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Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica最新文献

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[An efficient approach to the synthesis of a high-quality random peptide repertoire]. 一种合成高质量随机肽库的有效方法。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-10-01
Chang-Cheng Yin, Shang-Zi Wang, Zheng-Hong Lin, Xiang-Bin Wang, Jing Liu, Hua-Liang Huang
{"title":"[An efficient approach to the synthesis of a high-quality random peptide repertoire].","authors":"Chang-Cheng Yin,&nbsp;Shang-Zi Wang,&nbsp;Zheng-Hong Lin,&nbsp;Xiang-Bin Wang,&nbsp;Jing Liu,&nbsp;Hua-Liang Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>During the construction of a random peptide repertoire using degenerate models, unexpected amino acids or stop codons are almost unavoidable. To conquer this shortcoming, a new split-mix-split method of oligonucleotide synthesis was developed. A 13-amino acids random peptide library had been constructed by using this method. The sequencing results of 16 clones indicated that neither stop codon nor codon for cysteine appeared as designed. The occurrence rations of 19 amino acids were also calculated and no obvious amino acid bias had been observed. By using this method, the type and quantity of amino acid at certain position of a peptide could be controlled well, so this split-mix-split method, combined with degenerate could meet the needs of a high diversity random peptide library.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40828249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of a strong repression domain in the hinge region of orphan nuclear receptor hB1F/hLRH-1. 孤儿核受体hB1F/hLRH-1铰链区强抑制结构域的表征。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-10-01
Ping-Long Xu, Shi-Fang Shan, Yu-Ying Kong, You-Hua Xie, Yuan Wang
{"title":"Characterization of a strong repression domain in the hinge region of orphan nuclear receptor hB1F/hLRH-1.","authors":"Ping-Long Xu,&nbsp;Shi-Fang Shan,&nbsp;Yu-Ying Kong,&nbsp;You-Hua Xie,&nbsp;Yuan Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Human hepatitis B virus enhancer II B1 binding factor (hB1F also known as NR5A2, LRH-1, FTF or CPF) is an orphan nuclear receptor and belongs to the fushi tarazu factor I (FTZ-F1) subfamily. It plays important roles in the transcriptional regulation of a number of genes involved in bile acid biosynthesis pathway, hepatitis B virus (HBV) replication and liver specific regulatory network. Like other nuclear receptors, hB1F is composed of modular functional domains. We characterized a domain in its hinge region that imposes a strong repression on the transcriptional activity of hB1F, which is important for the function of hB1F on regulating the activity of HBV enhancer II/core promoter. Mutations of the core residues in this domain abrogate the repression. Bioinformatic analysis reveals that the amino acid sequence of this region is highly conserved only among members of the FTZ-F1 subfamily. The repression is observed in five cell lines tested, while the degree of the repression varies greatly, which does not parallel with the expression level of the DEAD box protein of 130 kD (DP103), a potential interacting protein of a homologous domain in the steroidogenic factor 1 (SF-1). Moreover, the repression is not affected by the silencing mediator for retinoic acid receptor and thyroid hormone receptor (SMRT) and steroid receptor coactivator 1 (SRC-1). Collectively, these data suggest a novel regulatory mechanism for the transcriptional activity of hB1F.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40828247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[An improved solid phase synthesis of human proinsulin C-peptide]. 一种改进的人胰岛素原c肽固相合成方法。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-10-01
Jia-Hao Shi, Jiang Li, Cai-E Ju, Da-Fu Cui
{"title":"[An improved solid phase synthesis of human proinsulin C-peptide].","authors":"Jia-Hao Shi,&nbsp;Jiang Li,&nbsp;Cai-E Ju,&nbsp;Da-Fu Cui","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Human proinsulin C-peptide with C-terminal glutamine amide could be prepared through solid phase method by combining the gamma-carboxyl group of glutamic acid with the amino group of MBHA resin. The protecting groups were cleaved by HF. MBHA resin is relatively inexpensive. The new method is another way for the preparation of human proinsulin C-peptide. The preparation human proinsulin C-peptide of analogue using of PAM resin was also reported.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40828248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Current strategies and future directions of antiangiogenic tumor therapy. 抗血管生成肿瘤治疗的当前策略和未来方向。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-10-01
Zi-Lai Zhang, Jin-Hui Wang, Xin-Yuan Liu
