Pest Management Science最新文献

{"title":"Mechanisms mediating false codling moth, Thaumatotibia leucotreta (lepidoptera: Tortricidae), mating disruption using point‐source pheromone dispensers","authors":"Vernon Murray Steyn, Daleen Stenekamp, Antoinette Paula Malan, Pia Addison","doi":"10.1002/ps.8886","DOIUrl":"https://doi.org/10.1002/ps.8886","url":null,"abstract":"BACKGROUNDFalse codling moth (FCM) is a polyphagous Tortricid moth that is a priority agricultural pest. Mating disruption shows potential as an environmentally friendly and sustainable control option against FCM in stone fruit, table grapes and citrus. How the technique alters the mate‐finding ability of FCM and what factors are important to ensure the control success is uncertain. Recent work indicated that Lepidoptera are disrupted either competitively or non‐competitively. No studies have yet been conducted on FCM to determine its disruptive mechanism. However, studies conducted on closely related species have proposed and critically evaluated a framework, along with a dichotomous key, for distinguishing between the two mechanisms.RESULTSA protocol for identifying the underlying disruption mechanism in other species is provided. Here the dosage‐response profile, the optimal dosage and optimal density were calculated from multiple mark‐release‐recapture (MRR) experiments, using sterile FCM. Stone fruit and table grape blocks were treated with increasing levels of pheromone dispenser densities, which allowed the level of disruption experienced (FCM caught in treated/untreated blocks) to be calculated. While mating disruption proved highly effective in stone fruit and table grapes against FCM, the FCM followed a hybrid disruption profile and were disrupted competitively at low dosages and non‐competitively at higher dosages.CONCLUSIONThe shift to non‐competitive disruption, which resulted in 99% disruption, was achieved at a dosage of 800 dispensers/ha uniformly distributed and remained effective with as few as 36 pheromone release sites per ha. Mating disruption is, therefore, highly recommended to form part of the current integrated pest management program for FCM. © 2025 The Author(s). <jats:italic>Pest Management Science</jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"8 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Niemann–Pick C2 proteins play crucial role in perception of plant volatiles in Tetranychus cinnabarinus","authors":"Yuting Wang, Chenglong Wang, Wenchengxin Liu, Qianqian Huang, Wei Xiao","doi":"10.1002/ps.8839","DOIUrl":"https://doi.org/10.1002/ps.8839","url":null,"abstract":"BACKGROUNDHerbivorous mites perceive odorants through olfactory mechanisms, though the molecular basis of olfaction in these species remains poorly understood. Recent studies have identified Niemann–Pick C2 (NPC2) proteins as odorant carriers in the peripheral olfactory systems of insects. Multiple <jats:italic>NPC2</jats:italic> genes have been discovered in spider mite genomes, yet their specific roles in olfactory function have not been fully explored.RESULTSBehavioral assays showed that 8 of the 12 tested plant volatiles elicited either repellent or attractive responses in <jats:italic>Tetranychus cinnabarinus</jats:italic>. Two <jats:italic>NPC2</jats:italic> genes were identified as responsive to odorant stimulation. Recombinant NPC2 proteins were produced, and microscale thermophoresis (MST) assays revealed specific binding: TcinNPC2‐2 bound to geranylacetone [dissociation constant (Kd) = 3.32 μ<jats:sc>m</jats:sc>], and TcinNPC2‐3 bound to farnesol (Kd = 9.55 μ<jats:sc>m</jats:sc>). Knockdown of <jats:italic>NPC2</jats:italic> genes via RNA interference abolished mite responses to these odorants in behavioral assays.CONCLUSIONSMite olfactory responses to a number of odorants were widely documented. NPC2‐2 and NPC2‐3 proteins play critical roles in the olfactory detection of geranylacetone and farnesol, respectively. This study enhances our understanding of the peripheral olfactory processes in herbivorous mites, offering insights for the development of behavior‐targeting agents for mite control. