Stem Cell Reviews and Reports最新文献

{"title":"TNF-α Pathway Activation and Altered microRNA Expression in Peripheral Blood-Derived Endothelial Colony-Forming Cells: Insights into Diabetes-Induced Dysfunction.","authors":"Yaqiong Liu, Caomhán J Lyons, Alan Keane, Stuart McKeown, Edoardo Pedrini, Michael Creane, Nadeem Soomro, Alicja Straszewicz, Tomás Griffin, Alan Stitt, Timothy O'Brien","doi":"10.1007/s12015-025-10908-6","DOIUrl":"https://doi.org/10.1007/s12015-025-10908-6","url":null,"abstract":"","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-Derived Stem Cells at the Crossroads of Obesity and Cancer.","authors":"Ahmad Reza Panahi Meymandi, Abbas Ghaderi","doi":"10.1007/s12015-025-10918-4","DOIUrl":"https://doi.org/10.1007/s12015-025-10918-4","url":null,"abstract":"<p><p>Obesity is a major concern for public health and is associated with a higher risk of various types of cancer. Understanding the fundamental mechanisms of this relationship is essential for effective cancer prevention and treatment. Adipose-derived stem cells (ADSCs) are a specialized subtype of mesenchymal stem cells (MSCs) extracted from adipose tissue. These cells have the ability to undergo lineage-specific differentiation into adipocytes, osteoblasts, and chondrocytes. Although ADSCs have significant therapeutic potential, existing studies have indicated that they may facilitate cancer initiation, progression, and recurrence through multiple pathways, including their capacity to facilitate tumor cell proliferation, epithelial-to-mesenchymal transition (EMT), angiogenesis, formation of cancer stem cells (CSCs), immune evasion, and resistance to therapy. Obese individuals have a higher amount of adipose tissue containing an abundance of ADSCs compared to normal individuals, highlighting the possible role of ADSCs in the link between obesity and cancer. In this review, we have explored the effects of ADSCs on cancer initiation, promotion, and progression and put forward a hypothetical model to explain the elevated risk of cancer among obese patients.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144554913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Throughput Label-Free Continuous Quantification of Muscle Stem Cell Proliferation and Myogenic Differentiation.","authors":"Stig Skrivergaard, Martin Krøyer Rasmussen, Margrethe Therkildsen, Jette Feveile Young","doi":"10.1007/s12015-025-10915-7","DOIUrl":"https://doi.org/10.1007/s12015-025-10915-7","url":null,"abstract":"<p><strong>Background: </strong>Quantifying muscle satellite cell proliferation and differentiation is crucial for applications in muscle regeneration, disease modeling, and cultivated meat research. Traditional fluorescence-based assays, while sensitive, are labor-intensive, endpoint-restricted, and disruptive to myotube integrity.</p><p><strong>Methods: </strong>In this study, we present a novel high-contrast brightfield (HCBF) imaging technique for high-throughput, label-free assessment of both satellite cell proliferation and myogenic differentiation. Using the BioTek Cytation 5 automated imager and Gen5 software (Agilent Technologies), we optimized imaging parameters to achieve continuous, highly time-resolved quantification in standard 96- and 384-well formats without any additional reagents or cell manipulation needed.</p><p><strong>Results: </strong>Our approach enabled detailed kinetic profiling of satellite cell behavior, revealing myotube formation dynamics, species-specific media responses, optimal seeding conditions and the influence of mechanical factors on differentiation. We also demonstrated that serum-free media formulations could support efficient myotube formation in both bovine and porcine satellite cells, while having very different myotube kinetics and morphology than serum-containing samples. Furthermore, we highlighted the high degree of well-to-well variation and the sporadic formation and detachment of myotubes in culture, and the interesting phenomena of a second wave of myotubes being formed following detachment in serum-containing samples. Additionally, the 384-well format enabled a label-free screening method to assess clonal myogenicity of isolated satellite cells.</p><p><strong>Conclusion: </strong>By eliminating the need for genetic labeling, invasive staining or specialized consumables, our high-throughput HCBF methodology advances myogenic research, offering new opportunities for efficient screening and highly detailed kinetic data acquisition for serum-free media development, drug discovery and pathophysiological testing for both cultivated meat and musculoskeletal research.