人类第10号染色体上寡霉素耐药位点的分配。

K A Webster, N A Oliver, D C Wallace
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引用次数: 11

摘要

分离出一种寡霉素耐药人纤维肉瘤HT1080,其特征为核共显性。该突变体稳定,对呼吸抑制剂无交叉耐药,并且含有对寡霉素不敏感的线粒体atp酶。在人类突变体和小鼠细胞系之间形成的杂种表达了抗性表型。通过胰蛋白酶-吉姆萨显带对这些杂交种进行详细的核型分析,发现对寡霉素的抗性与两条人类染色体的保留有关。通过线粒体合成蛋白和线粒体DNA的分析表明,杂交系只含有小鼠线粒体DNA。该研究将ATPase寡霉素抗性位点分配到人类10号染色体上,并表明小鼠和人类亚基可以结合在一个功能性酶复合体中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assignment of an oligomycin-resistance locus to human chromosome 10.

An oligomycin-resistant variant of human fibrosarcoma HT1080 was isolated and characterized as nuclear and codominant. The mutant was stable, was not cross-resistant to respiratory inhibitors, and it contained a mitochondrial ATPase which was less sensitive to oligomycin. Hybrids formed between the human mutant and a mouse cell line expressed the resistance phenotype. By a detailed karyotypic analysis of these hybrids using trypsin-Giemsa banding it was found that resistance to oligomycin correlated with the retention of two human chromosomes 10. The hybrid lines contained only mouse mitochondrial DNA as shown by analyses of mitochondrially synthesized proteins and mitochondrial DNA. The study assigns an ATPase oligomycin-resistance locus to human chromosome 10 and suggests that mouse and human subunits can combine in a functional enzyme complex.

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