Synapse最新文献

{"title":"Inhibition of miR-378a-3p Protects Anesthesia-Induced Hippocampal Neurodegeneration.","authors":"Junxiao Chang, Quan Lin, Yanyan Jiang, Dalong Wang, Lei Che, Meiqing Du, Shuai Wang, Yu Zhang","doi":"10.1002/syn.70027","DOIUrl":"https://doi.org/10.1002/syn.70027","url":null,"abstract":"<p><p>Ketamine is widely used in pediatric anesthesia, but it may induce cortical neuronal damage, leading to apoptosis, neurofibrillary degeneration, and even cell death. miRNAs can regulate the neurotoxicity induced by ketamine in hippocampal neurons. This article explores the role of miR-378a-3p in ketamine-induced impairments, providing a reference for preventing hippocampal neurodegenerative lesions induced by anesthesia. The expression of miR-378a-3p and AdipoR1 was detected in hippocampal tissues and HT22 cells. To confirm the targeting interaction between miR-378a-3p and AdipoR1, dual-luciferase reporter assays were performed. Cellular viability and apoptosis were also assessed. Ketamine upregulates the expression of miR-378a-3p and downregulates the expression of AdipoR1 in hippocampal tissues and cells, impairing cognitive function in rats. Following ketamine induction, the levels of TNF-α, IL-1β, IL-6, caspase-3, ROS, and MDA are increased, while the level of SOD is decreased in rat hippocampal tissues and cells. Knocking down miR-378a-3p inhibits oxidative stress injury and the release of inflammatory factors by upregulating the expression of AdipoR1. These findings indicate that inhibition of miR-378a-3p protects hippocampal neurons from ketamine-induced damage by upregulating AdipoR1.This suggests that inhibiting miR-378a-3p protects hippocampal neuronal cells from Ketamine-induced damage by upregulating AdipoR1. The study indicates that miR-378a-3p/AdipoR1 axis is a crucial pathway regulating ketamine-induced cognitive impairments and hippocampal neurodegenerative lesions.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 5","pages":"e70027"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-138-5p Alleviates Abnormal Pain and Neuroinflammation in Postherpetic Neuralgia by Inhibiting ROCK2.","authors":"Shuang Chen, Qiu Jin, Xia Geng, Xiaona Guo, Tingting Wang, Jingjing Xu, Linkai Jiang, Qing Su","doi":"10.1002/syn.70031","DOIUrl":"https://doi.org/10.1002/syn.70031","url":null,"abstract":"<p><p>Postherpetic neuralgia (PHN) is a form of neuropathic pain that has significant detrimental effects. This study seeks to explore the potential association between miR-138-5p and PHN. A PHN model was established by infecting rats with the varicella-zoster virus. Following this, the expression level of miR-138-5p in spinal cord was quantified using RT-qPCR. To further investigate its role, miR-138-5p levels were modulated through the intrathecal administration of a lentivirus. The abnormal pain sensitivity in the rats was assessed utilizing the paw withdrawal threshold (PWT). Additionally, the levels of glial fibrillary acidic protein (GFAP) and pro-inflammatory cytokines (IL-1β and TNF-α) in spinal cord were measured by RT-qPCR or enzyme-linked immunosorbent assay (ELISA). Dual-luciferase reporter assay was used to verify the binding relationship between miR-138-5p and ROCK2. miR-138-5p is downregulated in the spinal cord tissue of PHN rats. Notably, the overexpression of miR-138-5p significantly enhances the PWT in PHN rats. Furthermore, the elevation of miR-138-5p markedly mitigates the abnormal increase of GFAP and pro-inflammatory factors. Mechanistically, ROCK2 has been identified as a downstream target of miR-138-5p. During the onset of PHN, ROCK2 is persistently upregulated, whereas the overexpression of miR-138-5p effectively inhibits this increase. Interestingly, the concurrent overexpression of miR-138-5p and ROCK2 can counteract the enhancement in PWT engendered solely by the upregulation of miR-138-5p, alongside the reduction in levels of GFAP, IL-1β, and IL-6. miR-138-5p plays a crucial role in modulating the development of PHN. In the context of PHN, miR-138-5p inhibits spinal cord inflammation and hyperalgesia by suppressing ROCK2.