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144554914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Stem Cell Assisted Lipotransfer Approach for Breast Reconstruction in cancer Patients: Efficacy and Safety of the LIPO MULTI-SVF Study.","authors":"Francesco Agostini, Miriam Marangon, Stefania Zanolin, Marco Valvasori, Naike Casagrande, Martina Urbani, Elisabetta Lombardi, Valentina Visintini Cividin, Cristina Durante, Mario Mazzucato, Samuele Massarut","doi":"10.1007/s12015-025-10921-9","DOIUrl":"https://doi.org/10.1007/s12015-025-10921-9","url":null,"abstract":"","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144544949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Progerin in Artificially Ageing Stem Cells.","authors":"Rachel Brown, Mubeen Goolam","doi":"10.1007/s12015-025-10932-6","DOIUrl":"https://doi.org/10.1007/s12015-025-10932-6","url":null,"abstract":"","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144544950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induced Pluripotent Stem Cells Derived Cellular Models for Investigating Parkinson's Disease Pathogenesis and Drug Screening.","authors":"Jihong Liu, Wanlin Zhao, Zijuan Zhang, Xilei Ai, Bing Cao, Zhenqiang Zhang, Dongrui Ma","doi":"10.1007/s12015-025-10931-7","DOIUrl":"https://doi.org/10.1007/s12015-025-10931-7","url":null,"abstract":"<p><p>Parkinson's disease (PD) is the second most common neurodegenerative disorder, affecting millions worldwide. The complexity of PD has hindered the development of accurate disease models. Induced pluripotent stem cells (iPSCs), derived from patient-specific cells, offer a promising platform for modeling PD. This review discusses the development of PD models using iPSCs from different patient sources, focusing on 2D and 3D culture systems. We also explore the integration of iPSCs with advanced technologies like multi-omics, tissue engineering, and gene editing, and their potential to drive breakthroughs in disease modeling. Co-culture systems of iPSC-derived neurons and glial cells provide insights into cell-cell interactions in PD, while 3D brain region-specific organoids enhance understanding of interregional disease processes. Advances in multi-omics and gene editing have further propelled iPSC-based disease modeling, offering new avenues for investigating disease mechanisms and therapeutic screening.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct Lineage Reprogramming of Fibroblasts into Functional Keratinocyte-Like Cells via BMI1 and FGFR2b for Diabetic Wound Repair.","authors":"Hongrui Ma, Yashi Cao, Zizheng Gao, Zhifei Xu, Bo Yang, Peihua Luo, Yuhuai Hu, Qiaojun He","doi":"10.1007/s12015-025-10929-1","DOIUrl":"https://doi.org/10.1007/s12015-025-10929-1","url":null,"abstract":"<p><strong>Background: </strong>The limited regenerative capacity of epidermal cells following tissue injury impairs wound healing in diabetic foot ulcers (DFUs), contributing to elevated rates of amputation and mortality. Recent advances have demonstrated that somatic cells can be reprogrammed into diverse cell types through the application of defined reprogramming factors. This study aims to develop a safe, efficient, and clinically translatable strategy for skin regeneration via direct lineage reprogramming.</p><p><strong>Methods: </strong>We established a novel reprogramming approach using a combination of two factors, BMI1 and FGFR2b (termed B2), to induce fibroblast-to-keratinocyte-like cells (iKCs) conversion in vitro and delivered via adeno-associated virus 9 (AAV9) in vivo. Molecular and functional characteristics of iKCs were evaluated by qRT-PCR, Western blot, immunofluorescence, transcriptomic analysis, and in vitro differentiation assays. A diabetic (db/db) mouse skin wound model was used to assess the regenerative potential and therapeutic effects. Statistical significance was determined using Student's t-test or one-way ANOVA.</p><p><strong>Results: </strong>iKCs-B2 (Keratinocyte-like cells form from B2-infected L929) exhibited both morphological and functional characteristics comparable to primary keratinocytes. In vivo, AAV9-mediated delivery of B2 factors significantly promoted wound closure, reconstructed stratified epithelium, restored barrier function, and markedly reduced the mortality rate.</p><p><strong>Conclusions: </strong>This study presents a safe and effective direct reprogramming strategy for skin regeneration, bypassing the pluripotent stage and avoiding cell transplantation. The B2 combination provides a novel molecular tool for wound repair and offers translational potential for treating non-healing wounds such as DFUs.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic Study Revealed a Distinction Between Human Dermal Fibroblasts and Mesenchymal Stem Cells from Different Sources.","authors":"Slavomíra Nováková, Zuzana Hatoková, Maksym Danchenko, Gábor Beke, Ľuboš Kľučár, Lucia Slovinská, Denisa Harvanová, Peter Baráth, Ján Strnádel, Erika Halašová, Henrieta Škovierová","doi":"10.1007/s12015-025-10926-4","DOIUrl":"https://doi.org/10.1007/s12015-025-10926-4","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs) are an essential tool in cell-based therapies. One of the most crucial factors for efficacy in regenerative medicine is the source of MSCs. Tissue origin has long been suggested as a potential determinant of MSC properties. Human dermal fibroblasts (HDFa) share similar characteristics with MSCs, and the question of whether HDFa are functionally equivalent to MSCs remains debated. The present work used proteomic and