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The Italian journal of biochemistry最新文献

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Interplay between wrn and the checkpoint in s-phase. 在s阶段wrn和检查点之间的相互作用。
The Italian journal of biochemistry Pub Date : 2007-06-01
Pietro Pichierri
{"title":"Interplay between wrn and the checkpoint in s-phase.","authors":"Pietro Pichierri","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Stability of the genome is crucial for survival and faithful transmission of the genetic blueprint to progenitors. During DNA replication chromosome integrity can be challenged by a variety of exogenous and endogenous damaging agents and by the process of duplication itself. Thus, eukaryotic cells have evolved a sophisticated response called replication checkpoint supervising the accurate and complete genome replication. The replication checkpoint response bridges together replication, repair and cell cycle proteins in a coordinated network having the ATR kinase as culprit. ATR-mediated phosphorylation events control that stalled replication forks are properly sensed and stabilised, cell cycle progression halted and replication eventually recovered. In the recent years, the Werner syndrome protein (WRN) emerged as a central actor of the replication checkpoint being instrumental for correct recovery from arrested replication and a substrate of ATR. In this review, how WRN and the replication checkpoint could cross-talk and contribute to faithful recovery of stalled replication forks will be discussed.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26909569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification, kinetic properties and physicochemical characterization of a novel acid phosphatase (AP) from germinating peanut (Arachis hypogaea) seed. 花生发芽种子中一种新型酸性磷酸酶(AP)的纯化、动力学性质和理化性质。
The Italian journal of biochemistry Pub Date : 2007-06-01
Jean Tia Gonnety, Sébastien Niamké, Betty Meuwiah Faulet, Eugène Jean-Parfait N'guessan Kouadio, Lucien Patrice Kouamé
{"title":"Purification, kinetic properties and physicochemical characterization of a novel acid phosphatase (AP) from germinating peanut (Arachis hypogaea) seed.","authors":"Jean Tia Gonnety,&nbsp;Sébastien Niamké,&nbsp;Betty Meuwiah Faulet,&nbsp;Eugène Jean-Parfait N'guessan Kouadio,&nbsp;Lucien Patrice Kouamé","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acid phosphatase activity was detected in peanut (Arachis hypogaea) cotyledons during germination. Four (4) to six (6) days of germination was the meantime corresponding to maximum hydrolytic activity of this enzyme. The understanding of the role of acid phosphatase activity during germination led to purify this enzyme by successive chromatography separations on DEAE-Sepharose CL-6B, Sephacryl S-100 HR and Phenyl-Sepharose HP to apparent homogeneity from germinated peanut cotyledon five days old. This enzyme designated peanut cotyledon acid phosphatase (AP) had native molecular weight of 24 kDa by gel permeation. SDS-PAGE of the purified acid phosphatase resolved a single protein band that migrated to approximately 21.5 kDa. Thus, this acid phosphatase likely functions as a monomer. The enzyme had optimum pH (5.0) and temperature (55 degrees C), and appeared to be stable in the presence of anionic, cationic and non-ionic detergents. Substrate specificity indicated that the purified acid phosphatase hydrolyzed a broad range of phosphorylated substrates. However, natural substrates such as ADP and ATP were the compounds with highest rate of hydrolysis for the enzyme. Moreover, the purified acid phosphatase exhibited phytase activity. These results showed that this enzyme played a peculiar role during germination, notably in reducing the rate of phytic acid, an antinutritional substance contained in peanut seed.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26908610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of flexibility and long range communication on the function of human topoisomerase I. 灵活性和远程通讯对人体拓扑异构酶I功能的作用。
The Italian journal of biochemistry Pub Date : 2007-06-01
Giovanni Chillemi, Paola Fiorani, Alessandro Bruselles, Silvia Castelli, Alessia Campagna, Ofelia Sarra, Cinzia Tesauro, Monica Fiorentini, Oscar Vassallo, Ilda D'Annessa, Sabrina Santoleri, Alessandro Desideri
