Synthetic and Systems Biotechnology最新文献

{"title":"A smart gene circuit for precise regulation of tumor cell behavior","authors":"Hao Fu ,&nbsp;Zhiwei Zhou ,&nbsp;Lan Fang ,&nbsp;Qing Wang ,&nbsp;Xin Tang ,&nbsp;Wei Su ,&nbsp;Xuancai Chen ,&nbsp;Yachun Tang ,&nbsp;Qun Zhou","doi":"10.1016/j.synbio.2025.09.016","DOIUrl":"10.1016/j.synbio.2025.09.016","url":null,"abstract":"<div><div>Personalized gene circuit is a robust mode of cellular regulation that can manipulate intracellular gene expression to achieve desired functional regulation. However, the construction of logic circuits that automatically sense the characteristics of a particular environment within a cell is often difficult and lacking in sensitivity. Here, we synthesize from scratch specific promoters capable of sensing in cells, and use the combination of different types of promoters to construct smart gene circuits that can regulate gene expression in specific cell types sensitively. In detail, the tumour-specific promoter and the prostate tissue-specific promoter were constructed to be combined together into generating an artificial AND-gate gene circuit using the CRISPR-Cas9 system which could identify prostate cancer selectively. We then utilized this artificial gene circuit to drive targeted genes expression, such as P21, E-cadherin and Bax, to inhibit multifunctional prostate cancer cells but not other cells. Moreover, we applied gene circuits to redirect endogenous genes within cells and significantly and specifically suppressed the tumor growth of prostate cancer in vivo. Overall, these results highlight the clinical potential of these gene circuits as specific tools for prostate cancer detection and treatment, which is a new method for specifically reprogramming prostate cancer cells <em>in vivo</em> and may serve as a promising treatment strategy.</div></div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"11 ","pages":"Pages 237-246"},"PeriodicalIF":4.4,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced microbial production of pyridoxine (Vitamin B6) in Bacillus subtilis via pathway and process optimization","authors":"Ai-Tong Jiang ,&nbsp;Guang-Qing Du ,&nbsp;Xu-Yang Huang ,&nbsp;Zheng-Zi Ji ,&nbsp;Si-Riguleng Qian ,&nbsp;Lin-Xia Liu ,&nbsp;Da-Wei Zhang","doi":"10.1016/j.synbio.2025.09.014","DOIUrl":"10.1016/j.synbio.2025.09.014","url":null,"abstract":"<div><div>Vitamin B₆ refers to a family of water-soluble B vitamin, which is essential for various physiological functions, including amino acid metabolism, neurotransmitter synthesis, and hemoglobin synthesis. The biosynthesis of pyridoxine (PN), a commercial form of vitamin B₆, through microbial fermentation has garnered widespread attention owing to its environmentally friendly and safe production methods, as well as its mild reaction conditions. However, the low yield of natural strains limits their application. This study focused on constructing a high-yielding strain of PN through pathway engineering and process optimization. Firstly, five key deoxyxylulose-5-phosphate-dependent pathway genes (<em>epd</em>, <em>pdxB</em>, <em>serC</em>, <em>pdxA</em>, and <em>pdxJ</em>) were overexpressed in <em>Bacillus subtilis</em> ARTP, which improved the PN titer by 3.2-fold to 2.9 mg/L. Subsequently, <em>pdxST</em> genes involved in the DXP-independent pathway were screened from various strains. Ribosome binding site (RBS) sequences were optimized to regulate their expression, which further increased the PN titer to 24.6 mg/L. Finally, systematic medium optimization was identified as a critical strategy for enhancing PN biosynthesis, leading to a remarkable 1.8-fold improvement in PN production. Under optimized fermentation conditions, the engineered strain achieved a PN titer of 174.6 mg/L in fed-batch fermentation, which represents the highest level reported to date in <em>B</em>. <em>subtilis</em>. Overall, this study presents an effective strategy combining pathway engineering and medium optimization for significantly improving PN production, offering valuable insights for the industrial development of PN biosynthesis.