Stem Cell Reports最新文献_第5页

A zinc finger-dependent, PRDM13-driven mechanism regulates retinal progenitor cell fate from mouse embryonic stem cells via WNT signaling. 锌指依赖、prdm13驱动的机制通过WNT信号调节小鼠胚胎干细胞的视网膜祖细胞命运。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-22 DOI: 10.1016/j.stemcr.2025.102508

Brian W Basinski, Yuanhao Huang, Qiang Li, Charukesi D Sivakumar, Tyler J Carman, Hana M Pan, Jing Xu, D Ford Hannum, Jie Liu, Rajesh C Rao

{"title":"A zinc finger-dependent, PRDM13-driven mechanism regulates retinal progenitor cell fate from mouse embryonic stem cells via WNT signaling.","authors":"Brian W Basinski, Yuanhao Huang, Qiang Li, Charukesi D Sivakumar, Tyler J Carman, Hana M Pan, Jing Xu, D Ford Hannum, Jie Liu, Rajesh C Rao","doi":"10.1016/j.stemcr.2025.102508","DOIUrl":"10.1016/j.stemcr.2025.102508","url":null,"abstract":"The transcriptional regulation underlying eye field (retinal primordium) development requires precise control, yet the mechanisms guiding lineage-specific differentiation within the central nervous system (CNS) remain incompletely understood. Using neuroectoderm (NE) organoids derived from mouse embryonic stem cells, we investigate the role of PRDM13 in eye field specification. We demonstrate that Prdm13 expression inhibits RX+ eye field fate but permits non-eye field NE differentiation, an effect that depends on its first and second zinc-finger domains. Prdm13 activates the WNT/β-catenin signaling pathway during differentiation, leading to downregulation of key transcription factors crucial for establishing the eye field. Pharmacological inhibition of WNT signaling abolishes PRDM13-mediated suppression, restoring RX+ eye field differentiation. Our work reveals a previously undescribed PRDM13-WNT signaling axis that regulates lineage-specific neural differentiation of embryonic stem cells.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102508"},"PeriodicalIF":5.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neural stem cells of the subventricular zone: A potential stem cell pool for brain repair in Parkinson's disease. 脑室下区神经干细胞：帕金森病脑修复的潜在干细胞库

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-05 DOI: 10.1016/j.stemcr.2025.102533

Marloes Verkerke, Maarten H Werkman, Vanessa Donega

引用次数: 0

Hypoxia and loss of GCM1 expression prevent differentiation and contact inhibition in human trophoblast stem cells. 缺氧和GCM1表达缺失阻碍了人滋养细胞干细胞的分化和接触抑制。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-24 DOI: 10.1016/j.stemcr.2025.102481

Jessica K Cinkornpumin, Sin Young Kwon, Anna-Maria Prandstetter, Theresa Maxian, Jacinthe Sirois, James Goldberg, Joy Zhang, Deepak Saini, Purbasa Dasgupta, Mariyan J Jeyarajah, Stephen J Renaud, Soumen Paul, Sandra Haider, William A Pastor

{"title":"Hypoxia and loss of GCM1 expression prevent differentiation and contact inhibition in human trophoblast stem cells.","authors":"Jessica K Cinkornpumin, Sin Young Kwon, Anna-Maria Prandstetter, Theresa Maxian, Jacinthe Sirois, James Goldberg, Joy Zhang, Deepak Saini, Purbasa Dasgupta, Mariyan J Jeyarajah, Stephen J Renaud, Soumen Paul, Sandra Haider, William A Pastor","doi":"10.1016/j.stemcr.2025.102481","DOIUrl":"10.1016/j.stemcr.2025.102481","url":null,"abstract":"During the first stages of embryonic development, the placenta develops under very low oxygen tension (∼1%-2% O2), so we sought to determine the regulatory role of oxygen in human trophoblast stem cells (hTSCs). We find that low oxygen promotes hTSC self-renewal but inhibits differentiation to syncytiotrophoblast (STB) and extravillous trophoblast (EVT). The transcription factor GCM1 (glial cell missing transcription factor 1) is downregulated in low oxygen, and concordantly, there is substantial reduction of GCM1-regulated genes in hypoxic conditions. Knockout of GCM1 in hTSC likewise impaired EVT and STB formation. Treatment with a phosphatidylinositol 3-kinase (PI3K) inhibitor reported to reduce GCM1 protein levels likewise counteracts spontaneous or directed differentiation. Additionally, chromatin immunoprecipitation of GCM1 showed binding near key genes upregulated upon differentiation including the contact inhibition factor CDKN1C. Loss of GCM1 resulted in downregulation of CDKN1C and corresponding loss of contact inhibition, implicating GCM1 in regulation of this critical process.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102481"},"PeriodicalIF":5.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-scale analysis of loss of chromosome Y in human pluripotent stem cells: Implications for Turner syndrome and ribosomopathies. 人类多能干细胞Y染色体缺失的大规模分析：对特纳综合征和核糖体病的影响。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-03 DOI: 10.1016/j.stemcr.2025.102471

