Somatic Cell Genetics最新文献_第6页

RNA from rat hepatoma cells can activate phenylalanine hydroxylase gene of mouse erythroleukemia cells. 来自大鼠肝癌细胞的RNA可激活小鼠红白血病细胞苯丙氨酸羟化酶基因。

Somatic Cell Genetics Pub Date : 1983-01-01 DOI: 10.1007/BF01544053

T V Gopalakrishnan, J W Littlefield

{"title":"RNA from rat hepatoma cells can activate phenylalanine hydroxylase gene of mouse erythroleukemia cells.","authors":"T V Gopalakrishnan, J W Littlefield","doi":"10.1007/BF01544053","DOIUrl":"https://doi.org/10.1007/BF01544053","url":null,"abstract":"Mouse erythroleukemia (MEL) cells do not synthesize any detectable level of phenylalanine hydroxylase and thus do not grow in Tyr- medium. Rat hepatoma cells that constitutively express phenylalanine hydroxylase were treated prior to fusion with MEL cells with biochemical inhibitors to inactivate different macromolecular components of the cells, and the fusion products were selected in Tyr- medium. Continuously growing populations of cells resembling the parental MEL cells and expressing mouse phenylalanine hydroxylase were obtained only when rat hepatoma cells treated with mitomycin or iodoacetamide, which inactivate DNA and SH proteins, respectively, were fused with MEL cells. Fusion of MEL cells with UV-treated rat hepatoma cells did not result in the activation of the mouse phenylalanine hydroxylase gene. UV treatment damages both DNA and RNA. These data suggested that RNA was involved in the regulation of phenylalanine hydroxylase gene. Additional evidence for the role of RNA in the phenylalanine hydroxylase gene regulation was obtained from RNA transfection studies. RNA only from cells which express phenylalanine hydroxylase, such as rat hepatoma cells and MEL cybrids, when introduced into MEL cells by the CaPO4 coprecipitation method, resulted in the permanent activation of the mouse phenylalanine hydroxylase gene.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 1","pages":"121-31"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01544053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17630043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Selection of membrane IgM- variants from a mIgM+ murine B lymphoma cell: problems and solutions. 从mIgM+小鼠B淋巴瘤细胞中选择膜IgM-变异:问题和解决方案。

Somatic Cell Genetics Pub Date : 1983-01-01 DOI: 10.1007/BF01544047

R Andrews-Wagner, C H Sibley

引用次数: 6

Changes in the expression of differentiated functions during long-term cultivation of rat hepatoma cells. 大鼠肝癌细胞长期培养过程中分化功能表达的变化。

Somatic Cell Genetics Pub Date : 1983-01-01 DOI: 10.1007/BF01544050

A Venetianer, Z Bösze

引用次数: 4

Human cell variants resistant to methylglyoxal-bis(guanylhydrazone) display increased sensitivity to chloramphenicol. 对甲基乙二醛-双(鸟酰腙)耐药的人类细胞变异对氯霉素的敏感性增加。

