发布求助
文献互助 智能选刊 最新文献

The Italian journal of biochemistry最新文献

筛选
英文 中文
Mitochondrial calcium signalling: message of life and death. 线粒体钙信号:生死信息。
The Italian journal of biochemistry Pub Date : 2007-12-01
Erika Zecchini, Roberta Siviero, Carlotta Giorgi, Rosario Rizzuto, Paolo Pinton
{"title":"Mitochondrial calcium signalling: message of life and death.","authors":"Erika Zecchini,&nbsp;Roberta Siviero,&nbsp;Carlotta Giorgi,&nbsp;Rosario Rizzuto,&nbsp;Paolo Pinton","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Upon physiological stimulation, mitochondria undergo a major rise in mitochondrial [Ca2+] ([Ca2+]m) in a wide variety of cell types. Here, particular attention will be focused on the mechanism that allows the low-affinity transporters of mitochondria to rapidly accumulate Ca2+, despite the low amplitude of the cytosolic [Ca2+] ([Ca2+]c) rises, i.e. the close apposition of mitochondria to the Endoplasmic Reticulum (ER), the main pool of agonist-releasable Ca2+. Upon opening of IP3-gated channels, mitochondria are able to sense not the average [Ca2+]c rise, but rather the much higher concentration occurring in the proximity of the open channels. We will then address the functional significance of this process, that spans from the activation of organelle metabolism to the alteration of organelle morphology, and consequent release of pro-apoptotic factors during apoptosis.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27966060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The glutamine/amino acid transporter (ASCT2) reconstituted in liposomes: electrical nature of the glutamine/glutamate antiport. 脂质体中重组的谷氨酰胺/氨基酸转运体(ASCT2):谷氨酰胺/谷氨酸反转运的电学性质。
The Italian journal of biochemistry Pub Date : 2007-12-01
Francesca Oppedisano, Lorena Pochini, Michele Galluccio, Cesare Indiveri
{"title":"The glutamine/amino acid transporter (ASCT2) reconstituted in liposomes: electrical nature of the glutamine/glutamate antiport.","authors":"Francesca Oppedisano,&nbsp;Lorena Pochini,&nbsp;Michele Galluccio,&nbsp;Cesare Indiveri","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The glutamine/amino acid transporter solubilized from rat renal apical plasma membrane (brush-border membrane) with C12E8 and reconstituted into liposomes has been previously identified as the ASCT2 transporter. The reconstituted transporter catalyses an antiport reaction in which extraliposomal glutamine and Na+ are cotransported in exchange with intraliposomal neutral amino acids. Differently from other neutral amino acid transporters, ASCT2 accepts also glutamate as substrate, as demonstrated by the glutamine/glutamate antiport measured in proteoliposomes. The electrical nature of the homologous glutamine/glutamine antiport and of the heterologous glutamine/glutamate antiport has been investigated by imposing a K+ diffusion potential (positive outside) across the proteoliposomal membrane in the presence of valinomycin. The membrane potential did not affect the glutamine/glutamine antiport whereas it stimulated about two fold the glutamine/glutamate antiport rate.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27966066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The smaller isoform of the mitochondrial transcription factor A has a role in the mitochondrial transcription. 线粒体转录因子A的较小同种异构体在线粒体转录中起作用。
The Italian journal of biochemistry Pub Date : 2007-12-01
Stefano Bruno, Caterina De Virgilio, Gemma Gadaleta
{"title":"The smaller isoform of the mitochondrial transcription factor A has a role in the mitochondrial transcription.","authors":"Stefano Bruno,&nbsp;Caterina De Virgilio,&nbsp;Gemma Gadaleta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mitochondrial transcription factor A (Tfam) is a mitochondrial protein encoded in the nucleus. The gene for Tfam spans about 10 kb and consists of seven exons and six introns. In human and rat, exon 5 can splite alternatively resulting in two Tfam isoforms. In order to investigate the role of the delta 5Tfam isoform in human cells, we studied its stability in vitro, then we carried out overexpression experiments in H1299 human cell line in order to clarify the in vivo effect of this shorter isoform of Tfam. The data obtained by Real time-PCR demonstrate that the overexpression of delta 5Tfam causes an increase of mitochondrial transcription, so also this isoform as a role in the mitochondrial process.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27966563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The voltage dependent anion selective channel family in Drosophila melanogaster. 果蝇电压依赖性阴离子选择通道家族。