phenotypic analyses to compare HDFa, dental pulp stem cells (DPSCs), and adipose-derived mesenchymal stem cells (AD-MSCs). We observed similarities and/or differences in morphology, cell surface markers, differentiation, and proteomic profile. Proteome was profiled by nano liquid chromatography and comprehensively quantified by mass spectrometry. In fact, HDFa and MSCs shared similar surface markers, growth kinetics, and differentiation capacity. Proteomic analysis reproducibly identified and quantified 3,051 proteins, 86 of them were differentially abundant according to strict statistical criteria. We identified a set of proteins that determined signatures for each stem cell origin. Gene Ontology (GO) term enrichment of differentially accumulated proteins, and Gene Set Enrichment Analysis (GSEA) identified signaling pathways characteristic to individual cell types. Particularly, we highlighted signaling pathways involved in cell migration, adhesion, and Wnt signaling as downregulated in HDFa compared to DPSCs. Angiogenesis and vascularization were explicitly associated with AD-MSCs. The tissue repair process requires a well-coordinated integration of complex molecular events, including cell migration and proliferation, extracellular matrix deposition, angiogenesis, and remodeling. We propose that HDFa are an alternative to MSCs, but predict their worse behavior in defect repair models compared to DPSCs. Plausibly, AD-MSCs are more suitable candidates for angiogenesis models compared to DPSCs.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rolipram and Electrical Stimulation Synergistically Promote Neuronal Differentiation of Adipose-derived Stromal Cells: an in Vitro Study.","authors":"Milad Rahimzadegan, Alireza Soltani Khaboushan, Somayeh Niknazar, Mohammadhosein Ghahremani, Hamid Akbari Javar, Omid Sabzevari, Zahra Hassannejad","doi":"10.1007/s12015-025-10925-5","DOIUrl":"https://doi.org/10.1007/s12015-025-10925-5","url":null,"abstract":"<p><p>This study investigates gold-decorated polycaprolactone/chitosan nanofibers as conductive scaffolds for promoting neuronal differentiation of adipose-derived mesenchymal stromal cells (ADSCs) harvested from the dorsal interscapular region of Wistar rats under electrical stimulation (ES) with optimal rolipram concentrations. The scaffold was fabricated through electrospinning and in situ synthesis of gold nanoparticles (AuNPs). Morphology and AuNPs distribution were evaluated using a Field Emission Scanning Electron Microscope (FE-SEM) and energy dispersive X-ray spectroscopy (EDX). Rolipram, known to increase neuronal cyclic adenosine monophosphate (cAMP) activity and reduce inflammation, was loaded into the scaffolds using alginate hydrogel. The scaffolds were subjected to a release study and tests for ADSC proliferation and differentiation into neuron-like cells. Immunostaining of β-Tubulin III and MAP2 was used to assess the effect of ES, alone and in combination with rolipram, on the efficacy of neuronal differentiation of ADSCs. The distribution of AuNPs was uniform within the scaffolds with an electrical conductivity of 0.12 S.cm^-1. Rolipram significantly improved the development of neurons from ADSCs, and this effect was more prominent at higher concentrations (1 and 5 µM). The study revealed that using an electrical density of 100 mV/mm, in combination with 5 µM rolipram and conductive scaffolds, led to a significant increase in the percentage of MAP2 and β-Tubulin III positive cells and the neuronal differentiation of ADSCs, with further elevation of cAMP levels compared to using 5 µM rolipram without ES. We found that combining rolipram and electrical stimulation at optimized doses and voltages can enhance nerve regeneration applications.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How Faithful Flt3-Cre Mouse is to Hematopoietic Lineage Tracing?","authors":"Jinglei Zhai, Xinying Li, Fang Dong, Hideo Ema","doi":"10.1007/s12015-025-10930-8","DOIUrl":"https://doi.org/10.1007/s12015-025-10930-8","url":null,"abstract":"<p><p>Flt3 gene expression is known to occur at a specific development stage of hematopoiesis in mice. Flt3-Cre reporter mice have been utilized in lineage tracing studies. This review systematically evaluates different Flt3-Cre reporter constructs, focusing on labeling efficiency and consistency with endogenous Flt3 expression patterns. We discuss the strengths and limitations associated with employing marker gene expression in lineage tracing. Furthermore, this lineage tracing strategy is compared with clonal tracing strategies such as barcoding and single-cell transplantation. Finally, we propose comparing hematopoietic stem cells identified by barcoding with those identified by transplantation to know the relationship between HSCs in non-stress and stress conditions. HIGHLIGHTS: • Flt3-Cre reporter mice are excellent models to understand hematopoiesis but may not necessarily reflect endogenous expression of Flt3. • The labeling efficiency significantly differs among the Flt3-Cre reporter mice.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}