Somatic Cell Genetics最新文献

Transformation of temperature-sensitive growth mutant of BHK21 cell line to wild-type phenotype with hamster and mouse DNA. BHK21细胞系温度敏感生长突变体转化为具有仓鼠和小鼠DNA的野生型表型。

Somatic Cell Genetics Pub Date : 1983-11-01 DOI: 10.1007/BF01539472

R Kai, T Sekiguchi, K Yamashita, M Sekiguchi, T Nishimoto

引用次数: 10

Secondary mutation resistant to 7-ketocholesterol rescues a sterol metabolic defect in amphotericin B-resistant Chinese hamster cell line. 7-酮胆固醇抗性继发突变修复了两性霉素b抗性中国仓鼠细胞系的一种固醇代谢缺陷。

Somatic Cell Genetics Pub Date : 1983-11-01 DOI: 10.1007/BF01539471

M Kuwano, A Masuda, K Hidaka, S I Akiyama

引用次数: 5

LPS-nonresponsive variants of mouse B cell lymphoma, 70Z/3: isolation and characterization. 70Z/3小鼠B细胞淋巴瘤脂多糖无应答变异体的分离与鉴定

Somatic Cell Genetics Pub Date : 1983-11-01 DOI: 10.1007/BF01539475

P E Mains, C H Sibley

引用次数: 26

Mammalian mitochondrial mutants selected for resistance to the cytochrome b inhibitors HQNO or myxothiazol. 对细胞色素b抑制剂HQNO或粘噻唑产生抗性的哺乳动物线粒体突变体。

Somatic Cell Genetics Pub Date : 1983-11-01 DOI: 10.1007/BF01539476

N Howell, A Bantel, P Huang

{"title":"Mammalian mitochondrial mutants selected for resistance to the cytochrome b inhibitors HQNO or myxothiazol.","authors":"N Howell, A Bantel, P Huang","doi":"10.1007/BF01539476","DOIUrl":"https://doi.org/10.1007/BF01539476","url":null,"abstract":"Mouse LA9 cell lines were selected for increased resistance to either HQNO or myxothiazol, inhibitors of electron transport which bind to the mitochondrial cytochrome b protein. Two phenotypically distinguishable HQNO-resistant mutants were recovered while the myxothiazol-resistant isolates had a common phenotype. All three mutant phenotypes were transmitted cytoplasmically in cybrid crosses. Biochemical studies further established that for all three mutant types, resistance at the cellular level was paralleled by an increase in inhibitor resistance of mitochondrial succinate-cytochrome c oxidoreductase, the respiratory complex containing cytochrome b. As with the previously described mitochondrial antimycin-resistant mutant, the initial biochemical and genetic studies indicated that these mutations occur within the mitochondrial cytochrome b gene. This conclusion was strongly supported by the results of mtDNA restriction fragment analyses in which it was found that one HQNO-resistant mutant had undergone a small insertion or duplication in the apocytochrome b gene. Finally, all four mitochondrial cytochrome b mutants have been analyzed in both cell plating studies and succinate-cytochrome c oxidoreductase assays to determine the pattern of cross-resistance to inhibitors of cytochrome b other than the one used for selection.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 6","pages":"721-43"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01539476","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17378916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Chromosome assignment of genes encoding the alpha and beta subunits of glycoprotein hormones in man and mouse. 编码糖蛋白激素α和β亚基的基因在人和小鼠中的染色体分配。

Somatic Cell Genetics Pub Date : 1983-11-01 DOI: 10.1007/BF01539478

S L Naylor, W W Chin, H M Goodman, P A Lalley, K H Grzeschik, A Y Sakaguchi

{"title":"Chromosome assignment of genes encoding the alpha and beta subunits of glycoprotein hormones in man and mouse.","authors":"S L Naylor, W W Chin, H M Goodman, P A Lalley, K H Grzeschik, A Y Sakaguchi","doi":"10.1007/BF01539478","DOIUrl":"https://doi.org/10.1007/BF01539478","url":null,"abstract":"The chromosomal locations of the genes for the common alpha subunit of the glycoprotein hormones and the beta subunit of chorionic gonadotropin in humans and mice have been determined by restriction enzyme analysis of DNA isolated from somatic cell hybrids. The CG alpha gene (CGA), detected as a 15-kb BamHI fragment in human DNA by hybridization to CG alpha cDNA, segregated with the chromosome 6 enzyme markers ME1 (malic enzyme, soluble) and SOD2 (superoxide dismutase, mitchondrial) and an intact chromosome 6 in human-rodent hybrids. Cell hybrids containing portions of chromosome 6 allowed the localization of CGA to the q12 leads to q21 region. The greater than 30- and 6.5-kb BamHI CGB fragments hybridizing to human CG beta cDNA segregated concordantly with the human chromosome 19 marker enzymes PEPD (peptidase D) and GPI (glucose phosphate isomerase) and a normal chromosome 19 in karyotyped hybrids. A KpnI-HindIII digest of cell hybrid DNAs indicated that the multiple copies of the CG beta gene are all located on human chromosome 19. In the mouse, the alpha subunit gene, detected by a mouse thyrotropin (TSH) alpha subunit probe, and the CG beta-like sequences (CG beta-LH beta), detected by the human CG beta cDNA probe, are on chromosomes 4 and 7, respectively.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 6","pages":"757-70"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01539478","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17639048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 104

Assignment of gene(s) coding for antigen defined by monoclonal antibody 2B2. 单克隆抗体2B2抗原编码基因的分配。

Somatic Cell Genetics Pub Date : 1983-11-01 DOI: 10.1007/BF01539477

H A Suomalainen, C Lundqvist, C G Gahmberg, J Schröder

引用次数: 6

Coreactivation of four inactive X genes in a hamster x human hybrid and persistence of late replication of reactivated X chromosome. 四种失活X基因在仓鼠与人杂交中的共同激活和重新激活的X染色体后期复制的持久性。

Somatic Cell Genetics Pub Date : 1983-11-01 DOI: 10.1007/BF01539470

M C Hors-Cayla, S Heuertz, J Frezal

引用次数: 36

Assignment of murine cellular Harvey ras gene to chromosome 7. 小鼠细胞Harvey ras基因在7号染色体上的分配。

Somatic Cell Genetics Pub Date : 1983-11-01 DOI: 10.1007/BF01539473

D D Pravtcheva, F H Ruddle, R W Ellis, E M Scolnick