</div></div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"11 ","pages":"Pages 216-225"},"PeriodicalIF":4.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis","authors":"Hao Zha ,&nbsp;Yanjie Li ,&nbsp;Zhongmei Hu,&nbsp;Jiacheng Li,&nbsp;Yujie Xie,&nbsp;Mingtao Zhao,&nbsp;Lili Ren,&nbsp;Biao Zhang","doi":"10.1016/j.synbio.2025.09.015","DOIUrl":"10.1016/j.synbio.2025.09.015","url":null,"abstract":"<div><div><em>Kluyveromyces marxianus</em> is a promising thermotolerant yeast for industrial biotechnology, but lacks efficient genome engineering tools. A CRISPR/Cas12a genome editing toolbox for <em>K</em>. <em>marxianus</em> was developed for the first time in this study. A plasmid-free transient system achieved single-gene knockout efficiencies of about 50 %–100 % in <em>Δku70</em> strain. Even with homology arms as short as 35 bp, the knockout efficiency remained 66.67 %. Chromosomal integration of Cas12a enabled single-to-triple fragment knock-ins efficiency of 82.93–85.70 % and 94.50 % for large fragment (&gt;5 kb) integrations. Applying this system, the roles of succinate dehydrogenase (<em>SDH</em>) genes <em>SDH1-SDH5</em> were elucidated. Combinatorial <em>SDH</em> genes knockouts redirected carbon flux toward succinic acid (SA), but increased glycerol/acetate byproducts. Subsequent <em>GPD1</em>/<em>ACH1/ADH2A</em> co-knockout in a <em>Δsdh1,3,5,4A,2</em> strain with <em>NDE1</em> overexpression (YZH43) yielded a chassis producing 32.38 g/L SA from glucose at 37 °C, which is the highest reported titer in <em>K. marxianus</em>, while reducing ethanol, acetate, and glycerol by 60.79 %, 89.24 %, and 67.5 %, respectively. At 46 °C, YZH43 produced 20.51 g/L SA through simultaneous saccharification and fermentation (SSF) using cellulose as substrate. This work provides a high-efficiency CRISPR/Cas12a platform for <em>K. marxianus</em>, enabling rapid metabolic engineering for value-added chemical production, and demonstrates its utility in developing thermotolerant SA-overproducing strains.</div></div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"11 ","pages":"Pages 193-204"},"PeriodicalIF":4.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Overproduction of endusamycin in Streptomyces endus subsp. aureus” [Synth Syst Biotechnol 10 (2) (2025) 523–531]","authors":"Yingying Chang ,&nbsp;Zhen Liu ,&nbsp;Zixin Deng ,&nbsp;Tiangang Liu","doi":"10.1016/j.synbio.2025.09.001","DOIUrl":"10.1016/j.synbio.2025.09.001","url":null,"abstract":"","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"10 4","pages":"Page 1438"},"PeriodicalIF":4.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient synthesis of l-DOPA in Escherichia coli via cofactor and enzyme engineering","authors":"Lihao Deng ,&nbsp;Jurong Ping ,&nbsp;Zhuoyuan Liu ,&nbsp;Kai Linghu ,&nbsp;Heng Zhang ,&nbsp;Xiaoyu Shan ,&nbsp;Weizhu Zeng ,&nbsp;Jianghua Li ,&nbsp;Jingwen Zhou","doi":"10.1016/j.synbio.2025.09.011","DOIUrl":"10.1016/j.synbio.2025.09.011","url":null,"abstract":"<div><div>The global incidence of Parkinson's disease continues to rise. Levodopa (<span>l</span>-DOPA) is the core therapeutic drug, and efficient and sustainable production methods are needed. However, the complex metabolic pathways and the low catalytic efficiency of enzymes limit biosynthesis of <span>l</span>-DOPA in microorganisms. To address this issue, this study significantly enhanced the production efficiency of <span>l</span>-DOPA through a multi-dimensional, integrated metabolic and enzyme engineering approach. Firstly, the <em>de