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"26 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143926670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenic diversity and fungicide sensitivity of soybean root rot oomycetes in Heilongjiang Province, China","authors":"Zhanyun Liu, Yige Yang, Xueyang Wang, Ziyuan Chen, Xueyi Ruan, Zhengjie Chang, Zhongqiao Huang, Wenna Gao, Can Zhang, Xili Liu","doi":"10.1002/ps.8881","DOIUrl":"https://doi.org/10.1002/ps.8881","url":null,"abstract":"BACKGROUNDSoybean root rot is a global threat to soybean yield and quality caused by several pathogens, which vary in fungicide sensitivity. Management is challenging due to fungicide resistance and limited synergistic strategies. To understand the pathogenic diversity, the pathogens causing soybean root rot in Heilongjiang Province were identified. The inhibitory activity of different fungicides and synergistic fungicide combinations was further investigated to further develop integrated management strategies for soybean root rot.RESULTSThis study analyzed soybean root rot samples from 14 locations in Heilongjiang Province, China, identifying four oomycete species in three genera: <jats:italic>Phytophthora sojae</jats:italic>, <jats:italic>Pythium periplocum</jats:italic>, <jats:italic>Pythium acanthicum</jats:italic>, and <jats:italic>Phytopythium chamaehyphon</jats:italic>, the latter reported for the first time in China as a root rot pathogen. Sensitivity tests on six fungicides showed fluopicolide, azoxystrobin, and dimethomorph were effective against <jats:italic>P. sojae</jats:italic> but not <jats:italic>Pythium periplocum</jats:italic>, <jats:italic>Py. acanthicum</jats:italic> or <jats:italic>Ph. chamaehyphon</jats:italic>. Oxathiapiprolin is effective against <jats:italic>P. sojae</jats:italic> and <jats:italic>Ph. chamaehyphon</jats:italic>. Molecular docking revealed pathogen‐specific binding affinities, confirming their selective efficacy. Metalaxyl‐M and zoxamide displayed broad‐spectrum activity against all three genera. A synergistic 5:1 combination of these fungicides significantly reduced median effective concentration (EC<jats:sub>50</jats:sub>) values across pathogens, offering an effective integrated management approach.CONCLUSIONThe results enhance our understanding of soybean root rot pathogens, fungicide sensitivities, and optimal combinations. The identification of <jats:italic>Ph. chamaehyphon</jats:italic> and the discovery of an effective metalaxyl‐M and zoxamide combination provide foundations for sustainable control strategies to mitigate this devastating disease. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"38 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan nanoparticle‐mediated delivery of dsRNA for enhancing RNAi efficiency in Locusta migratoria","authors":"Yangyang Liu, Jianqin Zhang, Shuai Li, Lin Chai, Babar Hussain Chang, Marian Malak, Abeer El Wakil, Bernard Moussian, Zhangwu Zhao, Zhanghua Zeng, Kun Yan Zhu, Jianzhen Zhang","doi":"10.1002/ps.8880","DOIUrl":"https://doi.org/10.1002/ps.8880","url":null,"abstract":"BACKGROUNDRNA interference (RNAi) targeting the key genes involved in insect growth and development has been demonstrated as pollution‐free green pest management strategies. Double‐stranded RNA (dsRNA)‐based biopesticides offer species‐specific pest control and degrade rapidly in the environment, making them a safer alternative to conventional chemical pesticides. However, efficient delivery of dsRNA is a significant challenge in the application of RNAi technology for pest management.RESULTSChitosan, a natural biopolymer, was selected to generate nanoparticles with dsRNA of <jats:italic>LmCht10</jats:italic>, which is responsible for chitin degradation, for delivery of ds<jats:italic>LmCht10</jats:italic> in <jats:italic>Locusta migratoria</jats:italic>. Compared with the naked ds<jats:italic>LmCht10</jats:italic>, application of chitosan/ds<jats:italic>LmCht10</jats:italic> nanoparticles enhanced the stability of dsRNA in the locust gut fluid <jats:italic>in vitro</jats:italic>. Feeding the locusts with chitosan/ds<jats:italic>LmCht10</jats:italic> nanoparticles resulted in a 67% decrease of <jats:italic>LmCht10</jats:italic> transcripts and a 2‐fold increase in locust mortality. Injection of chitosan/ds<jats:italic>LmCht10</jats:italic> into the locust body cavity substantially improved RNAi efficiency against <jats:italic>LmCht10</jats:italic> by 96.6% associated with a 2‐fold increase in locust mortality. In addition, the absorption of chitosan/ds<jats:italic>LmCht10</jats:italic> nanoparticles by locust epidermal cells increased significantly and continuously by 7.3 to 8.3 times.CONCLUSIONThis study has demonstrated that chitosan‐based dsRNA nanomaterials can significantly improve the stability of dsRNA in the midgut fluid, enhance RNAi efficiency, and increase insect mortality. Moreover, chitosan‐based dsRNA delivery enhances silencing efficiency by increasing the uptake of dsRNA in the epidermal cells. Our results suggest that the use of chitosan nanomaterial for dsRNA delivery is a feasible strategy for advancing the application of RNAi technology in pest management. