来自人类牙髓和脂肪组织的人真皮成纤维细胞和间充质干细胞的生物学和代谢组学特征：一项初步比较研究。

IF 4.2 3区医学 Q2 CELL & TISSUE ENGINEERING

Stem Cell Reviews and Reports Pub Date : 2025-11-01 Epub Date: 2025-09-10 DOI:10.1007/s12015-025-10970-0

Zuzana Hatoková, Bibiána Baďurová, Martin Kertys, Nela Žideková, Andrea Evinová, Lucia Kotúľová, Marián Grendár, Denisa Harvanová, Lucia Slovinská, Erika Halašová, Henrieta Škovierová, Slavomíra Nováková

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引用次数: 0

摘要

背景：一些研究表明，成人真皮成纤维细胞（HDFa）可能是细胞治疗中间充质干细胞的潜在替代来源。本研究旨在表征HDFa、脂肪来源干细胞（ADMSCs）和牙髓干细胞（DPSCs），以研究它们的增殖、分化潜力、线粒体呼吸和代谢组学特征。我们确定了分子和特征，可以区分来自不同来源的间充质干细胞或确认其一致性。方法：采用成骨和成脂培养基诱导分化。免疫荧光染色检测各分化过程的特异性蛋白。使用AbsoluteIDQ®p180试剂盒（Biocrates Life Science）进行高分辨率呼吸测量和靶向代谢组学分析，以确定所研究细胞的基本特性。结果：HDFa细胞、ADMSCs和DPSCs表现出间充质干细胞（MSCs）的形态学特征。总的来说，DPSCs和HDFa的增殖明显高于ADMSCs。21天后，所有细胞来源的成骨和成脂能力相似，但ADMSCs表现出更早的钙沉积形成。fox01和脂联素作为成骨和成脂相关蛋白证实了分化过程。高分辨率呼吸测量和代谢组学分析显示了潜在的区分特征，主要针对DPSC细胞。我们的研究结果还表明，脂质谱分析可能是一种很有前途的MSC表征工具。结论：我们分析的数据表明HDFa具有与DPSCs和ADMSCs相似的特性。然而，每一种细胞类型都有其独特的特性。在计划干细胞治疗时，应在更大的队列中详细研究HDFa、ADMSCs和DPSCs特征的异同。

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Biological and Metabolomic Characterization of Human Dermal Fibroblasts and Mesenchymal Stem Cells Derived from Human Dental Pulp and Adipose Tissue: a Pilot Comparative Study.

Background: Several studies have suggested that adult human dermal fibroblasts (HDFa) may be a potential alternative source to mesenchymal stem cells for cell therapies. This study aims to characterize HDFa, adipose-derived stem cells (ADMSCs) and dental pulp stem cells (DPSCs) to investigate their proliferation, differentiation potential, mitochondrial respiration, and metabolomic profile. We identified molecules and characteristics that would differentiate MSCs from different sources or confirm their uniformity.

Methods: Differentiation was induced using osteogenic and adipogenic differentiation media. Proteins specific to each differentiation process were monitored by immunofluorescence staining. High-resolution respirometry and targeted metabolomic analysis using the AbsoluteIDQ^®p180 kit (Biocrates Life Science) were applied to identify the essential properties of the studied cells.

Results: HDFa cells, ADMSCs, and DPSCs demonstrated morphological characteristics of mesenchymal stem cells (MSCs). In general, DPSCs and HDFa showed significantly higher proliferation than ADMSCs. Osteogenic and adipogenic capacities were similar for all cell origins after 21 days, but ADMSCs exhibited earlier calcium deposit formation. FoxO1 and adiponectin as osteogenic and adipogenic-related proteins confirmed differentiation processes. High-resolution respirometry and metabolomic analysis showed potential distinguished characterization, mainly for DPSC cells. Our results also demonstrated that lipid profiling could be a promising tool for MSC characterization.

Conclusions: Our analyzed data suggest that HDFa have properties similar to DPSCs and ADMSCs. However, each cell type has been shown to have unique specific characteristics. The similarities and differences in the characteristics of HDFa, ADMSCs, and DPSCs should be studied in detail and in a larger cohort when planning stem cell-based therapy.

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来源期刊

Stem Cell Reviews and Reports 医学-细胞生物学

CiteScore

9.30

自引率

4.20%

发文量

审稿时长

3 months

期刊介绍： The purpose of Stem Cell Reviews and Reports is to cover contemporary and emerging areas in stem cell research and regenerative medicine. The journal will consider for publication: i) solicited or unsolicited reviews of topical areas of stem cell biology that highlight, critique and synthesize recent important findings in the field. ii) full length and short reports presenting original experimental work. iii) translational stem cell studies describing results of clinical trials using stem cells as therapeutics. iv) papers focused on diseases of stem cells. v) hypothesis and commentary articles as opinion-based pieces in which authors can propose a new theory, interpretation of a controversial area in stem cell biology, or a stem cell biology question or paradigm. These articles contain more speculation than reviews, but they should be based on solid rationale. vi) protocols as peer-reviewed procedures that provide step-by-step descriptions, outlined in sufficient detail, so that both experts and novices can apply them to their own research. vii) letters to the editor and correspondence. In order to facilitate this exchange of scientific information and exciting novel ideas, the journal has created five thematic sections, focusing on: i) the role of adult stem cells in tissue regeneration; ii) progress in research on induced pluripotent stem cells, embryonic stem cells and mechanism governing embryogenesis and tissue development; iii) the role of microenvironment and extracellular microvesicles in directing the fate of stem cells; iv) mechanisms of stem cell trafficking, stem cell mobilization and homing with special emphasis on hematopoiesis; v) the role of stem cells in aging processes and cancerogenesis.