Stem cell investigation最新文献_第10页

Periostin expression and characters of human adipose tissue-derived mesenchymal stromal cells were aberrantly affected by in vitro cultivation. 体外培养对人脂肪组织来源的间充质基质细胞Periostin的表达和特性产生了异常影响。

Stem cell investigation Pub Date : 2019-09-23 DOI: 10.21037/sci.2019.08.09

Heba M. Saad Eldien, H. Abdel-Aziz, D. Sayed, W. Mubarak, H. H. Hareedy, S. Mansor, Toshiko Yoshida, M. Fathy

{"title":"Periostin expression and characters of human adipose tissue-derived mesenchymal stromal cells were aberrantly affected by in vitro cultivation.","authors":"Heba M. Saad Eldien, H. Abdel-Aziz, D. Sayed, W. Mubarak, H. H. Hareedy, S. Mansor, Toshiko Yoshida, M. Fathy","doi":"10.21037/sci.2019.08.09","DOIUrl":"https://doi.org/10.21037/sci.2019.08.09","url":null,"abstract":"Background\u0000Human adipose tissue-derived mesenchymal stromal cells (AD-MSCs) have been under focus in regenerative medicine since their discovery as a suitable source of MSCs. AD-MSCs are heterogeneous cells and exhibit variations in population doubling time, morphology and proliferative capacity. This study investigated if human AD-MSCs are developing, during in vitro long-term cultivation, in an unwanted or aberrant way.\u0000\u0000\u0000Methods\u0000This study monitored AD-MSCs during their in vitro culture till the tenth passage investigating proliferation kinetics, DNA index and surface markers expression. Also, periostin gene expression was examined.\u0000\u0000\u0000Results\u0000The proliferation capacity and colony forming unit were decreased after passage 6 and the population doubling time was increased. Flow cytometric analysis revealed that newly cultivated population strongly expressed MSCs markers, furthermore, reduction of CD105 expression appeared in passage 5 onwards, the later was associated with significant increase in expression of CD34 (a hematopoietic cell marker). Also, reduction of CD73 and CD90 expression was observed from passage 8. Furthermore, during the first six passages, periostin expression was significantly unchanged, with significant upregulation in late passages.\u0000\u0000\u0000Conclusions\u0000Long-term cultivation of human AD-MSCs changed their characters in an aberrant way and the first four passages might be the most appropriate passages for therapy. More investigation and understanding of these variations are needed to help in standardizing the expansion of MSCs-based therapies.","PeriodicalId":21938,"journal":{"name":"Stem cell investigation","volume":"6 1","pages":"33"},"PeriodicalIF":0.0,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21037/sci.2019.08.09","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42069949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

CRISPR-activation-based screen reveals neuronal fate promotion by polycomb repressive complex 2 during direct reprogramming. 基于crispr激活的筛选揭示了在直接重编程过程中多梳抑制复合体2对神经元命运的促进作用。

Stem cell investigation Pub Date : 2019-09-12 DOI: 10.21037/sci.2019.08.04

Tim Wolfram, B. Tursun

引用次数: 0

Stem Cell Ophthalmology Treatment Study (SCOTS): bone marrow derived stem cells in the treatment of Usher syndrome. 干细胞眼科治疗研究（SCOTS）：骨髓来源的干细胞治疗Usher综合征。

