Somatic Cell and Molecular Genetics最新文献_第6页

Influence of DNA delivery method on gene targeting frequencies in human cells. DNA传递方式对人细胞基因靶向频率的影响。

Somatic Cell and Molecular Genetics Pub Date : 1999-01-01 DOI: 10.1023/b:scam.0000007137.28557.73

R J Yáñez, A C Porter

引用次数: 35

Inversion and transposition of Tc1 transposon of C. elegans in mammalian cells. 秀丽隐杆线虫Tc1转座子在哺乳动物细胞中的倒置和转位。

Somatic Cell and Molecular Genetics Pub Date : 1998-11-01 DOI: 10.1023/a:1024494508878

Z H Li, D P Liu, J Wang, Z C Guo, W X Yin, C C Liang

引用次数: 14

The Na+-dependent glutamate and aspartate transporter supports glutathione maintenance and survival of CHO-K1 cells. Na+依赖性谷氨酸和天冬氨酸转运体支持谷胱甘肽维持和CHO-K1细胞的存活。

Somatic Cell and Molecular Genetics Pub Date : 1998-11-01 DOI: 10.1023/a:1024438423899

R P Igo, J F Ash

引用次数: 12

Chromosomal localization of two human genes involved in phosphate homeostasis: the type IIb sodium-phosphate cotransporter and stanniocalcin-2. 参与磷酸盐稳态的两个人类基因的染色体定位:IIb型磷酸钠共转运体和斯坦钙素-2。

Somatic Cell and Molecular Genetics Pub Date : 1998-11-01 DOI: 10.1023/a:1024442524808

K E White, J Biber, H Murer, M J Econs

{"title":"Chromosomal localization of two human genes involved in phosphate homeostasis: the type IIb sodium-phosphate cotransporter and stanniocalcin-2.","authors":"K E White, J Biber, H Murer, M J Econs","doi":"10.1023/a:1024442524808","DOIUrl":"https://doi.org/10.1023/a:1024442524808","url":null,"abstract":"Extracellular phosphate concentrations are maintained by coordinated regulation of specific homeostatic mechanisms. A novel gene, the type IIb sodium-phosphate cotransporter (Npt2b), was recently cloned and is expressed within intestinal tissues, indicating that the transporter may be an important regulator of phosphate reabsorption. Another gene, human stanniocalcin-2 (STC2), was previously shown to decrease phosphate uptake into kidney cells in vitro. Because of the important role that STC2 may play in phosphate homeostasis, we considered the peptide hormone a candidate for the phosphate wasting disease autosomal dominant hypophosphatemic rickets (ADHR), previously localized to chromosome 12p13. The purpose of our study was to determine the chromosomal localization of human NPT2b and STC2. In the present work, NPT2b was localized to human chromosome 4p15-p16, and STC2 to 5q33-tel. Because STC2 did not map to 12p13, the hormone was excluded as the ADHR gene, however it should be considered a candidate for other diseases involving phosphate homeostasis.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":"24 6","pages":"357-62"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1024442524808","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21613269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

A human HP1 pseudogene maps to chromosome 11p14. 人类HP1假基因位于染色体11p14上。

Somatic Cell and Molecular Genetics Pub Date : 1998-11-01 DOI: 10.1023/a:1024490407969

A Park, L Holmer, H J Worman

引用次数: 4

Mapping and characterization of the eukaryotic early pregnancy factor/chaperonin 10 gene family. 真核早孕因子/伴侣蛋白10基因家族的定位和表征。

Somatic Cell and Molecular Genetics Pub Date : 1998-11-01 DOI: 10.1023/a:1024488422990

K M Summers, B H Fletcher, D D Macaranas, M J Somodevilla-Torres, R M Murphy, M J Osborne, N K Spurr, A I Cassady, A C Cavanagh

引用次数: 5

Sequence analysis of the 5'-flanking region of the gene encoding human HOXA-7. 人HOXA-7基因5′侧区序列分析。

Somatic Cell and Molecular Genetics Pub Date : 1998-11-01 DOI: 10.1023/a:1024446625716

