Synthetic and Systems Biotechnology最新文献_第10页

A highly efficient method for genomic deletion across diverse lengths in thermophilic Parageobacillus thermoglucosidasius 嗜热副乳杆菌不同长度基因组删除的高效方法

IF 4.8 2区生物学

Synthetic and Systems Biotechnology Pub Date : 2024-05-17 DOI: 10.1016/j.synbio.2024.05.009

Zhiheng Yang , Bixiao Li , Ruihong Bu , Zhengduo Wang , Zhenguo Xin , Zilong Li , Lixin Zhang , Weishan Wang

{"title":"A highly efficient method for genomic deletion across diverse lengths in thermophilic Parageobacillus thermoglucosidasius","authors":"Zhiheng Yang , Bixiao Li , Ruihong Bu , Zhengduo Wang , Zhenguo Xin , Zilong Li , Lixin Zhang , Weishan Wang","doi":"10.1016/j.synbio.2024.05.009","DOIUrl":"10.1016/j.synbio.2024.05.009","url":null,"abstract":"<div>Parageobacillus thermoglucosidasius is emerging as a highly promising thermophilic organism for metabolic engineering. The utilization of CRISPR-Cas technologies has facilitated programmable genetic manipulation in P. thermoglucosidasius. However, the absence of thermostable NHEJ enzymes limited the capability of the endogenous type I CRISPR-Cas system to generate a variety of extensive genomic deletions. Here, two thermophilic NHEJ enzymes were identified and combined with the endogenous type I CRISPR-Cas system to develop a genetic manipulation tool that can achieve long-range genomic deletion across various lengths. By optimizing this tool—through adjusting the expression level of NHEJ enzymes and leveraging our discovery of a negative correlation between GC content of the guide RNA (gRNA) and deletion efficacy—we streamlined a comprehensive gRNA selection manual for whole-genome editing, achieving a 100 % success rate in randomly selecting gRNAs. Notably, using just one gRNA, we achieved genomic deletions spanning diverse length, exceeding 200 kilobases. This tool will facilitate the genomic manipulation of P. thermoglucosidasius for both fundamental research and applied engineering studies, further unlocking its potential as a thermophilic cell factory.</div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"9 4","pages":"Pages 658-666"},"PeriodicalIF":4.8,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405805X2400084X/pdfft?md5=c0ba3cfd0579149a88026a1bb7d806af&pid=1-s2.0-S2405805X2400084X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141051563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae 漆酶是酵母中合成基因回路的多任务蛋白质

IF 4.8 2区生物学

Synthetic and Systems Biotechnology Pub Date : 2024-05-14 DOI: 10.1016/j.synbio.2024.05.007

Lifang Yu , Michael Dare Asemoloye , Mario Andrea Marchisio

{"title":"Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae","authors":"Lifang Yu , Michael Dare Asemoloye , Mario Andrea Marchisio","doi":"10.1016/j.synbio.2024.05.007","DOIUrl":"10.1016/j.synbio.2024.05.007","url":null,"abstract":"<div>Laccase is a multicopper oxidase enzyme that oxidizes a variety of substrates, including polyphenols and polycyclic aromatic hydrocarbons (PAHs). It catalyzes the four-electron reduction of molecular oxygen that results in the production of water as a by-product. Thus, laccase can play an important role in environmental care. Previously, we have successfully expressed Trametes trogii laccase (TtLcc1) in the yeast Saccharomyces cerevisiae. In this work, we have expressed in yeast another laccase, LacA from Trametes sp. AH28-2, and tested its function on PAHs. Yeast cells engineered to produce the two laccases performed efficient PAH degradation. Both TtLcc1 and LacA led to the construction of spatiotemporal fluorescence-pulse generators when combined with a benzoate/salicylate yeast biosensor in a two-population system. Moreover, laccases returned a visual output signal in yeast synthetic circuits—upon reacting with ABTS (2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)). Thus, in S. cerevisiae, laccases are a powerful alternative to fluorescent reporter proteins.</div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"9 4","pages":"Pages 638-646"},"PeriodicalIF":4.8,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405805X24000826/pdfft?md5=94b4a3b771a037d03930700fdbebb1a0&pid=1-s2.0-S2405805X24000826-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141042589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical synthesis of taxanes from mevalonate 从甲羟戊酸中生化合成紫杉类化合物