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 5","pages":"e70031"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetylation of Histones in the Nucleus Accumbens Increases the Expression of Dopamine Transporters to Regulate Executive Function in Sapap3 Knockout Mice.","authors":"Hanrui Shi, Jianqun Fang, Yanrong Wang, Yundong Chen, Yuan Wang, Deshang Dai","doi":"10.1002/syn.70029","DOIUrl":"https://doi.org/10.1002/syn.70029","url":null,"abstract":"<p><p>Obsessive-compulsive disorder (OCD) is a chronic and debilitating psychiatric condition characterized by persistent, intrusive thoughts (obsessions) and repetitive ritualistic behaviors (compulsions). Accumulating evidence suggests that individuals with OCD demonstrate marked cognitive impairments, especially in executive function domains, including cognitive flexibility and working memory. Although existing therapeutic approaches (e.g., serotonin reuptake inhibitors and cognitive-behavioral therapy) can partially mitigate behavioral symptoms, their effectiveness in improving cognitive impairments remains limited. Although dopaminergic dysregulation has been implicated in OCD, the involvement of dopamine transporter (DAT) polymorphisms in cognitive flexibility and working memory impairments remains unclear, limiting the development of targeted therapeutic interventions. Although current investigations predominantly focus on dopamine (DA) D1/D2 receptors and serotonin transporters, the contribution of the DAT to OCD pathophysiology remains insufficiently explored. In the present study, we utilized Sapap3 knockout (KO) mice as a preclinical OCD model to examine dopaminergic dysregulation. Using ELISA, we conducted systematic comparisons of both dopaminergic levels and DAT expression between Sapap3 KO and wild-type (WT) mice. Additionally, we implemented targeted epigenetic modulation via histone acetylation in the nucleus accumbens (NAc) of Sapap3 KO mice. Our data demonstrated a significant decrease in DAT expression in the NAc of Sapap3 KO mice compared to WT (p = 0.0019). Strikingly, the administration of the histone deacetylase inhibitor 4-phenylbutyric acid (PBA) normalized DAT expression in KO mice to levels statistically equivalent to WT mice (p = 0.1107), achieving complete functional recovery of DAT deficiency. This restoration of DAT expression (p = 0.1107 PBA vs. WT) was accompanied by a significant reduction in the abnormally elevated baseline DA levels in Sapap3 KO mice (pretreatment: 192.46 ± 6.69 pg/mg; posttreatment: 173.67 ± 4.10 pg/mg, p < 0.01), which consequently improved executive function impairments. Our findings demonstrate that DAT polymorphisms represent a previously unrecognized pathogenic factor in cognitive impairment associated with OCD. Investigations of histone acetylation regulatory mechanisms revealed that changes in histone acetylation levels directly regulate DAT expression. We provide mechanistic evidence showing that epigenetic regulation of DAT expression can effectively reverse these neurological deficits, particularly through modulation of histone acetylation status to significantly improve DAT functional abnormalities, thereby identifying a novel and promising therapeutic target for OCD treatment.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 5","pages":"e70029"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine-Induced Behavioral Effects on Tail Suspension Test, BDNF, and CREB Levels in the Prefrontal Cortex, Hippocampus, and Amygdala: Modulation by Central Serotonergic Transmission.","authors":"Venu Anand Das Vaishnav, Richa Patel, Vaibhav Maturkar, Chhatrapal Patel, Nishant Sudhir Jain","doi":"10.1002/syn.70028","DOIUrl":"https://doi.org/10.1002/syn.70028","url":null,"abstract":"<p><p>Berberine has demonstrated an antidepressant-like effect in rodents. Moreover, it increases central 5-hydroxytryptamine (5-HT)/brain-derived neurotrophic factor (BDNF) or cyclic AMP response element binding protein (CREB) levels, but the exact role of these targets in its effect is still ill-explained. Therefore, the present study explored the role of 