novo</em> synthesis pathway for <span>l</span>-DOPA was established through optimization of the promoter, ribosome-binding site (RBS), plasmid copy number, and tighly accurately regulating the expression level of key enzymes. Secondly, combined with metabonomic analysis, carbon metabolic flow was diverted, increasing the <span>l</span>-DOPA titer by 36.7 %. Glucose dehydrogenase (<em>BmgdH</em>) and gluconate kinase (<em>gntK</em>) were introduced to construct a cofactor regeneration system, which synergistically enhanced the supply of NADH and FADH₂, increasing the <span>l</span>-DOPA conversion rate by 18 %. Next, the substrate tunnel of 4-hydroxyphenylacetic acid-3-monooxygenase subunit B (HpaB) was subjected to rational design, and mutant T292A significantly expanded the substrate channel, improved catalytic efficiency, and decreased <span>l</span>-tyrosine by 87 %. Finally, through the process optimization in a 5 L bioreactor (involving phased pH control and induction timing adjustment) achieved an <span>l</span>-DOPA titer of 60.73 g/L, the highest reported to date for <em>de novo</em> microbial synthesis. This research offers a novel approach for industrial biosynthesis of <span>l</span>-DOPA, and broadens engineering concepts for efficient synthesis of aromatic compounds.</div></div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"11 ","pages":"Pages 226-236"},"PeriodicalIF":4.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced PrrAB system activation and restricted farnesyl pyrophosphate diversion underlie high coenzyme Q10 accumulation in Rhodobacter sphaeroides HY01","authors":"Xinwei He ,&nbsp;Huangwei Wang ,&nbsp;Mindong Liang ,&nbsp;Weishan Wang ,&nbsp;Biqin Chen ,&nbsp;Dan Li ,&nbsp;Lixin Zhang ,&nbsp;Gao-Yi Tan","doi":"10.1016/j.synbio.2025.09.012","DOIUrl":"10.1016/j.synbio.2025.09.012","url":null,"abstract":"<div><div>The industrial <em>Rhodobacter sphaeroides</em> HY01 accumulates an exceptionally high level of coenzyme Q₁₀ (Q₁₀), but the underlying mechanisms remain incompletely understood. Given the central role of Q₁₀ in respiratory electron transport, previous observation of reduced expression of <em>cbb</em>_<em>3</em>-type cytochrome <em>c</em> oxidase genes in HY01 suggested a potential mechanistic link. In this study, we found that <em>cbb</em>_<em>3</em> oxidase activity in HY01 was only 21.8–32.8 % of that in the wild-type 2.4.1, and restoring this activity led to a 64.4 % decrease in Q₁₀ accumulation, demonstrating a strong inverse correlation. This correlation was found to be mediated by the activation of the PrrAB two-component regulatory system, which is negatively regulated by <em>cbb</em>_<em>3</em> oxidase. However, disruption of <em>cbb</em>_<em>3</em> oxidase in 2.4.1 alone was insufficient to reproduce the high Q₁₀ accumulation phenotype, indicating that additional factors may be required. Previous research also revealed restricted synthesis of geranylgeranyl diphosphate (GGPP) in HY01, which likely reduces the diversion of the Q₁₀ precursor farnesyl diphosphate (FPP). Reconstituting this metabolic constraint in wild-type strain, combined with fine-tuning of PrrAB system activation, resulted in up to a 218.0 % increase in Q₁₀ accumulation, achieving a level nearly identical to HY01. Combining mechanistic investigation and inverse metabolic engineering, this study demonstrates that the high Q₁₀ accumulation in HY01 results from the synergistic effects of enhanced PrrAB activation and restricted FPP diversion, providing new insights into the key factors underlying high-level Q₁₀ accumulation in <em>R</em>. <em>sphaeroides.</em></div></div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"11 ","pages":"Pages 172-180"},"PeriodicalIF":4.