Stem Cell Reviews and Reports最新文献

{"title":"Transcriptome-Wide Sequencing Identifies Non-Coding RNAs and Their Competing Endogenous RNA Networks During the Stages of pPGCLCs-Induced Differentiation.","authors":"Zhan-Zhong Qiao, Qiu-Yuan Pang, Chang Xu, Xin-Lei Feng, Ming-Xin Zang, Ping Wang, Ying Zhang, Yong-Chao Liu, Qian-Ru Han, Wei Shen, Wei Ge, Shun-Feng Cheng","doi":"10.1007/s12015-025-10902-y","DOIUrl":"https://doi.org/10.1007/s12015-025-10902-y","url":null,"abstract":"<p><p>Primordial germ cells (PGCs) are undifferentiated embryonic germ cells with the unique potential to develop into gametes. It is widely recognized that PGC development involves the activation of germ cell-specific genes and the repression of certain pluripotency genes. However, little is known about the noncoding RNAs that play a crucial role in regulating cellular functions during PGC development. In this study, to investigate the ncRNA regulatory network during PGC differentiation, whole transcriptome sequencing technology was employed during pPGCLC differentiation from porcine skin-derived stem cells (pSDSCs). Our findings unveiled that the TGF-β signaling pathway was indispensable for PGC cell fate commitment in pPGCLCs. Specifically, SMAD3 and ACVR2B, genes associated with the TGF-β pathway, showed marked upregulation at 20 d. We then identified their target microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), including ssc-miR-504, ssc-miR-125a, ssc-let-7c, MSTRG.8397, MSTRG.5581, MSTRG.4342, MSTRG.4186, and MSTRG.1058 and subsequently constructed the competitive endogenous RNA (ceRNA) network. To validate our analysis, we transfected miRNA inhibitors into cells. RT-qPCR analysis revealed a notable upregulation in the expression levels of MSTRG.8397, MSTRG.5581, MSTRG.4342, MSTRG.4186, MSTRG.1058, SMAD3, and ACVR2B compared to the negative control (NC) group. This is the first study to describe ncRNAs during the induction of pSDSCs to pPGCLCs. Our findings help to elucidate the molecular mechanism involved in the induction of pPGCLCs from pSDSCs.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LED-Modified Mesenchymal Stem Cells Improve Post-Infarction Heart Failure.","authors":"Carolina Fernanda Chaves Dos Santos, Ednei Luiz Antonio, Barbara Sampaio Dias Martins Mansano, Larissa Emília Seibt, Ighor Luiz Azevedo Teixeira, Helenita Antonia de Oliveira, Vitor Pocani da Rocha, Francisco Antonio Helfenstein Fonseca, Maria Cristina Izar, Adriano Mendes Caixeta, Andrey Jorge Serra","doi":"10.1007/s12015-025-10899-4","DOIUrl":"https://doi.org/10.1007/s12015-025-10899-4","url":null,"abstract":"","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Profiling of Trabecular Meshwork Progenitor Cells.","authors":"Xiaochen Fan, Stephanie Kennedy, Emine K Bilir, Brian Lane, Olivia A Kingston, Xu Chen, Victoria R Kearns, Colin E Willoughby, Carl M Sheridan","doi":"10.1007/s12015-025-10900-0","DOIUrl":"https://doi.org/10.1007/s12015-025-10900-0","url":null,"abstract":"<p><p>The loss and dysfunction of trabecular meshwork (TM) cells are implicated in aging and primary open-angle glaucoma. TM progenitor cells (TMPCs) contribute to the population and function of the TM, but their identity is not well elucidated. This study aimed to identify the expression profile of differentially expressed genes (DEGs) in human TM cell cultures, TM-derived spheres, and their differentiated progeny. Primary normal human TM cells (PTM) from three donors were cultured, de-differentiated into spheres, and re-differentiated into TM cells (DTM). RNA-Seq was performed using Illumina NGS, and bioinformatics analysis was conducted with Tuxedo, Bowtie2, Tophat, Cufflinks, and Ingenuity Pathway Analysis (IPA). DEGs were validated via Nanostring, RT-qPCR (in five independent donors), immunocytochemistry, and western blotting. RNA-seq identified significant DEGs in PTM, TM progenitor cells (TMPCs), and DTM cells. Gene expression in TMPCs differed significantly from PTM and DTM cells. Nanostring and RT-qPCR confirmed 70 DEGs upregulated in TMPCs (P < 0.05). Immunocytochemistry highlighted distinct markers in TMPCs (SOX2, NOTCH1, ANKG, MGP) versus PTM and DTM cells (TAGLN, TEM7, SPARC). Western blotting further analyzed MGP, TAGLN, and SPARC proteins, revealing significant upregulation of MGP in TMPCs and downregulation of TAGLN and SPARC in spheres compared to PTM cells. Pathway analysis revealed activation of cell cycle checkpoint regulation, SUMOylation, and STAT3 pathways in TMPCs, with HGF, MMP9, KDR, IGF1, and FOS as key node genes in TMPC development. RNA-Seq identified novel expression profile of potential TM markers and activated pathways in TMPCs, providing insights into TMPC behaviours in physiological and pathological conditions.