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The Italian journal of biochemistry最新文献

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Simultaneous purification and polymerization method for bovine serum albumin preparation. 牛血清白蛋白制备的同时纯化和聚合方法。
The Italian journal of biochemistry Pub Date : 2007-06-01
Fatemeh Yari, Kamran Mousavi Hosseini
{"title":"Simultaneous purification and polymerization method for bovine serum albumin preparation.","authors":"Fatemeh Yari,&nbsp;Kamran Mousavi Hosseini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bovine serum albumin (BSA) has various applications in blood group serology and different research purposes. In this study purification of BSA has been compared with human serum albumin (HSA) using modified ethanol precipitation method based on the method of Cohn. The purification process was carried out under controlled conditions, particularly of ethanol concentration, pH, ionic strength and temperature. It was revealed that the produced BSA and HSA have purity more than 95%. It is obvious that HSA can be used, as a drug when the amount of its polymers is less than 5% whereas polymer generation is required in order to enhance the potentiating properties of BSA in agglutination of red cells. We propose here a simple and rapid two-step method for simultaneously purification and polymerization of BSA. By this method simply BSA with desired amount of polymers was obtained by 40% ethanol concentration.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26908612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Essential oil composition of two myrtus communis L. varieties grown in North Tunisia. 生长在突尼斯北部的两个桃金娘品种的精油成分。
The Italian journal of biochemistry Pub Date : 2007-06-01
Wissem Aidi Wannes, Baya Mhamdi, Brahim Marzouk
{"title":"Essential oil composition of two myrtus communis L. varieties grown in North Tunisia.","authors":"Wissem Aidi Wannes,&nbsp;Baya Mhamdi,&nbsp;Brahim Marzouk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two Myrtus communis varieties (var. italica and baetica) were studied in order to investigate their essential oil yield and composition. Essential oil yield varied in leaves, fruits and stems. So, in leaves, it was 0.5% for italica and 0.3% for baetica and was higher than in fruits and stems with respectively 0.1% and 0.04% for italica and 0.07% and 0.03% for baetica. The essential oil analysis performed by GC and GC/MS showed a composition characterized by a high percentage of monoterpene hydrocarbons in leaves, largely due to alpha-pinene with 51.3% for italica and 27.7% for baetica; 1,8-cineole, the alone compound of ether class, was predominant in fruits and stems with respectively 31.6% and 34.7% for italica and 19.8% and 25.8% for baetica.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26908616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Estimation of total and direct serum bilirubin using modified micro assay method. 改良微量测定法测定血清总胆红素和直接胆红素。
The Italian journal of biochemistry Pub Date : 2007-06-01
Afsheen Aman, Shah Ali Ul Qader, Saeeda Bano
{"title":"Estimation of total and direct serum bilirubin using modified micro assay method.","authors":"Afsheen Aman,&nbsp;Shah Ali Ul Qader,&nbsp;Saeeda Bano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Estimation of total and direct bilirubin in serum plays an important role in differential diagnosis of hyperbilirubinemia. Several direct spectrophotometric methods are commercially available for total and direct bilirubin estimation in which the amount of the sample (serum) varies from 200 ml to 800 ml. It is difficult to collect such amount of serum from infants, as neonatal jaundice is the most common problem in this age group. To overcome this problem modified micro assay method was developed using dimethylsulfoxide (DMSO). The amount of the serum sample is reduced from 100 ml to 20 ml per test for both total and direct bilirubin. A method comparison study was performed using 100 consecutive serum samples, by modified micro assay method and a reference Jendrassik-Grof method. Total bilirubin in these human serum samples ranged from 0.4-15.0 mg/dl and direct bilirubin ranged from 0.05-12.0 mg/dl. The results conclude that modified micro assay method had significant correlation with r-value of 0.99989 for total serum bilirubin and with r-value of 0.99971 for direct serum bilirubin. Linearity of the method is 20 mg/dl and 15 mg/dl for total and direct bilirubin, respectively. Monoreagent used during the assay is stable for 24 hours at 2-8 degrees C while the kit is stable for one year at 2-8 degrees C. In conclusion this micro assay method is rapid, reliable, simple and accurate for the estimation of total and direct bilirubin with small serum quantities. It is equally reliable for manual; semi automated and automated chemistry analyzers.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26908614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reverse gyrase: an unusual DNA manipulator of hyperthermophilic organisms. 反回转酶:超嗜热生物中一种不寻常的DNA操纵器。
