Stem Cell Reports最新文献_第4页

Characterization and lineage tracing of a mouse adipose depot reveal properties conserved with human supraclavicular brown adipose tissue. 小鼠脂肪库的表征和谱系追踪揭示了人类锁骨上棕色脂肪组织的保守特性。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-22 DOI: 10.1016/j.stemcr.2025.102509

Liang Li, Brian J Feldman

引用次数: 0

Stem cell-based embryo models: The 2021 ISSCR stem cell guidelines revisited. 基于干细胞的胚胎模型：2021年ISSCR干细胞指南重新审视。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 DOI: 10.1016/j.stemcr.2025.102514

Amander T Clark, Heidi Cook-Andersen, Sarah Franklin, Rosario Isasi, Debra J H Mathews, Vincent Pasque, Peter J Rugg-Gunn, Patrick P L Tam, Hongmei Wang, Jan J Zylicz, Janet Rossant

{"title":"Stem cell-based embryo models: The 2021 ISSCR stem cell guidelines revisited.","authors":"Amander T Clark, Heidi Cook-Andersen, Sarah Franklin, Rosario Isasi, Debra J H Mathews, Vincent Pasque, Peter J Rugg-Gunn, Patrick P L Tam, Hongmei Wang, Jan J Zylicz, Janet Rossant","doi":"10.1016/j.stemcr.2025.102514","DOIUrl":"10.1016/j.stemcr.2025.102514","url":null,"abstract":"Human stem cell-based embryo models (SCBEMs) are a research technology with the potential to facilitate our understanding of human embryogenesis, improve assisted reproductive technology outcomes, elucidate the causes of early pregnancy failure, and provide a clearer understanding of the developmental origins of disease. Given that human SCBEMs are designed to model specific phenotypic features and developmental processes of human embryos, they raise distinct concerns from other stem cell models, such as organoids. The International Society for Stem Cell Research (ISSCR) Guidelines for Stem Cell Research and Clinical Translation, published in 2021, made recommendations for research oversight of SCBEMs and established different categories of review based on involvement of embryonic and extraembryonic lineages. However, recent progress has enabled unexpected ways to create increasingly complex models, as well as more efficient means of doing so without including all major extraembryonic lineages. A working group was tasked by the ISSCR executive to undertake a thorough reexamination of the guidelines in the light of these advances. The three main recommendations of the working group are that all research involving organized 3-dimensional human SCBEMs (1) should be subject to appropriate review, (2) must have a clear scientific rationale, and (3) must be subject to limited timelines. The proposed modifications to the ISSCR guidelines are intended to bring more clarity to the field, help guide the deliberations of researchers, oversight committees and other relevant stakeholders, and ensure continued public confidence.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":"20 6","pages":"102514"},"PeriodicalIF":5.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling diabetic alpha cell dysfunction using stem cell-derived alpha cells. 利用干细胞衍生的α细胞模拟糖尿病α细胞功能障碍。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-08 DOI: 10.1016/j.stemcr.2025.102504

Swikriti Shrestha, Lauren T Jennings, Kyle Knofczynski, Sharath B Shivakumar, Quinn P Peterson

{"title":"Modeling diabetic alpha cell dysfunction using stem cell-derived alpha cells.","authors":"Swikriti Shrestha, Lauren T Jennings, Kyle Knofczynski, Sharath B Shivakumar, Quinn P Peterson","doi":"10.1016/j.stemcr.2025.102504","DOIUrl":"10.1016/j.stemcr.2025.102504","url":null,"abstract":"Dysfunction of pancreatic alpha cells contributes to the pathophysiology of diabetes. Features of diabetic alpha cell dysfunction include glucagon hypersecretion, defects in proglucagon processing, and altered transcriptomic profile. The lack of an in vitro human alpha cell model has prevented the investigation, and potential correction, of these dysfunctional phenotypes. Here, we show that induction of endoplasmic reticulum (ER) stress in stem cell-derived alpha (SC-α) cells induces hypersecretion of glucagon. ER stress also increases the secretion of glicentin and the expression of glucagon-like peptide-1 (GLP-1), peptides produced by alternate cleavage of proglucagon by the prohormone convertase 1 (PC1/3) enzyme. Additionally, ER stress establishes a diabetic transcriptional state in SC-α cells characterized by downregulation of MAFB, as well as glycolysis and oxidative phosphorylation pathways. We show that sunitinib, a tyrosine kinase inhibitor, protects SC-α cells against the ER stress-induced glucagon hypersecretion phenotype. Thus, SC-α cell model can advance our knowledge of islets in health and diabetes.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102504"},"PeriodicalIF":5.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered proxies and the illusion of de-extinction. 精心设计的代理和消除灭绝的幻觉。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-15 DOI: 10.1016/j.stemcr.2025.102505

Dusko Ilic

引用次数: 0

How neural stem cell therapy promotes brain repair after stroke. 神经干细胞治疗如何促进脑卒中后的修复。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-22 DOI: 10.1016/j.stemcr.2025.102507

Rebecca Z Weber, Ruslan Rust, Christian Tackenberg

引用次数: 0

Discovery of NANOG enhancers and their essential roles in self-renewal and differentiation in human embryonic stem cells. NANOG增强子的发现及其在人胚胎干细胞自我更新和分化中的重要作用。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-29 DOI: 10.1016/j.stemcr.2025.102511

Jielin Yan, Renhe Luo, Bess P Rosen, Dingyu Liu, Wilfred Wong, Christina S Leslie, Danwei Huangfu

