Somatic Cell and Molecular Genetics最新文献_第5页

The Mouse Alanine:Glyoxylate Aminotransferase Gene (Agxt1): Cloning, Expression, and Mapping to Chromosome 1 小鼠丙氨酸:乙醛酸氨基转移酶基因(Agxt1):克隆、表达和定位到1号染色体

Somatic Cell and Molecular Genetics Pub Date : 1999-03-01 DOI: 10.1023/B:SCAM.0000007142.36524.58

Xiao-Miao Li, E. Salido, L. Shapiro

引用次数: 11

Mutation Inhibition by β-Estradiol after Low Doses of γ-Irradiation of Mammalian Cells 低剂量γ辐照后β-雌二醇对哺乳动物细胞突变的抑制作用

Somatic Cell and Molecular Genetics Pub Date : 1999-03-01 DOI: 10.1023/B:SCAM.0000007141.99985.AA

T. Puck, Robert Johnson, P. Webb

引用次数: 4

Sister Chromatid Exchange Formation in Mammalian Cells Is Modulated by Deoxyribonucleotide Pool Imbalance 哺乳动物细胞姊妹染色单体交换形成受脱氧核糖核苷酸池不平衡调节

Somatic Cell and Molecular Genetics Pub Date : 1999-03-01 DOI: 10.1023/B:SCAM.0000007145.65508.D0

N. Popescu

引用次数: 10

Effects of Homology Length and Donor Vector Arrangement on the Efficiency of Double-Strand Break-Mediated Recombination in Human Cells 同源长度和供体载体排列对人细胞双链断裂介导重组效率的影响

Somatic Cell and Molecular Genetics Pub Date : 1999-03-01 DOI: 10.1023/B:SCAM.0000007144.05961.2E

J. E. Phillips, M. Calos

引用次数: 2

Effects of Sequence Divergence on the Efficiency of Extrachromosomal Recombination in Mismatch Repair Proficient and Deficient Mammalian Cell Lines 序列分化对错配修复熟练和缺陷哺乳动物细胞系染色体外重组效率的影响

Somatic Cell and Molecular Genetics Pub Date : 1999-03-01 DOI: 10.1023/B:SCAM.0000007143.08228.78

J. Villemure, A. Belmaaza

引用次数: 5

Introduction of plasmid DNA and oligonucleotides into lung epithelial cells by the hemagglutinating virus of Japan (HVJ)-liposome method. 用日本血凝病毒(HVJ)脂质体法将质粒DNA和寡核苷酸导入肺上皮细胞。

Somatic Cell and Molecular Genetics Pub Date : 1999-01-01 DOI: 10.1023/b:scam.0000007140.54724.99

M Yoshida, S Hayashi, J Sakuma-Mochizuki, K Abe, T Arai, M Mori, S Goya, H Matsuoka, Y Kaneda, T Kishimoto

{"title":"Introduction of plasmid DNA and oligonucleotides into lung epithelial cells by the hemagglutinating virus of Japan (HVJ)-liposome method.","authors":"M Yoshida, S Hayashi, J Sakuma-Mochizuki, K Abe, T Arai, M Mori, S Goya, H Matsuoka, Y Kaneda, T Kishimoto","doi":"10.1023/b:scam.0000007140.54724.99","DOIUrl":"https://doi.org/10.1023/b:scam.0000007140.54724.99","url":null,"abstract":"The hemagglutinating virus of Japan (HVJ) fused with liposomes provides a unique transfection vehicle with characteristics of both virus vector and liposome. Here we investigate the efficiency and safety of the HVJ-liposome technique in delivering foreign genes and oligonucleotides into the lung of the Wistar rat. A plasmid vector containing the Escherichia coli beta-galactosidase (beta-gal) gene and the chicken beta-actin promoter was transfected via the trachea using the HVJ-liposome method. Cytochemical staining showed expression of exogenous beta-gal activity in airway epithelial cells, alveolar macrophages, and alveolar type II cells. This activity persisted at least 28 days after administration of the genes. FITC-labeled oligonucleotides also were introduced into the same types of lung cells as those expressing beta-gal. After instillation of HVJ-liposome, anti-HVJ antibodies were detected in the sera of the rats, but even after repeated administration of HVJ-liposome, no marked histopathologic change was observed while exogenous beta-gal expression was detected in pulmonary cells.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":"25 1","pages":"49-57"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/b:scam.0000007140.54724.99","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21765443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphatase inhibitors and premature chromosome condensation in human peripheral lymphocytes at different cell-cycle phases. 不同细胞周期阶段人外周血淋巴细胞的磷酸酶抑制剂与染色体过早凝聚。

Somatic Cell and Molecular Genetics Pub Date : 1999-01-01 DOI: 10.1023/b:scam.0000007135.12486.e3