Roni Sarel-Gallily, Keith M Gunapala, Nissim Benvenisty

引用次数: 0

Identifying enabling strategies for effective public dialogue in human embryo research. 确定在人类胚胎研究中进行有效公众对话的有利策略。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-05-01 DOI: 10.1016/j.stemcr.2025.102498

Matilda Beckett, Sarah Franklin, Peter J Rugg-Gunn

引用次数: 0

CD37 regulates the self-renewal of leukemic stem cells via integrin-mediated signaling in acute myeloid leukemia. CD37在急性髓性白血病中通过整合素介导的信号传导调节白血病干细胞的自我更新。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-17 DOI: 10.1016/j.stemcr.2025.102476

Jinyuan Lu, Lixin Lv, Xiaoxue Tian, Zheng Li, Yuting Ma, Nannan Li, Jian Wang, Guangming Wang, Yu Zeng, Wenjun Zhang, Jun Xu, Aibin Liang

{"title":"CD37 regulates the self-renewal of leukemic stem cells via integrin-mediated signaling in acute myeloid leukemia.","authors":"Jinyuan Lu, Lixin Lv, Xiaoxue Tian, Zheng Li, Yuting Ma, Nannan Li, Jian Wang, Guangming Wang, Yu Zeng, Wenjun Zhang, Jun Xu, Aibin Liang","doi":"10.1016/j.stemcr.2025.102476","DOIUrl":"10.1016/j.stemcr.2025.102476","url":null,"abstract":"Leukemic stem cells (LSCs) are a small subset of leukemia cells that drive leukemia initiation and maintenance. Herein, we report that CD37, a member of transmembrane 4 superfamily (TM4SF), regulates the survival of acute myeloid leukemia (AML) cells as well as the self-renewal of AML LSCs. The downregulation of CD37 retarded proliferation and increased apoptosis in human AML cell lines THP-1 and OCI-AML2. Deficiency of CD37 in vivo had a minimal effect on normal hematopoiesis but significantly impeded leukemia maintenance and propagation, which led to increased apoptosis and decreased cell cycle entry in AML blasts as well as impaired colony formation and declined frequency of AML LSCs in the serial transplantation. Furthermore, CD37 interacted with integrin α4β7 and activated the phosphatidylinositol 3-kinase (PI3K)-AKT pathway mediated by integrin signaling. Our study provides novel insights for targeted therapy of AML, indicating CD37 as a safe and effective target for immunotherapy.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102476"},"PeriodicalIF":5.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Competing dynamic gene regulatory networks involved in fibroblast reprogramming to hematopoietic progenitor cells. 参与成纤维细胞重编程为造血祖细胞的竞争动态基因调控网络。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-03 DOI: 10.1016/j.stemcr.2025.102473

Samiyah Shafiq, Kiyofumi Hamashima, Laura A Guest, Ali H Al-Anbaki, Fabio M R Amaral, Daniel H Wiseman, Valerie Kouskoff, Georges Lacaud, Yuin-Han Loh, Kiran Batta

