Somatic Cell and Molecular Genetics最新文献_第10页

Induction of fragility at the human RNU2 locus by cytosine arabinoside is dependent upon a transcriptionally competent U2 small nuclear RNA gene and the expression of p53. 阿拉伯糖胞嘧啶诱导人类RNU2位点的易碎性依赖于具有转录能力的U2小核RNA基因和p53的表达。

Somatic Cell and Molecular Genetics Pub Date : 1997-11-01 DOI: 10.1007/BF02673748

H L MacArthur, M L Agarwal, S Bacchetti

引用次数: 6

Fanconi anemia group A and D cell lines respond normally to inhibitors of cell cycle regulation. 范可尼贫血A组和D组细胞系对细胞周期调节抑制剂反应正常。

Somatic Cell and Molecular Genetics Pub Date : 1997-11-01 DOI: 10.1007/BF02673747

P Johnstone, C Reifsteck, S Kohler, P Worland, S Olson, R E Moses

引用次数: 12

Exon trapping and sequence-based methods of gene finding in transcript mapping of human 4p16.3. 人类4p16.3转录本定位中的外显子捕获和基于序列的基因发现方法。

Somatic Cell and Molecular Genetics Pub Date : 1997-11-01 DOI: 10.1007/BF02673751

I Pribill, G T Barnes, J Chen, D Church, A Buckler, S Baxendale, G P Bates, H Lehrach, M J Gusella, M P Duyao, C M Ambrose, J F Gusella, M E MacDonald

{"title":"Exon trapping and sequence-based methods of gene finding in transcript mapping of human 4p16.3.","authors":"I Pribill, G T Barnes, J Chen, D Church, A Buckler, S Baxendale, G P Bates, H Lehrach, M J Gusella, M P Duyao, C M Ambrose, J F Gusella, M E MacDonald","doi":"10.1007/BF02673751","DOIUrl":"https://doi.org/10.1007/BF02673751","url":null,"abstract":"We have applied exon amplification, GRAIL2 exon prediction and EST database searching to a 2 Mb segment of chromosome 4p16.3. Experimental and computational methods of identifying exons were comparable in efficiency and apparent false positive rate, but were complementary in gene identification, revealing distinct overlapping sets of expressed sequences. EST searching was most powerful when we considered only those ESTs that show evidence of splicing relative to the genomic sequence. The combination of the three gene finding methods produced a transcription map of 30 loci in this segment of 4p16.3 that includes known human genes, homologs of loci identified in rodents and several anonymous transcripts, including a putative novel DNA polymerase and a gene related to Drosophila ash1. While most of the genes in the region have been found, our data suggest that even with the entire DNA sequence available, complete saturation of the transcript map will require additional, focused experimental effort.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02673751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20580064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Demethylation of the human MDR1 5' region accompanies activation of P-glycoprotein expression in a HL60 multidrug resistant subline. 在HL60多药耐药亚系中，人mdr15 '区的去甲基化伴随着p -糖蛋白表达的激活。

Somatic Cell and Molecular Genetics Pub Date : 1997-11-01 DOI: 10.1007/BF02673749

L Desiderato, M W Davey, A A Piper

{"title":"Demethylation of the human MDR1 5' region accompanies activation of P-glycoprotein expression in a HL60 multidrug resistant subline.","authors":"L Desiderato, M W Davey, A A Piper","doi":"10.1007/BF02673749","DOIUrl":"https://doi.org/10.1007/BF02673749","url":null,"abstract":"Chemotherapy is frequently limited by the development of multidrug resistance, a major cause of which is activation of the P-glycoprotein-encoding MDR1 gene. We have previously developed a P-glycoprotein-expressing multidrug resistant subline (HL60/E8) from the non-P-glycoprotein-expressing human HL60 promyelocytic leukemia cell line. A possible cause of MDR1 silencing in HL60 cells is methylation of the promoter proximal region, thus demethylation occurring as a result of drug treatment may be responsible for MDR1 activation in the multidrug resistant subline. Using the bisulphite genomic sequencing technique we demonstrated that HL60 DNA is methylated at multiple sites within two distinct areas, one upstream and one downstream of the transcription start point. Only a single site in each area was methylated in all strands examined, with the remaining adjacent sites showing partial methylation. In contrast, DNA from the multidrug resistant HL60/E8 subline was unmethylated at essentially all sites in both areas. Thus the development of the P-glycoprotein-expressing multidrug resistant subline was associated with demethylation of the MDR1 5' region.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02673749","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20580062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Detailed analysis of a 17q21 microdissection library by sequence bioinformatics and isolation of region-specific clones. 利用序列生物信息学和区域特异性克隆分离技术对17q21微解剖文库进行详细分析。

