Stem Cell Reviews and Reports最新文献

{"title":"Gut Microbiome and its Impact on Outcomes following Hematopoietic Stem Cell Transplantation: a Comprehensive Review.","authors":"Babak Arjmand, Sana Badamchizadeh, Pouya Mehran, Maedeh Sarvari, Sepideh Alavi-Moghadam, Rasta Arjmand, Mostafa Rezaei-Tavirani, Ghasem Janbabaei, Mohamad Vaezi, Bagher Larijani","doi":"10.1007/s12015-025-10958-w","DOIUrl":"10.1007/s12015-025-10958-w","url":null,"abstract":"<p><p>Hematopoietic stem cell transplantation is an important treatment for hematological malignancy and disorders, but is fraught with high risks, including graft-versus-host disease, infection, and relapse. Recent evidence now identifies that the microbiome plays a significant role in influencing transplant outcomes, in which microbial dysbiosis-defined by reduced diversity and pathogen overgrowth-is linked to greater complications and death. Microbiome manipulation with approaches including beneficial microbial species, fiber, fecal transplants, and diet has the potential to mitigate these risks. Experiments show that the restoration of beneficial microbes can restore immunity, reduce graft-versus-host disease severity, and reduce infection. Some challenges remain, including standardization of protocols, long-term efficacy, and safety in immunocompromised recipients. Future research will be focused on mechanisms, trials, and new technology for microbiome-based therapy, with the ultimate goal of improving survival and quality of life for transplant recipients. Hereupon, this review addresses how microbiome engineering can revolutionize cancer treatment by optimizing gut microbial communities for better outcomes in hematopoietic stem cell transplantation (HSCT).</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2529-2547"},"PeriodicalIF":4.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping Hematopoietic Fate after Transplantation.","authors":"Stephanie N Hurwitz","doi":"10.1007/s12015-025-10946-0","DOIUrl":"10.1007/s12015-025-10946-0","url":null,"abstract":"<p><p>Hematopoietic stem and progenitor cells (HSPCs) form the foundation of lifelong blood cell production and immune function. Understanding their fate, including how they differentiate, self-renew, and respond to environmental cues has long been a cornerstone of stem cell biology and regenerative medicine. This knowledge is especially vital in the context of therapeutic hematopoietic stem and progenitor cell transplantation, where the diverse behavior of transplanted HSPCs directly impacts patient outcomes. Advances in single-cell omics, lineage barcoding, and in situ tracking now allow us to directly trace the developmental trajectories and clonal contributions of individual HSPCs. These tools are reshaping our understanding of hematopoiesis not as a rigid hierarchy but as a dynamic and adaptive system. This review highlights key technologies that enable fate mapping of HSPCs, integrates insights into clonal behavior during both transplantation and native hematopoiesis, and discusses how these findings are likely to inform future diagnostic and therapeutic strategies. CLINICAL TRIAL NUMBER: Not applicable.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2348-2360"},"PeriodicalIF":4.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12504396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144969724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological and Metabolomic Characterization of Human Dermal Fibroblasts and Mesenchymal Stem Cells Derived from Human Dental Pulp and Adipose Tissue: a Pilot Comparative Study.","authors":"Zuzana Hatoková, Bibiána Baďurová, Martin Kertys, Nela Žideková, Andrea Evinová, Lucia Kotúľová, Marián Grendár, Denisa Harvanová, Lucia Slovinská, Erika Halašová, Henrieta Škovierová, Slavomíra Nováková","doi":"10.1007/s12015-025-10970-0","DOIUrl":"10.1007/s12015-025-10970-0","url":null,"abstract":"<p><strong>Background: </strong>Several studies have suggested that adult human dermal fibroblasts (HDFa) may be a potential alternative source to mesenchymal stem cells for cell therapies. This study aims to characterize HDFa, adipose-derived stem cells (ADMSCs) and dental pulp stem cells (DPSCs) to investigate their proliferation, differentiation potential, mitochondrial respiration, and metabolomic profile. We identified molecules and characteristics that would differentiate MSCs from different sources or confirm their uniformity.