Somatic Cell Genetics最新文献_第7页

Large-scale selection and analysis of temperature-sensitive mutants for cell reproduction from BHK cells. BHK细胞中温度敏感突变体的大规模选择和细胞繁殖分析。

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543021

T Nishimoto, T Sekiguchi, R Kai, K Yamashita, T Takahashi, M Sekiguchi

{"title":"Large-scale selection and analysis of temperature-sensitive mutants for cell reproduction from BHK cells.","authors":"T Nishimoto, T Sekiguchi, R Kai, K Yamashita, T Takahashi, M Sekiguchi","doi":"10.1007/BF01543021","DOIUrl":"https://doi.org/10.1007/BF01543021","url":null,"abstract":"Temperature-sensitive (ts) mutant cells for cell reproduction were isolated from the Syrian hamster cell line BHK21/13 by multiple culturing in the presence of 5-fluoro-2'-deoxyuridine (FdU) at 37.5 degrees after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. A simple method for cell fusion was devised, which enabled us to perform complementation studies with a large number of ts mutants. By using the method we have analyzed 219 ts mutants and classified them into 18 complementation groups. Mutants that belonged to the same complementation groups tended to exhibit similar patterns of inhibition of DNA synthesis at 39.5 degrees; however, some mutants belonging to the same group showed somewhat different patterns, probably due to occurrence of different mutations in the same gene. Distribution of the ts mutants among the 18 complementation groups was uneven; more than 50% of the mutants examined were assigned to complementation group B and G. The mutations belonging to complementation group B and G were found to be linked to the X chromosome.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"8 6","pages":"811-24"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01543021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17940689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42

Modification of the cataractous phenotype by somatic cell hybridization. 体细胞杂交对白内障表型的修饰。

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543019

R D Lipman, A L Muggleton-Harris

引用次数: 8

Rescue of terminally differentiating teratocarcinoma cells by fusion to undifferentiated parental cells. 通过与未分化的亲代细胞融合来拯救终末分化的畸胎癌细胞。

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543016

J W Littlefield, J S Felix

引用次数: 17

Enhancement of polyethylene glycol-mediated cell hybridization by inducers of erythroleukemia cell differentiation. 聚乙二醇介导的红细胞白血病细胞分化诱导因子增强细胞杂交。

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543014

R J Klebe, M G Mancuso

引用次数: 13

Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions. 对含有紫外线损伤的DNA切割缺陷的CHO细胞突变体的突变和姐妹染色单体交换诱导的超敏反应。

Somatic Cell Genetics Pub Date : 1982-11-01 DOI: 10.1007/BF01543017

L H Thompson, K W Brookman, L E Dillehay, C L Mooney, A V Carrano

{"title":"Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions.","authors":"L H Thompson, K W Brookman, L E Dillehay, C L Mooney, A V Carrano","doi":"10.1007/BF01543017","DOIUrl":"https://doi.org/10.1007/BF01543017","url":null,"abstract":"Five UV-sensitive mutant strains of CHO cells representing different genetic complementation groups were analyzed for their ability to perform the incision step of nucleotide excision repair after UV exposure. The assay utilized inhibitors of DNA synthesis to accumulate the short-lived strand breaks resulting from repair incisions. After 6 J/m2, each of the mutants showed less than 10% of the incision rate of the parental AA8 cells. After 50 J/m2, the rate in AA8 was similar to that at 6 J/m2, but the rates in the mutants were significantly higher (approximately 20% of the rate of AA8). Thus by this incision assay the mutants were phenotypically indistinguishable. Each of the mutants were hypersensitive to mutation induction at both the hprt and aprt loci by a factor of 10, and in the one strain tested ouabain resistance was induced sevenfold more efficiently than in AA8 cells. Sister chromatid exchange was also induced with sevenfold increased efficiency in the two mutant strains examined. Thus, these CHO mutants resemble xeroderma pigmentosum cells in terms of their incision defects and their hypersensitivity to DNA damage by UV.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"8 6","pages":"759-73"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01543017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18179114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 129

The BrdU content of DNA is decreased during reversal of inhibition of myogenesis by deoxycytidine. 脱氧胞苷抑制肌生成的逆转过程中，DNA的BrdU含量降低。

Somatic Cell Genetics Pub Date : 1982-09-01 DOI: 10.1007/BF01542850

W E Wright

引用次数: 5

Analysis of early antigenic changes on heterokaryons between L-cells and a teratocarcinoma-derived cell line, TerC. l细胞与畸胎癌来源细胞系TerC间异核体早期抗原变化分析。

Somatic Cell Genetics Pub Date : 1982-09-01 DOI: 10.1007/BF01542853

D B Searls

引用次数: 0

Dominant allele-specific regulation of expression of H-2Kk gene products revealed by somatic cell hybridization. 体细胞杂交揭示的显性等位基因特异性调控H-2Kk基因产物的表达。

Somatic Cell Genetics Pub Date : 1982-09-01 DOI: 10.1007/BF01542855

C E Bishop, C M Lewis, H Festenstein

引用次数: 5

Characterization of dominant hamster cell mutants resistant to oxygenated sterols. 抗氧化甾醇的优势仓鼠细胞突变体的特征。

Somatic Cell Genetics Pub Date : 1982-09-01 DOI: 10.1007/BF01542851

W K Cavenee, R M Baker

引用次数: 3

Mutagen treatment of single Chinese hamster ovary cells produces colonies mosaic for glucose-6-phosphate dehydrogenase activity. 诱变剂处理单个中国仓鼠卵巢细胞产生葡萄糖-6-磷酸脱氢酶活性的集落镶嵌。

Somatic Cell Genetics Pub Date : 1982-09-01 DOI: 10.1007/BF01542857

T D Stamato, L MacKenzie, J M Pagani, R Weinstein

{"title":"Mutagen treatment of single Chinese hamster ovary cells produces colonies mosaic for glucose-6-phosphate dehydrogenase activity.","authors":"T D Stamato, L MacKenzie, J M Pagani, R Weinstein","doi":"10.1007/BF01542857","DOIUrl":"https://doi.org/10.1007/BF01542857","url":null,"abstract":"When populations of single Chinesee hamster ovary (CHO) cells were exposed to the mutagen ethyl methane sulfonate (EMS), allowed to grow into colonies, and stained for glucose-6-phosphate dehydrogenase (G6PD) activity, two types of unstained colonies were observed at a frequency of about on per thousand stained colonies. These negative-staining colonies consisted of (1) colonies uniformly deficient in staining activity (pure); and (2) colonies containing both stained and unstained sectors (mosaic) in various relative sizes and patterns. Unstained cells isolated from mosaic colonies were genetically stable and had significantly reduced or absent G6PD activity. Random cell aggregation or chromosome segregation from tetraploid cells is not a significant cause of the sectoring phenomenon. Also, mosaic colonies are not principally caused by mutation at one of two replicated G6PD genes and their subsequent segregation during division. The simplest explanation for this phenomenon is that EMS induces a mutational change in one of the two DNA strands and DNA replication then produces normal and mutant double-stranded DNAs which segregate into wild-type and G6PD-deficient cell types, producing a mosaic colony.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"8 5","pages":"643-51"},"PeriodicalIF":0.0,"publicationDate":"1982-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01542857","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18150919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17