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Intersection of Stem Cells and Nanomaterials: Implications for Tissue Engineering and Regenerative Medicine.","authors":"Chou-Yi Hsu, Mariam Ayad Abd, Mohammed Merza, Ali G Alkhathami, Suhas Ballal, Rishiv Kalia, A Sabarivani, Samir Sahoo, Hanen Mahmod Hulail","doi":"10.1007/s12015-025-10897-6","DOIUrl":"https://doi.org/10.1007/s12015-025-10897-6","url":null,"abstract":"<p><p>An emerging field with great implications for therapeutics and regenerative medicine is nanomaterial in stem cell biology. The aim of this review is to explore the potential of nanomaterials in stem cell research, covering targeted delivery, differentiation and tracking. We will also present safety aspects related to the use of nanomaterials, highlighting the need to perform full toxicity, biocompatibility, and long-term assessment of their implications for tissues and stem cells in the vicinity of the scaffold.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Cells to Exosomes: a Review of Non-Surgical Biotherapeutic-Based Strategies for Liver Regeneration in the Face of End-Stage Diseases.","authors":"Fatemeh Askari Yazdian, Matin Mojaveri Samak, Amirhossein Larijani, Mohammad Taghi Ashoobi, Mohadese Kharaqani, Mohammad Amin Ghezel, Zahra Barabadi, Elham Vojoudi","doi":"10.1007/s12015-025-10872-1","DOIUrl":"https://doi.org/10.1007/s12015-025-10872-1","url":null,"abstract":"<p><p>Liver diseases, such as hepatitis, cirrhosis, and liver cancer, pose significant public health challenges, ranking as the twelfth leading cause of death globally. Given the liver's critical functions in metabolism, detoxification, and biosynthesis, its impairment can lead to severe consequences, often resulting in end-stage liver failure. Although liver transplantation is regarded as the definitive intervention for advanced liver disease, factors such as a shortage of donors and potential surgical complications necessitate the investigation of non-surgical regenerative medicine alternatives. This manuscript provides a comprehensive review of innovative non-surgical therapies aimed at liver regeneration, with an emphasis on both cell-based and cell-free approaches. It examines the contributions of various stem cell populations, including mesenchymal stem cells, hematopoietic stem cells, and induced pluripotent stem cells, in facilitating liver repair through mechanisms of differentiation and paracrine signaling. Furthermore, it explores the therapeutic potential of exosomes and conditioned media derived from stem cells as biotherapeutic agents in the context of regenerative medicine. By elucidating the mechanisms that underpin liver regeneration, this study aspires to inform the development of effective therapeutic strategies to address liver diseases and slow their progression. By elucidating the underlying mechanisms of liver regeneration, this study aims to contribute to the development of effective therapeutic strategies to address liver diseases and slow their progression.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low Intensity Pulsed Ultrasounds Modulate Adipose Stem Cells Differentiation.","authors":"Tamara Fernández-Marcelo, Alba Calero, Beatriz de Lucas, María Garrido, Raúl L Arregui, Paulina Sury, Elena Quintana, Laura M Pérez, Elisa Fernández-Millán, Beatriz G Gálvez","doi":"10.1007/s12015-025-10896-7","DOIUrl":"https://doi.org/10.1007/s12015-025-10896-7","url":null,"abstract":"<p><p>LIPUS, low intensity pulsed ultrasounds, are considered a safe and non-invasive tool that have been used extensively in medicine for chronic diseases. We evaluated the effects produced by LIPUS on the physiological behaviour of mouse and human adipose stem cells (ASCs) as well as on adipose tissue explants. LIPUS stimulation for one minute did not affect mouse or human ASC proliferation or apoptosis, with no evident changes in morphology or cell growth. Further, RNA-seq analysis