{"title":"Role of flexibility and long range communication on the function of human topoisomerase I.","authors":"Giovanni Chillemi,&nbsp;Paola Fiorani,&nbsp;Alessandro Bruselles,&nbsp;Silvia Castelli,&nbsp;Alessia Campagna,&nbsp;Ofelia Sarra,&nbsp;Cinzia Tesauro,&nbsp;Monica Fiorentini,&nbsp;Oscar Vassallo,&nbsp;Ilda D'Annessa,&nbsp;Sabrina Santoleri,&nbsp;Alessandro Desideri","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Eukaryotic topoisomerase I is an essential enzyme that regulates the changes in DNA topology, relaxing the superhelical tension associated with DNA replication, transcription and recombination. Human topoisomerase I is of significant medical interest being the only target of the antitumor drug camptothecin. The enzyme undergoes large conformational changes during its catalytic cycle and the knowedge of the degree of flexibility of the different regions provides an useful guide to the understanding of such movements. Molecular dynamics simulation is a well consolidated method for the investigation of structural and dynamic properties of proteins and nucleic acids and has been successfully applied to study the dynamical properties of the DNA-human topoisomerase complex. This review highlights some structural and dynamic properties of topoisomerase, obtained by MD simulations, that permits to explain the importance of flexibility in the modulation of the functional properties of the enzyme and in the transmission of communication between domains located far away one from each other.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26909566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Variations in the essential oil composition from different parts of Coriandrum sativum L. cultivated in Tunisia. 突尼斯栽培的芫荽不同部位精油成分的变化。
The Italian journal of biochemistry Pub Date : 2007-03-01
Kamel Msaada, Karim Hosni, Mouna Ben Taarit, Thouraya Chahed, Brahim Marzouk
{"title":"Variations in the essential oil composition from different parts of Coriandrum sativum L. cultivated in Tunisia.","authors":"Kamel Msaada,&nbsp;Karim Hosni,&nbsp;Mouna Ben Taarit,&nbsp;Thouraya Chahed,&nbsp;Brahim Marzouk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Essential oils obtained by hydrodistillation from different organs (flowers, leaves, stems and roots) of Tunisian coriander (Coriandrum sativum L.) was analyzed. GC and GC-MS analysis enabled us to identify 64 compounds and revealed great qualitative and quantitative differences between the analyzed parts. In all organs, the main compound was (E)-2-dodecenal, followed by (E)-2-tridecenal, gamma-cadinene, (Z)-myroxide, neryl acetate and eugenol. Multivariate analysis (PCA) revealed a high similarity in the essential oils composition between upper leaves and flowers in one hand and basal leaves, roots and stems on the other hand. Further, it has been reported an organ-dependant distribution of essential oil compounds.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26730855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fenugreek seeds modulate 1,2-dimethylhydrazine-induced hepatic oxidative stress during colon carcinogenesis. 胡芦巴籽在结肠癌发生过程中调节1,2-二甲基肼诱导的肝脏氧化应激。
The Italian journal of biochemistry Pub Date : 2007-03-01
T Devasena, P Venugopal Menon
{"title":"Fenugreek seeds modulate 1,2-dimethylhydrazine-induced hepatic oxidative stress during colon carcinogenesis.","authors":"T Devasena,&nbsp;P Venugopal Menon","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>1,2-dimethylhydrazine (DMH) is a colon carcinogen which undergoes oxidative metabolism in the liver. We have investigated the modulatory effect of fenugreek seeds (a spice) on colon tumor incidence as well as hepatic lipid peroxidation (LPO) and antioxidant status during DMH-induced colon carcinogenesis in male Wistar rats. In DMH treated rats, 100% colon tumor incidence was accompanied by enhanced LPO and a decrease in reduced glutathione (GSH) content as well as a fall in glutathione peroxidase (GPx), glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities. Inclusion of fenugreek seed powder in the diet of DMH treated rats reduced the colon tumor incidence to 16.6%, decreased the LPO and increased the activities of GPx, GST, SOD and CAT in the liver. We report that fenugreek modulates DMH-induced hepatic oxidative stressduring colon cancer</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26730381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification of Euphorbia characias latex peroxidase by calmodulin-affinity chromatography. 钙调素亲和层析纯化大大麻乳胶过氧化物酶。