Somatic Cell and Molecular Genetics Pub Date : 1997-09-01 DOI: 10.1007/BF02674282

K L Bentley, W L Li, F O VannBerg, J Y Choi, J Yu, F T Kao, G Ruaño

引用次数: 1

Applications of green fluorescent protein as a marker of retroviral vectors. 绿色荧光蛋白作为逆转录病毒载体标记物的应用。

Somatic Cell and Molecular Genetics Pub Date : 1997-09-01 DOI: 10.1007/BF02674280

E S Kandel, B D Chang, B Schott, A A Shtil, A V Gudkov, I B Roninson

{"title":"Applications of green fluorescent protein as a marker of retroviral vectors.","authors":"E S Kandel, B D Chang, B Schott, A A Shtil, A V Gudkov, I B Roninson","doi":"10.1007/BF02674280","DOIUrl":"https://doi.org/10.1007/BF02674280","url":null,"abstract":"The Green Fluorescent Protein (GFP) of Aequorea victoria is used as a vital fluorescent tag for the detection and isolation of genetically modified cells. Several modified variants of GFP were tested as marker genes in retroviral vectors containing different backbones and promoter combinations. Constructs allowing for reliable detection of GFP fluorescence and the expression of a cotransduced gene from a strong promoter were identified. Cells harboring such constructs are detectable by flow cytometry, fluorescence microscopy and multi-well fluorescence reading. GFP expression in transduced cells is stable both in vitro and in vivo, and long-term dynamics of GFP-positive fractions in a mixed population can be used to monitor the biological effects of a cotransduced gene. Selection of cells with the highest GFP fluorescence enriches for multiply infected cells. The use of different GFP variants allows one to monitor simultaneously two cell populations transduced with vectors carrying GFPs that differ in their fluorescence intensity or spectral properties and to identify doubly transduced cells. In addition, transcription of an inducible promoter positioned in the opposite orientation to GFP can be monitored by the inhibition of GFP fluorescence. Thus, GFP provides a useful marker for gene transfer by retroviral vectors and extends the range of applications for retroviral transduction.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02674280","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20467826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Assignment of three human markers in chromosome 21q11 to mouse chromosome 16. 人类21q11染色体上的三个标记在小鼠16号染色体上的定位。

Somatic Cell and Molecular Genetics Pub Date : 1997-09-01 DOI: 10.1007/BF02674283

J Yu, Y Shen, S Tong, F T Kao

引用次数: 1

Correction of the Bloom syndrome cellular phenotypes. Bloom综合征细胞表型的校正。

Somatic Cell and Molecular Genetics Pub Date : 1997-09-01 DOI: 10.1007/BF02674278

T Giesler, K Baker, B Zhang, L D McDaniel, R A Schultz

引用次数: 6

Cell cycle control is aberrant in Chinese hamster ovary cell mutants exhibiting apoptosis after serum deprivation. 中国仓鼠卵巢细胞突变体在血清剥夺后出现细胞凋亡，细胞周期控制异常。

Somatic Cell and Molecular Genetics Pub Date : 1997-09-01 DOI: 10.1007/BF02674279

S Tateishi, M Yamaizumi

引用次数: 2

Phenotypic correction of ataxia-telangiectasia cellular defect by exogenously introduced human or mouse subchromosomal fragments. 外源性引入人或小鼠亚染色体片段对共济失调-毛细血管扩张细胞缺陷的表型校正。

Somatic Cell and Molecular Genetics Pub Date : 1997-09-01 DOI: 10.1007/BF02674281

Y Ejima, M S Sasaki

引用次数: 2