revealed more than 200 genes differentially expressed after ultrasound stimulation of mouse and human ASCs. Among them, the changes in gene expression mainly belong to the canonical pathways of stemness, energy metabolism or chemokine signalling. On the other hand, the migration ability of mouse and human ASCs was partially affected by the ultrasound protocol, slightly increasing their capacity to migrate, while maintaining their stemness properties. Major affection was detected on the adipocyte differentiation capacity. Indeed, LIPUS-treated ASCs were unable to differentiate into mature adipocytes and their inflammatory secretion profile was reduced in both mouse and human ASCs. These effects were confirmed on an ex vivo model of adipose tissue explants, demonstrating that LIPUS also provoked an anti-inflammatory profile in the adipose tissue while reducing adipocytes differentiation and lipids content. Overall, the strong adipogenesis blockade, the reduction of their secretion profile and the RNA-seq analysis suggest that LIPUS-treated ASCs may be prevented from differentiating into mature adipocytes and from exacerbating inflammation, thus limiting their contribution to obesity.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell and Synthetic Embryo Models: Advances, Applications, and Ethical Considerations.","authors":"Hany E Marei","doi":"10.1007/s12015-025-10890-z","DOIUrl":"https://doi.org/10.1007/s12015-025-10890-z","url":null,"abstract":"<p><p>Independent traditional gametes and recent advances in stem cell biology have made it possible to create synthetic embryo models (SEMs), altering our capacity to study early human development, congenital diseases, and regenerative medicine. By recreating key developmental events in vitro, these models provide unmatched insights into embryogenesis and provide creative platforms for disease modeling, drug discovery, and individualized therapy. The quick development in SEM research raises serious ethical, legal, and regulatory questions that call for creating transparent control systems. The methods applied in SEM fabrication, their biomedical applications, and the moral issues connected with their use are investigated in this review. We also look at future directions, including enhancing ethical frameworks, adding artificial intelligence, increasing model fidelity, and encouraging public participation. Through multidisciplinary cooperation, SEMs might address these problems and transform developmental biology, advancing ethical scientific advancement.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Latest Advanced Techniques for Improving Intestinal Organoids Limitations.","authors":"Lisa Tonini, Changhwan Ahn","doi":"10.1007/s12015-025-10894-9","DOIUrl":"https://doi.org/10.1007/s12015-025-10894-9","url":null,"abstract":"<p><p>Intestinal organoids are valuable tools across different disciplines, from a clinical aspect to the biomedical research, providing a unique perspective on the complexity of the gastrointestinal system. They are alternatives to common cell lines as they can offer insights into architectural functionality and reduce the use of animal models. A deeper understanding of their organoid characteristics is required to harness their full potential. Despite their beneficial uses and multiple advantages, organoids have limitations that remain unaddressed. This review aims to elucidate the principal limitations of intestinal organoids, investigate structural defects such as the deficiency in a vascularized and lymphatic system, and absence of the microbiome, restrictions in mimicking the physiological gut model, including the lack of an acid-neutralizing system or a shortage of digestive enzymes, and the difficulties in their long-term maintenance and polarity accessibility. Development of innovative techniques to address these limitations will lead to improve in vivo recapitulation and pioneering further advancements in this field.