{"title":"Discovery of NANOG enhancers and their essential roles in self-renewal and differentiation in human embryonic stem cells.","authors":"Jielin Yan, Renhe Luo, Bess P Rosen, Dingyu Liu, Wilfred Wong, Christina S Leslie, Danwei Huangfu","doi":"10.1016/j.stemcr.2025.102511","DOIUrl":"10.1016/j.stemcr.2025.102511","url":null,"abstract":"Human embryonic stem cells (hESCs) are notable for their ability to self-renew and to differentiate into all tissue types in the body. NANOG is a core regulator of hESC identity, and dynamic control of its expression is crucial to maintain the balance between self-renewal and differentiation. Transcriptional regulation depends on enhancers, but NANOG enhancers in hESCs are not well characterized. Here, we report two NANOG enhancers discovered from a CRISPR interference screen in hESCs. Deletion of a single copy of either enhancer significantly reduced NANOG expression, compromising self-renewal and increasing differentiation propensity. Interestingly, these two NANOG enhancers are involved in a tandem duplication event found in certain primates including humans but not in mice. However, the duplicated counterparts do not regulate NANOG expression. This work expands our knowledge of functional enhancers in hESCs and highlights the sensitivity of the hESC state to the dosage of core regulators and their enhancers.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102511"},"PeriodicalIF":5.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem cell fate decisions: Substates and attractors. 干细胞命运的决定：亚态和吸引子。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 DOI: 10.1016/j.stemcr.2025.102532

James E Mason, Xiaokai Nie, Daniel Coca, Peter W Andrews

引用次数: 0

Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells. 利用CRISPR激活快速评估人类多能干细胞中的基因编辑产物。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-08 DOI: 10.1016/j.stemcr.2025.102499

Youjun Wu, Aaron Zhong, Alessandro Evangelisti, Mega Sidharta, Huangfu Danwei, Lorenz Studer, Ting Zhou

{"title":"Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells.","authors":"Youjun Wu, Aaron Zhong, Alessandro Evangelisti, Mega Sidharta, Huangfu Danwei, Lorenz Studer, Ting Zhou","doi":"10.1016/j.stemcr.2025.102499","DOIUrl":"10.1016/j.stemcr.2025.102499","url":null,"abstract":"Verification of genome editing in human pluripotent stem cells (hPSCs), particularly at silent loci, is desirable but challenging, as it often requires complex and time-intensive differentiation to induce their expression. Here, we establish a rapid and effective workflow for verifying genome-edited hPSC lines targeting unexpressed genes using CRISPR-mediated transcriptional activation (CRISPRa). We systematically compared the efficiency of various CRISPRa systems and identified the synergistic activation mediator (SAM) system as the most potent for activating silent genes in hPSCs. Furthermore, combining SAM with TET1, a demethylation module, enhanced the activation of methylated genes. By inducing targeted gene activation in undifferentiated hPSCs using CRISPRa, we successfully verified single- and dual-reporter lines, functionally tested degradation tag (dTAG) knockins, and validated silent gene knockouts within 48 h. This approach bypasses the need to induce target gene expression through differentiation, providing a rapid and effective assay for verifying silent gene editing at the hPSC stage.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102499"},"PeriodicalIF":5.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144038265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell competition eliminates aneuploid human pluripotent stem cells. 细胞竞争消除了非整倍体人类多能干细胞。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-22 DOI: 10.1016/j.stemcr.2025.102506

Amanda Ya, Chenhui Deng, Kristina M Godek

{"title":"Cell competition eliminates aneuploid human pluripotent stem cells.","authors":"Amanda Ya, Chenhui Deng, Kristina M Godek","doi":"10.1016/j.stemcr.2025.102506","DOIUrl":"10.1016/j.stemcr.2025.102506","url":null,"abstract":"Human pluripotent stem cells (hPSCs) maintain diploid populations for generations despite frequent mitotic errors that cause aneuploidy or chromosome imbalances. Consequently, aneuploid hPSC propagation must be prevented to sustain genome stability, but how this is achieved is unknown. Surprisingly, we find that, unlike somatic cells, uniformly aneuploid hPSC populations with heterogeneous abnormal karyotypes proliferate. Instead, in mosaic populations, cell-non-autonomous competition between neighboring diploid and aneuploid hPSCs eliminates less fit aneuploid cells, regardless of specific chromosome imbalances. Aneuploid hPSCs with lower MYC or higher p53 levels relative to diploid neighbors are outcompeted but conversely gain an advantage when MYC and p53 relative abundance switches. Thus, MYC- and p53-driven cell competition preserves hPSC genome integrity despite their low mitotic fidelity and intrinsic capacity to proliferate with an aneuploid genome. These findings have important implications for using hPSCs in regenerative medicine and for how diploid human embryos form during development despite the prevalence of aneuploidy.","PeriodicalId":21885,"journal":{"name":"Stem Cell Reports","volume":" ","pages":"102506"},"PeriodicalIF":5.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An analysis of stem cell training programs for physicians in the US-A mirage of credibility. 对美国医生干细胞培训项目的分析——可信度的海市蜃楼。

IF 5.9 2区医学

Stem Cell Reports Pub Date : 2025-06-10 Epub Date: 2025-05-29 DOI: 10.1016/j.stemcr.2025.102510

Luqman Ellythy, Mohamed Addani, Zubin Master

引用次数: 0