Stem Cell Reports最新文献_第6页

O-GlcNAcase regulates pluripotency states of human embryonic stem cells. O-GlcNA酶调节人类胚胎干细胞的多能状态。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2024-07-09 Epub Date: 2024-06-27 DOI: 10.1016/j.stemcr.2024.05.009

Qianyu Liu, Cheng Chen, Zhiya Fan, Honghai Song, Yutong Sha, Liyang Yu, Yingjie Wang, Weijie Qin, Wen Yi

{"title":"O-GlcNAcase regulates pluripotency states of human embryonic stem cells.","authors":"Qianyu Liu, Cheng Chen, Zhiya Fan, Honghai Song, Yutong Sha, Liyang Yu, Yingjie Wang, Weijie Qin, Wen Yi","doi":"10.1016/j.stemcr.2024.05.009","DOIUrl":"10.1016/j.stemcr.2024.05.009","url":null,"abstract":"Understanding the regulation of human embryonic stem cells (hESCs) pluripotency is critical to advance the field of developmental biology and regenerative medicine. Despite the recent progress, molecular events regulating hESC pluripotency, especially the transition between naive and primed states, still remain unclear. Here we show that naive hESCs display lower levels of O-linked N-acetylglucosamine (O-GlcNAcylation) than primed hESCs. O-GlcNAcase (OGA), the key enzyme catalyzing the removal of O-GlcNAc from proteins, is highly expressed in naive hESCs and is important for naive pluripotency. Depletion of OGA accelerates naive-to-primed pluripotency transition. OGA is transcriptionally regulated by EP300 and acts as a transcription regulator of genes important for maintaining naive pluripotency. Moreover, we profile protein O-GlcNAcylation of the two pluripotency states by quantitative proteomics. Together, this study identifies OGA as an important factor of naive pluripotency in hESCs and suggests that O-GlcNAcylation has a broad effect on hESCs homeostasis.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11252487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA sequencing. 通过单细胞 RNA 测序破解分化的 hPSC 衍生角膜缘干细胞的异质性。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2024-07-09 Epub Date: 2024-06-27 DOI: 10.1016/j.stemcr.2024.06.001

Meri Vattulainen, Jos G A Smits, Julian A Arts, Dulce Lima Cunha, Tanja Ilmarinen, Heli Skottman, Huiqing Zhou

{"title":"Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA sequencing.","authors":"Meri Vattulainen, Jos G A Smits, Julian A Arts, Dulce Lima Cunha, Tanja Ilmarinen, Heli Skottman, Huiqing Zhou","doi":"10.1016/j.stemcr.2024.06.001","DOIUrl":"10.1016/j.stemcr.2024.06.001","url":null,"abstract":"A comprehensive understanding of the human pluripotent stem cell (hPSC) differentiation process stands as a prerequisite for the development of hPSC-based therapeutics. In this study, single-cell RNA sequencing (scRNA-seq) was performed to decipher the heterogeneity during differentiation of three hPSC lines toward corneal limbal stem cells (LSCs). The scRNA-seq data revealed nine clusters encompassing the entire differentiation process, among which five followed the anticipated differentiation path of LSCs. The remaining four clusters were previously undescribed cell states that were annotated as either mesodermal-like or undifferentiated subpopulations, and their prevalence was hPSC line dependent. Distinct cluster-specific marker genes identified in this study were confirmed by immunofluorescence analysis and employed to purify hPSC-derived LSCs, which effectively minimized the variation in the line-dependent differentiation efficiency. In summary, scRNA-seq offered molecular insights into the heterogeneity of hPSC-LSC differentiation, allowing a data-driven strategy for consistent and robust generation of LSCs, essential for future advancement toward clinical translation.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11252539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TGF-β modulates cell fate in human ES cell-derived foregut endoderm by inhibiting Wnt and BMP signaling. TGF-β 通过抑制 Wnt 和 BMP 信号调节人 ES 细胞衍生的前肠内胚层细胞命运。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2024-07-09 Epub Date: 2024-06-27 DOI: 10.1016/j.stemcr.2024.05.010

