{"title":"Somatic cell genetic analysis of HLA-A, B, C and human beta 2-microglobulin expression.","authors":"M E Kamarck, J A Barbosa, F H Ruddle","doi":"10.1007/BF01538895","DOIUrl":null,"url":null,"abstract":"<p><p>We have examined the cell surface expression of the human histocompatibility antigens HLA-A, B, C and beta 2-microglobulin (beta 2m) on a human-mouse somatic cell hybrid line. Using specific antibodies and the fluorescence-activated cell sorter (FACS), we viably fractionated and characterized four separate hybrid subpopulations (HLA+,beta 2m+; HLA+,beta 2m-; HLA-,beta 2m+; HLA-,beta 2m-). Hybrid selection based on surface antigen expression resulted in corresponding genetic selection for and against human chromosomes 6 and 15. Studies of the homogeneous hybrid sublines revealed that the presence of human beta 2m in a hybrid cell dramatically increased the surface expression of human HLA-A, B, C and mouse H-2Kk antigens. The results demonstrate the importance of human chromosome-specific surface markers and the fluorescence-activated cell sorter in somatic cell genetic analysis.</p>","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"8 3","pages":"385-402"},"PeriodicalIF":0.0000,"publicationDate":"1982-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01538895","citationCount":"21","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Somatic Cell Genetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/BF01538895","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 21
Abstract
We have examined the cell surface expression of the human histocompatibility antigens HLA-A, B, C and beta 2-microglobulin (beta 2m) on a human-mouse somatic cell hybrid line. Using specific antibodies and the fluorescence-activated cell sorter (FACS), we viably fractionated and characterized four separate hybrid subpopulations (HLA+,beta 2m+; HLA+,beta 2m-; HLA-,beta 2m+; HLA-,beta 2m-). Hybrid selection based on surface antigen expression resulted in corresponding genetic selection for and against human chromosomes 6 and 15. Studies of the homogeneous hybrid sublines revealed that the presence of human beta 2m in a hybrid cell dramatically increased the surface expression of human HLA-A, B, C and mouse H-2Kk antigens. The results demonstrate the importance of human chromosome-specific surface markers and the fluorescence-activated cell sorter in somatic cell genetic analysis.
我们检测了人类组织相容性抗原HLA-A、B、C和β 2微球蛋白(β 2m)在人-鼠体细胞杂交细胞系上的细胞表面表达。利用特异性抗体和荧光活化细胞分选器(FACS)，我们对4个不同的杂交亚群(HLA+，beta 2m+;HLA +β2 m -;HLA -、β2 m +;HLA -、β2 m -)。基于表面抗原表达的杂交选择导致了6号染色体和15号染色体对应的遗传选择。对同质杂交亚系的研究表明，人β 2m在杂交细胞中的存在显著增加了人HLA-A、B、C和小鼠H-2Kk抗原的表面表达。结果证明了人类染色体特异性表面标记和荧光激活细胞分选器在体细胞遗传分析中的重要性。
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