IF 4.8 2区生物学

Synthetic and Systems Biotechnology Pub Date : 2024-05-13 DOI: 10.1016/j.synbio.2024.05.002

Jing Li , Xiaonan Liu , Xiaoxi Zhu , Jiayu Liu , Lei Zhang , Nida Ahmed , Jian Qi , Bihuan Chen , Daliang Tang , Jinsheng Yu , Zhijin Fan , Huifeng Jiang

{"title":"Biochemical synthesis of taxanes from mevalonate","authors":"Jing Li , Xiaonan Liu , Xiaoxi Zhu , Jiayu Liu , Lei Zhang , Nida Ahmed , Jian Qi , Bihuan Chen , Daliang Tang , Jinsheng Yu , Zhijin Fan , Huifeng Jiang","doi":"10.1016/j.synbio.2024.05.002","DOIUrl":"10.1016/j.synbio.2024.05.002","url":null,"abstract":"<div>Taxanes are kinds of diterpenoids with important bioactivities, such as paclitaxel (taxol®) is an excellent natural broad-spectrum anticancer drug. Attempts to biosynthesize taxanes have made with limited success, mainly due to the bottleneck of the low efficiency catalytic elements. In this study, we developed an artificial synthetic system to produce taxanes from mevalonate (MVA) by coupling biological and chemical methods, which comprises in vitro multi-enzyme catalytic module, chemical catalytic module and yeast cell catalytic module. Through optimizing in vitro multienzyme catalytic system, the yield of taxadiene was increased to 946.7 mg/L from MVA within 8 h and the productivity was 14.2-fold higher than microbial fermentation. By incorporating palladium catalysis, the conversion rate of Taxa-4(20),11(12)-dien-5α-yl acetate (T5α-AC) reached 48 %, effectively addressing the product promiscuity and the low yield rate of T5αOH. Finally, we optimized the expression of T10βOH in yeast resulting in the biosynthesis of Taxa-4(20),11(12)-dien-5α-acetoxy-10β-ol(T5α-AC-10β-ol) at a production of 15.8 mg/L, which displayed more than 2000-fold higher than that produced by co-culture fermentation strategy. These technologies offered a promising new approach for efficient synthesis of taxanes.</div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"9 4","pages":"Pages 694-700"},"PeriodicalIF":4.8,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405805X2400070X/pdfft?md5=9bc12b054aaa058a77e0a0cdc2b83996&pid=1-s2.0-S2405805X2400070X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141037752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New N-acylated aminoalkanoic acids from tea roots derived biocontrol agent Clonostachys rosea 15020 从茶叶根部提取的新型 N-酰化氨基烷酸生物控制剂 Clonostachys rosea 15020

IF 4.8 2区生物学

Synthetic and Systems Biotechnology Pub Date : 2024-05-11 DOI: 10.1016/j.synbio.2024.05.006

Jiaming Yu , Yue Zhang , Li Zhang , Jie Shi , Kun Wang , Weize Yuan , Zexu Lin , Shangqian Ning , Bohao Wang , Xinye Wang , Yuyang Qiu , Tom Hsiang , Lixin Zhang , Xueting Liu , Guoliang Zhu

{"title":"New N-acylated aminoalkanoic acids from tea roots derived biocontrol agent Clonostachys rosea 15020","authors":"Jiaming Yu , Yue Zhang , Li Zhang , Jie Shi , Kun Wang , Weize Yuan , Zexu Lin , Shangqian Ning , Bohao Wang , Xinye Wang , Yuyang Qiu , Tom Hsiang , Lixin Zhang , Xueting Liu , Guoliang Zhu","doi":"10.1016/j.synbio.2024.05.006","DOIUrl":"10.1016/j.synbio.2024.05.006","url":null,"abstract":"<div>Four new N-acylated aminoalkanoic acids, namely clonoroseins E−H (1−4), together with three previously identified analogs, clonoroseins A, B, and D (5−7), were identified from the endophytic fungus Clonostachys rosea strain 15020 (CR15020), using Feature-based Molecular Networking (FBMN). The elucidation of their chemical structures, including their absolute configurations, was achieved through spectroscopic analysis combined with quantum chemical calculations. Bioinformatics analyses suggested that an iterative type I HR-PKS (CrsE) generates the polyketide side chain of these clonoroseins. Furthermore, a downstream adenylate-forming enzyme of the PKS (CrsD) was suspected to function as an amide synthetase. CrsD potentially facilitates the transformation of the polyketide moiety into an acyl-AMP intermediate, followed by nucleophilic substitution with either β-alanine or γ-aminobutyric acid to produce amide derivatives. These findings significantly expand our understanding of PKS-related products originating from C. rosea and also underscore the powerful application of FBMN analytical methods in characterization of new compounds.</div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"9 4","pages":"Pages 684-693"},"PeriodicalIF":4.8,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405805X24000814/pdfft?md5=01cef55bed96e72c42a2b3604900de88&pid=1-s2.0-S2405805X24000814-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141027199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The LysR family transcriptional regulator ORF-L16 regulates spinosad biosynthesis in Saccharopolyspora spinosa LysR家族转录调控因子ORF-L16调控刺五加的生物合成

