Pest Management Science最新文献

{"title":"Rational design and discovery of novel hydrazide derivatives as potent succinate dehydrogenase inhibitors inspired by natural d/l‐camphor","authors":"Peng Dai, Zihua Ma, Guangfu Yi, Yufei Li, Kaili Xie, Yafang Sun, Qing Xia, Zewen Liu, Weihua Zhang","doi":"10.1002/ps.8481","DOIUrl":"https://doi.org/10.1002/ps.8481","url":null,"abstract":"BACKGROUNDSuccinate dehydrogenase inhibitors (SDHIs) have rapidly become one of the fastest‐growing categories of fungicides used against plant pathogenic fungi. Recent research advancements have emphasized that structural modifications of SDHIs using naturally sourced scaffolds represent an innovative strategy for developing new, highly effective, broad‐spectrum fungicides. A novel series of <jats:sc>d/l</jats:sc>‐camphorhydrazide derivatives potentially targeting fungal succinate dehydrogenase (SDH) were designed, synthesized and evaluated for their antifungal effects against <jats:italic>Rhizoctonia solani</jats:italic>, <jats:italic>Fusarium graminearum</jats:italic>, <jats:italic>Valsa mali</jats:italic> and <jats:italic>Botrytis cinerea</jats:italic>.RESULTSAmongst them, compounds A1‐7 (<jats:sc>d</jats:sc>‐camphor) and A2‐7 (<jats:sc>l</jats:sc>‐camphor) displayed excellent <jats:italic>in vitro</jats:italic> activity against <jats:italic>R. solani</jats:italic> with median effective concentration (EC<jats:sub>50</jats:sub>) values of 0.38 and 0.48 μg mL<jats:sup>−1</jats:sup>, which were obviously superior to that of boscalid (0.87 μg mL<jats:sup>−1</jats:sup>). A2‐5 (<jats:sc>l</jats:sc>‐camphor, EC<jats:sub>50</jats:sub> = 3.27 μg mL<jats:sup>−1</jats:sup>) exhibited good activity against <jats:italic>V. mali</jats:italic>. A2‐7 (2.13 μg mL<jats:sup>−</jats:sup>1), A2‐21 (5.2 μg mL<jats:sup>−1</jats:sup>) and A1‐5 (5.15 μg mL<jats:sup>−1</jats:sup>) showed good antifungal activity against <jats:italic>F. graminearum</jats:italic> with EC<jats:sub>50</jats:sub> values below that of boscalid (5.85 μg mL<jats:sup>−1</jats:sup>). Preliminary mechanistic studies, using scanning and transmission electron microscopy, indicated that compound A1‐7 induced disordered entanglement of hyphae, shrinkage of hyphal surfaces, and vacuole swelling and rupture, which disrupted normal hyphal growth. Additionally, compound A1‐7 induced the production and accumulation of reactive oxygen species, disrupted mitochondrial membrane potential, and effectively inhibited the germination and formation of sclerotia in <jats:italic>R. solani</jats:italic>. Moreover, the molecular docking results and SDH enzyme assays yielded promising outcomes.CONCLUSIONIn this study, the designed and optimized compounds A1‐7 and A2‐7 emerged as promising candidates for SDH‐targeting fungicides, demonstrating strong antifungal activity. These compounds hold potential as new antifungal agents for further research. © 2024 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"1 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized emamectin benzoate trunk injection: addressing temperature limitations for pine wilt disease control","authors":"Min Li, Mengyao Wang, Ting Yang, Ming Xu, Yue Li, Ying Pei, Jingen Tang, Zhe Zheng, Zhaonan Sun, Guanyi Cheng, Xiaojuan Li, Huan Li, Lichao Wang, Fengmao Chen","doi":"10.1002/ps.8493","DOIUrl":"https://doi.org/10.1002/ps.8493","url":null,"abstract":"BACKGROUNDPine wilt disease (PWD), caused by the pinewood nematode <jats:italic>Bursaphelenchus xylophilus</jats:italic>, poses a significant threat to global forestry, resulting in extensive economic and ecological damage. Traditional trunk injection agents (TIAs) are limited by environmental factors, such as high winter temperatures and vigorous resin flow, particularly in southern China. This study aimed to develop an ordinary temperature trunk injection agent (OTTIA) for year‐round application and to evaluate its performance under high‐temperature conditions in comparison to commercial TIAs.RESULTSExtensive laboratory and field tests identified <jats:italic>N</jats:italic>,<jats:italic>N</jats:italic>‐Dimethylformamide (DMF) and benzyl acetate as optimal solvents and Tween‐40 as an effective emulsifier for the OTTIA formulation. The new agent was fully absorbed by <jats:italic>Pinus