5-HT in berberine-induced antidepressant-like activity and BDNF or CREB expression in the specific brain regions. Administration of berberine (2, 5, and 20 mg/kg, i.p.) significantly reduced the duration of immobility of mice in the tail suspension test (TST) model without affecting locomotor activity, confirming its antidepressant-like potential. Preinjection with the 5-HT precursor, 5-hydroxytryptophan (5-HTP; 50 and 100 µg/mouse, intracerebroventricular [i.c.v.]), and the selective serotonin reuptake inhibitor (SSRI), fluoxetine (5 and 10 mg/kg, i.p.), enhanced the berberine-induced antidepressant-like effect, whereas it was reversed in mice pretreated with 5-HT_1A receptor agonist, 8-hydroxy-2(-dipropylamino)tetralinhydrobromide (8-OH-DPAT) (0.01 and 0.1 µg/mouse, i.c.v.), or the 5-HT_2A/2C receptor antagonist, mianserin (1, 1.5 µg/mouse, i.c.v.). Furthermore, berberine-induced antidepressant-like effects were significantly reduced in mice with depleted central 5-HT, achieved by preinjection of the serotonin depletor, p-chlorophenylalanine (p-CPA; 300 mg/kg, i.p. × 3 days). Additionally, berberine (20 mg/kg, i.p.) treatment significantly elevated BDNF expression in the whole brain, prefrontal cortex (PFC), and hippocampus and increased CREB expression in the whole brain and hippocampus only. Notably, berberine-induced elevated BDNF expression in the whole brain and hippocampus was further enhanced in mice pretreated with 5-HTP, fluoxetine, or 8-OH-DPAT, whereas mianserin or p-CPA reversed it. However, 5-HTP enhanced and fluoxetine or 8-OH-DPAT did not alter, whereas mianserin or p-CPA reversed the berberine-induced heightened BDNF expression in the PFC. On the other hand, berberine significantly increased CREB expression in the whole brain, which was further potentiated in mice pretreated with 5-HTP, fluoxetine, 8-OH-DPAT, or mianserin, whereas it was reversed by p-CPA. However, berberine-induced heightened CREB expression in the hippocampus was enhanced only in mice pretreated with 5-HTP and attenuated by preinjection of mianserin or p-CPA. These findings suggest that serotonergic transmission modulates the antidepressant-like activity and the BDNF/CREB alterations induced by berberine, likely through a mechanism mediated by 5-HT_1A and 5-HT_2A/2C receptors. This study underscores the role of serotonergic transmission in the effects induced by berberine.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 5","pages":"e70028"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[¹²⁵I]IPPI for Tau Imaging: Binding Studies in Postmortem Human Alzheimer's Disease Hippocampus-Subiculum and Evaluation of Drug Effects.","authors":"Fariha Karim, Brooke A Delaney, Rommani Mondal, Christopher Liang, Geidy E Serrano, Thomas G Beach, Jogeshwar Mukherjee","doi":"10.1002/syn.70024","DOIUrl":"10.1002/syn.70024","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is characterized by the accumulation of tau tangles that aggregate into neurofibrillary tangles (NFT). This study aims to assess binding of [¹²⁵I]IPPI, a recently reported imaging probe for pathological, aggregated tau in AD human hippocampus (HP) postmortem brain slices, and measure effects of drugs known to bind to tau, monoamine oxidase A (MAO-A), and dual specificity tyrosine-phosphorylation regulated kinase 1A (DYRK1A). Quantitative [¹²⁵I]IPPI binding was compared between AD (n = 29; 13 male and 16 female) and cognitively normal (CN) (n = 32; 16 male and 16 female) subjects. Significantly, there was more [¹²⁵I]IPPI binding in AD gray matter (GM), which positively correlated with the percent of anti-tau immunostaining. GM/white matter (WM) ratios in AD were higher compared to CN subjects. Female AD (avg. GM/WM = 2.42) exhibited greater [¹²⁵I]IPPI binding compared to males (avg. GM/WM = 1.91). Binding of [¹²⁵I]IPPI increased with Braak neurofibrillary stages of the subjects, and the effects of aging were mixed, with females showing a downward trend. A positive correlation between [¹²⁵I]IPPI binding to tau and [¹⁸F]flotaza binding to amyloid beta (Aβ) suggests potential pathophysiological associations between the two AD biomarkers. MK-6240 (aggregated tau-selective) and harmine (MAO-A, DYRK1A, and tau nonselective) inhibited [¹²⁵I]IPPI binding by 88% and 69%, respectively. No effect on [¹²⁵I]IPPI binding was observed by selective DYRK1A (KuFal194) and MAO-A (clorgyline) inhibitors. Affinity of harmine for tau binding sites was quantified by inhibitor concentration (IC₅₀) = 135 ± 29 nM. This study demonstrates promise of radiolabeled IPPI as a viable and selective tau radiotracer to assist in the diagnostic imaging of AD in humans.