Somatic Cell Genetics最新文献_第10页

Expression of human hepatic genes in somatic cell hybrids. 人肝基因在体细胞杂交中的表达。

Somatic Cell Genetics Pub Date : 1982-05-01 DOI: 10.1007/BF01538896

G J Darlington, J K Rankin, G Schlanger

{"title":"Expression of human hepatic genes in somatic cell hybrids.","authors":"G J Darlington, J K Rankin, G Schlanger","doi":"10.1007/BF01538896","DOIUrl":"https://doi.org/10.1007/BF01538896","url":null,"abstract":"Four diploid human cell types (lymphocytes, fibroblasts, amniotic fluid cells, and hepatocytes) were fused to mouse hepatoma cells, HH. HH synthesized and secreted several liver-specific gene products including albumin, transferrin, and alpha-fetoprotein. The resulting interspecific hybrids were compared to determine whether or not the pattern of human hepatic gene expression was similar when these various cells were fused with the mouse hepatoma line. The expression of six human hepatic genes was examined, including albumin, alpha-fetoprotein, ceruloplasmin, transferrin, alpha-1-antitrypsin, and haptoglobin. Albumin was most frequently expressed while alpha-fetoprotein was not detected in any of the hybrids studied. The patterns of expression of human serum proteins differed between the hybrid series. Hybrids derived from human fibroblasts produced primarily albumin, while those derived from lymphoblastoid cells and amniocytes had a higher frequency of clones secreting alpha-1-antitrypsin. The findings reported here suggest that the frequency of hybrid clones expressing human hepatic gene products and the array of proteins produced are influenced by the histogenetic state of the human parental cell type.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"8 3","pages":"403-12"},"PeriodicalIF":0.0,"publicationDate":"1982-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01538896","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17245285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Molecular mechanism of extinction of liver-specific functions in mouse hepatoma x rat fibroblast hybrids: extinction of the albumin gene. 小鼠肝癌与大鼠成纤维细胞杂交中肝脏特异性功能消失的分子机制:白蛋白基因的消失。

Somatic Cell Genetics Pub Date : 1982-05-01 DOI: 10.1007/BF01538893

J Papaconstantinou, E Wong, H Ratrie, C Szpirer, J Szpirer

引用次数: 11

Somatic cell genetic analysis of HLA-A, B, C and human beta 2-microglobulin expression. HLA-A、B、C和人β 2微球蛋白表达的体细胞遗传分析。

Somatic Cell Genetics Pub Date : 1982-05-01 DOI: 10.1007/BF01538895

M E Kamarck, J A Barbosa, F H Ruddle

引用次数: 21

Isolation of UV-sensitive mutants of mouse L5178Y cells by a cell suspension spotting method. 细胞悬液斑点法分离小鼠L5178Y细胞紫外敏感突变体。

