Mapping and characterization of the eukaryotic early pregnancy factor/chaperonin 10 gene family.

K M Summers, B H Fletcher, D D Macaranas, M J Somodevilla-Torres, R M Murphy, M J Osborne, N K Spurr, A I Cassady, A C Cavanagh
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引用次数: 5

Abstract

Early pregnancy factor and mitochondrial chaperonin 10 have very different functions within mammals but the mature peptides have identical amino acid sequences. In order to understand the mechanisms by which identical proteins can have different functions and sites of activity, we have examined genomic DNA which could encode the protein. In most species studied, there is a large gene family of at least ten members with homology to the DNA sequence for this protein. Using a monochromosomal somatic cell hybrid panel, we have mapped the gene for human chaperonin 10 to chromosome 2. Other members of the human gene family map to several chromosomes. Chromosomes 1, 2 and 9 contain pseudogenes with Alu insertions while chromosome 16 has a pseudogene containing a short direct repeat flanking an insert. Chromosomes 1 and 16 may also carry a functional intronless copy of the EPF/Cpn10 sequence.

真核早孕因子/伴侣蛋白10基因家族的定位和表征。
早期妊娠因子和线粒体伴侣蛋白10在哺乳动物体内具有不同的功能,但其成熟肽具有相同的氨基酸序列。为了了解相同蛋白质具有不同功能和活性位点的机制,我们研究了可以编码蛋白质的基因组DNA。在大多数被研究的物种中,存在一个至少有10个成员与该蛋白的DNA序列同源的大基因家族。利用单染色体体细胞杂交面板,我们已经将人类伴侣蛋白10的基因定位到2号染色体上。人类基因家族的其他成员映射到几条染色体。染色体1、2和9包含有Alu插入的假基因,而染色体16有一个假基因在插入的两侧包含一个短的直接重复。染色体1和16也可能携带EPF/Cpn10序列的功能性无内含子拷贝。
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