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"15 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143920176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling parasitoid development: climate change impacts on Telenomus remus (Nixon) and Trichogramma foersteri (Takahashi) in southern Brazil","authors":"Fábio Sampaio, Cesar A. Marchioro, Luís A. Foerster","doi":"10.1002/ps.8888","DOIUrl":"https://doi.org/10.1002/ps.8888","url":null,"abstract":"The egg parasitoids <i>Telenomus remus</i> (Nixon) and <i>Trichogramma foersteri</i> (Takahashi) were recently collected in southern Brazil, expanding their potential use in biological control. Understanding how these species respond to temperature is essential to the effective implementation of biological control programs, especially in the context of global warming. In this study, phenological models were employed to assess the effects of temperature and climate change on their development.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"30 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutating the cysteine residues within the WCCH motif of tobacco curly shoot virus (TbCSV) V2 protein attenuates viral infection and confers cross‐protection against infection by severe strain","authors":"Zeyi Xiao, Jinmin Deng, Jiayao Liu, Yuan Yuan, Weiyin Tian, Shengyu Jiang, Qiao Li, Yang Yang, Gentu Wu, Ling Qing, Mingjun Li","doi":"10.1002/ps.8869","DOIUrl":"https://doi.org/10.1002/ps.8869","url":null,"abstract":"BACKGROUNDTobacco curly shoot virus (TbCSV, <jats:italic>Begomovirus</jats:italic>, <jats:italic>Geminiviridae</jats:italic>) is a viral agent that poses a serious threat to economically important crops like tomato and tobacco in Southwest China. The geminiviral V2 protein has been demonstrated to play multifunctional roles essential for viral infection and pathogenicity. This study comprehensively investigated the biological importance of five cysteines within the conserved ‘WCCH’ motif of the TbCSV V2 protein, and evaluated the effects of cysteine mutations on viral pathogenicity and the efficacy for cross‐protection against wild‐type severe TbCSV train.RESULTSSite‐directed mutagenesis revealed that among the five cysteines in the V2 protein, C84, C86 and C89 residues are critical for its RNA silencing suppression activity, with C84 being the only residue whose mutation impaired its ability to induce tissue necrosis when expressed by potato virus X (PVX). Mutations at C84, C86 or C89 attenuated viral pathogenicity, and the mild symptoms were accompanied by reduced viral accumulations in <jats:italic>Nicotiana benthamiana</jats:italic> plants. Notably, pre‐inoculation with the attenuated TbCSV mutant C84A in <jats:italic>N. benthamiana</jats:italic> and tomato plants conferred effective cross‐protection against subsequent challenge infection by wild‐type TbCSV.CONCLUSIONThe cysteine residues within the ‘WCCH’ motif of TbCSV V2 protein play distinct roles in its functions and viral pathogenicity. Importantly, the attenuated C84A mutant generated in this study not only offers insights into the pathogenic mechanisms of TbCSV but also holds promise for application in crop protection through cross‐protection strategy in the field. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"10 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143920177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing heterospecific sterile insect technique for pest control: insights from the spotted wing fly Drosophila suzukii","authors":"Flavia Cerasti, Massimo Cristofaro, Valentina Mastrantonio, Jessica Scifo, Adriano Verna, Daniele Canestrelli, Daniele Porretta","doi":"10.1002/ps.8875","DOIUrl":"https://doi.org/10.1002/ps.8875","url":null,"abstract":"BACKGROUNDReproductive interference (i.e. sexual interaction between males of one species and females of another species that reduce the fitness of one or both the interacting individuals) is an important species interaction significantly affecting population dynamics and persistence. However, its exploitation in pest control remains overlooked. Here, we investigated the possible integration of reproductive interference into the sterile insect technique (SIT) to develop a heterospecific SIT (h‐SIT). Under this approach, contrary to the classic SIT, sterile heterospecific males from closely related, nonpest species are released to compete with the pest population for mates. To this end, we focused on the invasive pest species <jats:italic>Drosophila suzukii</jats:italic> and used <jats:italic>D. melanogaster</jats:italic> as the control species. First, we investigated the effect of irradiation on <jats:italic>D. melanogaster</jats:italic> sterility and longevity. Then, we tested the mating performance of irradiated males and their ability to reduce the <jats:italic>D. suzukii</jats:italic> fitness.RESULTSWe found by microcosm experiments that: (i) irradiation induced high levels of <jats:italic>D. melanogaster</jats:italic> male sterility without reducing longevity; (ii) irradiated <jats:italic>D. melanogaster</jats:italic> males court <jats:italic>D. suzukii</jats:italic> females as much as <jats:italic>D. suzukii</jats:italic> males do, and they couple, mate with and inseminate heterospecific females; (iii) irradiated <jats:italic>D. melanogaster</jats:italic> males significantly reduce the offspring of <jats:italic>D. suzukii</jats:italic> females under two different species ratios.CONCLUSIONOur results provide the first foundations for the development of a h‐SIT against <jats:italic>D. suzukii</jats:italic>, an approach which can be tested against other groups of pest species. © 2025 The Author(s). <jats:italic>Pest Management Science</jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"35 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143920275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced accumulation of indole glucosinolate and resistance to insect and pathogen in flowering Chinese cabbage by overexpression of Arabidopsis CYP79B2 and CYP83B1.","authors":"Yue Hou,Xinyi Zhou,Zichen Wu,Shufang Jiang,Meifang Wu,Mujun Huang,Zhiwei Shen,Jianguo Wu,Huihui Fang,Seung-Beom Hong,Yanting Zhao,Zhujun Zhu,Yunshuai Huang,Yunxiang Zang","doi":"10.1002/ps.8871","DOIUrl":"https://doi.org/10.1002/ps.8871","url":null,"abstract":"BACKGROUNDIndole glucosinolates (IGs) are a group of phytochemicals that are derived from tryptophan and mainly found in Brassicaceae. The breakdown products play an important role in insect and disease resistance. To metabolically engineer the IG pathway flux in flowering Chinese cabbage (Brassica rapa L. ssp. chinensis var. utilis Tsen et Lee), binary constructs comprising two Arabidopsis cytochrome P450 complementary DNAs (cDNAs), AtCYP79B2 and AtCYP83B1, under the control of a cauliflower mosaic virus 35S promoter were tandemly introduced into flowering Chinese cabbage via Agrobacterium-mediated genetic transformation.RESULTSThe results showed that overexpression of AtCYP79B2 and AtCYP83B1 was accompanied by higher accumulation levels of IG in the transformed plants compared to the wild-type control. Glucobrassicin was the main component of IG that accumulated at approximately 2 μmol/g dry weight (DW) indole-3-acetic acid. 4-Hydroxy glucobrassicin and glucobrassicin were the most affected components in transgenic plants, exhibiting an approximately 5- and 4-fold, respectively, when compared to wild-type control. In both trials of the Sclerotinia sclerotiorum infection and Spodoptera exigua feeding, the transformants significantly inhibited the growth of S. sclerotiorum and S. exigua. Transcriptomic analysis indicated that overexpression of AtCYP79B2/83B1 cDNAs led to the up-regulation of 152 genes and down-regulation of 88 genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis suggested that the genes involved in plant-pathogen interaction, plant hormone signal transduction, and MAPK signaling pathways are primarily responsible for enhanced resistance to S. sclerotiorum and S. exigua.CONCLUSIONOur study illustrates a successful use of the genes encoding IG pathway enzymes to increase IG content and resistance to insect and fungal pathogens via regulated expression of a broad spectrum of the genes associated with the innate immune responses in flowering Chinese cabbage. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"15 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse abietane diterpenoids as multifunctional antimicrobial effects from Salvia castanea.","authors":"Pan-Pan Wang,Song Gao,Shu-Xia Liu,Ze-Rui Li,Xiu-Juan Zhang,Jin-Ming Gao,Chun-Huan Li","doi":"10.1002/ps.8873","DOIUrl":"https://doi.org/10.1002/ps.8873","url":null,"abstract":"BACKGROUNDPhytopathogenic fungal diseases significantly impact the quality of vegetables and fruits in agriculture. Salvia castanea, a Tibetan medicinal plant, possessed anti-inflammatory, antitumor, and antimicrobial activities. Therefore, we investigated the antimicrobial ingredients from this plant and clarified their protective and curative in plants in detail.RESULTSBio-guided isolation of Salvia castanea was conducted to obtain 23 abietane diterpenoids, including three new ones (Castanoids A-C). Most compounds exhibited significant antifungal activity against four fungi. Castanoid C (7) showed remarkable inhibition against Sclerotinia sclerotiorum, Valsa mali, and Botrytis cinerea, with median effective concentration (EC50) values of 1.30, 0.84, and 2.40 μg/mL, respectively, outperforming the commercial antifungal agent trifloxystrobin. In vivo, compound 7 demonstrated 100% protective and 82.26 ± 1.07% curative efficiency against Sclerotinia sclerotiorum on rape leaves at 200 μg/mL. At 300 μg/ mL, it effectively controlled tomato rot with 92.65 ± 2.55% protective activity. The observation by transmission electron microscopy disclosed that compound 7 inhibited the growth of fungi by destroying the cell membrane permeability and caused the leakage of cellular components. Additionally, compound 11 exerted potent inhibitory effects against Staphylococcus aureus and Pseudomonas syringae pv. actinidae (Psa) with minimum inhibitory concentration (MIC) value of 6.25 μm, surpassing gentamicin. Its mechanism against Staphylococcus aureus and Psa may also involve membrane damage.CONCLUSIONThe result highlights the significant antifungal and antibacterial potential of abietane diterpenoids from Salvia castanea, particularly Castanoid C. These findings suggest their broad applicability in agriculture, especially for controlling diseases in rape, apple, and tomato crops. This research provides a foundation for developing natural, effective antimicrobial agents, advancing the field of plant disease management. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"21 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat shock protein mediates the fumigation activity of terpinen-4-ol and limonene against Tribolium confusum.","authors":"Su Shi,Yuzhi Zhang,Rui Xing,Min Liao,Hailong Wu,Yong Huang,Quan Gao,Haiqun Cao","doi":"10.1002/ps.8879","DOIUrl":"https://doi.org/10.1002/ps.8879","url":null,"abstract":"BACKGROUNDTerpinen-4-ol and limonene are important active components used to control storage pests, comprising an effective strategy to reduce the utilization of chemical pesticides and postpone the development of resistance.RESULTSTerpinen-4-ol and limonene had significant fumigation activity against Tribolium confusum, which showed positive association with temperature. Two heat shock protein genes (HSPs) (TcfHSP16.8 and TcfHSP19.0) were selected as candidate targets according to RNA-sequencing data. Following RNA interference-mediated silencing of these two genes, T. confusum showed no notable disparity in eclosion rates between temperatures of 28 °C and 40 °C. Subsequent sensitivity assessments using 30% lethal concentrations demonstrated mortality rates of 58.41% (TcfHSP16.8) and 57.10% (TcfHSP19.0) with terpinen-4-ol, and 58.62% (TcfHSP16.8) and 55.95% (TcfHSP19.0) with limonene at 40 °C, respectively. Thus, silencing TcfHSP16.8 and TcfHSP19.0 increased the susceptibility of T. confusum to both compounds at 40 °C, with limonene showing a more pronounced effect.CONCLUSIONThese results suggested that HSPs mediate and enhance the sensitivity of T. confusum to terpinen-4-ol and limonene, which is important to improve the control to storage pests and reduce the utilization of chemical pesticides. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"65 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}