IF 4.8 2区生物学

Synthetic and Systems Biotechnology Pub Date : 2024-05-10 DOI: 10.1016/j.synbio.2024.05.001

Xin Mu , Ru Lei , Shuqing Yan , Zixin Deng , Ran Liu , Tiangang Liu

{"title":"The LysR family transcriptional regulator ORF-L16 regulates spinosad biosynthesis in Saccharopolyspora spinosa","authors":"Xin Mu , Ru Lei , Shuqing Yan , Zixin Deng , Ran Liu , Tiangang Liu","doi":"10.1016/j.synbio.2024.05.001","DOIUrl":"https://doi.org/10.1016/j.synbio.2024.05.001","url":null,"abstract":"<div>Spinosad, a potent broad-spectrum bioinsecticide produced by Saccharopolyspora spinosa, has significant market potential. Despite its effectiveness, the regulatory mechanisms of spinosad biosynthesis remain unclear. Our investigation identified the crucial role of the LysR family transcriptional regulator ORF-L16, located upstream of spinosad biosynthetic genes, in spinosad biosynthesis. Through reverse transcription PCR (RT-PCR) and 5′-rapid amplification of cDNA ends (5′-Race), we unveiled that the spinosad biosynthetic gene cluster (BGC) contains six transcription units and seven promoters. Electrophoretic mobility shift assays (EMSAs) demonstrated that ORF-L16 bound to seven promoters within the spinosad BGC, indicating its involvement in regulating spinosad biosynthesis. Notably, deletion of ORF-L16 led to a drastic reduction in spinosad production from 1818.73 mg/L to 1.69 mg/L, accompanied by decreased transcription levels of spinosad biosynthetic genes, confirming its positive regulatory function. Additionally, isothermal titration calorimetry (ITC) and EMSA confirmed that spinosyn A, the main product of the spinosad BGC, served as an effector of ORF-L16. Specifically, it decreased the binding affinity between ORF-L16 and spinosad BGC promoters, thus exerting negative feedback regulation on spinosad biosynthesis. This research enhances our comprehension of spinosad biosynthesis regulation and lays the groundwork for future investigations on transcriptional regulators in S. spinosa.</div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"9 4","pages":"Pages 609-617"},"PeriodicalIF":4.8,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405805X24000693/pdfft?md5=7b0b5533316d925759dda110575da5c6&pid=1-s2.0-S2405805X24000693-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140914436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica 油脂酵母亚罗酵母基因表达和操作的进展与前景

IF 4.8 2区生物学

Synthetic and Systems Biotechnology Pub Date : 2024-05-10 DOI: 10.1016/j.synbio.2024.05.003