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 4","pages":"e70024"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12230280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulation of miR-22-3p Alleviates Hyperalgesia and Neuroinflammation Caused by Migraine.","authors":"Lijun Yang, Feng Li, Linlin Guo, Shengnan Qi, Pengcheng Liu","doi":"10.1002/syn.70017","DOIUrl":"https://doi.org/10.1002/syn.70017","url":null,"abstract":"<p><strong>Objective: </strong>Migraines profoundly impact patients' quality of life. This study seeks to investigate the relationship between dysregulated miR-22-3p and the neuroinflammation and central sensitization associated with migraine.</p><p><strong>Methods: </strong>Initially, the level of miR-22-3p in migraine patients were analyzed using RT-qPCR. Subsequently, a migraine model was established by administering nitroglycerin (NTG) to mice. To modulate the levels of miR-22-3p within this model, agomir was utilized. Following this intervention, mechanical and thermal pain sensitivity were evaluated by Von Frey filament and radiant heat. The levels of c-Fos, CGRP, TNF-α, IL-1β, and IL-6 in trigeminal nucleus caudalis (TNC) were detected by RT-qPCR and ELISA. Furthermore, dual luciferase reporting assays were conducted to ascertain whether miR-22-3p could target KLF6. Moreover, the influence of KLF6 on inflammatory cytokines and central sensitization were further studied.</p><p><strong>Results: </strong>miR-22-3p was significantly reduced in migraine patients and NTG mice. In animals, overexpression of miR-22-3p significantly alleviated hyperalgesia and neuroinflammation induced by NTG. Following the overexpression of miR-22-3p, we observed an increase in thermal withdrawal latency, paw mechanical threshold, and periorbital mechanical threshold. Conversely, levels of c-Fos, CGRP, TNF-α, IL-1β, and IL-6 exhibited a significant reduction. We found that miR-22-3p can inhibit KLF6 expression. Additionally, further findings indicated that the suppression of KLF6 resulted in decreased pain sensitivity along with diminished expression of c-Fos, CGRP, TNF-α, IL-1β, and IL-6.</p><p><strong>Conclusion: </strong>In the context of migraine, miR-22-3p may play a pivotal role in mitigating neuroinflammation and alleviating central sensitization through the inhibition of KLF6.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 3","pages":"e70017"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased Expression of Synaptic Vesicle Glycoprotein 2A (SV2A) in the Brain of Chronic Diabetic Rats.","authors":"Burcu Azak Pazarlar, Cansu Bilister Egilmez, Eser Öz Oyar, Jens D Mikkelsen","doi":"10.1002/syn.70018","DOIUrl":"https://doi.org/10.1002/syn.70018","url":null,"abstract":"<p><strong>Aim/hypothesis: </strong>Diabetes mellitus has been reported to be a risk factor for cognitive dysfunction, depression, stroke, and seizures. Diabetic pathology is believed to interfere with synaptic plasticity. Synaptic vesicle glycoprotein 2A (SV2A) is a presynaptic vesicular protein and a popular synaptic density imaging marker. We investigated the effect of chronic hyperglycemia on the expression of SV2A in the cerebral cortex and hippocampus of rats and compared it to other presynaptic markers, such as GAP43, Synaptotagmin-1, and SNAP25.