{"title":"Competing dynamic gene regulatory networks involved in fibroblast reprogramming to hematopoietic progenitor cells.","authors":"Samiyah Shafiq, Kiyofumi Hamashima, Laura A Guest, Ali H Al-Anbaki, Fabio M R Amaral, Daniel H Wiseman, Valerie Kouskoff, Georges Lacaud, Yuin-Han Loh, Kiran Batta","doi":"10.1016/j.stemcr.2025.102473","DOIUrl":"10.1016/j.stemcr.2025.102473","url":null,"abstract":"Direct reprogramming of somatic cells offers a potentially safer therapeutic approach to generate patient-specific hematopoietic cells. However, this strategy is limited by stochasticity of reprogramming. Investigating the gene regulatory networks involved during reprogramming would help generate functional cells in adequate numbers. To address this, we developed an inducible system to reprogram fibroblasts to hematopoietic progenitor cells by ectopically expressing the two transcription factors SCL and LMO2. Transcriptome and epigenome analysis at different stages of reprogramming revealed uniform silencing of fibroblast genes and upregulation of the hemogenic endothelial program. Integrated analysis suggested that the transcription factors FLI1, GATA1/2, and KLF14 are direct targets of SCL/LMO2, which subsequently induce the hematopoietic program. Single-cell RNA sequencing revealed conflicting and competing fate decisions at intermediate stages of reprogramming. Inhibiting signaling pathways associated with competing neuronal fate enhanced reprogramming efficiency. In conclusion, this study identifies early/intermediate reprogramming events and associated pathways that could be targeted to improve reprogramming efficiency.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102473"},"PeriodicalIF":5.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exercise promotes the functional integration of human stem cell-derived neural grafts in a rodent model of Parkinson's disease. 在帕金森病啮齿动物模型中，运动促进人类干细胞来源的神经移植物的功能整合。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-24 DOI: 10.1016/j.stemcr.2025.102480

Niamh Moriarty, Tyra D Fraser, Cameron P J Hunt, Georgia Eleftheriou, Jessica A Kauhausen, Lachlan H Thompson, Clare L Parish

{"title":"Exercise promotes the functional integration of human stem cell-derived neural grafts in a rodent model of Parkinson's disease.","authors":"Niamh Moriarty, Tyra D Fraser, Cameron P J Hunt, Georgia Eleftheriou, Jessica A Kauhausen, Lachlan H Thompson, Clare L Parish","doi":"10.1016/j.stemcr.2025.102480","DOIUrl":"10.1016/j.stemcr.2025.102480","url":null,"abstract":"Human pluripotent stem cell (hPSC)-derived dopamine neurons can functionally integrate and reverse motor symptoms in Parkinson's disease models, motivating current clinical trials. However, dopamine neuron proportions remain low and their plasticity inferior to fetal tissue grafts. Evidence shows exercise can enhance neuron survival and plasticity, warranting investigation for hPSC-derived neural grafts. We show voluntary exercise (wheel running) significantly increases graft plasticity, accelerating motor recovery in animals receiving ectopic, but not homotopic, placed grafts, suggestive of threshold requirements. Plasticity was accompanied by increased phosphorylated extracellular signal-regulated kinase (ERK+) cells in the graft (and host), reflective of mitogen-activated protein kinase (MAPK)-ERK signaling, a downstream target of glial cell-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF), proteins that were also elevated. Verifying improved graft integration was the increase in cFos+ postsynaptic striatal neurons. These findings have direct implications for the adoption of physical therapy-based approaches to enhance neural transplantation outcomes in future Parkinson's disease clinical trials.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102480"},"PeriodicalIF":5.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MIXL1 activation in endoderm differentiation of human induced pluripotent stem cells. MIXL1在人诱导多能干细胞内胚层分化中的激活。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-24 DOI: 10.1016/j.stemcr.2025.102482

Pierre Osteil, Sarah Withey, Nicole Santucci, Nader Aryamanesh, Ignatius Pang, Nazmus Salehin, Jane Sun, Annie Qin, Jiayi Su, Hilary Knowles, Xiucheng Bella Li, Simon Cai, Ernst Wolvetang, Patrick P L Tam

引用次数: 0

Language and labels from the lab: Definitions in the stem cell-based embryo model debate. 来自实验室的语言和标签：基于干细胞的胚胎模型辩论中的定义。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-05-13 Epub Date: 2025-04-17 DOI: 10.1016/j.stemcr.2025.102477

Hafez Ismaili M'hamdi

引用次数: 0