Mengchen Hu, Jianyue Ge, Yaru Jiang, Xiaoman Sun, Dongshen Guo, Yang Gu

{"title":"Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica","authors":"Mengchen Hu, Jianyue Ge, Yaru Jiang, Xiaoman Sun, Dongshen Guo, Yang Gu","doi":"10.1016/j.synbio.2024.05.003","DOIUrl":"https://doi.org/10.1016/j.synbio.2024.05.003","url":null,"abstract":"<div>The utilization of industrial biomanufacturing has emerged as a viable and sustainable alternative to fossil-based resources for producing functional chemicals. Moreover, advancements in synthetic biology have created new opportunities for the development of innovative cell factories. Notably, Yarrowia lipolytica, an oleaginous yeast that is generally regarded as safe, possesses several advantageous characteristics, including the ability to utilize inexpensive renewable carbon sources, well-established genetic backgrounds, and mature genetic manipulation methods. Consequently, there is increasing interest in manipulating the metabolism of this yeast to enhance its potential as a biomanufacturing platform. Here, we reviewed the latest developments in genetic expression strategies and manipulation tools related to Y. lipolytica, particularly focusing on gene expression, chromosomal operation, CRISPR-based tool, and dynamic biosensors. The purpose of this review is to serve as a valuable reference for those interested in the development of a Y. lipolytica microbial factory.</div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"9 4","pages":"Pages 618-626"},"PeriodicalIF":4.8,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405805X24000711/pdfft?md5=c495d2ca89fba481587179d504cd2b75&pid=1-s2.0-S2405805X24000711-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140918634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous activation and metabolites identification of the pks7 gene cluster from Saccharopolyspora erythraea 红球藻 pks7 基因簇的异源激活和代谢物鉴定

IF 4.4 2区生物学

Synthetic and Systems Biotechnology Pub Date : 2024-05-09 DOI: 10.1016/j.synbio.2024.05.004

Hao Tang , Xingchi Yang , Wenzong Wang , Xingjun Cui , Wenping Wei , Jing Wu , Peng Sun , Bang-Ce Ye

引用次数: 0

Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters 合理构建基因组最小化链霉菌宿主以表达次生代谢物基因簇

IF 4.8 2区生物学

Synthetic and Systems Biotechnology Pub Date : 2024-05-05 DOI: 10.1016/j.synbio.2024.04.017

Hui Li , Sheng Gao , Sanyuan Shi , Xiaomin Zhao , Haoyu Ye , Yunzi Luo

{"title":"Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters","authors":"Hui Li , Sheng Gao , Sanyuan Shi , Xiaomin Zhao , Haoyu Ye , Yunzi Luo","doi":"10.1016/j.synbio.2024.04.017","DOIUrl":"https://doi.org/10.1016/j.synbio.2024.04.017","url":null,"abstract":"<div>Streptomyces offer a wealth of naturally occurring compounds with diverse structures, many of which possess significant pharmaceutical values. However, new product exploration and increased yield of specific compounds in Streptomyces have been technically challenging due to their slow growth rate, complex culture conditions and intricate genetic backgrounds. In this study, we screened dozens of Streptomyces strains inhabiting in a plant rhizosphere for fast-growing candidates, and further employed CRISPR/Cas-based engineering techniques for stepwise refinement of a particular strain, Streptomyces sp. A-14 that harbors a 7.47 Mb genome. After strategic removal of nonessential genomic regions and most gene clusters, we reduced its genome size to 6.13 Mb, while preserving its growth rate to the greatest extent. We further demonstrated that cleaner metabolic background of this engineered strain was well suited for the expression and characterization of heterologous gene clusters, including the biosynthetic pathways of actinorhodin and polycyclic tetramate macrolactams. Moreover, this streamlined genome is anticipated to facilitate directing the metabolic flux towards the production of desired compounds and increasing their yields.</div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"9 3","pages":"Pages 600-608"},"PeriodicalIF":4.8,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405805X24000681/pdfft?md5=96b78023849d6969617af7fe24b33ed0&pid=1-s2.0-S2405805X24000681-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Multiplex Genome Loci Editing of Halomonas bluephagenesis Using an Engineered CRISPR-guided Base Editor 使用工程化 CRISPR 引导的碱基编辑器同时对蓝光单胞菌进行多重基因组位点编辑

IF 4.8 2区生物学

Synthetic and Systems Biotechnology Pub Date : 2024-04-25 DOI: 10.1016/j.synbio.2024.04.016

Yulin Zhang , Yang Zheng , Qiwen Hu , Zhen Hu , Jiyuan Sun , Ping Cheng , Xiancai Rao , Xiao-Ran Jiang

