Somatic Cell Genetics最新文献_第5页

Isolation and characterization of interspecific heat-resistant hybrids between a temperature-sensitive chinese hamster cell asparaginyl-tRNA synthetase mutant and normal human leukocytes: assignment of human asnS gene to chromosome 18. 温度敏感的中国仓鼠细胞天冬酰胺- trna合成酶突变体与正常人白细胞种间耐热杂交种的分离与鉴定:人asnS基因在18号染色体上的分配。

Somatic Cell Genetics Pub Date : 1983-03-01 DOI: 10.1007/BF01543178

R E Cirullo, F X Arredondo-Vega, M Smith, J J Wasmuth

{"title":"Isolation and characterization of interspecific heat-resistant hybrids between a temperature-sensitive chinese hamster cell asparaginyl-tRNA synthetase mutant and normal human leukocytes: assignment of human asnS gene to chromosome 18.","authors":"R E Cirullo, F X Arredondo-Vega, M Smith, J J Wasmuth","doi":"10.1007/BF01543178","DOIUrl":"https://doi.org/10.1007/BF01543178","url":null,"abstract":"We isolated interspecific somatic cell hybrids between human peripheral leukocytes and a temperature-sensitive CHO cell line with a thermolabile asparaginyl-tRNA synthetase. The hybrids were selected at 39 degrees C so as to require the expression of the human gene complementing the deficient CHO enzyme. In vitro heat-inactivation profiles of cell-free extracts from temperature-resistant hybrid cells indicate the presence of two forms of asparaginyl-tRNA synthetase. One form is very resistant to thermal inactivation, like the normal human enzyme, while the other form is very thermolabile, like the altered enzyme from the CHO parent. Hybrids and temperature-sensitive segregants derived from them were analyzed for the expression of known human chromosomal marker enzymes. The strong correlation between the expression of the human form of asparaginyl-tRNA synthetase and the presence of human chromosome 18 in hybrids suggests that the human gene, asnS, which corrects the heat-sensitive phenotype of the CHO asparaginyl-tRNA synthetase mutant, is located on chromosome 18.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 2","pages":"215-33"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01543178","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17888088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Alanine-resistant mutants of Chinese hamster ovary cells, CHO-K1, producing increases in velocity of proline transport through the A, ASC, and P systems. 中国仓鼠卵巢细胞CHO-K1的抗丙氨酸突变体，通过A、ASC和P系统产生脯氨酸运输速度增加。

Somatic Cell Genetics Pub Date : 1983-03-01 DOI: 10.1007/BF01543177

J Moffett, S Curriden, R Ertsey, E Mendiaz, E Englesberg

{"title":"Alanine-resistant mutants of Chinese hamster ovary cells, CHO-K1, producing increases in velocity of proline transport through the A, ASC, and P systems.","authors":"J Moffett, S Curriden, R Ertsey, E Mendiaz, E Englesberg","doi":"10.1007/BF01543177","DOIUrl":"https://doi.org/10.1007/BF01543177","url":null,"abstract":"We have developed a method for the isolation of transport mutants with increases in velocity of transport through the A and ASC systems and through a newly discovered P system utilizing the amino acid antagonism between A system amino acids and proline in CHO-K1 pro- cells. Mutants alar2 and alar3, isolated in a single-step procedure, resistant to 25 mM alanine in MEM-10 plus 0.05 mM proline are pro-, stable, cross resistant to alpha-(methylamino)isobutyric acid (MeAIB) and show an approximately twofold increase in the initial velocity of proline uptake. Ethyl methane sulfonate (EMS) increases the frequency of pro- alar clones in the population by at least 50 times the spontaneous frequency. The increased velocity of proline transport by alar2 and alar3 can be attributable to the 1.5 to 3 times increase in velocity of transport of proline through systems A, ASC, and P. The Vmax for proline transport through the A system has increased two times for alar2 while the Km and Vmax for alar3 has increased by 1.4 and 2.3 times that of CHO-K1. There is a corresponding increase in Vmax of proline transport by alar2 through the P system. The P system is defined operationally as that portion of the Na+-dependent velocity that remains when the A, ASC, and glutamine-inhibitable fraction are eliminated. The system is concentrative. Proline appears to be the preferred substrate. Li+ cannot be substituted for Na+. The system is moderately dependent upon pH. It obeys Michaelis-Menten kinetics and is not derepressible by starvation. There is no evidence for an N system in CHO-K1.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 2","pages":"189-213"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01543177","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17463814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Genetic control of drug resistance: assignment of ama-1 to Chinese hamster chromosome 7, confirmation of assignment of genes coding for TK, GALK, and ACP to chromosome 7, and tentative assignment of TPI to chromosome 8. 耐药性的遗传控制:ama-1在中国仓鼠7号染色体上的定位，TK、GALK和ACP编码基因在7号染色体上的定位确认，TPI在8号染色体上的初步定位。