Nina Sofi Funa, Heidi Katharina Mjoseng, Kristian Honnens de Lichtenberg, Silvia Raineri, Deniz Esen, Anuska la Rosa Egeskov-Madsen, Roberto Quaranta, Mette Christine Jørgensen, Maria Skjøtt Hansen, Jonas van Cuyl Kuylenstierna, Kim Bak Jensen, Yi Miao, K Christopher Garcia, Philip A Seymour, Palle Serup

{"title":"TGF-β modulates cell fate in human ES cell-derived foregut endoderm by inhibiting Wnt and BMP signaling.","authors":"Nina Sofi Funa, Heidi Katharina Mjoseng, Kristian Honnens de Lichtenberg, Silvia Raineri, Deniz Esen, Anuska la Rosa Egeskov-Madsen, Roberto Quaranta, Mette Christine Jørgensen, Maria Skjøtt Hansen, Jonas van Cuyl Kuylenstierna, Kim Bak Jensen, Yi Miao, K Christopher Garcia, Philip A Seymour, Palle Serup","doi":"10.1016/j.stemcr.2024.05.010","DOIUrl":"10.1016/j.stemcr.2024.05.010","url":null,"abstract":"Genetic differences between pluripotent stem cell lines cause variable activity of extracellular signaling pathways, limiting reproducibility of directed differentiation protocols. Here we used human embryonic stem cells (hESCs) to interrogate how exogenous factors modulate endogenous signaling events during specification of foregut endoderm lineages. We find that transforming growth factor β1 (TGF-β1) activates a putative human OTX2/LHX1 gene regulatory network which promotes anterior fate by antagonizing endogenous Wnt signaling. In contrast to Porcupine inhibition, TGF-β1 effects cannot be reversed by exogenous Wnt ligands, suggesting that induction of SHISA proteins and intracellular accumulation of Fzd receptors render TGF-β1-treated cells refractory to Wnt signaling. Subsequently, TGF-β1-mediated inhibition of BMP and Wnt signaling suppresses liver fate and promotes pancreas fate. Furthermore, combined TGF-β1 treatment and Wnt inhibition during pancreatic specification reproducibly and robustly enhance INSULIN+ cell yield across hESC lines. This modification of widely used differentiation protocols will enhance pancreatic β cell yield for cell-based therapeutic applications.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11252478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular and axonal transport phenotypes due to the C9ORF72 HRE in iPSC motor and sensory neurons. iPSC 运动和感觉神经元中 C9ORF72 HRE 导致的细胞和轴突运输表型。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2024-07-09 Epub Date: 2024-06-13 DOI: 10.1016/j.stemcr.2024.05.008

Jakub Scaber, Iona Thomas-Wright, Alex J Clark, Yinyan Xu, Björn F Vahsen, Mireia Carcolé, Ruxandra Dafinca, Lucy Farrimond, Adrian M Isaacs, David L Bennett, Kevin Talbot

引用次数: 0

Recommendations on fit-for-purpose criteria to establish quality management for microphysiological systems and for monitoring their reproducibility. 关于建立微观生理系统质量管理和监测其再现性的适用标准的建议。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2024-07-02 DOI: 10.1016/j.stemcr.2024.06.007

David Pamies, Jason Ekert, Marie-Gabrielle Zurich, Olivier Frey, Sophie Werner, Monica Piergiovanni, Benjamin S Freedman, Adrian Kee Keong Teo, Hendrik Erfurth, Darwin R Reyes, Peter Loskill, Pelin Candarlioglu, Laura Suter-Dick, Shan Wang, Thomas Hartung, Sandra Coecke, Glyn N Stacey, Beren Atac Wagegg, Eva-Maria Dehne, Francesca Pistollato, Marcel Leist