massoniana</jats:italic> within 3 h at temperatures exceeding 30 °C, outperforming existing commercial agents. Treated trees maintained comparable emamectin benzoate (EB) levels but achieved lower LC<jats:sub>90</jats:sub> lethal concentration values (27.65 mg L<jats:sup>−1</jats:sup>), indicating greater efficacy. The OTTIA provided protection against PWD for ≤360 days postinjection.CONCLUSIONThe development of OTTIA marks a significant advancement in PWD management, offering an effective solution for high‐temperature regions and potentially transforming year‐round treatment strategies. The results highlight the critical role of optimizing solvent and emulsifier combinations to enhance the efficacy and application of trunk injection agents, contributing to sustainable forest management and improved protection against PWD. © 2024 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"5 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance risk assessment of Rhizoctonia solani to four fungicides","authors":"Minggui Ou, Ke Hu, Min Li, Shijiang Liu, Xinchun Zhang, Xuemei Lu, Xingyu Zhan, Xun Liao, Ming Li, Rongyu Li","doi":"10.1002/ps.8490","DOIUrl":"https://doi.org/10.1002/ps.8490","url":null,"abstract":"BACKGROUNDHexaconazole, thifluzamide, difenoconazole and azoxystrobin are widely used fungicides for the control of <jats:italic>Rhizoctonia solani</jats:italic> in China. However, few studies have assessed the sensitivity and resistance risk of <jats:italic>R. solani</jats:italic> to these four fungicides.RESULTSThe sensitivities of 126 <jats:italic>R. solani</jats:italic> isolates to hexaconazole, thifluzamide, difenoconazole and azoxystrobin were determined, with average half maximal effective concentration (EC<jats:sub>50</jats:sub>) values of 0.0386, 0.0659, 0.663 and 1.508 μg mL<jats:sup>−1</jats:sup>, respectively. Field resistance monitoring of the four fungicides showed that the three isolates had moderate resistance to difenoconazole. Resistant mutants to the four fungicides were obtained by fungicide adaptation, and resistance could be stably inherited by most mutants. Compared with those of the parent isolates, the biological characteristics of hexaconazole‐resistant mutants exhibited enhanced or similar compound fitness index (CFI), whereas most of the other mutants displayed reduced or comparable CFI. There was evidence of positive cross‐resistance between hexaconazole and difenoconazole. In the presence of fungicides, the expression of the <jats:italic>CYP51</jats:italic> genes in hexaconazole‐ and difenoconazole‐resistant mutants significantly increased, the expression of <jats:italic>SDH</jats:italic> genes in thifluzamide‐resistant mutants significantly decreased, and the expression of the <jats:italic>Cyt b</jats:italic> gene in azoxystrobin‐resistant mutants did not significantly change.CONCLUSIONBased on these data, we speculated that <jats:italic>R. solani</jats:italic> had a low‐to‐medium resistance risk to four fungicides. The change of target gene expression may be one of the reasons for fungicide resistance in <jats:italic>R. solani</jats:italic>. This study provides a theoretical basis for monitoring resistance emergence and developing resistance management strategies to control <jats:italic>R. solani</jats:italic>. © 2024 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"20 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FG‐UNet: fine‐grained feature‐guided UNet for segmentation of weeds and crops in UAV images","authors":"Jianwu Lin, Xin Zhang, Yongbin Qin, Shengxian Yang, Xingtian Wen, Tomislav Cernava, Xiaoyulong Chen","doi":"10.1002/ps.8489","DOIUrl":"https://doi.org/10.1002/ps.8489","url":null,"abstract":"BACKGROUNDSemantic segmentation of weed and crop images is a key component and prerequisite for automated weed management. For weeds in unmanned aerial vehicle (UAV) images, which are usually characterized by small size and easily confused with crops at early growth stages, existing semantic segmentation models have difficulties to extract sufficiently fine features. This leads to their limited performance in weed and crop segmentation of UAV images.RESULTSWe proposed a fine‐grained feature‐guided UNet, named FG‐UNet, for weed and crop segmentation in UAV