Somatic Cell Genetics Pub Date : 1982-05-01 DOI: 10.1007/BF01538891

T Shiomi, N Hieda-Shiomi, K Sato

{"title":"Isolation of UV-sensitive mutants of mouse L5178Y cells by a cell suspension spotting method.","authors":"T Shiomi, N Hieda-Shiomi, K Sato","doi":"10.1007/BF01538891","DOIUrl":"https://doi.org/10.1007/BF01538891","url":null,"abstract":"We have isolated 56 UV-sensitive mutant clones from a mouse L51 T/t line of L5178Y cells by a cell suspension spotting method. Five mutants have also been isolated from L51 T/t and L5178Y cells by the method reported by Thompson and coworkers (22). We divided the mutants into two groups, \"highly sensitive\" and \"moderately sensitive\" mutants, according to their sensitivity to UV irradiation. Fifty-eight mutants were highly sensitive and three were moderately sensitive to UV. The reconstruction experiments indicate that more than 90% of highly sensitive mutants were recovered by the cell suspension spotting method. Frequencies of recovered mutants highly sensitive to UV increased with increasing dose of mutagens. Recovered mutant frequency reached 10(-2) after treatment with 1.5 micrograms/ml of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) (survival 0.2%). Eight UV-sensitive mutants were divided into four complementation groups. These mutants were 2-6 times more sensitive to UV than parental L51 T/t cells in terms of D37 (dose required to reduce survival to 37%). Four representative UV-sensitive mutants which are classified into different complementation groups were examined for their sensitivity to killing by UV, 4-nitroquinoline-1-oxide (4NQO), mitomycin C (MMC), X-rays, and MNNG. All four classes of mutants were found to be cross-sensitive to UV, 4NQO, and MMC, but not sensitive to X-rays and MNNG.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"8 3","pages":"329-45"},"PeriodicalIF":0.0,"publicationDate":"1982-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01538891","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18129433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Application of the BrdU/thymidine method to flow cytogenetics: differential quenching/enhancement of Hoechst 33258 fluorescence of late-replicating chromosomes. BrdU/胸苷法在流动细胞遗传学中的应用:晚期复制染色体Hoechst 33258荧光的差异猝灭/增强

Somatic Cell Genetics Pub Date : 1982-05-01 DOI: 10.1007/BF01538890

C Cremer, J W Gray

{"title":"Application of the BrdU/thymidine method to flow cytogenetics: differential quenching/enhancement of Hoechst 33258 fluorescence of late-replicating chromosomes.","authors":"C Cremer, J W Gray","doi":"10.1007/BF01538890","DOIUrl":"https://doi.org/10.1007/BF01538890","url":null,"abstract":"Discrimination between many types of isolated mammalian chromosomes can be accomplished by dual-beam flow cytometry following DNA staining with Hoechst 33258 (HO) and Chromomycin A3 (CA3). In this report, we show that the bivariate discrimination of selected late-replicating Chinese hamster M3-1 chromosomes can be improved by appropriate treatment of the cells with 5-bromo-2'-deoxyuridine (BrdU) prior to chromosome isolation and staining. Two labeling schemes are reported. In one scheme the chromosomes are collected from cells labeled with BrdU only during late S phase. The Hoechst fluorescence of the 10, 11, M2, and Y chromosomes is substantially quenched by the incorporated BrdU, thus improving their discrimination. In the other scheme, chromosomes are collected from cells labeled with thymidine (dT) during late S phase following 20 h of growth in BrdU-containing medium. The Hoechst fluorescence of the 10, 11, M2, and Y chromosomes is quenched less than the other chromosomes, again improving their discrimination. Y chromosomes from chromosome suspensions of untreated controls, of cells labeled with BrdU during late S phase, and of cells labeled with dT during late S phase following 20 h growth in BrdU were separated by dual-parameter sorting. While the purity of the sorted Y chromosome was 15% in untreated controls, it was 70-75% using the BrdU/dT labeling protocols.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"8 3","pages":"319-27"},"PeriodicalIF":0.0,"publicationDate":"1982-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01538890","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17245281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

A temperature-sensitive DNA synthesis mutant isolated from the Chinese hamster ovary cell line. 从中国仓鼠卵巢细胞系中分离的温度敏感DNA合成突变体。

Somatic Cell Genetics Pub Date : 1982-03-01 DOI: 10.1007/BF01538676

A A McCracken

引用次数: 9

Assignment of an oligomycin-resistance locus to human chromosome 10. 人类第10号染色体上寡霉素耐药位点的分配。

Somatic Cell Genetics Pub Date : 1982-03-01 DOI: 10.1007/BF01538679

K A Webster, N A Oliver, D C Wallace

引用次数: 11

Transcriptional control of the expression of mouse globin genes in myeloma x erythroleukemia cell hybrids. 骨髓瘤与红白血病杂交细胞中小鼠珠蛋白基因表达的转录调控。

Somatic Cell Genetics Pub Date : 1982-03-01 DOI: 10.1007/BF01538674

A Ar-Rushdi, K B Tan, C M Croce