Somatic Cell Genetics Pub Date : 1983-01-01 DOI: 10.1007/BF01544051

A Wiseman, C W Porter

{"title":"Human cell variants resistant to methylglyoxal-bis(guanylhydrazone) display increased sensitivity to chloramphenicol.","authors":"A Wiseman, C W Porter","doi":"10.1007/BF01544051","DOIUrl":"https://doi.org/10.1007/BF01544051","url":null,"abstract":"Four variants of the cultured human cell line VA2 have been isolated which are resistant to the antiproliferative and antimitochondrial effects of methylglyoxal-bis(guanylhydrazone) (MGBG). Each of the four variants is two- to fivefold more sensitive to the mitochondrial protein synthesis inhibitor chloramphenicol (CAP) than wild type when grown in the absence of MGBG, and five- to tenfold more sensitive to CAP when grown in the presence of MGBG. Uptake studies demonstrate that each MGBG-resistant variant cell line is freely permeable to CAP. The in vivo rates of mitochondrial protein synthesis are significantly reduced in each of the variants whether pregrown and labeled in the presence or absence of MGBG. When cytoplasts from a cytoplasmically inherited CAP-resistant mutant are fused to an MGBG-resistant recipient cell line, cybrid clones can be isolated which are functionally resistant to low levels of CAP. With continued growth, the levels of resistance to CAP do not, however, approach the levels of resistance of the CAP-resistant donor cell line. When CAP resistance is subsequently transferred from a CAP/MGBG-resistant cybrid by enucleation and fusion to other human cell lines, then CAP-resistant cybrids can be readily selected in high levels of CAP. It is possible that the substantial decrease in mitochondrial protein synthesis observed in the variants fully accounts for their increased sensitivity to CAP, although the basis for this decreased rate of mitochondrial protein synthesis is not understood.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 1","pages":"95-110"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01544051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17888085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Phenotype of low proliferative potential is dominant in hybrids of normal human fibroblasts. 在正常人成纤维细胞的杂交中，低增殖潜能的表型占主导地位。

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543015

O M Pereira-Smith, J R Smith

引用次数: 58

Rapid complementation method for classifying excision repair-defective xeroderma pigmentosum cell strains. 快速互补法分类切除修复缺陷性着色性干皮病细胞株。

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543020

J E Cleaver

引用次数: 13

Genetic analysis of tumorigenesis: XII. Genetic control of the anchorage requirement in CHEF cells. 肿瘤发生的遗传分析:12。主厨细胞锚定需求的遗传控制。

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543013

C J Marshall, R M Kitchin, R Sager

引用次数: 11

HLA antigen expression at the single cell level on a K562 X B cell hybrid: an analysis with monoclonal antibodies using bacterial binding assays. HLA抗原在K562 X B细胞杂交株单细胞水平上的表达:单克隆抗体细菌结合分析

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543018

A Ziegler, B Uchańska-Ziegler, J Zeuthen, P Wernet

{"title":"HLA antigen expression at the single cell level on a K562 X B cell hybrid: an analysis with monoclonal antibodies using bacterial binding assays.","authors":"A Ziegler, B Uchańska-Ziegler, J Zeuthen, P Wernet","doi":"10.1007/BF01543018","DOIUrl":"https://doi.org/10.1007/BF01543018","url":null,"abstract":"The influence of different genetic environments on the expression of HLA complex-controlled antigens has been investigated using cell lines with various defects in the synthesis of these molecules and a somatic cell hybrid derived from them. A very sensitive bacterial binding assay allowing simultaneous evaluation of the morphology of a given cell and the quantity of a surface molecule has been developed for these studies. The fetal erythroid cell line K562, the Burkitt's lymphoma-derived cell line DAUDI, and their hybrid DUTKO1 have been employed. K562 and the hybrid, but not DAUDI, expressed HLA-A,B,C heavy chains as detected by the monoclonal antibody W6/32.HL, while two monoclonal antibodies (TU48 and 2BC4) against the supertypic specificities HLA-Bw4 and Bw6 showed no reactivity. The presence of human Ia-like antigens on the cell surfaces was investigated with a panel of eight monoclonal antibodies. K562 cells were completely unreactive, and DAUDI cells gave the expected positive reaction, but about 1% or less of the cells in the DUTKO1 population appeared to express these antigens as well. We discuss possible reasons for the failure to detect HLA-B antigens with monoclonal antibodies and the lack of complete \"dominance\" of the K562 genome in the hybrid cell line.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"8 6","pages":"775-89"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01543018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17251011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 81

Genetic and biochemical characterization of a human surface determinant on somatic cell hybrids: the 4F2 antigen. 体细胞杂交的人表面决定因素的遗传和生化特征:4F2抗原。

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543022

P G Peters, M E Kamarck, M E Hemler, J L Strominger, F H Ruddle

引用次数: 37

Mapping of the genes of some components of the electron transport chain (complex I) on the X chromosome of mammals. 哺乳动物X染色体上电子传递链(复合体I)某些组分的基因图谱。

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543012

C E Day, I E Scheffler

引用次数: 16