</p><p><strong>Methods: </strong>A single dose of streptozotocin (STZ, 45 mg/kg, i.p.) was administered to adult male rats, resulting in sustained hyperglycemia and reduced plasma insulin levels. Controls were injected with saline, and another STZ group was treated with insulin. Fasting blood glucose (FBG) and fasting plasma insulin (FPI) levels were monitored throughout the observation period, and the level of SV2A was determined by radioligand, [³H]UCB-J, binding capacity using in-vitro autoradiography and by ELISA. Similarly, the tissue concentration of other synaptic proteins GAP43, SNAP25, and SYN1 was measured using ELISA. Quantitative RT-qPCR was performed to measure Sv2a, Sv2b, and Sv2c transcripts. Finally, hippocampal and cortical glutamate levels were measured in all tissues.</p><p><strong>Results: </strong>[³H]UCB-J binding, SV2A (pg/mg protein) and Sv2a mRNA levels were significantly higher in hyperglycemic rats. The SV2A concentration detected by ELISA and [³H]UCB-J binding showed, as expected, a positive correlation with each other. The same positive and significant correlation was seen between SV2A, FBG, and glutamate l levels across animals (p ≤ 0.001). Notably, there was no difference and no linearity between FBG and other presynaptic markers such as GAP43, Synaptotagmin-1, and SNAP25.</p><p><strong>Conclusions: </strong>Unlike other synaptic markers (e.g., SNAP25, SYN-1), SV2A levels rise independently of synaptic density, correlating with elevated glutamate and metabolic activity. These findings raise doubt about SV2A's role as a pure synaptic density marker.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 3","pages":"e70018"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroanatomical Correlates of Memory Strategies in Rats.","authors":"Aysu Balcı, Buse Pınar Cankurtaran, Aybüke Akyel, Kutluk Bilge Arıkan, Bengi Ünal, Çağrı Temuçin Ünal","doi":"10.1002/syn.70015","DOIUrl":"10.1002/syn.70015","url":null,"abstract":"<p><p>Navigation incorporates a continuum of strategies, where the allocentric strategy relies on relationships between environmental landmarks resulting in a cognitive map, and the egocentric strategy revolves around the body position and stimulus response chains with the body as a reference. Although multiple brain regions contribute to navigation, the hippocampus dominates allocentric navigation, whereas the striatum is key for egocentric navigation. Neuromodulators, such as dopamine and acetylcholine, regulate both the hippocampus and striatum to influence behavior, yet their influence on navigational strategy has not been determined. Interindividual differences in strategy preference are known to exist. Building on these pre-existing interindividual differences, this study explored the neuroanatomical underpinnings on navigational strategy variations in rats through a dual-solution T-maze and immunocytochemistry. Surprisingly, interindividual variations eluded explanation through the density of cholinergic neurons supplying acetylcholine to the hippocampus and striatum. Similarly, the soma morphologies of these neurons exhibited no discernible differences. Dopaminergic cell densities in the ventral tegmental area (VTA), projecting to the hippocampus, and substantia nigra pars compacta (SNpc), projecting to the striatum, failed to account for individual variations as well. Nevertheless, allocentric rats displayed higher VTA/SNpc dopamine neuron fusiformity indexes, potentially contributing to computational distinctions underlying interindividual variations in navigation strategies. This study delves into potential explanations and charts promising avenues for future research. A graphical abstract summarizing the main findings of this study is provided.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 3","pages":"e70015"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synaptosomal-Associated Protein 25 kDA (SNAP-25) Levels in Cerebrospinal Fluid: Implications for Alzheimer's Disease Diagnosis and Monitoring.","authors":"Sofia Hjorth Wolner, Helena Sophia Gleerup, Christian Sandøe Musaeus, Peter Høgh, Nicholas J Ashton, Ann Brinkmalm, Johanna Nilsson, Lana Grötschel, Henrik Zetterberg, Kaj Blennow, Steen Gregers Hasselbalch, Anne Byriel Walls, Anja Hviid Simonsen","doi":"10.1002/syn.70010","DOIUrl":"10.1002/syn.70010","url":null,"abstract":"<p><p>Synaptic degeneration has