{"title":"Current strategies and future directions of antiangiogenic tumor therapy.","authors":"Zi-Lai Zhang,&nbsp;Jin-Hui Wang,&nbsp;Xin-Yuan Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Neovascularization is a prerequisite for progressive growth of most tumors and their metastases. Therefore, inhibition of angiogenesis could be one of the most promising strategies that might lead to the development of novel anticancer therapy. New blood vessels forming in tumors can be avoided by interfering the process of angiogenesis through suppressing the proangiogenic signal or augmenting the antiangiogenic factors. Concentrated efforts in this area have lead to the discovery of many proangiogenic factors as well as their inhibitors, and antiangiogenic factors. For the established tumor vasculature, tumor vasculature-targeted delivery of antiangiogenic substances and endothelial cell vaccines has been explored. Although some antiangiogenic drugs are currently in clinical development, for future reason, more efficient therapeutic methods, including antiangiogenic gene therapy strategy, targeted drug delivery system, and the combination of antiangiogenic agents with immunotherapy, chemotherapy or radiotherapy are being explored. With the development of tumor model assessment system, clinical use of the above antiangiogenic tumor therapy methods could be achieved.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40828308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cre-mediated site-specific cassette exchange in erythroid cell. 红细胞中cre介导的位点特异性盒体交换。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-10-01
Xing-Guo Li, Hao-Heng Yan, De-Pei Liu, De-Long Hao, Chih-Chuan Liang
{"title":"Cre-mediated site-specific cassette exchange in erythroid cell.","authors":"Xing-Guo Li,&nbsp;Hao-Heng Yan,&nbsp;De-Pei Liu,&nbsp;De-Long Hao,&nbsp;Chih-Chuan Liang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cre-mediated cassette exchange has been developed to perform site-specific chromosomal integration using Cre recombinase. Here, site-specific integration with inverted Lox sites was used to investigate the erythroid cis-acting DNA element in specific chromatin contexts in mouse erythroleukemia cells. Single hygromycin-resistant clones were obtained from the selective semi-solid medium containing hygromycin post-electroporation. PCR and Southern blotting analysis showed single-copy integration of target vector in clones A, B and D. Site-specific cassette exchange was performed in clone A with exchange vector and Cre expression plasmid, followed by gancyclovir selection. Flow cytometry was used for analysis of EGFP gene expression. A 732-bp fragment of human beta-globin gene cluster 5' DNase I hypersensitive site 2 (HS2) was exchanged and integrated into clone A in an anti-genomic orientation. The low EGFP expression in clone A-HS may be due to the orientation-dependent gene silencing caused by integration of HS2 in a non-permissive orientation.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40907144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DNA sequencing of a plasmid with virulence from marine fish pathogen Vibrio anguillarum. 海鱼致病菌鳗弧菌毒力质粒DNA序列分析。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-10-01
Hai-Zhen Wu, Hui-Zhan Zhang, Cai-Xia Lü, Na Liang, Hui-Yi Jin, Yue Ma, Yuan-Xing Zhang
{"title":"DNA sequencing of a plasmid with virulence from marine fish pathogen Vibrio anguillarum.","authors":"Hai-Zhen Wu,&nbsp;Hui-Zhan Zhang,&nbsp;Cai-Xia Lü,&nbsp;Na Liang,&nbsp;Hui-Yi Jin,&nbsp;Yue Ma,&nbsp;Yuan-Xing Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>DNA sequence of a plasmid pEIB1 associated with virulence from the marine fish pathogen Vibrio anguillarum was determined using the methods of restriction endonuclease digestion, subcloning, and primer walking. The whole length of obtained pEIB1 DNA sequence was 66 164 bp, and the overall G+C content of DNA sequence is 42.7%. This sequence encodes 44 open reading frames containing the genes of DNA replication, biosynthesis and regulation of the siderophore anguibactin and transport of ferric-anguibactin complexes.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40907146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition of replication of goose paramyxovirus SF02 by hammerhead ribozyme targeting to the SF02 F mRNA in chicken embryo fibroblasts. 锤头核酶对鹅副粘病毒SF02在鸡胚成纤维细胞中复制的抑制作用
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Jian Zou, Zun-Xun Gong