Stem cell investigation Pub Date : 2019-09-09 DOI: 10.21037/sci.2019.08.07

J. Weiss, S. Lévy

{"title":"Stem Cell Ophthalmology Treatment Study (SCOTS): bone marrow derived stem cells in the treatment of Usher syndrome.","authors":"J. Weiss, S. Lévy","doi":"10.21037/sci.2019.08.07","DOIUrl":"https://doi.org/10.21037/sci.2019.08.07","url":null,"abstract":"Background\u0000Usher syndrome is the most common form of syndromic retinitis pigmentosa and includes types I, II, and III with varying degrees of hearing loss. We present results of 10 eyes with Usher syndrome treated with autologous bone marrow derived stem cells (BMSC) within the Stem Cell Ophthalmology Treatment Study (SCOTS).\u0000\u0000\u0000Methods\u0000Preoperative Snellen visual acuities ranged from 20/30-1 to 20/400 with the average pre-operative Snellen acuity approximately 20/85 and the average logarithm of the minimum angle of resolution (LogMAR) acuity 0.635. All eyes had significantly impaired visual fields and patients reported hearing loss as part of this syndromic retinitis pigmentosa. Treatment using the protocols of the SCOTS study using BMSC provided by retrobulbar, subtenons, intravitreal and intravenous injections.\u0000\u0000\u0000Results\u0000Following treatment, 80% of the Usher eyes showed an improvement in visual acuity. Of the eyes that improved the average increase in visual acuity was 36.4% on LogMAR with improvements ranging from 23% to 94%. The average post-operative change in all treated eyes was a gain of 0.18 LogMAR and an increase in visual acuity of 28.3% on LogMAR. The results showed high statistical significance with P<0.001. Visual fields generally improved. No patient experienced a loss of vision. One patient underwent preoperative and 4-month post-operative audiometry testing which demonstrated improvement. The procedures were performed safely and without complications.\u0000\u0000\u0000Conclusions\u0000Findings confirm meaningful improvement in visual acuity is possible in Usher syndrome using BMSC protocols developed in the SCOTS study. Statistical significance and safety were established.","PeriodicalId":21938,"journal":{"name":"Stem cell investigation","volume":"6 1","pages":"31"},"PeriodicalIF":0.0,"publicationDate":"2019-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21037/sci.2019.08.07","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45479147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Seeking fate-CRISPRa screens reveal new neural lineage and reprogramming factors. 寻求命运- crispra筛选揭示新的神经谱系和重编程因素。

Stem cell investigation Pub Date : 2019-09-09 DOI: 10.21037/sci.2019.08.03

Valentin Baumann, S. Stricker

引用次数: 1

SETD7 in cardiomyocyte differentiation and cardiac function. SETD7在心肌细胞分化和心功能中的作用。

Stem cell investigation Pub Date : 2019-09-09 DOI: 10.21037/sci.2019.08.01

T. Basuroy, I. L. de la Serna

引用次数: 1

Philadelphia-positive lymphoblastic lymphoma: a case report and review of the literature. 费城阳性淋巴母细胞淋巴瘤：一例报告并文献复习。

Stem cell investigation Pub Date : 2019-09-07 DOI: 10.21037/SCI.2019.06.06

M. Dragani, G. Andreani, C. Fava, F. Daraio, E. Gottardi, E. Giugliano, P. Nicoli, G. Rege‐Cambrin

引用次数: 5

Spontaneous tumor lysis syndrome in T-cell malignancy: two case reports. T细胞恶性肿瘤中的自发性肿瘤溶解综合征：两例报告。

Stem cell investigation Pub Date : 2019-08-20 DOI: 10.21037/SCI.2019.07.01

W. Roque, A. Rehman, G. Suero-Abreu, B. Danek, Joseph Colao, Alla Fayngersh, S. Srinivas, J. Kra, D. Cai, V. Chang

引用次数: 5

Epithelial-mesenchymal plasticity-engaging stemness in an interplay of phenotypes. 表型相互作用中的上皮-间充质可塑性参与干性。

Stem cell investigation Pub Date : 2019-08-20 DOI: 10.21037/SCI.2019.08.08

V. Chin, C. Lim

引用次数: 25

Isolating human dermal fibroblasts using serial explant culture. 使用连续外植体培养分离人真皮成纤维细胞。

Stem cell investigation Pub Date : 2019-08-20 DOI: 10.21037/SCI.2019.08.05

Fereshte Nejaddehbashi, V. Bayati, L. Mashali, M. Hashemitabar, M. Abbaspour, E. Moghimipour, M. Orazizadeh