The Italian journal of biochemistry Pub Date : 2007-06-01
Anna D'Amaro, Mosè Rossi, Maria Ciaramella
{"title":"Reverse gyrase: an unusual DNA manipulator of hyperthermophilic organisms.","authors":"Anna D'Amaro,&nbsp;Mosè Rossi,&nbsp;Maria Ciaramella","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Reverse gyrase is the only DNA topoisomerase capable of introducing positive supercoiling into DNA molecules. This unique activity reflects a distinctive arrangement of the protein, which is composed of a topoisomerase IA module fused to a domain containing sequence motives typical of helicases; however, reverse gyrase works neither like a canonical topoisomerase IA nor like a helicase. Extensive genomic analysis has shown that reverse gyrase is present in all organisms living above 70 degrees C and in some of those living at 60- 70 degrees C, but is invariably absent in organisms living at mesophilic temperatures. For its peculiar distribution and biochemical activity, the enzyme has been suggested to play a role in maintenance of genome stability at high temperature. We review here recent phylogenetic, biochemical and structural data on reverse gyrase and discuss the possible role of this enzyme in the biology of hyperthermophilic organisms.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26909565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RecQ helicases and topoisomerases: implications for genome stability in humans. RecQ解旋酶和拓扑异构酶:对人类基因组稳定性的影响。
The Italian journal of biochemistry Pub Date : 2007-06-01
Annapaola Franchitto
{"title":"RecQ helicases and topoisomerases: implications for genome stability in humans.","authors":"Annapaola Franchitto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In recent years growing evidence has suggested that the RecQ helicases play a crucial role in preserving genome stability. The importance of this observation in humans is substantiated by the fact that the absence of a functional RecQ helicase is associated with genetic syndromes characterised by elevated predisposition to a wide variety of cancers. It is well recognized that maintenance of genome integrity relies on the accurate execution of the DNA replication process as well as on efficient DNA repair. A number of studies have described interactions of RecQ helicases with topoisomerases and it has been proposed that this cooperation may be essential for cell viability and the avoidance of cancer development in human cells.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26909567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biochemical evaluation of borage (Borago officinalis) rosette leaves through their essential oil and fatty acid composition. 琉璃苣(Borago officinalis)莲座叶精油和脂肪酸组成的生化评价。
The Italian journal of biochemistry Pub Date : 2007-06-01
Baya Mhamdi, Wissem Aidi Wannes, Brahim Marzouk
{"title":"Biochemical evaluation of borage (Borago officinalis) rosette leaves through their essential oil and fatty acid composition.","authors":"Baya Mhamdi,&nbsp;Wissem Aidi Wannes,&nbsp;Brahim Marzouk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Borago officinalis rosette leaves were sampled in the region of Amdoun (Tunisia) during different stages of their development. Essential oil contents varied from 0.01% to 0.13% respectively in young and adult leaves. Twenty three volatile compounds were identified. Hydrocarbons, mainly represented by nonadecane (29.8%), tetracosane (11.3%) and heptacosane (4.7%), constituted the major class in the young leaves (45.8%), followed by aldehydes (22.4%). The percentages of these two classes decreased to reach respectively 15% and 8.1% in adult leaves in favour of alcohols (57.9%) where cis-3-hexenol (29.6%) and hexanol (14.5%) were the main compounds. Total fatty acids amounts increased from 5.03 mg/g DW in young leaves to 32.23 mg/g DW in adult ones. The predominant fatty acids were alpha-linolenic (C18:3 n-3), stearidonic (C18:4 n-3), gamma-linolenic (C18:3 n-6), palmitic (C16: 0) and linoleic (C18:2 n-6) acids.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26908615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The interplay among chromatin dynamics, cell cycle checkpoints and repair mechanisms modulates the cellular response to DNA damage. 染色质动力学、细胞周期检查点和修复机制之间的相互作用调节了细胞对DNA损伤的反应。
The Italian journal of biochemistry Pub Date : 2007-06-01
Federico Lazzaro, Michele Giannattasio, Marco Muzi-Falconi, Paolo Plevani
