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The Italian journal of biochemistry最新文献

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Methylation profile of P. lividus sea urchin genes during development. 鹅毛海胆发育过程中基因的甲基化特征。
The Italian journal of biochemistry Pub Date : 2003-12-01
Annamaria Piscopo, Giovanna Pulcrano, Francesco Aniello, Margherita Branno, Laura Fucci
{"title":"Methylation profile of P. lividus sea urchin genes during development.","authors":"Annamaria Piscopo,&nbsp;Giovanna Pulcrano,&nbsp;Francesco Aniello,&nbsp;Margherita Branno,&nbsp;Laura Fucci","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Methylation pattern has been studied in two genes of sea urchin Paracentrotus lividus using sodium bisulfite method to understand the possible role of DNA methylation during invertebrate development. Three regions of the gene for the hatching enzyme have been analyzed and all of them resulted unmethylated in embryos at different stages of development. Four CpG rich regions have been studied in the gene for DNA methyltransferase: upstream, upstream-exon1, intron 1 and exon 20. The upstream-exon 1 region is always unmethylated, while intron 1 and exon 20 are heavy methylated. Only the upstream fragment changed its pattern of methylation during development. For none of the studied regions the reported data show a general direct correlation between gene expression and methylation process during development.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24517774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteomic characterization of a wild-type wine strain of Saccharomyces cerevisiae. 酿酒酵母菌野生型酿酒菌株的蛋白质组学研究。
The Italian journal of biochemistry Pub Date : 2003-12-01
Lorenza Trabalzini, Alessandro Paffetti, Elisa Ferro, Andrea Scaloni, Fabio Talamo, Lia Millucci, Paola Martelli, Annalisa Santucci
{"title":"Proteomic characterization of a wild-type wine strain of Saccharomyces cerevisiae.","authors":"Lorenza Trabalzini,&nbsp;Alessandro Paffetti,&nbsp;Elisa Ferro,&nbsp;Andrea Scaloni,&nbsp;Fabio Talamo,&nbsp;Lia Millucci,&nbsp;Paola Martelli,&nbsp;Annalisa Santucci","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Saccharomyces cerevisiae is the optimal eukaryotic model system to study mammalian biological responses. At the same time Saccharomyces cerevisiae is also widely utilized as a biotechnological tool in the food industry. Enological Saccharomyces cerevisiae strains have been so far routinely analyzed for their microbiological aspects. Nevertheless, wine yeasts are gaining an increasing interest in the last years since they strongly affect both the vinification process and the organoleptic properties of the final product wine. The protein repertoire is responsible of such features and, consequently, 2D-PAGE can be an useful tool to evaluate and select optimal wine yeast strains. We present here the first proteomic map of a wild-type wine Saccharomyces cerevisiae strain selected for the guided fermentation of very high quality wines.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24517778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural insights in the folding of small single-domain proteins. 小单结构域蛋白折叠的结构见解。
The Italian journal of biochemistry Pub Date : 2003-12-01
Stefano Gianni, Ugo Mayor, Alan R Fersht
{"title":"Structural insights in the folding of small single-domain proteins.","authors":"Stefano Gianni,&nbsp;Ugo Mayor,&nbsp;Alan R Fersht","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Understanding the mechanism by which a polypeptide chain folds into its unique native structure requires a complete and detailed description of the structural and dynamic properties of all the species populated in the folding process. In the case of small single domain proteins, experimental studies are defining the structures of denatured states, folding intermediates and transition states at nearly atomic resolution. Further, the synergy between theoreticians and experimentalists is now allowing the detailed description of whole (un)folding pathways. Here, we discuss some of the general structural aspects of the denatured states, folding intermediates and transition states that are beginning to emerge from these studies.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24517779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SIBMA 2003: annual meeting of the Marine and Environmental Biochemistry Group. SIBMA 2003:海洋与环境生物化学小组年会。
The Italian journal of biochemistry Pub Date : 2003-12-01
Emilio Carpenè
{"title":"SIBMA 2003: annual meeting of the Marine and Environmental Biochemistry Group.","authors":"Emilio Carpenè","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24517772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SIB 2003. Abstracts of the joint symposia with the British Biochemical Society. Ferrara, Italy. September 15-18, 2003. SIB 2003。与英国生化学会联合专题讨论会摘要。意大利费拉拉。2003年9月15-18日。
The Italian journal of biochemistry Pub Date : 2003-09-01
{"title":"SIB 2003. Abstracts of the joint symposia with the British Biochemical Society. Ferrara, Italy. September 15-18, 2003.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24394554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vibrational characterisation and biological activity of carnosine and its metal complexes. 肌肽及其金属配合物的振动特性和生物活性。
The Italian journal of biochemistry Pub Date : 2003-06-01
Armida Torreggiani, Andrea Trinchero, Maurizio Tamba, Giancarlo Fini
{"title":"Vibrational characterisation and biological activity of carnosine and its metal complexes.","authors":"Armida Torreggiani,&nbsp;Andrea Trinchero,&nbsp;Maurizio Tamba,&nbsp;Giancarlo Fini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The spectroscopic characterisation of free carnosine and its coordination compounds with Cu(II), Zn(II) and Co(II) ions are discussed. Raman and IR studies on metal-carnosine systems have been performed, obtaining a relationship between the vibrational spectra and the structure of the predominant species formed. The biological activity of free carnosine and of its complexes is briefly considered.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24126674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Apoptosis meets proteasome, an invaluable therapeutic target of anticancer drugs. 细胞凋亡与蛋白酶体相遇，成为抗癌药物的宝贵治疗靶点。
The Italian journal of biochemistry Pub Date : 2003-06-01
Michela Giuliano, Antonella D'Anneo, Anna De Blasio, Renza Vento, Giovanni Tesoriere