</p><p><strong>Methods: </strong>Differentiation was induced using osteogenic and adipogenic differentiation media. Proteins specific to each differentiation process were monitored by immunofluorescence staining. High-resolution respirometry and targeted metabolomic analysis using the AbsoluteIDQ^®p180 kit (Biocrates Life Science) were applied to identify the essential properties of the studied cells.</p><p><strong>Results: </strong>HDFa cells, ADMSCs, and DPSCs demonstrated morphological characteristics of mesenchymal stem cells (MSCs). In general, DPSCs and HDFa showed significantly higher proliferation than ADMSCs. Osteogenic and adipogenic capacities were similar for all cell origins after 21 days, but ADMSCs exhibited earlier calcium deposit formation. FoxO1 and adiponectin as osteogenic and adipogenic-related proteins confirmed differentiation processes. High-resolution respirometry and metabolomic analysis showed potential distinguished characterization, mainly for DPSC cells. Our results also demonstrated that lipid profiling could be a promising tool for MSC characterization.</p><p><strong>Conclusions: </strong>Our analyzed data suggest that HDFa have properties similar to DPSCs and ADMSCs. However, each cell type has been shown to have unique specific characteristics. The similarities and differences in the characteristics of HDFa, ADMSCs, and DPSCs should be studied in detail and in a larger cohort when planning stem cell-based therapy.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2727-2744"},"PeriodicalIF":4.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12504344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Timosaponin B- II Enhances Osteogenic Differentiation of Human Periodontal Ligament Stem Cells via PI3K/AKT/GSK3β Signaling Pathway.","authors":"Dai-Wei Yang, Xiao-Bin Cui, Rui Dong, Zhao-Yan Wu, Yu-Xing Zhang, Peng Yang, Jun Zhang","doi":"10.1007/s12015-025-10962-0","DOIUrl":"10.1007/s12015-025-10962-0","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;This investigation aims to elucidate the effects of Timosaponin B-II (TB-II) on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) through both in vitro experiments and an in vivo orthodontic tooth movement model utilizing rats. The primary objective is to clarify the mechanisms by which TB-II influences the remodeling of periodontal tissue under biomechanical stress, thereby providing insights into its potential role in reducing relapses after orthodontic tooth movement.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;hPDLSCs were isolated and characterized via flow cytometry and multilineage differentiation assays (osteogenic and adipogenic induction). The impact of TB-II on the expression levels of osteogenic genes and proteins, including runt-related transcription factor-2 (RUNX-2), alkaline phosphatase (ALP), and collagen type 1 (COL-1), was evaluated through quantitative real-time PCR (qRT-PCR) and Western blotting. Alizarin Red Staining (ARS) was utilized to assess the formation of mineralized nodules. Additionally, the involvement of the phosphatidylinositol - 3 - kinase (PI3K)/ protein kinase B(AKT)/ glycogen synthase kinase - 3β(GSK3β) signaling pathway in TB-II-mediated osteogenesis was explored using pharmacological inhibitors (LY294002 for PI3K/AKT and CHIR-99021 for GSK3β). Western blot analysis identified key osteogenic markers (GSK3β, p-GSK3β, AKT, p-AKT) in treated cells. For in vivo validation, eighteen male Wistar rats were randomly divided into TB-II-treated and saline-control groups. Micro-computed tomography (micro-CT) evaluated tooth movement and alveolar bone structural changes. Histological assessment included hematoxylin-eosin (HE) staining, Masson trichrome staining, and tartaric-resistant acid phosphatase (TRAP) staining to analyze periodontal tissue morphology. Immunohistochemical (IHC) analysis assessed osteogenic markers (RUNX-2, ALP, COL-1) and the osteoclastogenic regulator RANKL to evaluate tissue remodeling. All statistical analyses were performed using GraphPad Prism 8. Comparisons between groups were conducted via one-way/two-way ANOVA with Tukey's post-hoc test. Values of p &lt; 0.05 were regarded as statistically significant.