-0-08-025517-0.50045-X","DOIUrl":"https://doi.org/10.1016/B978-0-08-025517-0.50045-X","url":null,"abstract":"","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82769814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Effect of temperature on the kinetics of the yeast AMP deaminase. 温度对酵母菌AMP脱氨酶动力学的影响。
The International journal of biochemistry Pub Date : 1900-01-01 DOI: 10.1016/0020-711X(86)90111-4
Yoshino Masataka, Murakami Keiko
{"title":"Effect of temperature on the kinetics of the yeast AMP deaminase.","authors":"Yoshino Masataka, Murakami Keiko","doi":"10.1016/0020-711X(86)90111-4","DOIUrl":"https://doi.org/10.1016/0020-711X(86)90111-4","url":null,"abstract":"","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86686623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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