Somatic Cell Genetics最新文献_第4页

Differentiation is not restored in hybrids between independent variants of a rat hepatoma. 在大鼠肝癌的独立变体之间的杂交中，分化不恢复。

Somatic Cell Genetics Pub Date : 1983-05-01 DOI: 10.1007/BF01539147

J Levilliers, M C Weiss

引用次数: 4

Human and mouse cellular myc protooncogenes reside on chromosomes involved in numerical and structural aberrations in cancer. 人和小鼠细胞myc原癌基因驻留在参与癌症数量和结构畸变的染色体上。

Somatic Cell Genetics Pub Date : 1983-05-01 DOI: 10.1007/BF01539146

A Y Sakaguchi, P A Lalley, S L Naylor

{"title":"Human and mouse cellular myc protooncogenes reside on chromosomes involved in numerical and structural aberrations in cancer.","authors":"A Y Sakaguchi, P A Lalley, S L Naylor","doi":"10.1007/BF01539146","DOIUrl":"https://doi.org/10.1007/BF01539146","url":null,"abstract":"A molecular clone of viral myc (v-myc), the oncogene of avian myelocytomatosis virus, MC29, detected homologous human, mouse, and Chinese hamster cellular myc (c-myc) sequences by Southern filter hybridization. A v-myc probe, containing sequences from the 3' domain of the gene, hybridized to single human HindIII and mouse EcoRI genomic DNA fragments of the cellular myc genes whose segregation could be followed in interspecies somatic cell hybrids. Human c-myc segregated concordantly with the enzyme marker glutathione reductase and with a karyotypically normal chromosome 8. A rearrangement of human c-myc was observed in Burkitt's lymphoma cells possessing the t(8;14) translocation. These results suggest that human c-myc is located close to the breakpoint on chromosome 8 (q24) involved in the t(8;14) translocation. The mouse c-myc gene segregated concordantly with chromosome 15 in mouse-Chinese hamster cell hybrids. These gene assignments are noteworthy, as structural and numerical abnormalities of human chromosome 8 and mouse chromosome 15 are associated frequently with B-cell neoplasms.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 3","pages":"391-405"},"PeriodicalIF":0.0,"publicationDate":"1983-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01539146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17907597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Purification of cybrids by fluorescence-activated cell sorting. 荧光活化细胞分选纯化杂交体。

Somatic Cell Genetics Pub Date : 1983-05-01 DOI: 10.1007/BF01539145

T Kliot-Fields, D A Finney, A Wiseman

引用次数: 2

Chromosome mapping of human cell surface molecules: monoclonal anti-human lymphocyte antibodies 4F2, A3D8, and A1G3 define antigens controlled by different regions of chromosome 11. 人细胞表面分子的染色体定位:单克隆抗人淋巴细胞抗体4F2、A3D8和A1G3定义由11号染色体不同区域控制的抗原。

Somatic Cell Genetics Pub Date : 1983-05-01 DOI: 10.1007/BF01539142

U Francke, B E Foellmer, B F Haynes

{"title":"Chromosome mapping of human cell surface molecules: monoclonal anti-human lymphocyte antibodies 4F2, A3D8, and A1G3 define antigens controlled by different regions of chromosome 11.","authors":"U Francke, B E Foellmer, B F Haynes","doi":"10.1007/BF01539142","DOIUrl":"https://doi.org/10.1007/BF01539142","url":null,"abstract":"Monoclonal antibodies 4F2, A3D8, and A1G3, directed against cell surface antigens present on subsets of human cells, were used to identify the human chromosome regions that code for the antigenic determinants. Human fibroblasts expressed all three antigens, and no cross-reactivity with Chinese hamster or mouse cells was found. Fourteen rodent X human somatic cell hybrids, derived from six different human donors and from two different Chinese hamster and one mouse cell line, were studied simultaneously for human chromosome content and for antibody binding as detected by indirect immunofluorescence. Concordancy with binding of all three antibodies was observed only for human chromosome 11. All other chromosomes were excluded by three or more discordant hybrid clones. Data from six hybrids containing three different regions of chromosome 11 indicate that it is the long arm of chromosome 11 which is both necessary and sufficient for expression of the human antigen defined by 4F2 while the antigen(s) defined by A3D8 and A1G3 map to short arm.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 3","pages":"333-44"},"PeriodicalIF":0.0,"publicationDate":"1983-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01539142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17254950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Evidence for a trans-dominant regulator of purine nucleoside phosphorylase expression in rat hepatoma cells. 大鼠肝癌细胞中嘌呤核苷磷酸化酶表达的反显性调节因子的证据。