{"title":"Apoptosis meets proteasome, an invaluable therapeutic target of anticancer drugs.","authors":"Michela Giuliano,&nbsp;Antonella D'Anneo,&nbsp;Anna De Blasio,&nbsp;Renza Vento,&nbsp;Giovanni Tesoriere","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This report reviews the current status of extensive efforts directed towards the interpretation of crosstalk between apoptosis and proteasome to understanding the molecular mechanism of anticancer agents targeting proteasome, with particular focus on MG132 and PS-341. The discovery that all cancer cells have retained the apoptotic death program has offered to the researchers new biochemical targets to design anticancer drugs. Moreover, the demonstration that proteasome inhibition induces apoptosis and sensitizes cancer cells to traditional tumoricidal agents has proposed the proteasome as an attractive target for development of new anticancer drugs. Since then, a number of both naturally occurring and synthetic inhibitors of the proteasome have been identified. The best characterized and most widely used inhibitors of the proteasome are the peptide aldehydes; among these MG132, due to its broad spectrum of action, low cost and rapid reversibility of action, still remains the first choice to study proteasome function in cell and tissue cultures. Recently, a very potent new class of selective and reversible proteasome inhibitors which contains an inhibitory boronate group has been described. PS-341 represent the first of this promising class of agents that could have application in cancer therapy and it is the only that has progressed to clinical trials.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24126678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The origin of dystrophin-glycoprotein complex(DGC)-related muscular dystrophies: the need for protection against an ancestral pathogen? 肌营养不良蛋白-糖蛋白复合物(DGC)相关肌营养不良的起源:需要对祖先病原体进行保护?
The Italian journal of biochemistry Pub Date : 2003-06-01
Andrea Brancaccio
{"title":"The origin of dystrophin-glycoprotein complex(DGC)-related muscular dystrophies: the need for protection against an ancestral pathogen?","authors":"Andrea Brancaccio","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Because of its crucial role during the early stages of morphogenesis, no genetic defects associated to dystroglycan have been reported so far. Dystroglycan is an important member of the dystrophin-glycoprotein complex (DGC) and in several muscular dystrophies, depending on abnormalities of proteins belonging to or associated with the DGC, it is frequently observed a significant reduction of dystroglycan levels at the sarcolemma. Recently, it has been demonstrated that dystroglycan acts as a receptor for pathogens such as M. leprae and arenaviruses. It is well-known that mutated alleles causing diseases can be selected in order to confer an additional genetic advantage. Herein it is discussed the possibility that mutations leading to a certain number of muscular dystrophies might have been originally selected to indirectly gain a specific advantage: the absence or the lower levels of dystroglycan could have greatly reduced the risk of some ancestral lethal infections specifically directed against muscles.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24125531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aminoacid profile and oxidative status in children affected by Down syndrome before and after supplementary nutritional treatment. 补充营养治疗前后唐氏综合征患儿的氨基酸谱和氧化状态。
The Italian journal of biochemistry Pub Date : 2003-06-01
Marcello Ciaccio, Maria Piccione, Mario Giuffrè, Vincenzo Macaione, Lavinia Vocca, Antonino Bono, Giovanni Corsello
{"title":"Aminoacid profile and oxidative status in children affected by Down syndrome before and after supplementary nutritional treatment.","authors":"Marcello Ciaccio,&nbsp;Maria Piccione,&nbsp;Mario Giuffrè,&nbsp;Vincenzo Macaione,&nbsp;Lavinia Vocca,&nbsp;Antonino Bono,&nbsp;Giovanni Corsello","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Down syndrome is the most common autosomal aberration among liveborns, characterised by several clinical features and metabolic disturbances. Aminoacid pathways abnormalities and defective oxidative balance are the most common metabolic problems in Down Syndrome. To evaluate the biochemical responses of children with Down Syndrome to a nutritional regimen supplemented with aminoacids, vitamins and polyunsaturated fatty acids, we submitted 86 subjects divided in two groups (0-6 and 6-12 years) to the dosage of plasma levels of aminoacids, antioxidant enzymes activities and reactive oxygen species, before and after 12 months of such nutritional supplementation and in relation to normal controls. The results obtained showed a tendency towards the values of normal subjects with statistically significant differences. Although other studies must be performed to confirm and define such report, our experience supports the usefulness of a nutritional supplementation with aminoacids, vitamins and polyunsaturated fatty acids, also considering the absence of side effects.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24125532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Imaging transcription complexes with the Atomic Force Microscope. 用原子力显微镜成像转录复合物。
The Italian journal of biochemistry Pub Date : 2003-06-01
Claudio Rivetti, Nicola Vannini, Sara Cellai
{"title":"Imaging transcription complexes with the Atomic Force Microscope.","authors":"Claudio Rivetti,&nbsp;Nicola Vannini,&nbsp;Sara Cellai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Recent developments in sample deposition and image analysis have shown that the Atomic Force Microscope is a valuable tool for the structural investigation of transcription complexes. When deposited under conditions that allow molecular equilibration onto the substrate, transcription complexes behave as worm-like chains and the mean square end-to-end distance can readily be used to determine the protein induced DNA bend angle. Measurements of the DNA contour length by means of accurate image processing procedures have revealed a DNA compaction in transcription complexes which is compatible with wrapping of the DNA against the surface of the RNA Polymerase. The methods presented have to be considered of general practical use for imaging protein-DNA complexes.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24126675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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