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In vitro studies revealed that TB-II at 20 μM significantly enhanced the proliferation, ALP activity, and mineralized nodule formation of hPDLSCs, accompanied by markedly elevated expression of RUNX-2, ALP, COL-1, p-AKT and p-GSK3β. Pharmacological inhibition of the PI3K/AKT pathway via LY294002 abolished TB-II's osteogenic effects, while treatment with CHIR99021 indicated that GSK3β activity was downstream and regulated by the PI3K/AKT signaling axis. In vivo, TB-II administration in a rat orthodontic tooth movement (OTM) model upregulated RUNX-2, ALP, and COL-1 expression on the tension side of tooth roots, while simultaneously reducing TRAP + osteoclast numbers and inhibiting RANKL expressio","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2675-2692"},"PeriodicalIF":4.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12504409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144969670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulated miR-199a-3p in Preeclamptic Placenta-derived Exosomes from Cord Blood Hinders VEGF-induced Fetal Glomerular Dysplasia Through Inhibiting Akt1(S473) Phosphorylation via Targeting PHLPP2.","authors":"Xiaotong Jiang, Mengqi Gu, Yu Xie, Yuchen Li, Qingfeng Lv, Pengzheng Chen, Die Jing, Yu Zhou, Xietong Wang, Lei Li","doi":"10.1007/s12015-025-10935-3","DOIUrl":"10.1007/s12015-025-10935-3","url":null,"abstract":"<p><strong>Background: </strong>Preeclampsia is amultisystem disorder involving in inflammatory responses and metabolic dysfunction of maternal-fetal circulation. Recently, researchers found it threatens renal health of offspring in adulthood. Growing evidence indicated chronic kidney disease is associated with glomeruli deficiencies during intrauterine development. Our previous study showed placenta-derived exosomes from cord plasma with preeclampsia impede fetal glomerular vascularization, during which we postulate microRNAs may function as epigenetic switches for gene silencing of human glomerular endothelial cells. However, the specific miRNAs in placenta-derived exosomes engaged in glomerular vascularization remain unclear.</p><p><strong>Methods: </strong>Small RNA sequencing of placental-derived exosomes and bioinformatics analysis were applied to identify differentially expressed miRNAs, followed by real-time polymerase chain reaction for verification. Transient expression and inhibition of candidate miRNA were performed by transfection with chemically synthesized miRNA oligonucleotides. Functional assays of HGECs including cell proliferation assays, EDU assays, migration assays, tube formation assays and monolayer cell barrier permeability assays were performed after transfection. Further, dual luciferase assay was used to explore the target genes of candidate miRNA, followed by RT-qPCR, western blot and rescue assays. Antagomirs transfection of C57BL/6 J fetal mice via Amniotic cavity injection in vivo and C57BL/6 J fetal mice kidney explants culture in vitro were performed to evaluate number of glomeruli, renal development.</p><p><strong>Results: </strong>Preeclampsia downregulates miR-199a-3p in placenta-derived exosomes from cord plasma. Suppression of endogenous miR-199a-3p in HGECs inhibits angiogenesis, proliferation, migration and permeability. A dual luciferase assay and rescue assays confirmed that miR-199a-3p targets PH domain leucine-rich repeat-containing protein phosphatase 2, regulating the phosphorylation of Akt serine/threonine kinase 1 (S473). C57BL/6 J fetal mice with miR-199a-3p downregulation have low glomerulus counts and relative growth rate.</p><p><strong>Conclusions: </strong>miR-199a-3p in placenta-derived exosomes from cord plasma controls VEGF-induced glomerular angiogenesis. Moreover, it provides a distinct perspective for the mechanisms underlying the increased risk for renal disease in the offspring of preeclampsia patients via placenta-derived exosomal miRNAs.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2693-2710"},"PeriodicalIF":4.