R Kanda, K Eguchi-Kasai, I Hayata

引用次数: 37

Determination of the genotype of a panel of human tumor cell lines for the human homologues of yeast cell cycle checkpoint control genes: identification of cell lines carrying homoallelic missense base substitutions. 酵母细胞周期检查点控制基因人类同源物的一组人类肿瘤细胞系的基因型测定:携带同源等位基因错义碱基替换的细胞系的鉴定。

Somatic Cell and Molecular Genetics Pub Date : 1999-01-01 DOI: 10.1023/b:scam.0000007139.83021.fc

Y Ejima, L Yang

{"title":"Determination of the genotype of a panel of human tumor cell lines for the human homologues of yeast cell cycle checkpoint control genes: identification of cell lines carrying homoallelic missense base substitutions.","authors":"Y Ejima, L Yang","doi":"10.1023/b:scam.0000007139.83021.fc","DOIUrl":"https://doi.org/10.1023/b:scam.0000007139.83021.fc","url":null,"abstract":"A number of human homologues of yeast cell cycle checkpoint control genes have been identified recently. In this study, the sequence alterations in six of such novel human genes (hRAD1, hRAD9, hRAD17, hHUS1, CHK1 and CHES1) were analyzed by PCR-single-strand conformational polymorphism (PCR-SSCP) method on a panel of 25 human tumor cell lines in an attempt to search for possible in vivo cases where any of the checkpoint-related genes are altered in human systems. For hRAD9, hHUS1 or CHK1, no SSCP variant was detected in any of the cell lines tested, indicating a high stability of these genes in human cancer. Most of the SSCP variants found in the other three genes were due to single nucleotide base substitutions. Two cell lines were found to be homozygous for missense-type base substitutions, i.e., Saos-2 was homoallelic for 1637T-->G in hRAD17; and COLO320DM for 1189G-->A in CHES1, indicating a possible use of these cell lines for further study. The former nucleotide change in hRAD17, which causes a change of amino acid from arginine to lysine at codon 546, was supposed to be polymorphic. Considering that lysine, but not arginine, is the amino acid that is well conserved among fission yeast, mouse and monkey at the corresponding position, coexistence of both alleles in human may have a functional or selectional implication.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":"25 1","pages":"41-8"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/b:scam.0000007139.83021.fc","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21765442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

The human RNA helicase A (DDX9) gene maps to the prostate cancer susceptibility locus at chromosome band 1q25 and its pseudogene (DDX9P) to 13q22, respectively. 人类RNA解旋酶A (DDX9)基因定位于染色体1q25的前列腺癌易感位点，其假基因(DDX9P)定位于染色体13q22。

Somatic Cell and Molecular Genetics Pub Date : 1999-01-01 DOI: 10.1023/b:scam.0000007138.44216.3a

C G Lee, T Eki, K Okumura, M Nogami, V da C Soares, Y Murakami, F Hanaoka, J Hurwitz

引用次数: 18

A combination of genetic suppressor elements produces resistance to drugs inhibiting DNA replication. 基因抑制因子的组合对抑制DNA复制的药物产生耐药性。

Somatic Cell and Molecular Genetics Pub Date : 1999-01-01 DOI: 10.1023/b:scam.0000007136.49230.b3

V V Levenson, E Lausch, D J Kirschling, E V Broude, I A Davidovich, S Libants, V Fedosova, I B Roninson

{"title":"A combination of genetic suppressor elements produces resistance to drugs inhibiting DNA replication.","authors":"V V Levenson, E Lausch, D J Kirschling, E V Broude, I A Davidovich, S Libants, V Fedosova, I B Roninson","doi":"10.1023/b:scam.0000007136.49230.b3","DOIUrl":"https://doi.org/10.1023/b:scam.0000007136.49230.b3","url":null,"abstract":"Many anticancer drugs inhibit DNA replication. To investigate the mechanism of permanent growth inhibition after transient arrest of DNA replication, we selected genetic suppressor elements (GSEs) conferring resistance to replication inhibitor Aphidicolin. Starting from a retroviral expression library carrying normalized fragments of human cell cDNA, we isolated four GSEs which, when introduced as a combination, produced resistance to Aphidicolin, doxorubicin and hydroxyurea in HT1080 fibrosarcoma cells. The four GSEs were derived from ORFX bromodomain protein gene, WIZ zinc finger protein gene, the gene for subunit 3 of cytochrome c oxidase, and the gene corresponding to an EST with no known function. A cell line carrying all four GSEs showed a weaker induction of the senescence-like phenotype after treatment with Aphidicolin or doxorubicin; the resistance of this cell line was not associated with decreased doxorubicin accumulation. These results indicate that combined effects of GSEs derived from these four genes increase cellular resistance to replication-inhibiting drugs, possibly by inhibiting drug-induced senescence.","PeriodicalId":21884,"journal":{"name":"Somatic Cell and Molecular Genetics","volume":"25 1","pages":"9-26"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/b:scam.0000007136.49230.b3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21765529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27