been linked to cognitive decline. The presynaptic protein, synaptosomal-associated protein 25 kDA (SNAP-25), is crucial for synaptic transmission and has been suggested as a biomarker in Alzheimer's disease (AD). In the current study, we investigated the ability of SNAP-25 to differentiate between heterogenous dementia etiologies and whether SNAP-25 could be a staging marker in AD. SNAP-25 in the cerebrospinal fluid (CSF) from a retrospective (n = 187) and a prospective (n = 134) cohort was investigated with immunoprecipitation mass spectrometry (IP-MS) and single-molecule array (Simoa), respectively. Both cohorts consisted of healthy controls (HC) and patients with cognitive decline of different etiologies. CSF SNAP-25 concentration was higher in AD and non-neurodegenerative diseases (i.e., vascular dementia) compared with controls but did not differ between AD and non-AD neurodegenerative diseases. We found a trend toward an association between SNAP-25 and disease burden when comparing HC, mild cognitive impairment due to AD, and AD. CSF SNAP-25 concentrations were strongly associated with CSF phosphorylated tau (p-tau) concentrations, thus strengthening the link between synaptic dysfunction and tau pathophysiology in AD. Our initial findings suggest that SNAP-25 may be a potential biomarker for differentiating AD from dementia due to other etiologies. However, due to the significant association between SNAP-25 and p-tau proteins, the clinical utility of SNAP-25 as a diagnostic biomarker for AD may be limited, while SNAP-25 may be useful for monitoring disease progression or treatment response.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 2","pages":"e70010"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11800177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Selective 5HT_2A Receptor Agonist, 25CN-NBOH Exerts Excitatory and Inhibitory Cellular Actions on Mouse Medial Prefrontal Cortical Neurons.","authors":"Yang Wang, Jesper L Kristensen, Kristi A Kohlmeier","doi":"10.1002/syn.70014","DOIUrl":"10.1002/syn.70014","url":null,"abstract":"<p><p>Psychedelic compounds have gained renewed interest due to their rapid and long-lasting therapeutic effects on stress-related disorders. While the underlying mechanisms of therapeutic actions of psychedelic compounds are still unclear, these drugs are thought to modulate the activity of the serotonergic system, primarily through activating serotonin type 2A receptor (5-HT_2AR) and studies have focused on these actions in the medial prefrontal cortex (mPFC). 25CN-NBOH, a synthetic psychedelic compound with a high binding affinity for 5-HT_2ARs and anti-anxiety actions, has emerged as a valuable tool for investigating the physiological functions mediated by this receptor. This study aimed to investigate the electrophysiological effects of 25CN-NBOH on pyramidal mPFC neurons using whole-cell patch clamp recordings in mouse brain slices. We recorded synaptic events and action potential rates during acute and long-term exposure to two concentrations of 25CN-NBOH. Acute application of 10 µM 25CN-NBOH increased the frequency of spontaneous excitatory postsynaptic currents (sEPSCs) that was reliant on activation of 5-HT_2AR, and which was not seen upon chronic exposure. A similar effect of 200 nM 25CN-NBOH was not noted. Surprisingly, both 10 µM and 200 nM 25CN-NBOH significantly suppressed the firing rate following acute as well as a longer-term exposure of 1 h. This suppression was independent of 5-HT_2AR activation but was mediated by M-current channels, as evidenced by the reversal of suppression with the M-current blocker XE-991. Our data suggest a complicated dual action of 25CN-NBOH in enhancing excitatory transmission while also reducing excitability. Our data contribute to knowledge regarding the cellular consequence of 5-HT_2AR agonism and contribute to widening our understanding of the potential mechanisms underlying the therapeutic actions of serotonergic psychedelics.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 2","pages":"e70014"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}