{"title":"Isolating human dermal fibroblasts using serial explant culture.","authors":"Fereshte Nejaddehbashi, V. Bayati, L. Mashali, M. Hashemitabar, M. Abbaspour, E. Moghimipour, M. Orazizadeh","doi":"10.21037/SCI.2019.08.05","DOIUrl":"https://doi.org/10.21037/SCI.2019.08.05","url":null,"abstract":"Background\u0000The purpose of this study was to introduce an applicable culture technique to isolate human dermal fibroblasts (HDFs); which could also contribute to research, clinical practices, as well as tissue engineering.\u0000\u0000\u0000Methods\u0000Samples from the human skin were dissected and cultured via serial explant technique. Subsequently, the isolated fibroblasts were assessed for their protein markers and genetic variations via immunofluorescence (IF) and karyotyping; respectively. Following the employment of this technique, a small piece of explant completely disappeared; while no dermis remained after 10 days.\u0000\u0000\u0000Results\u0000The quantity of HDFs harvested through this culture technique was reported at a normal level. The results of immunostaining also indicated that the isolated fibroblasts had expressed vimentin and fibronectin; whereas no cells had shown cytokeratin and epidermal marker. Moreover, karyotyping results for the fibroblasts isolated by the given technique revealed no chromosomal diversity after passage 20.\u0000\u0000\u0000Conclusions\u0000It was concluded that serial explant culture was an efficient technique for isolating HDFs from a small piece of skin in short-time periods; which could also preserve their normal morphology and molecular characteristics.","PeriodicalId":21938,"journal":{"name":"Stem cell investigation","volume":"6 1","pages":"23"},"PeriodicalIF":0.0,"publicationDate":"2019-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21037/SCI.2019.08.05","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42880446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Cell-based therapy for idiopathic pulmonary fibrosis. 特发性肺纤维化的细胞治疗。

Stem cell investigation Pub Date : 2019-08-16 eCollection Date: 2019-01-01 DOI: 10.21037/sci.2019.06.09

Qi Lu, Ahmed H K El-Hashash

{"title":"Cell-based therapy for idiopathic pulmonary fibrosis.","authors":"Qi Lu, Ahmed H K El-Hashash","doi":"10.21037/sci.2019.06.09","DOIUrl":"10.21037/sci.2019.06.09","url":null,"abstract":"<p><p>Idiopathic pulmonary fibrosis (IPF) is an example of interstitial lung diseases that is characterized by chronic, progressive, and fibrotic lung injuries. During lung fibrosis, normal healthy lung tissues are replaced by remarkably destroyed alveolar architecture and altered extracellular cell matrix. These changes eventually cause severe disruption of the tightly-controlled gas exchange process and reduction of lung compliance that ultimately lead to both respiratory failure and death. In the last decade, progress has been made toward understanding the pathogenesis of pulmonary fibrosis, and two novel disease-modifying therapies were approved. However, finding more effective treatments for pulmonary fibrosis is still a challenge, with its incidence continues to increase globally, which is associated with significantly high mortality, morbidity and economical healthcare burden. Different stem cell types have recently emerged as a promising therapy for human diseases, including lung fibrosis, with numerous studies on the identification, characterization, proliferation and differentiation of stem cells. A large body of both basic and pre-clinical research on stem cells has been recently translated to patient care worldwide. Herein, we review recent advances in our understanding of the pathophysiology of IPF, and types of cells used in IPF cell-based therapies, including alveolar and mixed lung epithelial cells, different stem cell types (MSCs, ADSCs, IPSCs…etc.), endogenous lung tissue-specific stem cells, and circulating endothelial progenitors (EPCs). We also discuss recent studies on the applications of these cells in IPF therapy and their delivery routes, effective doses for cell therapy, and timing of delivery. Finally, we discuss attractive recent and current clinical trials conducted on cell-based therapy for IPF.</p>","PeriodicalId":21938,"journal":{"name":"Stem cell investigation","volume":"6 1","pages":"22"},"PeriodicalIF":0.0,"publicationDate":"2019-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44768440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0