The Italian journal of biochemistry Pub Date : 2007-12-01
Francesca Guarino, Angela Messina, Andrea Guarnera, Giuseppe Puglia, Francesco Bellia, Simona Reina, Vito De Pinto, Valeria Specchia, Maria Pia Bozzetti
{"title":"The voltage dependent anion selective channel family in Drosophila melanogaster.","authors":"Francesca Guarino,&nbsp;Angela Messina,&nbsp;Andrea Guarnera,&nbsp;Giuseppe Puglia,&nbsp;Francesco Bellia,&nbsp;Simona Reina,&nbsp;Vito De Pinto,&nbsp;Valeria Specchia,&nbsp;Maria Pia Bozzetti","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>VDACs (voltage-dependent anion-selective channels) or mitochondrial PORINS are transmembrane proteins forming pores in the outer membrane. In eukaryotic genomes multiple genes coding for VDAC homologues have been discovered, but the functional meaning of this gene redundancy is unknown. In Drosophila melanogasterthree additional genes homologous to the gene porin (CG6647) have been found. As in other occurences, the presence of a gene revealed by genome analysis raises the questions: are these genes really expressed? What are the molecular features of the putative proteins, if they are expressed? Where and when in the organism are they expressed? Consequently have they any specific activity justifying the presence of more isoforms in the organism? To answer to these questions we have produced antibodies against the recombinant proteins corresponding to the whole (VDAC1 and 2) or to substantial portions (VDAC3 and 4) of the known or predicted proteins. Immunohistological and transcriptional analysis has been performed, showing that VDAC2 and 3 are expressed, while VDAC4 was not detected. Structural predictions of VDAC3 are consistent with the presence of additional alpha-helices at the N-terminus of the protein.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27966067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Confinement of cardiolipin and ubiquinone in reaction-center core complexes purified from the photosynthetic bacterium Rhodobacter sphaeroides. 光合细菌球形红杆菌反应中心核心复合物中心磷脂和泛素的约束。
The Italian journal of biochemistry Pub Date : 2007-12-01
Manuela Dezi, Francesco Francia, Antonia Mallardi, Gerardo Palazzo, Giovanni Venturoli
{"title":"Confinement of cardiolipin and ubiquinone in reaction-center core complexes purified from the photosynthetic bacterium Rhodobacter sphaeroides.","authors":"Manuela Dezi,&nbsp;Francesco Francia,&nbsp;Antonia Mallardi,&nbsp;Gerardo Palazzo,&nbsp;Giovanni Venturoli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The core complex formed by the reaction center and the light harvesting complex 1 (RC-LH1) was purified from the photosynthetic bacterium Rhodobacter sphaeroides. We analyzed the lipid and ubiquinone (UQ) complements copurifying with the RC-LH1 complex and with the peripheral antenna (LH2). In RC-LH1 UQ was almost ten times more concentrated than in the LH2 and in the native membranes from which the complexes were extracted. The fractional lipid composition of the RC-LH1 complex also differed from that of the intact membranes, exhibiting a marked increase in cardiolipin concentration. We propose that the confinement of cardiolipin and ubiquinone observed within the RC-LH1 complex, plays a role in vivo in the stabilization of the light-induced charge separation catalyzed by the RC.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27966063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Is there a pyruvate kinase in pig liver mitochondria? 猪肝线粒体中是否存在丙酮酸激酶?