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144969655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in Chitosan and Cellulose Nanoparticles for Stem Cell-Based Tissue Engineering.","authors":"Zafar Aminov, Harikumar Pallathadka, Muthena Kareem, Lalji Baldaniya, Dmitry Olegovich Bokov, Mamata Chahar, Suman Saini, Ish Kapila, Yasser Fakri Mustafa, Israa Habeeb Naser","doi":"10.1007/s12015-025-10960-2","DOIUrl":"10.1007/s12015-025-10960-2","url":null,"abstract":"<p><p>Stem cell-based tissue engineering offers transformative solutions for regenerating damaged tissues, such as bone, cartilage, and neural tissues. Chitosan and cellulose nanoparticles have emerged as promising biomaterials for enhancing stem cell delivery and scaffold performance due to their biocompatibility, biodegradability, and tunable properties. Chitosan, with its antimicrobial and bioadhesive properties, supports stem cell adhesion and differentiation in soft tissue scaffolds. Cellulose nanoparticles, including cellulose nanocrystals (CNCs), nanofibrils (CNFs), and bacterial nanocellulose (BNC), provide mechanical strength for hard tissue regeneration. This review examines their synthesis, properties, and interactions with stem cells, particularly mesenchymal stem cells (MSCs), in tissue engineering applications. A comparative analysis highlights their complementary roles, while challenges like physiological stability and scalability are addressed. Recent advancements, such as 3D bioprinting and growth factor functionalization, enhance their potential. Future research should focus on optimizing stem cell-scaffold interactions and ensuring clinical safety.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2511-2528"},"PeriodicalIF":4.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organoids in Genetic Disorders: from Disease Modeling to Translational Applications.","authors":"Yuanhang Zhu, Nanshan Lin, Juan Li, Haoqian Zhang, Ping Zhang, Xin Cheng, Qian Yang, Ling Liu","doi":"10.1007/s12015-025-10973-x","DOIUrl":"10.1007/s12015-025-10973-x","url":null,"abstract":"<p><p>The emergence of organoid models has significantly bridged the gap between traditional cell cultures/animal models and authentic human disease states, particularly for genetic disorders, where their inherent genetic fidelity enables more biologically relevant research directions and enhances translational validity. This review systematically analyzes established organoid models of genetic diseases across organs (e.g., brain, eye, kidney, lung, and heart), highlighting their pivotal roles in identifying novel pathogenic genes, elucidating disease mechanisms, and advancing therapeutic strategies such as drug screening platforms, gene-editing therapies, and organ transplantation strategies. Furthermore, we critically address current limitations-including challenges in recapitulating complex pathologies and scaling production-while underscoring their potential for personalized medicine through multi-omics integration and bioengineering innovations. Although the scope of \"genetic diseases\" is broad, this synthesis focuses on disorders with well-defined inheritance patterns, such as monogenic disorders, copy number variations (CNVs), and aneuploidies. Despite covering only a subset of these conditions, this review aims to provide researchers with a comprehensive overview of the field, emphasizing how organoid-based approaches could accelerate both mechanistic discoveries and clinical translation in genetic disease research.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2578-2596"},"PeriodicalIF":4.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How Faithful Flt3-Cre Mouse is to Hematopoietic Lineage Tracing?","authors":"Jinglei Zhai, Xinying Li, Fang Dong, Hideo Ema","doi":"10.1007/s12015-025-10930-8","DOIUrl":"10.1007/s12015-025-10930-8","url":null,"abstract":"<p><p>Flt3 gene expression is known to occur at a specific development stage of hematopoiesis in mice. Flt3-Cre reporter mice have been utilized in lineage tracing studies. This review systematically evaluates different Flt3-Cre reporter constructs, focusing on labeling efficiency and consistency with endogenous Flt3 expression patterns. We discuss the strengths and limitations associated with employing marker gene expression in lineage tracing. Furthermore, this lineage tracing strategy is compared with clonal tracing strategies such as barcoding and single-cell transplantation. Finally, we propose comparing hematopoietic stem cells identified by barcoding with those identified by transplantation to know the relationship between HSCs in non-stress and stress conditions. HIGHLIGHTS: • Flt3-Cre reporter mice are excellent models to understand hematopoiesis but may not necessarily reflect endogenous expression of Flt3. • The labeling efficiency significantly differs among the Flt3-Cre reporter mice.