{"title":"The interplay among chromatin dynamics, cell cycle checkpoints and repair mechanisms modulates the cellular response to DNA damage.","authors":"Federico Lazzaro,&nbsp;Michele Giannattasio,&nbsp;Marco Muzi-Falconi,&nbsp;Paolo Plevani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cells are continuously under the assault of endogenous and exogenous genotoxic stress that challenges the integrity of DNA. To cope with such a formidable task cells have evolved surveillance mechanisms, known as checkpoints, and a variety of DNA repair systems responding to different types of DNA lesions. These lesions occur in the context of the chromatin structure and, as expected for all DNA transactions, the cellular response to DNA damage is going to be influenced by the chromatin enviroment. In this review, we will discuss recent studies implicating chromatin remodelling factors and histone modifications in the response to DNA double-strand breaks (DSBs) and in checkpoint activation in response to UV lesions.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26909570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of dextransucrase immobilized on calcium alginate beads from Leuconostoc mesenteroides PCSIR-4. 介肠系膜白菌PCSIR-4海藻酸钙微球固定化葡聚糖酶的研究。
The Italian journal of biochemistry Pub Date : 2007-06-01
Shah Ali Ul Qader, Afsheen Aman, Noman Syed, Saeeda Bano, Abid Azhar
{"title":"Characterization of dextransucrase immobilized on calcium alginate beads from Leuconostoc mesenteroides PCSIR-4.","authors":"Shah Ali Ul Qader,&nbsp;Afsheen Aman,&nbsp;Noman Syed,&nbsp;Saeeda Bano,&nbsp;Abid Azhar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immobilization of dextransucrase from Leuconostoc mesenteroides PCSIR-4 on alginate is optimized for application in the production of dextran from sucrose. Dextransucrase was partially purified by ethanol upto 2.5 fold. Properties of dextransucrase were less affected by immobilization on alginate beads from soluble enzyme. Highest activities of both soluble and immobilized dextransucrase found to be at 35 degrees C and optimum pH for activity remain 5.00. Substrate maxima for immobilized enzyme changed from 125 mg/ml to 200 mg/ml. Incubation time for enzyme-substrate reaction for maximum enzyme activity was increased from 15 minutes to 60 minutes in case of immobilized enzyme. Maximum stability of immobilized dextransucrase was achieved at 25 degrees C with respect to time.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26908611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nitrogen effects on proteins, chlorophylls and fatty acids during the growth of Arthrospira platensis. 平节螺旋藻生长过程中氮对蛋白质、叶绿素和脂肪酸的影响。
The Italian journal of biochemistry Pub Date : 2007-06-01
Samah Ayachi, Amor El Abed, Wissal Dhifi, Brahim Marzouk
{"title":"Nitrogen effects on proteins, chlorophylls and fatty acids during the growth of Arthrospira platensis.","authors":"Samah Ayachi,&nbsp;Amor El Abed,&nbsp;Wissal Dhifi,&nbsp;Brahim Marzouk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Spirulina platensis (=Arthrospira platensis) is a tunisian strain which has been isolated for the first time in Oued Essed (Sousse, Sidi Bou Ali). Biomass evolution, proteins, chlorophylls and fatty acids composition of this alga were monitored by varying nitrogen concentrations in the culture medium. Nitrogen stress was provoked by adding sodium nitrate (NaNO3) in the culture medium with concentrations varying from 0 to 5 g/l. Results obtained showed that nitrogen depletion increased total proteins and total chlorophylls. The addition of NaNO3 (5g/l) led to an increase of total fatty acids amounts and modify fatty acids composition. Optimal quantities of palmitic, gamma -linolenic and oleic acids were obtained with NaNO3 free-cultures. Thus, the tunisian strain has valuable biological substances, worthy to determine the optimal conditions for its propagation.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26908613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human DNA topoisomerase IB: structure and functions. 人类DNA拓扑异构酶IB:结构和功能。
The Italian journal of biochemistry Pub Date : 2007-06-01
Erica Cretaio, Luca Pattarello, Yari Fontebasso, Piero Benedetti, Carmen Losasso
{"title":"Human DNA topoisomerase IB: structure and functions.","authors":"Erica Cretaio,&nbsp;Luca Pattarello,&nbsp;Yari Fontebasso,&nbsp;Piero Benedetti,&nbsp;Carmen Losasso","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Human DNA Topoisomerase I is a 765aa monomeric enzyme composed of four domains: the N-terminal domain, highly charged and responsible for several protein-protein interactions, the core domain that embraces the DNA during catalysis, the highly charged linker domain and the C-teminal domain containing the active site. The enzyme promotes the relaxation of supercoiled DNA by nicking and rejoining one of the strands of the DNA. Its activity is critical for many biological processes including DNA replication, transcription, and recombination. The aim of this review is to analyze the enzyme activity in terms of structure-function relationship.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26909564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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