引用次数: 0

Year in review: 2023 to 2024. 年份回顾：2023 至 2024 年。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2024-06-11 DOI: 10.1016/j.stemcr.2024.05.007

引用次数: 0

Stemformatics data portal enables transcriptional benchmarking of lab-derived myeloid cells. Stemformatics 数据门户网站实现了实验室衍生髓系细胞的转录基准。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2024-06-11 Epub Date: 2024-05-23 DOI: 10.1016/j.stemcr.2024.04.012

Jarny Choi, Suzanne K Butcher, Paul W Angel, Jack Bransfield, Jake Barry, Noel Faux, Bobbie Shaban, Priyanka Pillai, Aleks Michalewicz, Christine A Wells

引用次数: 0

Characterization of perivascular alveolar epithelial stem cells and their niche in lung homeostasis and cancer. 血管周围肺泡上皮干细胞及其在肺稳态和癌症中的生态位的特征。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2024-06-11 Epub Date: 2024-05-16 DOI: 10.1016/j.stemcr.2024.04.009

Qian Chen, Hiroyuki Hirai, Manwai Chan, Jilei Zhang, Minsu Cho, Scott H Randell, Preetish Kadur Lakshminarasimha Murthy, Jalees Rehman, Yuru Liu

{"title":"Characterization of perivascular alveolar epithelial stem cells and their niche in lung homeostasis and cancer.","authors":"Qian Chen, Hiroyuki Hirai, Manwai Chan, Jilei Zhang, Minsu Cho, Scott H Randell, Preetish Kadur Lakshminarasimha Murthy, Jalees Rehman, Yuru Liu","doi":"10.1016/j.stemcr.2024.04.009","DOIUrl":"10.1016/j.stemcr.2024.04.009","url":null,"abstract":"Lung alveolar structure and function are maintained by subsets of alveolar type II stem cells (AT2s), but there is a need for characterization of these subsets and their associated niches. Here, we report a CD44high subpopulation of AT2s characterized by increased expression of genes that regulate immune signaling even during steady-state homeostasis. Disruption of one of these immune regulatory transcription factor STAT1 impaired the stem cell function of AT2s. CD44high cells were preferentially located near macro- blood vessels and a supportive niche constituted by LYVE1+ endothelial cells, adventitial fibroblasts, and accumulated hyaluronan. In this microenvironment, CD44high AT2 cells were more responsive to transformation by KRAS than general AT2 cells. Moreover, after bacterial lung injury, there was a significant increase of CD44high AT2s and niche components distributed throughout the lung parenchyma. Taken together, CD44high AT2 cells and their perivascular niche regulate tissue homeostasis and tumor formation.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11390684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retinal transplant immunology and advancements. 视网膜移植免疫学及进展。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2024-06-11 Epub Date: 2024-05-09 DOI: 10.1016/j.stemcr.2024.04.007

Victor L Perez, Hazem M Mousa, Kiyoharu J Miyagishima, Amberlynn A Reed, An-Jey A Su, Thomas N Greenwell, Kia M Washington

引用次数: 0

Rigor and reproducibility in human brain organoid research: Where we are and where we need to go. 人脑类器官研究的严谨性和可重复性：我们的现状与未来。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2024-06-11 Epub Date: 2024-05-16 DOI: 10.1016/j.stemcr.2024.04.008

Soraya O Sandoval, Gerarda Cappuccio, Karina Kruth, Sivan Osenberg, Saleh M Khalil, Natasha M Méndez-Albelo, Krishnan Padmanabhan, Daifeng Wang, Mark J Niciu, Anita Bhattacharyya, Jason L Stein, André M M Sousa, Elisa A Waxman, Elizabeth D Buttermore, Dosh Whye, Carissa L Sirois, Aislinn Williams, Mirjana Maletic-Savatic, Xinyu Zhao

引用次数: 0