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering the Future of Regenerative Medicines in Gut Health with Stem Cell-Derived Intestinal Organoids.","authors":"Dinesh Kumar, Sonia Gupta, Vrinda Gupta, Rajni Tanwar, Anchal Chandel","doi":"10.1007/s12015-025-10893-w","DOIUrl":"https://doi.org/10.1007/s12015-025-10893-w","url":null,"abstract":"<p><p>The advent of intestinal organoids, three-dimensional structures derived from stem cells, has significantly advanced the field of biology by providing robust in vitro models that closely mimic the architecture and functionality of the human intestine. These organoids, generated from induced pluripotent stem cells (iPSCs), embryonic stem cells (ESCs), or adult stem cells, possess remarkable capabilities for self-renewal, differentiation into diverse intestinal cell types, and functional recapitulation of physiological processes, including nutrient absorption, epithelial barrier integrity, and host-microbe interactions. The utility of intestinal organoids has been extensively demonstrated in disease modeling, drug screening, and personalized medicine. Notable examples include iPSC-derived organoids, which have been effectively employed to model enteric infections, and ESC-derived organoids, which have provided critical insights into fetal intestinal development. Patient-derived organoids have emerged as powerful tools for investigating personalized therapeutics and regenerative interventions for conditions such as inflammatory bowel disease (IBD), cystic fibrosis, and colorectal cancer. Preclinical studies involving transplantation of human intestinal organoids into murine models have shown promising outcomes, including functional integration, epithelial restoration, and immune system interactions. Despite these advancements, several challenges persist, particularly in achieving reproducibility, scalability, and maturation of organoids, which hinder their widespread clinical translation. Addressing these limitations requires the establishment of standardized protocols for organoid generation, culture, storage, and analysis to ensure reproducibility and comparability of findings across studies. Nevertheless, intestinal organoids hold immense promise for transforming our understanding of gastrointestinal pathophysiology, enhancing drug development pipelines, and advancing personalized medicine. By bridging the gap between preclinical research and clinical applications, these organoids represent a paradigm shift in the exploration of novel therapeutic strategies and the investigation of gut-associated diseases.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying Immunomodulatory Subpopulations of Adipose Stromal Vascular Fraction and Stem/Stromal Cells Through Single-Cell Transcriptomics and Bulk Proteomics.","authors":"Adrienne M Parsons, Nagib Ahsan, Eric M Darling","doi":"10.1007/s12015-025-10889-6","DOIUrl":"https://doi.org/10.1007/s12015-025-10889-6","url":null,"abstract":"<p><p>A primary therapeutic characteristic of mesenchymal stem/stromal cells (MSCs) is their immunomodulatory activity. Adipose-derived stem/stromal cells (ASCs) are an abundant and easily isolated source of MSCs shown to have high immunosuppressive activity, making them attractive for therapy. Understanding the heterogeneous immunomodulatory potential of ASCs within the stromal vascular fraction (SVF) of adipose tissue could better inform treatment strategies. In this study, we integrate single-cell RNA sequencing (scRNA seq) with bulk proteomics to characterize subpopulations of SVF-derived ASCs that are phenotypically similar to cytokine-licensed, cultured ASCs. To better define the licensing process, we present scRNA seq and bulk proteomics data of cultured (P2) ASCs exposed to inflammatory cytokines, showing enrichment of pathways related to inflammation and apoptosis that positively correlate to the cytokine-mediated, trajectory-derived pseudotime. Using the Scissor algorithm, we integrate the proteomics data with uncultured (P0) SVF scRNA seq data, identifying an ASC subpopulation that is phenotypically like the cytokine-stimulated ASCs (Scissor-positive). Interactome analysis identifies Scissor-positive ASCs as stress adaptive immune regulators that function through IL6 and broad SEMA4 interactions and higher Visfatin signaling, while Scissor-negative ASCs show strong signatures of ECM remodeling through FN1 and immunosuppression through THY1 and MIF signaling. Our multimodal, integrative approach enabled identification of previously unidentified, distinct ASC subpopulations with differing immunomodulatory phenotypes that are present in, and can potentially be selected from, P0 SVF ASCs.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}