人HOXA-7基因5′侧区序列分析。

M H Kim, M Cho, D Park
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引用次数: 7

摘要

我们已经分离并鉴定了编码人类HOXA-7基因的近5'-侧翼区域(886 bp)。与小鼠总序列比较,3’518 bp (nt 370 ~ 886)序列有93%相同,其中AUG启动密码子(nt 614)前245个碱基与编码区(nt 614 ~ 886)高度保守。相比之下,更上游的序列(nt 1-370)高度分化。在245 bp区域,定位到8个终止密码子和3个起始密码子(包括启动子),编码了一个50-aa长的推定多肽。核苷酸序列分析显示3个Sp1和1个AP2结合位点,以及1个CAAT盒。然而,在5'侧翼区域没有一致的TATA盒序列。检测到1个RARE重复、1个krox20和3个fox - pbc结合位点。由于许多因子识别位点位于一个高度保守区域的近5′侧序列,因此可以推测Hox基因表达的调控机制在整个进化过程中都是保守的,一种可能的机制可能在翻译后水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sequence analysis of the 5'-flanking region of the gene encoding human HOXA-7.

We have isolated and characterized the immediate 5'-flanking region (886 bp) of the gene encoding human HOXA-7. When the total sequence was compared with those of mice, 93% of the 3' 518 bp (nt 370-886) sequences were identical, in which the 245 bases just preceding the AUG initiator codon (nt 614) was as highly conserved as in the coding region (nt 614-886). Sequences further upstream (nt 1-370) by comparison were highly diverged. In the 245 bp region, 8 stop and 3 initiation (including the initiator) codons were located, and a 50-aa long presumptive polypeptide was encoded. Nucleotide sequence analysis revealed three Sp1 and one AP2 binding sites, as well as one CAAT box. However, there was no consensus sequence for a TATA box in the 5' flanking region. One RARE repeat, one krox20 and three Hox-PBC binding sites were detected. Since many of the factor recognition sites were located in the immediate 5' flanking sequences of a highly-conserved region, it might be speculated that a regulatory mechanism for Hox gene expression is conserved throughout the evolution and one possible mechanism could be at the post-translational level.

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