The Italian journal of biochemistry Pub Date : 2007-03-01
Francesca Pintus, Anna Mura
{"title":"Purification of Euphorbia characias latex peroxidase by calmodulin-affinity chromatography.","authors":"Francesca Pintus,&nbsp;Anna Mura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A new procedure to purify to homogeneity an Euphorbia characias latex peroxidase is performed employing a calmodulin-Sepharose column as affinity chromatography. The advantage of this approach is the isolation of a peroxidase under fast and mild elution conditions with a high recovery in total activity.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26730378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Essential oil composition of Hypericum triquetrifolium Turra. aerial parts. 金丝桃精油成分研究。天线部分。
The Italian journal of biochemistry Pub Date : 2007-03-01
Hosni Karim, Msaada Kamel, Ben Taarit Mouna, Chahed Thouraya, Marzouk Brahim
{"title":"Essential oil composition of Hypericum triquetrifolium Turra. aerial parts.","authors":"Hosni Karim,&nbsp;Msaada Kamel,&nbsp;Ben Taarit Mouna,&nbsp;Chahed Thouraya,&nbsp;Marzouk Brahim","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The essential oil obtained by hydrodistillation from the aerial parts of Tunisian endemic Hypericum triquetrifolium Turra (Clusiaceae) was analyzed using GC and GC-MS. One hundred and nine compounds consisting of 92.2% of total detected constituents were identified. Sesquiterpene hydrocarbons were the main constituents (59.37%), Alpha-humulene, cis-calamenene, delta-cadinene, bicyclogermacrene, eremophilene, betacaryophyllene and (E)-gamma-bisabolene were found as the main ones. Alpha-pinene (10.33%) was detected as the main monoterpene hydrocarbons (12.19%). The oxygenated sesquiterpenes constituted (9.33%); caryophyllene oxide (1.38%) was reported as the main constituent of this fraction. The oxygenated monoterpenes were weakly represented (4.62%) and consisted of constituents in low percentages (<1%).</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26730383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of L-carnitine on RBC membrane composition and function in hyperinsulinemic rats. 左旋肉碱对高胰岛素血症大鼠红细胞膜组成及功能的影响。
The Italian journal of biochemistry Pub Date : 2007-03-01
P Rajasekar, K Balasaraswathi, C V Anuradha
{"title":"Effects of L-carnitine on RBC membrane composition and function in hyperinsulinemic rats.","authors":"P Rajasekar,&nbsp;K Balasaraswathi,&nbsp;C V Anuradha","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The purpose of the study was to investigate the effects of L-carnitine (CA) on the susceptibility of erythrocyte (RBC) to peroxide-induced lipid oxidation, RBC membrane composition, ATPases activity and oxidative stress in fructose-fed hyperinsulinemic rats. The rats were subjected to experimental hyperinsulinemia and hyperglycemia by feeding a high fructose diet (60 g/100 g) for 6 weeks. The rats showed significant alterations in the RBC membrane composition. The protein content was lower than control animals, while cholesterol, phospholipids and free fatty acids were higher in fructose-fed animals. Significant differences in the total carbohydrate and relative proportions of hexose, hexosamine, sialic acid and fucose of membranes were observed. In these rats, membrane-bound ATPases (total ATPase, Na+, K+ ATPase, Mg2+ and Ca2+ ATPases) were significantly lower while thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LHP) in RBC membrane were significantly higher than those of control rats. The red cells were more susceptible to peroxide-induced oxidative stress that correlated with reduced levels of vitamin E found RBC membrane. When fructose-diet fed rats were treated simultaneously with CA (300 mg/kg b.w/day, i.p.), such alterations in membrane composition and enzyme activities did not occur. Effects of fructose loading on lipid peroxidation was also alleviated by CA. These findings suggest that high levels of dietary fructose is detrimental to RBC membrane integrity and that CA may have membrane stabilizing effects in this diet-induced model of type 2-diabetes.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26730856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rat liver xanthine oxidoreductase: effect of adenine on the oxidase and dehydrogenase activities. 大鼠肝黄嘌呤氧化还原酶:腺嘌呤对氧化酶和脱氢酶活性的影响。