{"title":"Simultaneous Multiplex Genome Loci Editing of Halomonas bluephagenesis Using an Engineered CRISPR-guided Base Editor","authors":"Yulin Zhang , Yang Zheng , Qiwen Hu , Zhen Hu , Jiyuan Sun , Ping Cheng , Xiancai Rao , Xiao-Ran Jiang","doi":"10.1016/j.synbio.2024.04.016","DOIUrl":"10.1016/j.synbio.2024.04.016","url":null,"abstract":"<div>Halomonas bluephagenesis TD serves as an exceptional chassis for next generation industrial biotechnology to produce various products. However, the simultaneous editing of multiple loci in H. bluephagenesis TD remains a significant challenge. Herein, we report the development of a multiple loci genome editing system, named CRISPR-deaminase-assisted base editor (CRISPR-BE) in H. bluephagenesis TD. This system comprises two components: a cytidine (CRISPR-cBE) and an adenosine (CRISPR-aBE) deaminase-based base editor. CRISPR-cBE can introduce a cytidine to thymidine mutation with an efficiency of up to 100% within a 7-nt editing window in H. bluephagenesis TD. Similarly, CRISPR-aBE demonstrates an efficiency of up to 100% in converting adenosine to guanosine mutation within a 7-nt editing window. CRISPR-cBE has been further validated and successfully employed for simultaneous multiplexed editing in H. bluephagenesis TD. Our findings reveal that CRISPR-cBE efficiently inactivated all six copies of the IS1086 gene simultaneously by introducing stop codon. This system achieved an editing efficiency of 100% and 41.67% in inactivating two genes and three genes, respectively. By substituting the Pcas promoter with the inducible promoter PMmp1, we optimized CRISPR-cBE system and ultimately achieved 100% editing efficiency in inactivating three genes. In conclusion, our research offers a robust and efficient method for concurrently modifying multiple loci in H. bluephagenesis TD, opening up vast possibilities for industrial applications in the future.</div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"9 3","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405805X2400067X/pdfft?md5=b15c2dd720390fb769b69b577cbe14b6&pid=1-s2.0-S2405805X2400067X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140785797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a non-model yeast Rhodotorula mucilaginosa for terpenoids synthesis 改造非模式酵母 Rhodotorula mucilaginosa 以合成萜类化合物

IF 4.8 2区生物学

Synthetic and Systems Biotechnology Pub Date : 2024-04-23 DOI: 10.1016/j.synbio.2024.04.015

Qiongqiong Chen , Liting Lyu , Haizhao Xue , Aabid Manzoor Shah , Zongbao Kent Zhao

{"title":"Engineering a non-model yeast Rhodotorula mucilaginosa for terpenoids synthesis","authors":"Qiongqiong Chen , Liting Lyu , Haizhao Xue , Aabid Manzoor Shah , Zongbao Kent Zhao","doi":"10.1016/j.synbio.2024.04.015","DOIUrl":"https://doi.org/10.1016/j.synbio.2024.04.015","url":null,"abstract":"<div>Terpenoids have tremendous biological activities and are widely employed in food, healthcare and pharmaceutical industries. Using synthetic biology to product terpenoids from microbial cell factories presents a promising alternative route compared to conventional methods such as chemical synthesis or phytoextraction. The red yeast Rhodotorula mucilaginosa has been widely studied due to its natural production capacity of carotenoid and lipids, indicating a strong endogenous isoprene pathway with readily available metabolic intermediates. This study constructed several engineered strains of R. mucilaginosa with the aim of producing different terpenoids. Monoterpene α-terpineol was produced by expressing the α-terpineol synthase from Vitis vinifera. The titer of α-terpineol was further enhanced to 0.39 mg/L by overexpressing the endogenous rate-limiting gene of the MVA pathway. Overexpression of α-farnesene synthase from Malus domestica, in combination with MVA pathway rate-limiting gene resulted in significant increase in α-farnesene production, reaching a titer of 822 mg/L. The carotenoid degradation product β-ionone was produced at a titer of 0.87 mg/L by expressing the β-ionone synthase from Petunia hybrida. This study demonstrates the potential of R. mucilaginosa as a platform host for the direct biosynthesis of various terpenoids and provides insights for further development of such platforms.</div>","PeriodicalId":22148,"journal":{"name":"Synthetic and Systems Biotechnology","volume":"9 3","pages":"Pages 569-576"},"PeriodicalIF":4.8,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405805X24000668/pdfft?md5=59f13b657d4fdba39f6b86f484b19421&pid=1-s2.0-S2405805X24000668-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140644175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0