Somatic Cell Genetics Pub Date : 1983-03-01 DOI: 10.1007/BF01543180

P A Hoffee, S W Hunt, J Chiang, M C Labant, M Clarke, P Jargiello

{"title":"Evidence for a trans-dominant regulator of purine nucleoside phosphorylase expression in rat hepatoma cells.","authors":"P A Hoffee, S W Hunt, J Chiang, M C Labant, M Clarke, P Jargiello","doi":"10.1007/BF01543180","DOIUrl":"https://doi.org/10.1007/BF01543180","url":null,"abstract":"Purine nucleoside phosphorylase (PNP) levels are modulated during the growth cycle of rat hepatoma cells and increase two- to three-fold as cells go from early exponential growth phase to stationary growth phase. A mutant of these hepatoma cells has been isolated which is deficient in PNP activity. Quantitative immunoprecipitation tests indicate that the decrease in enzyme activity is due to a decrease in the number of PNP molecules. The low level of PNP enzyme produced by the mutant, however, is indistinguishable from the wild-type enzyme, suggesting that the mutant may be defective in the ability to modulate PNP levels. Fusion of the mutant cells to wild-type parental cells results in hybrids that express the mutant phenotype. Segregants that arise from the hybrids show chromosome loss and reexpression of the wild-type parental phenotype, the mutant parental phenotype, and a 2S wild-type phenotype. These indicate the following about the defect in modulation in the mutant PNP-100: (1) it is trans dominant to the wild-type; (2) its effect is negative; (3) some genomic element is required for its continued effect; and (4) it does not act by obliterating its functioning counterpart in hybrid cells.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 2","pages":"249-67"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01543180","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17463815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mitochondrial genetics of mammalian cells: a mouse antimycin-resistant mutant with a probable alteration of cytochrome b. 哺乳动物细胞的线粒体遗传学:具有细胞色素b可能改变的小鼠抗霉素抗性突变体。

Somatic Cell Genetics Pub Date : 1983-03-01 DOI: 10.1007/BF01543174

N Howell, P Huang, K Kelliher, M L Ryan

{"title":"Mitochondrial genetics of mammalian cells: a mouse antimycin-resistant mutant with a probable alteration of cytochrome b.","authors":"N Howell, P Huang, K Kelliher, M L Ryan","doi":"10.1007/BF01543174","DOIUrl":"https://doi.org/10.1007/BF01543174","url":null,"abstract":"Mouse LA9 antimycin-resistant mutants (ANT-R) were isolated and characterized. Genetic analyses established that this phenotype is encoded within the mtDNA: (1) the ANT-R phenotype showed frequent mitotic segregation and reassortment in hybrid clonal lines; (2) it was transmitted directly in cybrid crosses; and (3) it was cotransmitted in cybrid crosses with the mitochondrial CAP-R marker. Furthermore, the genetic studies suggested that the LA9 CAP-R ANT-R cells were heteroplasmic and contained at least two mtDNA genotypes, cap-r ant-s and cap-s ant-r. Cellular respiration of the ANT-R mutant was markedly more resistant to inhibition by antimycin than that of the parental ANT-S cells. The increased resistance of cellular respiration was entirely accounted for by an increase in the resistance of mitochondrial succinate-cytochrome c oxidoreductase to antimycin inhibition. There was no detectable change in the specific activity of the oxidoreductase in mitochondria of resistant ANT-R cells nor in the sensitivity of the complex to three other specific inhibitors of the complex: TTFA, myxothiazol, and HQNO. Taken together, these studies indicate that the ANT-R phenotype is most likely encoded within the mitochondrial cytochrome b gene and, more specifically, within an antimycin binding domain.","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 2","pages":"143-63"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01543174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17362734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Isolation of cell cycle-dependent gamma ray-sensitive Chinese hamster ovary cell. 细胞周期依赖性γ射线敏感的中国仓鼠卵巢细胞的分离。

Somatic Cell Genetics Pub Date : 1983-03-01 DOI: 10.1007/BF01543175

T D Stamato, R Weinstein, A Giaccia, L Mackenzie

引用次数: 176

Isolation of mutants of CHO cells resistant to 6 (p-hydroxyphenylazo)-uracil II. Mutants auxotrophic for deoxypyrimidines. 6(对羟基苯基偶氮)-尿嘧啶抗CHO细胞突变体的分离。脱氧嘧啶的突变体营养不良。

Somatic Cell Genetics Pub Date : 1983-03-01 DOI: 10.1007/BF01543182

E Arpaia, P N Ray, L Siminovitch

引用次数: 5

Isolation of mutants of CHO cells resistant to 6(p-hydroxyphenylazo)-uracil I. A novel BrdU cross-resistant phenotype. 6(对羟基苯基偶氮)-尿嘧啶抗CHO细胞突变体的分离ⅰ。一种新的BrdU交叉抗性表型。

Somatic Cell Genetics Pub Date : 1983-03-01 DOI: 10.1007/BF01543181

E Arpaia, P N Ray, L Siminovitch