{"title":"Inhibition of replication of goose paramyxovirus SF02 by hammerhead ribozyme targeting to the SF02 F mRNA in chicken embryo fibroblasts.","authors":"Jian Zou,&nbsp;Zun-Xun Gong","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hammerhead ribozyme RzF598 and its dysfunctional mutant dRzF598 targeting to the (F) gene of goose paramyxovirus SF02 have been designed. The transgenic plasmids pcDNA-RzF598 and pcDNA-dRzF598 were constructed by inserting ribozyme genes into eukaryotic expression vector pcDNA3. The plasmid pcDNA3 that lacks full ribozyme gene was used as a control. Plasmids pcDNA-RzF598, pcDNA-dRzF598 and pcDNA3 were transfected into chicken embryo fibroblasts (CEFs). The concentration of virus released by infected CEFs and the survival percentages of CEFs were identified. The results indicated that RzF598 successfully suppressed the replication of SF02 in CEFs. Survival percentage of CEFs being transfected with pcDNA-RzF598 and infected SF02 was up to 78.8%, while the survival percentages of untransfected CEFs and CEFs transfected with pcDNA3 after infection with SF02 were only about 5%.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Parabolic bursting induced by veratridine in rat injured sciatic nerves. 缬曲啶对大鼠坐骨神经损伤的抛物性破裂作用。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Yong Xie, Yu-Bin Duan, Jian-Xue Xu, Yan-Mei Kang, San-Jue Hu
{"title":"Parabolic bursting induced by veratridine in rat injured sciatic nerves.","authors":"Yong Xie,&nbsp;Yu-Bin Duan,&nbsp;Jian-Xue Xu,&nbsp;Yan-Mei Kang,&nbsp;San-Jue Hu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A specific bursting, parabolic bursting induced by veratridine, has been observed in rat injured sciatic nerve. With the help of Plant model, the biophysical mechanism for such a phenomenon is revealed from the viewpoint of nonlinear dynamical theory. The slow sodium influx educed by veratridine and the calcium-dependent potassium outflux are regarded as the two slow variables, which are responsible for the parabolic bursting. Furthermore, the roles that veratridine plays in the emergence of the parabolic bursting, namely inhibiting the inactivation of sodium channels and eliciting the slow sodium influx, are clarified.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Involvement of dopamine D3 and neuropeptide Y Y5 receptors in diabetic gastroparetic rats without response to erythromycin. 多巴胺D3和神经肽yy5受体参与糖尿病胃轻瘫大鼠对红霉素无反应。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Xin-Yu Qin, Zhi-Gang Wang, Jian Fei, Feng-Lin Liu, Da-Fu Cui, Shao-Liang Chen
{"title":"Involvement of dopamine D3 and neuropeptide Y Y5 receptors in diabetic gastroparetic rats without response to erythromycin.","authors":"Xin-Yu Qin,&nbsp;Zhi-Gang Wang,&nbsp;Jian Fei,&nbsp;Feng-Lin Liu,&nbsp;Da-Fu Cui,&nbsp;Shao-Liang Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Erythromycin may accelerate gastric emptying in animals and human probably as an motilin agonist, but its prokinetic effects show obvious individual disparity. This study was to find the mechanism of this phenomenon. Microarray analysis was used to screen genes that might be involved in the response of diabetic gastroparesis rats to erythromycin. It was found that erythromycin accelerated gastric emptying of diabetic rats with great individual disparity. Through microarray analysis we screened differential expression genes that might be involved in the effect of erythromycin. Among 10 genes screened out, dopamine D3 receptor (DRD3) and neuropeptide Y Y5 receptor (NPYY5) genes were submitted to RT-PCR quantification and showed consistent results with microarray. It can be concluded that erythromycin promote gastric emptying of gastroparetic rats; DRD3 and NPYY5 may be involved in prokinetic action of erythromycin; and targets other than motilin receptor of erythromycin might exist as prokinetics.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii]. [一种新型核糖体失活肽trichokirin-S1的纯化和鉴定]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Feng Li, Xin-Xiu Yang, Wei-Guo Hu, Hen-Chuan Xia, Zhen Li, Zu-Chuan Zhang
{"title":"[Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii].","authors":"Feng Li,&nbsp;Xin-Xiu Yang,&nbsp;Wei-Guo Hu,&nbsp;Hen-Chuan Xia,&nbsp;Zhen Li,&nbsp;Zu-Chuan Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A novel peptide from the seeds of Trichosanthes kirilowii, trichokirin-S1, was purified by extraction of protein body, ammonia sulfate precipitation, Blue-gel affinity chromatography, FPLC Mono S ion exchange chromatography and Superose12 gel filtration chromatography. Its molecular weight was determined to be 11,426 by MALDI-TOF MS analysis. Its reaction mechanism to inactive ribosome was the same as that of the ribosome-inactivating protein trichosanthin, a rRNA N-glycosidase. The purified trichokirin-S1 showed a strong inhibitory activity on protein synthesis in cell-free rabbit reticulocyte lysate system, with IC(50) of 0.7 nmol/L. Therefore, trichokirin-S1 may be a promising and efficient toxin moiety of immunotoxins.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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