images. Specifically, there are two branches in FG‐UNet, namely the fine‐grained feature branch and the UNet branch. In the fine‐grained feature branch, a fine feature‐aware (FFA) module was designed to mine fine features in order to enhance the model's ability to segment small objects. In the UNet branch, we used an encoder–decoder structure to realize high‐level semantic feature extraction in images. In addition, a contextual feature fusion (CFF) module was designed for the fusion of the fine features and high‐level semantic features, thus enhancing the feature discrimination capability of the model. The experimental results showed that our proposed FG‐UNet, achieved state‐of‐the‐art performance compared to other semantic segmentation models, with mean intersection over union (MIOU) and mean pixel accuracy (MPA) of 88.06% and 92.37%, respectively.CONCLUSIONThe proposed method in this study lays a solid foundation for accurate detection and intelligent management of weeds. It will have a positive impact on the development of smart agriculture. © 2024 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"67 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142448768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep learning in disease vector image identification","authors":"Shaowen Bai, Liang Shi, Kun Yang","doi":"10.1002/ps.8473","DOIUrl":"https://doi.org/10.1002/ps.8473","url":null,"abstract":"Vector-borne diseases (VBDs) represent a critical global public health concern, with approximately 80% of the world's population at risk of one or more VBD. Manual disease vector identification is time-consuming and expert-dependent, hindering disease control efforts. Deep learning (DL), widely used in image, text, and audio tasks, offers automation potential for disease vector identification. This paper explores the substantial potential of combining DL with disease vector identification. Our aim is to comprehensively summarize the current status of DL in disease vector identification, covering data collection, data preprocessing, model construction, evaluation methods, and applications in identification spanning from species classification to object detection and breeding site identification. We also discuss the challenges and possible prospects for DL in disease vector identification for further research. © 2024 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"7 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142448397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and distribution of Mdace mutations involved in propoxur resistance in the house fly (Musca domestica L.) in China","authors":"Chunmei You, Lulu Zhang, Jiajia Song, Congai Zhen, Xiangyun Ji, Lei Zhang, Xiwu Gao","doi":"10.1002/ps.8487","DOIUrl":"https://doi.org/10.1002/ps.8487","url":null,"abstract":"The house fly is a public health pest due to its ability to transmit various pathogens worldwide. The suppression of house flies is frequently controlled by organophosphate and carbamate insecticides, but recurrent selection has led to the mutated target. To understand the spread and evolution of <i>Mdace</i> mutations in house flies, we sampled flies in eight different China provinces, sequenced, and then estimated the frequencies of mutations.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"102 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142448395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-pesticide delivery system based on UiO-66 with pH sensitivity for precise control of Spodoptera frugiperda.","authors":"Chaoqun Hou,Nuo Wei,Qianwei Liang,Yifei Tan,Xiang Li,Jianguo Feng","doi":"10.1002/ps.8483","DOIUrl":"https://doi.org/10.1002/ps.8483","url":null,"abstract":"BACKGROUNDMetal-organic frameworks have the advantages of easy synthesis, high loading capacity and good biocompatibility, making them essential materials for constructing pesticide nano-delivery systems. In this study, a pH-responsive nano-controlled-release formulation Chl@UiO-66 was prepared using UiO-66 as the nano-scale carrier for adsorbing chlorantraniliprole (Chl).RESULTSThe appearance, pesticide loading, release behaviour, insecticidal activity, long-term control efficacy and safety of Chl@UiO-66 for non-target organisms were extensively