Somatic Cell Genetics Pub Date : 1983-03-01 DOI: 10.1007/BF01543179

M Roberts, G A Scangos, J T Hart, F H Ruddle

引用次数: 7

Origin, cellular expression, and cybrid transmission of mitochondrial CAP-R, PYR-IND, and OLI-R mutant phenotypes. 线粒体CAP-R、PYR-IND和OLI-R突变表型的起源、细胞表达和杂交传递。

Somatic Cell Genetics Pub Date : 1983-01-01 DOI: 10.1007/BF01544045

N Howell

引用次数: 38

Complementation mapping in microcell hybrids: localization of Fpgs and Ak-1 on Mus musculus chromosome 2. 小家鼠2号染色体上Fpgs和Ak-1的定位。

Somatic Cell Genetics Pub Date : 1983-01-01 DOI: 10.1007/BF01544049

R E Fournier, R G Moran

引用次数: 30

Analysis of myogenesis by somatic cell hybridization: III. Myogenic competence of hybrids derived from rat L6 myoblasts and mouse primary fibroblasts and myoblasts. 体细胞杂交法对肌发生的分析:III。大鼠L6成肌细胞与小鼠原代成纤维细胞和成肌细胞杂种的成肌能力。

Somatic Cell Genetics Pub Date : 1983-01-01 DOI: 10.1007/BF01544046

S F Konieczny, J R Coleman

{"title":"Analysis of myogenesis by somatic cell hybridization: III. Myogenic competence of hybrids derived from rat L6 myoblasts and mouse primary fibroblasts and myoblasts.","authors":"S F Konieczny, J R Coleman","doi":"10.1007/BF01544046","DOIUrl":"https://doi.org/10.1007/BF01544046","url":null,"abstract":"Hybrid cells derived from rat L6 myoblasts and mouse primary fibroblasts (M x F hybrids), as well as those derived from rat L6 myoblasts and mouse primary myoblasts (M x M hybrids), were examined for their ability to engage in myogenesis as judged by muscle fiber formation plus the expression of skeletal muscle myosin and creatine kinase (CK). Of 172 primary hybrid colonies scored, 59% were myogenic in the M x F fusion and 97% exhibited muscle fiber formation in the M x M fusion. Individual hybrid clones from each cross were isolated, expanded and analyzed for myogenic capabilities as well. All three M x M and all ten M x F isolated clones exhibited preferential elimination of mouse chromosomes. Nonetheless, all were capable of fusing spontaneously and of elaborating skeletal muscle myosin and CK. The three M x M hybrids expressed only MM-CK whereas nine out of ten M x F hybrids produced all three CK isoenzymes (MM, MB, BB). These results suggest that M X M hybrids express CK patterns reminiscent of the rat L6 parental cells while M X F hybrids apparently mimic mouse muscle fiber CK patterns. Various models are discussed which address these phenomena.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 1","pages":"25-42"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01544046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17888083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Meeting announcements 会议公告

Somatic Cell Genetics Pub Date : 1983-01-01 DOI: 10.1007/BF01543183