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2361-2371"},"PeriodicalIF":4.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a High-throughput Morphological Assay for Evaluating Mesenchymal Stromal Cell-derived Extracellular Vesicle Modulation of Brain Pericyte Secretory Phenotype.","authors":"Courtney E Campagna, Andrew M Larey, Kanupriya R Daga, Morgan Roos, Sneha Ghosh, Neil Grimsey, Jin Han, Ross A Marklein","doi":"10.1007/s12015-025-10940-6","DOIUrl":"10.1007/s12015-025-10940-6","url":null,"abstract":"<p><p>Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) are a promising therapeutic tool for treating many neurodegenerative diseases. Neuroinflammation plays a major role in many of these conditions through an orchestration of interdependent processes that lead to the breakdown of the blood-brain barrier (BBB), infiltration of immune cells and neuronal death. MSC-EVs have shown preliminary evidence of modulating neuroinflammation, but their mechanisms of action are still unknown. Therefore, we explored the potential of MSC-EVs in modulating brain pericytes, a cell type that plays a critical role in BBB maintenance but has not been investigated as a therapeutic target for MSC-EVs. Brain pericytes are multifaceted cells that can modulate neuroinflammation through their involvement in BBB homeostasis, as well as the innate and adaptive immune response. Pericyte morphology has been shown to change in response to inflammatory stimuli in vivo, hence, we used this behavior to develop a quantitative morphological profiling approach to assess the immunomodulatory function of MSC-EVs in a high-throughput, low-cost manner. Using this assay, we were able to demonstrate that MSC-EVs manufactured under various conditions (2D, 3D, and in response to cytokine priming) could induce distinct pericyte morphological responses indicative of changes in secretion of chemokines and cytokines relevant to neuroinflammation.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2781-2795"},"PeriodicalIF":4.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12504408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanovesicles from Adipose Derived Stem Cells Delay Hair Follicle Degeneration and Stimulate Regeneration: a Novel Therapeutic Strategy for Androgenetic Alopecia.","authors":"Songjia Tang, Xiaoxin Wu, Yuyan Wang, Wei Zhang, Jie Wang, Jufang Zhang, Jinsheng Li, Chunmao Han","doi":"10.1007/s12015-025-10953-1","DOIUrl":"10.1007/s12015-025-10953-1","url":null,"abstract":"<p><strong>Background: </strong>Androgenetic alopecia has emerged as a significant medical and social concern. Current therapeutic options are limited, necessitating the exploration of more effective treatments. This study aimed to evaluate the efficacy of adipose derived stem cells nanovesicles (ADSC-NV) in treating androgenetic alopecia.</p><p><strong>Results: </strong>ADSC-NV presented as biconcave disc-shaped entities, with a mean diameter of 87.3 ± 36.5 nm. Nanovesicles significantly promoted the proliferation and migration of DPC, with a concentration of 60µg/mL demonstrating superior efficacy compared to 30 µg/mL. Besides, nanovesicles were found to slow the degeneration of hair follicles in androgenetic alopecia during in vitro culture. Intradermal injection of nanovesicles markedly enhanced hair regeneration in the dorsal skin of mice in vivo. Histological examination revealed that nanovesicles reversed hair follicle miniaturization and accelerated the transition of hair follicles from the telogen phase to the anagen phase. Notably, nanovesicles achieved an effect comparable to minoxidil, while also reducing sebum secretion.</p><p><strong>Conclusion: </strong>This study demonstrated the therapeutic potential of ADSC-NV in treating androgenetic alopecia both in vitro and in vivo. This finding provides a promising therapeutic strategy for addressing androgenetic alopecia.</p>","PeriodicalId":21955,"journal":{"name":"Stem Cell Reviews and Reports","volume":" ","pages":"2671-2674"},"PeriodicalIF":4.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144795531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}