evaluated. The results showed that the prepared Chl@UiO-66 was a regular octahedron with a uniform particle size of 230 nm and pesticide loading of 15.62%. The release of pesticides under alkaline conditions was superior to that under acidic and neutral conditions, which showed pH-responsive performance. Chl@UiO-66 had an excellent ability to protect pesticides from ultraviolet degradation. Compared with chlorantraniliprole suspension concentrate, Chl@UiO-66 had a better control effect against Spodoptera frugiperda and long-term control efficacy. The prepared nano-controlled-release formulation had low toxicity to zebrafish, earthworms and human BEAS-2B cells.CONCLUSIONChl@UiO-66 is a new pesticide formulation with high efficacy and low toxicity that provides a smart controlled-release solution. © 2024 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"4 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid screening for resistance to Sitobion avenae (F.) and Rhopalosiphum padi (L.) in winter wheat seedlings and selection of efficient assessment methods","authors":"Ilma A Qonaah, Amma L Simon, Duncan Warner, Rosanna M Rostron, Toby J A Bruce, Rumiana V Ray","doi":"10.1002/ps.8485","DOIUrl":"https://doi.org/10.1002/ps.8485","url":null,"abstract":"<i>Sitobion avenae</i> (F.) and <i>Rhopalosiphum padi</i> (L.) are harmful pests of wheat [<i>Triticum aestivum</i> (L.)]. No genetic resistance against the aphids has been identified in commercial wheat varieties and resistance phenotyping can be time-consuming and laborious. Here, we tested a high-throughput phenotyping method to screen 29 commercial winter wheat varieties for alate antixenosis and antibiosis. We validated this method using comprehensive behavioural analyses, including alate attraction to volatile organic compounds (VOCs) and a feeding bioassay using an electrical penetration graph (EPG), subsequently highlighting possible sources of resistance.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"3 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beauveria bassiana associated with a novel biomimetic hydrogel to control Aedes albopictus through lure and kill ovitraps","authors":"Marco Friuli, Riccardo Paolo Lia, Paola Nitti, Leonardo Lamanna, Domenico Otranto, Marco Pombi, Christian Demitri, Claudia Cafarchia","doi":"10.1002/ps.8476","DOIUrl":"https://doi.org/10.1002/ps.8476","url":null,"abstract":"Within the framework of sustainable and effective control methods for <i>Aedes albopictus</i>, two different conidial suspensions, <i>Bb</i>CS-1 and <i>Bb</i>CS-2 (respectively without and with nutrients), were used as solvents for the biopolymers water-soluble 2-hydroxyethylcellulose (HEC) and sodium alginate (SA). In this way, two different classes of hydrogels were prepared for each polymer (previously shown to attract tiger mosquito oviposition) to produce HEC-based and SA-based <i>Bb</i>/Gel systems with and without nutrients. The aim was to achieve a long-lasting and cost-effective lure-and-kill oviposition substrate useful for lethal ovitraps. <i>Beauveria bassiana</i> (<i>Bb</i>) survival and growth in the different <i>Bb</i>/Gel systems were monitored for 24 days. Following the growth assay, 24-day-old <i>Bb</i>/Gel systems were tested against <i>Ae. albopictus</i> eggs through a hatching test to evaluate their lethal effect.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"58 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allelic interactions defining Raphanus raphanistrum AHAS resistance level: strong vs weak target-site AHAS resistance alleles","authors":"Mercedes Gil, Federico Garcia, Gabriela Breccia, Ana C. Ochogavía, Graciela Nestares, Pedro E. Gundel, Heping Han, Qin Yu, Stephen B. Powles, Martin Vila-Aiub","doi":"10.1002/ps.8471","DOIUrl":"https://doi.org/10.1002/ps.8471","url":null,"abstract":"Chlorsulfuron resistance and genetic dominance was evaluated in <i>Raphanus raphanistrum</i> genotypes homozygous (122-RR, 376-RR), heterozygous (122-RS, 376-RS) and compound heterozygous (122-R/376-R) for the target-site resistance mutations Ala-122-Tyr and Asp-376-Glu in the <i>AHAS</i> (acetohydroxyacid synthase) gene.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"24 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}