M H Kim, M Cho, D Park

{"title":"Sequence analysis of the 5'-flanking region of the gene encoding human HOXA-7.","authors":"M H Kim, M Cho, D Park","doi":"10.1023/a:1024446625716","DOIUrl":"https://doi.org/10.1023/a:1024446625716","url":null,"abstract":"We have isolated and characterized the immediate 5'-flanking region (886 bp) of the gene encoding human HOXA-7. When the total sequence was compared with those of mice, 93% of the 3' 518 bp (nt 370-886) sequences were identical, in which the 245 bases just preceding the AUG initiator codon (nt 614) was as highly conserved as in the coding region (nt 614-886). Sequences further upstream (nt 1-370) by comparison were highly diverged. In the 245 bp region, 8 stop and 3 initiation (including the initiator) codons were located, and a 50-aa long presumptive polypeptide was encoded. Nucleotide sequence analysis revealed three Sp1 and one AP2 binding sites, as well as one CAAT box. However, there was no consensus sequence for a TATA box in the 5' flanking region. One RARE repeat, one krox20 and three Hox-PBC binding sites were detected. Since many of the factor recognition sites were located in the immediate 5' flanking sequences of a highly-conserved region, it might be speculated that a regulatory mechanism for Hox gene expression is conserved throughout the evolution and one possible mechanism could be at the post-translational level.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":"24 6","pages":"371-4"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1024446625716","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21612579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Analysis of mechanisms and frequency of CDKN2A/B gene loss during progression of RAS-transformed rat embryo fibroblast clones. ras转化大鼠胚胎成纤维细胞克隆过程中CDKN2A/B基因丢失的机制和频率分析。

Somatic Cell and Molecular Genetics Pub Date : 1998-11-01 DOI: 10.1023/a:1024486307061

J N Zhou, J Hashemi, K Helou, A Zhang, D Röhme, A Zetterberg, G Levan, S Linder

{"title":"Analysis of mechanisms and frequency of CDKN2A/B gene loss during progression of RAS-transformed rat embryo fibroblast clones.","authors":"J N Zhou, J Hashemi, K Helou, A Zhang, D Röhme, A Zetterberg, G Levan, S Linder","doi":"10.1023/a:1024486307061","DOIUrl":"https://doi.org/10.1023/a:1024486307061","url":null,"abstract":"Rat embryo fibroblasts (REFs) are inefficiently transformed by RAS-oncogenes. Induction of p16INK4A expression by RAS has been suggested to contribute to this resistance. Glucocorticoid hormones, (DEX), enhance REF transformation by RAS and facilitates the isolation of transformed and immortal cell lines. We show that DEX induced cell proliferation is paralleled by a decrease in Cdkn2a gene transcripts, suggesting a mechanism for hormone promotion. The mechanisms of progression into hormone independent cell lines were examined. Twenty-two of 30 clones which reached a population size of approximately 10(6) cells could be established as cell lines. All lines studied showed homozygous deletions of the Cdkn2 loci (Cdkn2a and Cdkn2b) on RNO5. LOH was found for all RNO5 genetic markers examined in 7 of 19 cell lines, suggesting non-disjunction events. In the remaining 12 cell lines, both copies of Cdkn2 appeared to be lost by deletions/rearrangements, some of which could by demonstrated by karyotype analysis. We conclude that (i) clonal expansion of RAS-transfected REF by DEX is paralleled by down-regulation of Cdkn2a expression; (ii) homozygous deletion of Cdkn2 were estimated to occur at a frequency of 2 x 10(-8)/cell/generation or higher, and (iii) deletion/rearrangements and nondisjunction appear to be the main mechanisms leading to deletion of Cdkn2.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":"24 6","pages":"327-39"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1024486307061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21613266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Chromosomal localization and genomic structure of the human arsenite-stimulated ATPase (hASNA-I). 人亚砷酸盐刺激的atp酶(hASNA-I)的染色体定位和基因组结构。

Somatic Cell and Molecular Genetics Pub Date : 1998-09-01 DOI: 10.1023/b:scam.0000007134.16744.8b

B Kurdi-Haidar, D Heath, G Lennon, S B Howell

引用次数: 3

Nucleolin promotes homologous DNA pairing in vitro. Nucleolin 在体外促进同源 DNA 配对。

Somatic Cell and Molecular Genetics Pub Date : 1998-09-01 DOI: 10.1023/b:scam.0000007129.98789.1f

B Thyagarajan, R Lundberg, M Rafferty, C Campbell

引用次数: 11