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroDFBEST: A dCas12b-derived dual-function base editor with programmable editing characteristics for microbial genetic engineering","authors":"Wen-Liang Hao ,&nbsp;De-Zhi Geng ,&nbsp;Yu-Feng Liu ,&nbsp;Lai-Chuang Han ,&nbsp;Zhe-Min Zhou ,&nbsp;Wen-Jing Cui","doi":"10.1016/j.synbio.2025.09.013","DOIUrl":"10.1016/j.synbio.2025.09.013","url":null,"abstract":"<div><div>Base editors (BEs) enable precise genome editing, but their use in microbes remains limited by restricted mutagenesis capabilities and narrow editing windows. Here, we reported MicroDFBEST, a novel dual-function base editor (DFBE) for microbes, by fusing the high-activity deaminases evoCDA1 and TadA9 with nuclease-deficient Cas12b from <em>Bacillus hisashii</em> (dBhCas12b). This engineered system enables simultaneous C-to-T and A-to-G editing within a 26–33 nt window, the broadest range reported for microbial DFBEs. The editing characteristics of MicroDFBEST can be easily adjusted by changing fusion protein expression and editing generations to create diverse mutant libraries. We show that the MicroDFBEST system enables both flexible gene expression modulation via random promoter (P_{<em>ylbP</em>}) diversification and targeted protein evolution through mutational hotspot scanning in native genomic contexts. This study offers a versatile platform enabling in situ gene regulation (e.g., biosynthetic gene clusters activation) and protein evolution (e.g., chassis optimization), with broad synthetic biology utility.</div></div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"11 ","pages":"Pages 161-171"},"PeriodicalIF":4.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-cell biosensors with regulatory circuits based on a synthetic dual-input promoter enabling the highly sensitive detection of ultra-trace Cd2+","authors":"Yuke Qin ,&nbsp;Shuting Hu ,&nbsp;Yibin Yang ,&nbsp;Xiaoqiang Jia","doi":"10.1016/j.synbio.2025.09.008","DOIUrl":"10.1016/j.synbio.2025.09.008","url":null,"abstract":"<div><div>The research on whole-cell biosensors tailored for trace and ultra-trace detection remains limited and the biosensors based on natural bacterial heavy metal resistance mechanisms have common issues of low sensitivity. In this study, we designed a promoter P_{<em>T7-cadO</em>} using the Cadmium ions (Cd²⁺)-binding protein binding site <em>cadO</em> with the T7 promoter at first and constructed a single-input whole-cell biosensor, which was named CP100, whose detection limit for Cd²⁺ met the WHO requirement, yet its response and sensitivity were quite low. We further introduced the <em>lacI</em> and <em>lac operator</em> (<em>lacO</em>) as the signal amplifier to construct a dual-input promoter P_{<em>T7-cadO-lacO-cadO</em>} and developed a biosensor named LC100, whose response and sensitivity were significantly improved, but background leakage became a new problem. Then we redesigned the gene circuit based on the regulatory circuit LCPM-2, which has the structure of “CadR-P_{<em>J23100</em>}-P_{<em>T7-cadO-lacO-cadO</em>}-mRFP1-LacI”, with the LacI protein as the autoregulatory negative feedback model and finally obtained the biosensor named LC100-2, which achieved the detection of ultra-trace Cd²⁺ (0.00001–0.02 nM), with the sensitivity of 3748.22 times that of CP100. Moreover, LC100-2 demonstrated excellent specificity to Cd²⁺ among four other divalent metal ions and good anti-interference capability in the mixed divalent metal ions system. The results of the real water sample tests demonstrated that precise quantitative detection of Cd²⁺ with a final concentration of 0.001–0.02 nM could be achieved by adding only a small volume of the sample (1 μL). This finding showed promising application potential in the field of trace detection. Additionally, the regulatory circuit LCPM-2 based on the unique dual-input promoter enhanced responses and reduced background leakage simultaneously, thereby providing an innovative strategy for the design and simplification of biosensors’ circuits.