The Italian journal of biochemistry Pub Date : 2007-12-01
Roberto Pizzuto, Gianluca Paventi, Gabriella Chieppa, Anna Atlante, Salvatore Passarella
{"title":"Is there a pyruvate kinase in pig liver mitochondria?","authors":"Roberto Pizzuto,&nbsp;Gianluca Paventi,&nbsp;Gabriella Chieppa,&nbsp;Anna Atlante,&nbsp;Salvatore Passarella","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In order to ascertain whether mammalian mitochondria possess their own pyruvate kinase, we isolated mitochondria from liver of Large White pig and investigated pyruvate kinase occurrence both via immunological analysis and by assaying photometrically the pyruvate kinase reaction. We show that mitochondria contain pyruvate kinase located in the inner compartments; the pyruvate kinase reaction shows hyperbolic dependence on the substrate concentration, is inhibited by malonate and shows maximum activity at pH between 7-7.6 and Ea equal to 33 kJ/mol.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27966065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional proteomics: protein-protein interactions in vivo. 功能蛋白质组学:体内蛋白质-蛋白质相互作用。
The Italian journal of biochemistry Pub Date : 2007-12-01
Maria Monti, Marianna Cozzolino, Flora Cozzolino, Roberta Tedesco, Piero Pucci
{"title":"Functional proteomics: protein-protein interactions in vivo.","authors":"Maria Monti,&nbsp;Marianna Cozzolino,&nbsp;Flora Cozzolino,&nbsp;Roberta Tedesco,&nbsp;Piero Pucci","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Functional proteomics constitutes an emerging research area in the proteomic field focused to two major targets, the elucidation of biological function of unknown proteins and the definition of cellular mechanisms at the molecular level. Understanding protein functions as well as unravelling molecular mechanisms within the cell is then depending on the identification of the interacting protein partners. The association of an unknown protein with partners belonging to a specific protein complex involved in a particular mechanism would in fact be strongly suggestive of its biological function. Furthermore, a detailed description of the cellular signalling pathways might greatly benefit from the elucidation of protein-protein interactions in the cell. Isolation of functional protein complexes essentially rely on affinity-based procedures. The protein of interest and its specific partners can be fished out from the cellular extract by using a suitable ligand as a bait taking advantage of the specific binding properties of the ligand molecule immobilised on agarose-sepharose supports. Alternative strategies essentially relying on immunoprecipitation techniques have been introduced to allow purification of protein complexes formed in vivo within the cell. The gene coding for the bait tagged with an epitope against which good antibodies exist (FLAG, HA, c-myc, etc.), is transfected into the appropriate cell line and expressed in the cognate host. The cell extracts are immunoprecipitated with anti-tag monoclonal antibodies using suitable experimental conditions to avoid dissociation of the complexes. In both cases, protein components specifically recognised by the bait and retained on the agarose beads can then be eluted and fractionated by SDS-PAGE. The protein bands detected on the gel are in situ enzymatically digested and the resulting peptide mixtures analysed by capillary LC-MS/MS techniques leading to the identification of the protein interactors.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27966562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alpha-bisabolol: unexpected plant-derived weapon in the struggle against tumour survival? -比索洛尔:对抗肿瘤生存的意外植物源武器?
The Italian journal of biochemistry Pub Date : 2007-12-01
Elena Darra, Giorgio Lenaz, Elisabetta Cavalieri, Romana Fato, Sofia Mariotto, Christian Bergamini, Alessandra Carcereri de Prati, Luigi Perbellini, Serena Leoni, Hisanori Suzuki
{"title":"Alpha-bisabolol: unexpected plant-derived weapon in the struggle against tumour survival?","authors":"Elena Darra,&nbsp;Giorgio Lenaz,&nbsp;Elisabetta Cavalieri,&nbsp;Romana Fato,&nbsp;Sofia Mariotto,&nbsp;Christian Bergamini,&nbsp;Alessandra Carcereri de Prati,&nbsp;Luigi Perbellini,&nbsp;Serena Leoni,&nbsp;Hisanori Suzuki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Despite enormous scientific and economic effort tumour still is one of the most terrible pathologies among human population all over the world. Products derived from the plant kingdom have often offered an opportunity to counteract or alleviate this illness. Here, we summarize the short story of the study of an extraordinary effect of one plant compound towards transformed cells derived from