The Italian journal of biochemistry Pub Date : 2007-03-01
Kevork Hagopian
{"title":"Rat liver xanthine oxidoreductase: effect of adenine on the oxidase and dehydrogenase activities.","authors":"Kevork Hagopian","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Xanthine oxidase (XO) and total oxidase plus dehydrogenase (XO+XDH) activities from rat liver were measured in the presence or absence of adenine in extracts prepared with or without DTT/PMSF in homogenization buffer. Presence of adenine in extracts, prepared with or without DTT/PMSF, caused a 45-60% decrease in XO and XO+XDH activities. Removal of adenine by dialysis from extracts prepared with or without DTT/PMSF resulted in the recovery of XO and XO+XDH activities to almost their pre-dialysis control levels. Enzyme activity after 24hr storage at -20 degrees C depended on the presence or absence of DTT/PMSF and adenine, with both XO and XO+XDH activities being lower in extracts with the combined presence of DTT/PMSF and adenine. Incubation of extracts at 37 degrees C for 30 minutes resulted in increased XO and XO+XDH activities, however, adenine-treated samples did not differ from their pre-incubation activities. The molecular mass of the enzyme from control and adenine-treated extracts was unchanged (300 kDa). Adenine-treated extracts prepared with or without DTT/PMSF showed higher D/O ratios in all post-dialysis samples when compared with their pre-dialysis ratios. The results suggest that adenine may play a role in preventing the dehydrogenase to oxidase conversion during extract preparation, storage, overnight dialysis and heat treatment.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26730380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plasma levels of adrenomedullin and atrial natriuretic peptide in patients with congestive heart failure of various etiologies. 不同病因的充血性心力衰竭患者血浆肾上腺髓质素和心钠肽水平的变化。
The Italian journal of biochemistry Pub Date : 2007-03-01
M Randa Abdel Kader, B D M Grace, N E S Badawi
{"title":"Plasma levels of adrenomedullin and atrial natriuretic peptide in patients with congestive heart failure of various etiologies.","authors":"M Randa Abdel Kader,&nbsp;B D M Grace,&nbsp;N E S Badawi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to evaluate the plasma levels of the adrenomedullin (ADM) and atrial natriuretic peptide (ANP) in adult and pediatric patients with congestive heart failure (CHF) of various etiologies and to investigate their relations with haemodynamic variables e.g. echocardiographic left ventricular ejection fraction (LVEF) and fractional shortening (FS).</p><p><strong>Subjects and methods: </strong>The study was made in 38 adult and 21 pediatric patients with CHF of various etiologies and compared with 15 adult and 10 pediatric normal healthy controls. Patients with CHF were classified according to the New York Heart Association (NYHA) functional classification into grades II to IV in adult patients and into grade IV in all pediatric patients. ADM and ANP plasma levels were determined prior to the treatment with enzyme immunoassay.</p><p><strong>Results: </strong>A statistically significant difference in the plasma levels of ADM and ANP were found between pediatrics and adult patients and corresponding healthy controls. Their levels were progressively increased with severity of NYHA class in adult patients. We found a significant positive correlation between plasma levels of each of ADM and ANP and pulse rate, systolic and diastolic blood pressure; and a significant negative correlation between their plasma levels and echocardiographic LVEF and FS. A significant positive correlation between plasma levels of ADM and ANP in both pediatrics and adult patients were also found.</p><p><strong>Conclusion: </strong>Plasma levels of ADM and ANP increased in adult and pediatric patients with CHF irrespective of the cause. They were positively correlated with each other and negatively correlated with LVEF and FS. These findings might have important clinical implications in that a noninvasive blood test may be used to identify high-risk subjects for HF for more invasive procedures.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26730379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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