</div></div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"11 ","pages":"Pages 181-192"},"PeriodicalIF":4.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145159022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient trans-aconitic acid production using systematically metabolic engineered Escherichia coli","authors":"Leilei Guo ,&nbsp;Yi Cheng ,&nbsp;Xiaoxu Tan ,&nbsp;Hongxu Zhang ,&nbsp;Hairong Yu ,&nbsp;Wenjia Tian ,&nbsp;Mingyuan Liu ,&nbsp;Weikang Sun ,&nbsp;Kaiyu Gao ,&nbsp;Tianyi Jiang ,&nbsp;Qianjin Kang ,&nbsp;Wensi Meng ,&nbsp;Yidong Liu ,&nbsp;Zhaoqi Kang ,&nbsp;Chuanjuan Lü ,&nbsp;Chao Gao ,&nbsp;Ping Xu ,&nbsp;Cuiqing Ma","doi":"10.1016/j.synbio.2025.09.009","DOIUrl":"10.1016/j.synbio.2025.09.009","url":null,"abstract":"<div><div><em>trans</em>-Aconitic acid (TAA) is a versatile platform biochemical exhibiting extensive applications. In this work, <em>Escherichia coli</em> W3110 (DE3) was metabolic modified for the de novo biosynthesis of TAA. Firstly, a pyruvate accumulation chassis strain <em>E. coli</em> W3110-P6 was constructed through deletion of six byproducts generation genes. Secondly, the aconitate isomerase was screened from four candidates and co-overexpressed with TAA transporter to construct heterologous TAA biosynthetic pathway in <em>E. coli</em> W3110-P6. Thirdly, the genes <em>pyc</em>^P458S encoding a feedback-insensitive pyruvate carboxylase and <em>gltA</em> encoding an allosterically unaffected citrate synthase were overexpressed, and <em>aceA</em> encoding the isocitrate lyase was deleted, to increase precursor supply and TAA generation of the recombinant <em>E. coli</em>. Finally, the fermentation condition of the obtained strain <em>E. coli</em> W3110-TAA08 was optimized. TAA at a concentration of 37.32 g/L was generated within 40 h, with a yield of 0.76 g/g glucose and a productivity of 0.93 g/L/h.</div></div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"11 ","pages":"Pages 110-116"},"PeriodicalIF":4.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid combinatorial assembly method for gene cluster characterisation illuminates glidobactin biosynthesis","authors":"Mark J. Calcott ,&nbsp;Jonas Kröger ,&nbsp;David F. Ackerley","doi":"10.1016/j.synbio.2025.09.010","DOIUrl":"10.1016/j.synbio.2025.09.010","url":null,"abstract":"<div><div>Heterologous expression of natural product gene clusters in tractable hosts offers great promise for achieving sustainable production of nature-inspired drugs. However, it is common for the roles of some genes in a cluster to be unclear, and this can make it difficult to identify the minimal gene set required to produce the desired molecule. Typically, the function of unknown genes is inferred by time-consuming reductionist techniques, e.g. single and multiple gene knockouts in the native producer, followed by phenotype analysis. Here, we instead present a rapid combinatorial method to assemble individual genes involved in glidobactin biosynthesis into a collection of partial or complete clusters in a heterologous host. Following up with mass spectrometry allowed identification of the minimum genes required for compound production. We applied this synthetic biology approach to characterise the glidobactin gene cluster, for which previous gene knockout studies had yielded conflicting results. In the process, we showed that an added intrinsic advantage of combinatorial assembly is the generation of multiple strains that produce potentially desirable analogues in addition to glidobactin.</div></div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"11 ","pages":"Pages 85-90"},"PeriodicalIF":4.4,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}