highly malignant tumours. Alpha-bisabolol, a sesquiterpene widely present in plants, selectively kills transformed cells by apoptosis without affecting the viability of normal cells. One of its intracellular targets seems to be situated on mitochondria and is possibly identified as the permeability transition pore, as judged from rapid mitochondrial membrane potential dissipation induced by alpha-bisabolol and the failure to kill cells in the presence of cyclosporine A. Preferential adsorption of alpha-bisabolol into lipid rafts, rich in tumour cells, may explain the selective action of this compounds towards tumour cells. Furthermore, Surface Plasmon Resonance analysis indicates that alpha-bisabolol directly interacts with Bid protein, a member of the Bcl2 family deeply involved in apoptosis, suggesting a possibility that Bid, or similar protein(s), may be involved in a putative intracellular transport system of alpha-bisabolol from plasma membrane to mitochondria. Experiments with animals indicate that alpha-bisabolol is not toxic and is accumulated, through blood flow, in every tissues examined. Further animal studies to test its effect are currently under way.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27964913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural characterization of the transmembrane segments of the mitochondrial oxoglutarate carrier (OGC) by NMR spectroscopy. 线粒体氧戊二酸载体(OGC)跨膜片段的核磁共振结构表征。
The Italian journal of biochemistry Pub Date : 2007-12-01
Maria Antonietta Castiglione Morelli, Angela Ostuni, Francesca Armentano, Ferdinando Palmieri, Faustino Bisaccia
{"title":"Structural characterization of the transmembrane segments of the mitochondrial oxoglutarate carrier (OGC) by NMR spectroscopy.","authors":"Maria Antonietta Castiglione Morelli,&nbsp;Angela Ostuni,&nbsp;Francesca Armentano,&nbsp;Ferdinando Palmieri,&nbsp;Faustino Bisaccia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The oxoglutarate carrier (OGC) is a member of the mitochondrial carrier protein superfamily, which includes the ADP/ATP carrier and other functionally characterized members, and exchanges cytosolic malate for 2-oxoglutarate from the mitochondrial matrix. By means of CD and NMR spectroscopy, we previously characterized four synthetic peptides containing transmembrane segments (TMSs) I, II, V and VI of the OGC, respectively, in TFE/water mixtures and SDS micelles. Here, we present data on the remaining transmembrane segments of OGC obtained by performing CD and NMR studies on peptides corresponding to TMS-III and TMS-IV. In TFE/water, alpha-helical structures were found for these peptides in the L121-R146 and T187-S201 regions, respectively. We also evaluated the compatibility between the helical structures of our peptides and a homology model of the OGC based on the available X-ray structure of the ATP/ADP carrier.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27966557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of reactive oxygen species as signal molecules in the pre-commitment phase of adult stem cells. 活性氧作为信号分子在成体干细胞分化前阶段的作用。
The Italian journal of biochemistry Pub Date : 2007-12-01
Claudia Piccoli, Annamaria D'Aprile, Rosella Scrima, Maria Ripoli, Domenico Boffoli, Antonio Tabilio, Nazzareno Capitanio
{"title":"Role of reactive oxygen species as signal molecules in the pre-commitment phase of adult stem cells.","authors":"Claudia Piccoli,&nbsp;Annamaria D'Aprile,&nbsp;Rosella Scrima,&nbsp;Maria Ripoli,&nbsp;Domenico Boffoli,&nbsp;Antonio Tabilio,&nbsp;Nazzareno Capitanio","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This mini-review summarizes evidence, provided by our group, relevant to the understanding of how redox signalling may control the fate of adult hematopoietic stem/progenitor cells (HSPCs). In particular it is shown that bone marrow-derived human HSPC are endowed with a composite panel of constitutively active NADPH-oxidases (NOXs) comprising the cell membrane-localized catalytic subunits of the NOX1, NOX2 and NOX4 isoforms. It is proposed that the coordinated activity of the NOX isoforms in HSPCs function as environmental oxygen sensor and generate low level of ROS, which likely serve as second messengers. The pro-oxidant setting, entering into play when HSPCs leave the hypoxic bone marrow niche, would enable them to be more responsive to proliferative/differentiative stimuli. Moreover it is suggested that enhanced ROS elicit mitochondrial \"differentiation\" in a pre-commitment phase needed to match the bioenergetic request in the oncoming proliferation/differentiation process.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27966559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信